85 results about "Arctiin" patented technology

The invention relates to a method of preparing antivirus and antitumor natural medicine arctigenin, and its characteristic: crushing the Arctium lappaL., using organic solvent to degrease, and obtaining a crude arctiin, then using snailase to make fermentation and enzymolysis the on the crude arctiin in an enzyme reaction system, and finally terminating the enzymolysis reaction by alcohol-containing water-soluble organic solvent and extracting enzymolysis products, then separating the enzymolysis products by using silica gel column chromatography and solvent recrystallization, purifying and obtaining the pure arctigenin; the yield of the method is increased by at last over 20 times as compared with that of separating natural-existing arctigenin in plants like Arctium lappaL.

The invention provides a preparation method of arctium fruit extract for decreasing blood sugar, wherein the extract comprises Arctiin, Matairesinol, Lappaol A, Lappaol C, Lappaol Fú¼LappaolHú¼Arctignan Eú¼beta-sitosterol and daucosterol.

Present Invention relates to a skin whitening cosmetic composition comprising arctiin, arctigenin or a mixture thereof as an active ingredient. The disclosed composition can control or inhibit the synthesis of melanin by acting on a melanin synthesis route of α-MSH secreted by UV, and thus show an excellent whitening effect compared to a conventional skin whitening cosmetic. Also, the composition has high formulation stability and low skin irritation.

The invention discloses a preparation method of high-purity arctigenin. The preparation method of the high-purity arctigenin does not need a heating return flow method to extract arctigenin constituents, does not need an enzymolysis method and a column chromatography, and directly uses ethyl acetate diacolation to extract arctiin in burdock, the potential of hydrogen (PH) value of a diacolation concentrated solution is adjusted to be 3.0-4.0, the arctiin is directly hydrolyzed to become an arctigenin crude product, and an opposite phase preparative liquid chromatography optimized by a large number of experiments is adopted to obtain the high-purity arctigenin. The preparation method of the high-purity arctigenin is reasonable and in the entire preparation process design, high in preparation efficiency, strong in operability, and suitable for industrialized mass production.

The invention relates to a preparation method of arctiin, which is simple to operate and has the advantages of low pollution and low energy consumption. The method comprises the following steps: taking dry ripe fruits of burdock,, adding into a CO2 supercritical extractor, extracting by using ethanol as an entrainer to obtain an extract, recovering the solvent while depressurizing, carrying out multistage countercurrent extraction by using an ethyl acetate-water (10:2) mixed solvent, merging the extract liquids, recovering the solvent while depressurizing, concentrating the solvent, adding the solvent to macroporous adsorbent resin columns to be adsorbed, eluting with ethanol, collecting the eluent, recovering ethanol while depressurizing, concentrating, adding acetone to crystallize, separating the crystals, washing and drying to obtain the arctiin. The method for preparing arctiin has the advantage of high product purity, and can easily implement industrial scale-up production.

The invention provides a method for preparing arctigenin, which includes the steps: arctiin is used as a substrate, Beta-glucosidase enzyme liquid produced by the fermentation of aspergillus niger CGMCC No. 2594 is used as a catalyzer, the subsrate and the catalyzer are transformed in a shaker at the temperature of 20 DEG C to 50 DEG C and at the speed of 100r / min to 200r / min for 40h to 64h; and the transformed solution is separated and purified to obtain the arctigenin. The method has the advantages of: 1. mild reaction conditions and being environmental-friendly; 2. simple preparation process of a biocatalyst, namely the Beta-glucosidase enzyme liquid, needing no complex zymoprotein separation and purification as well as low cost; 3. simple operation and relatively high biological transformation efficiency; and 4. easy amplification of the biological transformation process and easy realization of large scale industrialized production. By utilizing the method for producing, the molar yield of the arctigenin can reach 94.7 percent and the purity of the product can reach 99.7 percent.

The invention relates to a composition containing 50 to 99 parts by weight of arctiin with the purity of more than 90 percent or 1 to 50 parts by weight of arctigenin and a preparation method thereof, and the functions of the composition are the treatment and the prevention of diabetic retinopathy. In the invention, by extracting the arctiin and the arctigenin, the pharmacological, toxicological and clinical trial results show that the composition of arctiin or arctigenin of the invention has the advantages of strong treatment effect and small toxicity and side effects in the treatment of the diabetic retinopathy.

The present invention provides the application of arctiin or arctigenin in preparing medicine for preventing and treating diabetes and its complication. The present invention also provides the application of arctiin or arctigenin in preparing medicine composition for preventing and treating diabetes and its complication, and the medicine composition may be administrated through oral taking, injection or local application.

The invention discloses a method for preparing arctiin. The method mainly comprises the following steps of: extracting a burdock medicinal material with water; filtering the extracting solution, and directly introducing into a macroporous resin column for adsorbing and enriching; eluting gradually with water and an ethanol solution in sequence; collecting the needed eluent, concentrating and filtering; treating with a decoloring resin column to obtain crude arctiin; and crystallizing with a water-containing mixed solvent to obtain a finished product of which the purity is more than 90 percent. The method has the advantages of simple process, continuous operation, convenience in automation, convenience for recycling solvents, low energy consumption, high product purity and easiness for realizing industrial amplification.

Disclosed is a method for preventing and / or soothing dry or sensitive skin against external stresses, comprising contacting the skin with a composition including an agent that decreases the release of granulocyte-macrophage colony-stimulating factor (GMCSF) from keratinocytes in the skin, wherein under conditions of external stress, dry or sensitive skin is soothed and / or prevented. Agents that decrease the release of GMCSF include silybin, piperine, tetrahydropiperine, nortrachelogenine, rhapontin, arctiin, artigenin and mixtures thereof. Also disclosed is a method of identifying an agent that decreases the level of GMCSF released from keratinocytes.

The invention discloses an application of heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F to transforming arctiin into arctigenin. Amino acid sequences of the heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F are indicated in SEQ: ID NO.1, ID NO.2 and ID NO.3. The arctiin is hydrolyzed to prepare the arctigenin by the aid of heat-resisting beta-glucosidase and the mutants thereof, and as compared with wild-type enzyme, the heat-resisting beta-glucosidase has the advantages of high transformation efficiency, fewer reaction by-products, high yield and purity, easiness in separation and purification, less chemical pollution and the like.

The invention relates to sugar-free anemopyretic cold granules and a preparation method thereof. The granules mainly comprise major materials and auxiliary materials, wherein the major materials comprise isatis root, weeping forsythia, mint, schizonepeta spike, mulberry leaf, rehmannia root, great burdock achene, chrysanthemum, bitter apricot seed, mulberry twig and medicated leaven; and the auxiliary materials mainly comprise stevioside, aspartame, sodium starch glycolate and an appropriate amount of malto dextrin. The preparation method comprises the following steps of: extracting volatile oil from mint and schizonepeta spike for later use; dosing decoction dregs left after the extraction of volatile oil and other major materials in a specified sequence; decocting three times; filtering; concentrating the filtrate at reduced pressure to obtain clear paste of which the relative density is 1.05-1.15 (60 DEG C); performing spray drying to obtain extract powder; adding the screened auxiliary materials into the extract powder; putting the mixture into a dry type granulator to obtain granules of 10-30 meshes; spraying the extracted volatile oil; mixing uniformly; and packaging. The granules prepared with the method have the advantages of no containing of sugar cane, small using quantities of auxiliary materials, greatly lowered dosing amount compared with the original granules, low production cost and high content of arctiin serving as an active ingredient after process improvement.

A process for preparing arctiin and arctigenin used to prepare meticines features that the liquid extract of arctium fruit passes through a polyamid column for separating and purifying.

Present Invention relates to a skin whitening cosmetic composition comprising arctiin, arctigenin or a mixture thereof as an active ingredient. The disclosed composition can control or inhibit the synthesis of melanin by acting on a melanin synthesis route of alpha-MSH secreted by UV, and thus show an excellent whitening effect compared to a conventional skin whitening cosmetic. Also, the composition has high formulation stability and low skin irritation.

The invention provides a method for separating burdock oil, arctiin, arctigenin, lappaol E and lappaol H from burdock. The method for separating the burdock oil, arctiin, arctigenin, lappaol E and lappaol H from the burdock comprises the following steps: firstly degreasing burdock through CO2 supercritical fluid extraction, separating to obtain the burdock oil and degreased burdock powder, carrying out water-alcohol extraction and concentration on the degreased burdock powder to obtain extract, carrying out CH2Cl2 extraction on the extract, loading the extract onto a silica gel column to be subjected to chromatography, carrying out dichloromethane-methanol gradient elution, carrying out thin-layer chromatography, and purifying, so as to obtain the arctiin, lappaol E, arctigenin and lappaol H. The method provided by the invention has the advantages that multiple products can be separated by adopting one continuous route only, raw materials are fully utilized, resources are saved, a degreasing process does not pollute the environment, the obtained burdock oil has high nutritive value, and the arctiin, arctigenin, lappaol E and lappaol H are high in purity and can be independently or mixed with other substances to be applied to the medicinal and edible field as raw materials.

The invention relates to a method for preparing arctiin, which has the advantages of simple and convenient operation, light pollution and low equipment input. The method comprises the following process steps: taking crude powder of great burdock achene, adding petroleum ether of which the volume is 3 to 8 times weight of the crude powder, and heating and refluxing for 1 to 3 hours; discarding the petroleum ether, volatilizing dreg of decoction to dryness, adding 60 to 90 percent ethanol of which the volume is 3 to 10 time weight of the dreg of decoction, refluxing for 1 to 3 times, and refluxing for 1 to 3 hours each time; merging liquor, filtering, recovering the ethanol under reduced pressure, concentrating the liquor, adding water with equal volume, uniformly stirring, and filtering; taking filtrate, adding the filtrate to a macroporous adsorption column for adsorption, eluting with 10 to 50 percent ethanol, and collecting elution of which the volume is 3 to 8 times volume of the column; and recovering the ethanol under reduced pressure, concentrating the elution, and adding dichloromethane, ethyl acetate and methanol (4:4:1) for crystallization. The arctiin prepared by the method has high purity and is easy for industrial amplification.

The invention discloses a detection method of Yinqiao powder. The detection method can simultaneously determine the content of chlorogenic acid, forsythin, forsythiaside A and arctiin. The detection method has the advantages of high efficiency, fastness, high accuracy, high sensitivity, good repeatability, reliable result, effective control of the quality of the above product, and guaranteeing of clinic medicine effects of the above medicine.

The invention relates to a kind of Chinese traditional medicine that can decrease the saccharide content and tonify kidney with the total lignanoid extracts from arctii. The content of the total lignanoid extracts from the arctii is 50-89%, the content of the main active ingredient (arctiin) is more than 30%. Using the valid total lignanoid as the material, the producers can prepare different per oral preparations. The advantages of the invention includes: 1. the invention can decrease the blood sugar substantially, it can prevent the damage to kidney because of hyperglycemia, it can even treat the early kidney damage; 2. the preparing method is safe and simple.

A construction method of an HPLC specific chromatogram of a wind-heat common cold granule includes the following steps: (1) preparation of a reference substance solution: precisely weighing a proper amount of an arctiin reference substance, adding 30-70% methanol to produce an arctiin methanol solution with concentration of the arctiin being 60-80 [mu]g / ml; (2) preparation of a test sample solution: finely grinding the wind-heat common cold granule, and precisely weighing about 1-3 g of the granule; (3) setting of chromatographic condition: employing a ZORBAX SB-C18 chromatographic column, employing a mixed solution of acetonitrile-0.1% phosphoric acid solution with ratio being 5-45:95-55 as a mobile phase, setting flow velocity to be 0.5-1.5 ml / min, preparing a DAD detector, wherein detection wavelength is set as 205-285 nm, setting column temperature to be 25-45 DEG C and setting number of theoretical plates to be not less than 10000; and (4) measurement: precisely sucking 5-20 [mu]l of each of the reference substance solution and the test sample solution, injecting the solutions into the liquid chromatographic instrument, and performing HPLC detection to obtain the HPLC specific chromatogram of the wind-heat common cold granule. The method can effectively monitor the quality of the wind-heat common cold granule in different batches. The method has high precision and good repeatability, and is used for comprehensively monitoring the quality of the product.

The invention discloses a method for simultaneously measuring contents of arctiin, glycyrrhizic acid and pulegone in traditional Chinese medicine. The method comprises the steps that firstly, arctigenin, glycyrrhizinate and pulegone standard substances and a sample containing fructus arctii, licorice root and schizonepeta spikes (or schizonepeta) are taken respectively to be dissolved in a methanol aqueous solution, and a standard solution and a sample solution are prepared respectively; secondly, the standard solution and the sample solution which are identical in volume are taken to be injected into an efficient liquid chromatograph, then the peak area of the standard solution and the test solution is measured, and the contents of arctiin, glycyrrhizic acid and pulegone in the sample are obtained. The quick and accurate method is provided for measuring the contents of arctiin, glycyrrhizic acid and pulegone in traditional Chinese medicine containing fructus arctii, licorice root and schizonepeta spikes (or schizonepeta) and in extracts or preparations of the traditional Chinese medicine.

The invention belongs to the field of natural organic chemistry, and particularly relates to a method for enriching and purifying arctiin in burdock by using macroporous adsorbent resin. By using macroporous adsorption technology to separate and purify arctiin, the invention has the characteristics of good adsorption selectivity for arctiin, high adsorption and desorption speed, high adsorption capacity, abundant material sources, low production cost, obvious separation effect and high extraction purity, can be used for extracting arctiin conveniently and quickly, can be used to obtain a semi-finished product of which the content of arctiin is higher than 35% and a final product of which the content of arctiin is higher than 90%, and can overcome the defects of low extraction yield and low extraction purity in the conventional method. The macroporous adsorbent resin, which has the advantages of stable physicochemical properties, large surface area, high exchange speed, high mechanical strength, high antipollution capability and high thermal stability, is used for selectively adsorbing arctiin from the solution in a physical adsorption mode, and thus, the invention has the advantages of high adsorption and desorption speed, and high adsorption capacity.

An extract of eucommia ulmoides and its preparation method and application belong to the technical field of traditional Chinese medicine. The Eucommia ulmoides extract contains olivine 9-glucoside, olivine 4'-glucoside and arctiin, and their mass percentages are respectively 3.10-3.70%, 1.40-1.70%, and 9.50-10.50%. It is prepared by pulverizing Eucommia ulmoides, extracting, concentrating, centrifuging, standing, and separating with macroporous adsorption resin. Has a significant anti-inflammatory effect.

The invention discloses a guinea fowl feeding healthcare powder which is prepared from the follow raw materials in parts by weight: 0.8-1.2 parts of polygonatum, 0.5-0.7 part of angelica, 0.2-0.4 part of astragalus, 0.2-0.4 of isatis root, 0.2-0.4 part of medicated leaven, 0.2-0.4 part of arctiin complanatus, 0.2-0.4 part of semen astragali complanati, 0.2-0.4 part of bupleurum, 0.2-0.4 part of cassia twig, 0.2-0.4 part of cimicifugae foetidae, 0.2-0.4 part of pueraria, 0.2-0.4 part of epimedium, 0.2-0.4 part of orange peel, 0.2-0.4 part of garlic powder, 1-2 parts of carrot, 1-2 parts of hawthorn, 1-2 parts of silkworm chrysalis, 1-2 parts of black soybean meal, 1-2 parts of sweet potato leaves and a proper amount of honey. The raw materials are made into an extract by adopting the conventional method, and then the extract is dried and ground into powder. The guinea fowl feeding healthcare powder can be fed to the guinea fowls to improve the disease resistance of the guinea fowls effectively. The manure of the guinea fowls fed with the guinea fowl feeding healthcare powder is not smelly, and the guinea fowl feeding healthcare powder is particularly beneficial to purifying the environment of the large-scale guinea fowl farm. If the guinea fowl feeding healthcare powder is fed to the young guinea fowls, the meat of the guinea fowls in the growing stage has a unique flavor, and even the flavor still remains on the killed guinea fowls.

The invention discloses an extraction, separation and purification method of arctiin. The method comprises the following steps: a) ethanol extraction, namely grinding arctium lappa, adding an ethanol solution which is preferably 5-10 times by weight, carrying out reflux extraction for three hours for two to three times and mixing the extracting solutions; b) activated carbon decoloration, namely adding activated carbon which accounts for 0.5% of the extracting solution by volume, carrying out reflux decoloration and carrying out filtration, thus obtaining a decoloration solution; c) concentration, namely recycling the decoloration solution at reduced pressure and enabling about 20% by volume of ethanol solution to be remained; d) impurity removal, namely adding a mixed solution of petroleum ether and ethyl acetate, stirring the solution and extracting the impurities and repeating the step four-six times; e) arctiin extraction, namely adding a dichloromethane to extract product and repeating the step five-eight times; f) concentration and crystallization, namely mixing dichloromethane extracting solutions containing products, concentrating the extracting solution, adding methanol to dissolve the concentrate and adding 20% by volume of isopropyl ether for crystallization, wherein the concentration of the ratio of ethanol to water is 70-95%; the ratio of petroleum ether to ethyl acetate is (1:1)-(1:3). The method has the beneficial effects that the method is convenient to operate, is low in production cost and easily achieves industrial production.

The invention discloses a method for preparing arctiin, which comprises the following steps of: crushing burdock and extracting the crushed burdock by using water or alcoholic solution; allowing the extract to directly enter an absorption column to perform adsorption and enrichment, eluting the product with the water and the alcoholic solution in turn, and performing concentration under reduced pressure to obtain arctiin fraction; and performing countercurrent chromatographic separation on the arctiin fraction by using a two-phase system formed by aqueous solution consisting of a single organic solvent and salt to obtain salt arctiin fraction, and performing adsorption to remove the salt by using macroporous adsorption resin or ion exchange resin. The method has the advantages of simple process, continuous operation, contribution to automation and recovery and recycling of the solvent, and suitability for industrial mass production.

The present invention discloses the application of arctiin as one kind of Chinese medicine extract in preparing medicine for treating rhinitis and nasosinusitis. The medicine for treating rhinitis and nasosinusitis contains arctiin not less than 5 wt% and may contains other Chinese medicine components. The medicine may be prepared into capsule, granule, bolus, tablet, decoction or other oral preparation form. The present invention has the functions of clearing away heat and toxic material, dispelling wind, relieving stasis, eliminating inflammation and drawing out pus, and can treat rhinitis and nasosinusitis in high curative effect and no toxic side effect.

The invention discloses a detection method of a traditional Chinese medicine composition capable of invigorating brain and tonifying kidney, and belongs to the technical field of traditional Chinese medicine quality detection. According to the detection method, high performance liquid chromatography is adopted, under the same chromatographic conditions, paeoniflorin is taken as a reference, and characteristic qualitative analysis on chlorogenic acid, spinosin, forsythin, and arctiin in samples is carried out. The detection method is simple and rapid, and is high in sensitivity and stability; acomposition characteristic spectrum is obtained; and comprehensive detection of all contents of the composition is realized.

The invention provides a processing extraction method of siraitia grosvenorii fruits. The extraction method comprises the steps that the siraitia grosvenorii fruits are quickly washed, cut into segments, dried, taken out, soaked for 0.5 h by adding water of which the weight is 10 times the siraitia grosvenorii fruits, decocted for 1 h and then filtered; the medicine dregs are decocted for 1 h by adding water of which the weight is 8 times the medicinal materials, and medicinal liquid is filtered; first-time decoction liquid and second-time decoction liquid are mixed, and the mixed liquid is concentrated until the relative density is 1.05 at 60 DEG C; ethyl alcohol is added into the concentrated liquid to enable the volume percent of the alcohol content to reach 75%, stirring, standing and filtering are conducted, the filtrate is concentrated until the relative density is 1.25 at 65 DEG C, ethyl alcohol is recycled, and concentrated liquid is obtained; refining is conducted through silica gel, the selected silica gel is contained in resin columns in advance, impurities in the concentrated liquid are removed through reverse phase silica gel columns after the concentrated liquid is diluted by purified water, and all effluent is collected; the effluent continues to be concentrated, the concentrated liquid is dried and granulated to obtain siraitia grosvenorii fruit granules, and high-content mogroside V is prepared and is compatible with arctiin and catechin to prepare the siraitia grosvenorii fruit composition.

This invention relates to a method for preparing 4, 4'-dihydroxy enterolactone from arctiin. The method comprises: utilizing arctiin as the precursor compound, and converting arctiin into 4, 4'-dihydroxy enterolactone by intestinal bacteria in an anaerobic condition. 4, 4'-dihydroxy enterolactone is lignin compound, and can be converted into phytoestrogen (enterolactone) in human intestinal canals. The method simplifies the synthesis process of 4, 4'-dihydroxy enterolactone, widens the pharmaceutical applications of arctiin, and provides a new healthcare food for preventing and treating climacteric syndrome, osteoporosis, hyperlipemia, breast cancer and endometritis.

The present invention relates to a pharmaceutical composition for the prevention and treatment of inflammatory diseases comprising The present invention relates to a pharmaceutical composition for the prevention and treatment of inflammatory diseases comprising Trachelospermi caulis extract as an active ingredient. More particularly, the pharmaceutical composition of the present invention is characterized in that it is standardized and formulated so that the arctiin is contained in the Trachelospermi caulis extract within a predetermined range to exhibit excellent inhibitory effects on pains, acute inflammation, acute edema, production of iNOS, and TNF-Yá, activation of the enzymes of MAP kinases and NFYêB, thus effective in the prevention and treatment of inflammatory diseases such as arthritis.