Preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome

[0027] Lutein, cholesterol and dipalmitoylphosphatidylcholine were dissolved in chloroform-methanol solution with a volume ratio of 2:1 to obtain a solution containing 0.1% lutein lipid. The organic solvent was removed by rotary evaporation, so that the compound formed a uniform film at the bottom of the round-bottomed flask, and the vacuum degree was increased to 0.1 MPa for 30 min. Add a phosphate solution to the composite film for hydration, the hydration temperature is 60°C, and the time is 120min; under the condition of stirring in an ice-water bath, ultrasonic, the ultrasonic power is 800W, the pulse is 5s / 5s, after the treatment time is 20min, continue to stir at 30°C for 3h , the stirring speed is 1000r / min; the dispersion liquid is centrifuged at 10000r / min for 10min to remove unembedded lutein. α-Glucosyl hesperidin solution (0.5%) was added dropwise to the dispersion, and stirred overnight at room temperature. The resulting dispersion was squeezed 10 times through ...

[0031] Lutein, cholesterol and dipalmitoylphosphatidylcholine were dissolved in a chloroform-methanol solution with a volume ratio of 2:1 to obtain a 2% lutein lipid solution. The organic solvent was removed by rotary evaporation, so that the compound formed a uniform film at the bottom of the round-bottomed flask, and the vacuum degree was increased to 0.1 MPa for 30 min. Add a phosphate solution to the composite film for hydration, the hydration temperature is 55°C, and the time is 100min; ultrasonication is performed under the condition of stirring in an ice-water bath, the ultrasonic power is 800W, the pulse is 5s / 5s, and after the treatment time is 30min, the stirring is continued at 40°C for 3h , the stirring speed is 1000r / min; the dispersion liquid is centrifuged at 10000r / min for 10min to remove unembedded lutein. α-Glucosyl hesperidin solution (10%) was added dropwise to the dispersion, and stirred overnight at room temperature. The resulting dispersion was squeezed...