Application of lupenone to preparation of drug for prevention and/or treatment of senile dementia and preparation

[0026] 1. Lupenone on Aβ 25-35 Improvement of protein-induced symptoms in senile dementia mice

[0027] 1.1 Experimental animals and reagents

[0028] Experimental animals: 50 clean-grade healthy male Kunming mice, weighing 26-30 g, were purchased from the Comparative Medicine Center of Yangzhou University. Reagent: lupenone (purity is 91%), provided by Jiangsu Kangyuan Pharmaceutical Co., Ltd.; Aβ 25-35 Purchased from sigma company; ChAT and AchE detection kits were purchased from Nanjing Jiancheng Bioengineering Institute; Naofukang was purchased from Hubei Huazhong Pharmaceutical Co., Ltd.

[0029] 1.2 Condensed Aβ 25-35 preparation of

[0030] 1mgAβ 25-35 Dissolve in 943μl sterilized physiological saline at a concentration of 1mmol / L, seal it and place it in a 37℃ cell culture incubator for 5-7d incubation to make it into condensed Aβ 25-35 , placed in a -20°C freezer for later use.

[0031] 1.3 Preparation and administration of mouse AD model

[0032] After the mi...

[0053] 2. Effect of lupenone on D-galactose and AlCl 3 Protective effect of induced compound senile dementia in rats

[0054] 2.1 Experimental animals and reagents

[0055] Experimental animals: aged healthy male Wistar rats, weighing 350-390 g, a total of 75 were purchased from the Comparative Medicine Center of Yangzhou University. Reagent: lupenone (purity is 91%), provided by Jiangsu Kangyuan Pharmaceutical Co., Ltd.; D-galactose, AlCl 3 Bought from Sinopharm Chemical Reagent Co., Ltd.; SOD, MDA, and CAT activity detection kits were purchased from Nanjing Jiancheng Bioengineering Research Institute; Naofukang was purchased from Hubei Huazhong Pharmaceutical Co., Ltd.

[0056] 2.2 Preparation and administration of compound senile dementia animal model

[0057] Experimental Wistar rats were randomly divided into control group, model group, Naofukang group, lupenone I group, and lupenone II group, with 15 rats in each group. The model group and the treatment group were gi...