The invention discloses an establishment method and a fry culture method of a 
heterologous hybrid culter strain. The establishment method comprises the steps that 
erythroculter ilishaeformis in the 
Changjiang river system serves as a female parent, 'Pujiang No.1' 
megalobrama amblycephala serves as a male parent, artificial induced spawning, fertilization, 
hatching, fry culture and screening are carried out, and hermaphroditic fertile diploid 
hybrid culter F1 is obtained; the 
hybrid culter F1 and the male parent 
erythroculter ilishaeformis in the 
Changjiang river system are subjected to backcross, 
hatching and breeding are carried out, diploid hybrid culter F2 is obtained, and establishment of the 
heterologous hybrid culter strain is completed; the diploid hybrid culter F2 is subjected to self-cross, screening is carried out, hybrid culter F3 is obtained, hybrid culter F4 and hybrid culter F5 are obtained in the same way, and the stable 
heterologous hybrid culter strain is obtained. Compared with the prior art, by means of the establishment method of the heterologous hybrid culter strain, the novel hybrid fish, namely heterologous hybrid culter, which has the hybrid advantages of a high growing speed, tightly-closed scales, stable hypoxia resistance inheritance, hermaphroditic 
fertility and the like can be cultured, and great significance is achieved on the aspects of fish genetic breeding and 
biological evolution.