Lentinan-modified carbon nano-tube as well as preparation method and application thereof

[0042] Embodiment 1 Preparation of lentinan carbon nanotubes

[0043] Add 30ml of thionyl chloride and 0.7g of carboxylated carbon nanotubes into the distillation flask, then sonicate for 10min, add 1ml of DMF and sonicate for 3min, reflux at 70°C for 10h (sonication for 10min every 2h), vacuum distillation at 73°C for 1-2h, vacuum at 50°C Dry (ventilate every 3h) until completely dry to obtain acetylated carbon nanotubes, dissolve the acetylated carbon nanotubes with 30ml DMF, add 0.2g lentinan pretreated at 120°C for 2h into DMF, ultrasonicate for 30min, add 1ml Pyridine was sonicated for 3 minutes, and the temperature was controlled at 110°C for 24 hours (sonication for 10 minutes every 3 hours), cooled to room temperature, centrifuged and poured, washed with DMAC / LICL (95 / 5) solution for 5 times, washed twice with PBS, and washed with warm water 5 times , freeze-dried, and stored in a dry place at low temperature.

[0044] Depend on figure 1 The transmission electron mic...

[0045] Example 2 Lentinan carbon nanotube immune enhancement activity comparison

[0046] Taking the lentinan carbon nanotubes (L-MWCNTs) prepared in Example 1 as the research object, using lentinan (lentinan) and carboxyl carbon nanotubes (C-MWCNTs) as the contrast, measured their immunity to the model antigen (OVA). The influence of IgG antibody level, antibody subtype, activation of lymph node dendritic cells, OVA antigen and cytokines IFN-γ, TNF-α, IL-12p70, IL-4 and IL-17 in lymph nodes.

[0047] (1) Animal grouping and handling

[0048] Sixty Blab / c mice aged 6-8 weeks were randomly divided into 5 groups, namely lentinan carbon nanotube group (L-MWCNTs), lentinan group (lentinan), carboxyl carbon nanotube group (C-MWCNTs ), the positive control group (FCA) and the PBS control group (PBS). Mice were immunized subcutaneously three times with a one-week interval between each immunization. 7, 14, 21, and 28 days after the first immunization, the IgG antibody levels, antib...