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Compound lactic acid bacterium micro-ecology liquid preparation for healthy dairy cow breeding

A technology for compounding lactic acid bacteria and liquid preparations is applied in the directions of microorganism-based methods, microorganisms, bacteria, etc., which can solve the problems such as the decrease in the number of viable bacteria, the lack of a bacterial strain library, and the difficulty in wide application, so as to improve stability, improve milk quality, The effect of reducing the number of somatic cells

Active Publication Date: 2016-09-21
金华银河生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main manifestations are: limited strain resources, lack of professional strain bank, and most of the strains have poor probiotic functions; Bacillus and yeast are mainly used, while lactic acid bacteria that can really colonize the intestinal tract are rarely used; the products mainly use It exists in solid form, but there are few liquid probiotics. Currently, the performance of liquid probiotics on the market is unstable, and the number of viable bacteria drops significantly under normal temperature conditions (generally, the mortality rate of viable bacteria after 1 month of storage is > 10%). The function of the product is limited and it is difficult to be widely used

Method used

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  • Compound lactic acid bacterium micro-ecology liquid preparation for healthy dairy cow breeding
  • Compound lactic acid bacterium micro-ecology liquid preparation for healthy dairy cow breeding
  • Compound lactic acid bacterium micro-ecology liquid preparation for healthy dairy cow breeding

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Embodiment 1

[0037] Embodiment 1: A kind of microecological liquid preparation and preparation method thereof

[0038] A compound lactic acid bacteria micro-ecological liquid preparation, the micro-ecological liquid preparation is composed of a plant Lactobacillus (Lactobacillus.plantarum) SCI-01 and a plant Lactobacillus (Lactobacillus.plantarum) SCI-02 through symbiotic coupling fermentation and covalent exchange It is made by joint embedding technology, and the specific preparation method is as follows:

[0039] (1) Activation and cultivation of strains: Inoculate cryopreserved Lactobacillus plantarum SCI-01 and Lactobacillus plantarum SCI-02 in MRS liquid medium respectively, culture them at 37°C for 20 hours, and subculture them for 3 times to obtain activation Strains;

[0040] The composition of MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80...

Embodiment 2

[0049] Embodiment 2 A kind of probiotic liquid preparation and preparation method thereof

[0050] A compound lactic acid bacteria micro-ecological liquid preparation, the micro-ecological liquid preparation is composed of a plant Lactobacillus (Lactobacillus.plantarum) SCI-01 and a plant Lactobacillus (Lactobacillus.plantarum) SCI-02 through symbiotic coupling fermentation and covalent exchange It is made by joint embedding technology, and the specific preparation method is as follows:

[0051] (1) Activation and cultivation of strains: inoculate cryopreserved Lactobacillus plantarum SCI-01 and Lactobacillus plantarum SCI-02 in MRS liquid medium respectively, culture at 37°C for 18 hours, and subculture twice in this way to obtain activation Strains;

[0052] The composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80, 0.2g ...

Embodiment 3

[0062] Embodiment 3 A kind of probiotic liquid preparation and preparation method thereof

[0063] A compound lactic acid bacteria micro-ecological liquid preparation, the micro-ecological liquid preparation is composed of a plant Lactobacillus (Lactobacillus.plantarum) SCI-01 and a plant Lactobacillus (Lactobacillus.plantarum) SCI-02 through symbiotic coupling fermentation and covalent exchange It is made by joint embedding technology, and the specific preparation method is as follows:

[0064] (1) Activation and cultivation of strains: inoculate cryopreserved Lactobacillus plantarum SCI-01 and Lactobacillus plantarum SCI-02 in MRS liquid medium respectively, culture at 37°C for 22 hours, and subculture three times in this way to obtain activation Strains;

[0065] The composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80,...

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Abstract

The invention belongs to the field of feed probiotics preparation, and particularly relates to a compound lactic acid bacterium micro-ecology liquid preparation for healthy dairy cow breeding. The micro-biology liquid preparation is prepared from Lactobacillus plantarum SCI-01 and Lactobacillus plantarum SCI-02 through symbiotic coupling fermentation and covalent cross-linking embedding technology. The compound lactic acid bacterium micro-ecology liquid preparation can be well colonized on a gastrointestinal mucous membrane after being fed to dairy cows, pathogenic bacterium breeding is restrained, the intestinal effective microbial community is improved, the organism immunity is enhanced, the number of somatic cells can be effectively decreased, the recessive mastitis morbidity is decreased, and the milk yield is increased.

Description

Technical field: [0001] The invention belongs to the field of microecological preparations for feeding, and in particular relates to a lactic acid bacteria compound microecological liquid preparation which can be used for healthy breeding of dairy cows. Background technique: [0002] As early as the 1970s, some developed countries in Europe and the United States successively developed new probiotics, hoping to replace antibiotics to a certain extent, and successively restricted or banned the use of antibiotics as feed additives. In recent years, with the country's vigorous advocacy of healthy breeding of dairy cows, my country's lactic acid bacteria microecological preparations have also been widely used in my country's feed industry. [0003] The invention patent with application number 201310465699.4 discloses a traditional Chinese medicine microecological preparation and its preparation method. Its ingredients include: probiotics, traditional Chinese medicine composition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/18A23K10/18A23K50/10C12R1/25
CPCC12N1/20C12N11/10C12N11/18
Inventor 包维臣焦丽英刘现伟
Owner 金华银河生物科技有限公司
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