Method used for extracting high purity vincristine from cultured catharanthus roseus

[0020] Take 5kg of activated sludge, 2.3kg of 40-mesh corn stalk powder, 1kg of glucose, 0.5kg of enol-pyruvate phosphate, 1kg of tryptophan with a concentration of 80mg / L and 0.2kg of methyl jasmonate in a container, and stir evenly; Put the mixture obtained above into the fermentation tank, set the temperature at 30°C, seal and stir the fermentation for 5 hours to remove the fermentation liquid, use the fermented material as the culture substrate, spread it evenly in the culture tank with a thickness of 8cm, and plant 2 pairs of true rice per cubic meter. The periwinkle leaves are moved into the culture tank for cultivation, the temperature is maintained at 20°C, the water content of the culture medium is 20%, and the mixed solution is sprayed every other day. The mixed solution is 8% hydrochloric acid according to the mass fraction per liter. Add 84g6-furfuryl aminopurine to the solution and mix it evenly. The amount of spraying is to spray 4mL of the mixed solution on each ...

[0023]Get 5.5kg of activated sludge, 2.3kg of 60 mesh corn stalk powder, 0.8kg of glucose, 0.5kg of enol-pyruvate phosphate, 0.8kg of 100mg / L tryptophan and 0.1kg of methyl jasmonate into the container. Stir evenly; put the above-mentioned mixture into the fermentation tank, set the temperature at 35°C, seal and stir for 7 hours to remove the fermentation liquid, use the fermented product as a culture medium, spread it evenly in the culture tank with a thickness of 10cm, and calculate it per cubic meter Plant 4 pairs of true leaves of periwinkle, move into the culture tank for cultivation, keep the temperature at 33°C, and the water content of the culture medium is 35%, and spray the mixed solution every 2 days, and the mixed solution is according to the mass fraction per liter of 8% hydrochloric acid solution is added into 84g6-furfuryl aminopurine and uniformly mixed, and the spraying amount is to spray 5mL of the mixed solution on each periwinkle, and when the periwinkle bea...

[0026] Take 5.2kg of activated sludge, 2.3kg of 40-60 mesh corn stalk powder, 0.9kg of glucose, 0.5kg of phosphate enol-pyruvate, 0.9kg of 90mg / L tryptophan and 0.2kg of methyl jasmonate into the container and stir evenly; put the mixture obtained above into a fermentation tank, set the temperature at 32°C, seal and stir for 6 hours to remove the fermentation liquid, use the fermented product as a culture medium, spread it evenly in the culture tank with a thickness of 9cm, and press Plant 3 pairs of true leaves of periwinkle per cubic meter, move it into the culture pool and cultivate it, keep the temperature at 25°C, and the water content of the culture substrate is 25%, spray the mixed solution every other day, and the mixed solution is based on the mass per liter The hydrochloric acid solution that fraction is 8% is added 84g6-furfuryl aminopurine and is evenly mixed to form, and its spraying amount is that every periwinkle sprays 5mL mixed solution, when periwinkle bears f...