Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method

[0004] Specific embodiment one: this embodiment mode is realized through the following steps: one, the cell concentration is 5 * 10 9 A / L of hydrogen-producing bacteria liquid, inoculated into the strain nutrient solution at a volume ratio of 1%, placed in a constant temperature air bath oscillator, the rotation speed of the oscillator was 120 rpm, and the temperature was controlled at 34-36 ℃, cultivated for 20-30 hours, and obtained the strain sample of the first-level expanded culture; 2. Inoculated the strain cell suspension of the first-level expanded culture into the fermenter containing the sterilized liquid nutrient solution with a volume ratio of 1-3% Carry out secondary expansion culture in the fermentation tank, the temperature inside the fermenter is controlled at 34-36 °C, the stirring speed is 120 rpm, and the hydrogen-producing bacteria are cultivated in the fermenter for 40-50 hours before continuous cultivation and continuous feeding; three. , at two effective...

[0005] Specific embodiment 2: In step 1 of this embodiment, the temperature is controlled at 35°C. Other methods and steps are the same as those in the first embodiment.

[0006] Specific embodiment three: In step one of this embodiment, the incubation time is 24 hours. Other methods and steps are the same as those in the first embodiment.