Method for induced differentiation of adipose tissue-derived stromal cells into liver-like cells
A kind of mesenchymal stem cells and cell technology, applied in the field of induction and differentiation of adipose-derived mesenchymal stem cells into liver-like cells, can solve the problems of different ratio combinations of cytokines, influence on induction efficiency, cross-contamination, etc., and achieve high cell proliferation activity and high induction efficiency , The effect of easy material collection
Inactive Publication Date: 2018-11-16
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
View PDF15 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, it is difficult to obtain autologous liver tissue for induction with tissue homogenate, and the homogenate of allogeneic liver tissue may even bring about cross-contamination, which limits the later clinical application; and the transfected transcription factors may cause changes in the cell genome and make cells cancerous. Potential safety hazards are also limited in clinical applications; mesenchymal stem cells can also be induced into liver-like cells by adding specific cytokines, but the ratio of cytokines is different, and the amount of various cytokines used will directly affect the induction efficiency , and at this stage, the function of liver-like cells induced by specific cytokines is limited, the yield of target cells is low, and there are still serious deficiencies
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0028] The technical solutions of the present invention will be described in further detail below in conjunction with specific examples, but the present invention is not limited to the following examples.
[0029] To induce the differentiation of adipose-derived mesenchymal stem cells into liver-like cells, the steps are as follows:
[0030] 1. 30ml of adipose tissue comes from the abdomen of a healthy adult donor who underwent liposuction to lose weight, and signed an informed consent;
[0031] 2. After being collected in the surgical operating room of the plastic surgery hospital, it is sealed in a transport bottle for refrigerated transportation (4-8°C). 50ml of tissue protection solution needs to be added to the transport bottle. Prepared with 5μg / ml amphotericin B to ensure no bacterial and fungal contamination during transportation;
[0032] 3. After the adipose tissue arrives in the laboratory, clean the tissue cleaning solution (the tissue cleaning solution is prepare...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a method for induced differentiation of adipose tissue-derived stromal cells into liver-like cells. The method comprises the following steps: washing and cleaning adipose tissue, and adding isovolumetric tissue digestive enzyme for digestion; after completion of digestion, adding a trypsin solution for digestion; after completion of digestion, carrying out centrifuging so asto remove upper-layer grease and a digestive fluid, wherein precipitates are cells of the stromal vascular fraction of the adipose tissue; resuspending the cells of the stromal vascular fraction withphysiological saline, carrying out sieving so as to remove large tissues, carrying out washing, carrying out centrifuging so as to remove a supernatant, and after washing is completed, subjecting stromal vascular cells to inoculated culture so as to obtain the adipose tissue-derived stromal cells; selecting P3-generation adipose tissue-derived stromal cells for digestion so as to obtain a single-cell suspension, and carrying out inoculated culture with a culture medium overnight; removing a serum-free proliferation culture medium, and adding a first induction culture solution for induced culture; and replacing the first induction culture solution with a second induction culture solution for induction so as to obtain differentiated liver-like cells. The method provided by the invention canobtain functional liver cells by induction.
Description
technical field [0001] The invention belongs to the field of stem cells and regenerative medicine, and in particular relates to a method for inducing the differentiation of adipose-derived mesenchymal stem cells into liver-like cells. Background technique [0002] The liver is the largest organ in the human viscera. It mainly performs metabolic functions, and plays a role in the body in deoxidizing, storing glycogen, synthesizing secretory proteins, and secreting bile to assist in fat digestion and so on. It plays an important role in human health, and liver failure can even be life-threatening. [0003] With the development of society, liver damage, including acute liver failure, various chronic liver diseases, and even liver cancer threaten the health of more and more people. Liver orthotopic transplantation is the best way to treat end-stage liver disease, but liver transplantation faces many problems, such as limited donors and severe immune rejection. The development ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/067C12N2506/1384C12N2501/39C12N2501/237C12N2501/18C12N2501/11C12N2501/115
Inventor 朱建斌江嘉豪何美第陈炽峰康斯敏陈碧莹蔡祥胜王明珠王进辉
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
Features
- R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
Why Patsnap Eureka
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com