43 results about "Cellulomonas" patented technology

The invention relates to a cellulomonas fimi DA 8 bacterial strain as well as an acquiring method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has flax degumming activity. The cellulomonas fimi bacterial strain screened by the method has the advantages of large enzyme yield, complete enzyme system, no toxicity, rough culturing condition and the like; after used for flax degumming, the composite degumming enzyme solution not only greatly shortens a degumming period, but also reduces the cost, the fiber splitting degree is improved, and the degumming efficiency reaches 95%. The degumming method requires basically the same equipment required in the traditional warm water degumming method so that the method is especially suitable for improving a process on the basis of the traditional equipment in old enterprises; the cellulomonas fimi bacterial strain screened with the method not only can be used for flax raw-stem degumming, roving scouring, flax fabric biological pretreatment, but also can be used for bast fiber degumming and biological pretreatment of bast fiber plants, such as ramie, jute, hemp and the like.

The invention relates to a microbial inoculum for fermenting and producing organic fertilizers, comprising cellulomonas, bacillus, lactobacillus, methagen, thiobacillus, streptomyces, thermoactinomyces, thermomonospora, azotobacter, nitrosomonas, nitrobacter, rhizobium, aspergillus, yeast, penicillium, humicola, rhizopus and mycorrhizal fungi. In addition, the invention also relates to organic fertilizers fermented and produced by using the microbial inoculum and a method for fermentation and production.

The invention relates to an amylosucrase and a method for using same to produce Alpha-arbutin by transformation, which belong to the field of food biotechnology. The invention relates to a recombinant amylosucrase cellulomonas carboniz T26 (registry number: KGM11272.1), the amylosucrase is then added into 1 to 30 percent by mass concentration of sucrose-hydroquinone solution (molar ratio: 1:1) to catalytically synthesize Alpha-arbutin, transformation takes place for 3 to 4 hours, the transformation rate is 60 percent or more, antioxidant Vc is not needed, and the amount of donor demanded is small. Alpha-arbutin produced by the invention is a highly effectively skin-whitening product which has a great market potential, and can be widely applied in industries such as food, cosmetics and pharmacy. The amylosucrase and the method can efficiently produce Alpha-arbutin, and are suitable for mass production.

The invention discloses a method for producing a feed from straw through steam explosion. The method comprises the following steps of firstly, treating the straw with a rubbing and crushing machine, and then enabling the treated straw to enter a steam explosion machine, wherein the steam explosion temperature is 110-150 DEG C; and enabling the straw after steam explosion to be subjected to water supplement until the weight of the water is 60-75% of that of the straw, adding ammonium sulphate which is 3-9% of the weight of the straw as an additional nitrogen source, performing inoculating witha mixture of one or more of bacillus subtilis, bacillus licheniformis, white rot fungi, cellulomonas liazotes, candida utilis, lactobacillus amylovorus, lactobacillus acidophilus and microzymes for fermentation, wherein the quantity of strains for fermentation is 1-100 billions of strains / grams (dry basis), compacting and sealing the inoculated steam explosion straw, and performing anaerobic fermentation under 10-35 DEG C for 7-50 days. After fermentation, through detection, the content of crude protein can achieve 20% or above. According to the method disclosed by the invention, the straw isused as the raw material, through steam explosion, lignin of the straw and the crystal and winding of cellulose are destroyed, and further fermentation is facilitated. The composite fermentation stains are used for fermentation, in the fermentation process, mycelium protein and volatile fatty acids are generated continuously, and the nutrient and the quality of the straw feed are further improved.

The invention discloses a preparation method of baked type probiotic fermented jam. The preparation method comprises the following processes: preparation of fermentation agents: culturing a strain A,namely fermented cellulomonas, a strain B, namely aspergillus niger, a strain C, namely bacillus coagulans, a strain D, namely lactobacillus plantarum, and a strain E, namely bacillus subtilis, so asto prepare the fermentation agents of the strains A, B, C, D and E; preparation of compound juice: compounding bananas with other fruits and vegetables, and performing juicing to prepare the compoundjuice; preparation of seed liquid: respectively adding the compound juice to the fermentation agents of the strains A, B, C, D and E to prepare the seed liquid of the strains A, B, C, D and E; preparation of raw materials: taking the bananas as a carbon source, and compounding the bananas with other fruits and vegetables; pulping: pulping the raw materials and sterile water together to obtain compound pulp; and fermentation of compound strains: mixing the seed liquid of the strains A, B, C, D and E into the compound pulp, and performing fermentation to obtain the fermented jam. The preparationmethod has the advantages that sugar does not need to be added, heat-resistant type probiotic fermented jam suitable for baking can be prepared, and the baked type probiotic fermented jam combines the advantages of probiotic fermented jam and baked jam.

The invention provides an Ageratina adenophora organic fertilizer solid microbial inoculant which is formed by mixing a tertiary strain of Pseudomonas fluorescens and a tertiary strain of Cellulomonas. The Ageratina adenophora whole plant is directly treated in situ in the open air; and thus, the method is economical and applicable, has the advantages of low cost, labor saving and wide application range, and is simple to operate and easy to popularize, thereby providing a new idea for solving the problem of Ageratina adenophora disasters.

The invention belongs to the technical field of microorganisms, and discloses a biological preparation for treating a monosodium glutamate fermentation mother liquid. The biological preparation is prepared by the following method: mixing xylose-oxidized bacillus, micrococcus luteus, coral nocardia and cellulomonas respectively according to the routine culture concentration of 1*108cuf / g at a volume ratio of (5 to 7) to (3 to 5) to (2 to 3) to (2 to 3) to obtain a liquid inoculant; stirring and mixing the liquid inoculant and a carrier according to a mass ratio of (2 to 3) to (3 to 5), drying and packaging to obtain the biological preparation. The biological preparation provided by the invention contains only four kinds of strains, and has the advantages of reasonable compatibility, symbiotic coordination, non-antagonistic performance, long service life and low sludge production.

The invention relates to a polyphosphate fungicide and application thereof, which belongs to the technical field of biology. The polyphosphate fungicide is prepared by culturing transformed bacteria and acid-forming bacteria for 4 to 6 days under the condition of 26 to 30 DEG C, selecting transformed bacteria having an effect for degrading organic phosphorus to be prepared into first bacterial suspension, selecting acid-forming bacteria having an effect for degrading the organic phosphorus to be prepared into second bacterial suspension, and then mixing the first bacterial suspension and the second bacterial suspension. The transformed bacteria is prepared from at least one of cellulomonas and bacillus, and the acid-forming bacteria is prepared from acidophil. The phosphate fungicide has efficient and stable degradation capacity for the organic phosphorus, is high in fouling resistance and has high adaptability to a polluted environment. When the polyphosphate fungicide is used for degrading the organic phosphorus, the phosphorus pollution can be effectively treated, and environmental protection and energy conservation can be realized.

The invention is about the Cellulomonas sp.nov.GJT07 which can secrete the fructosan. The strain which is separated from the soil of the orchard is same as the Cellulomonas with the morphological and the physiological-biochemical characteristic according to the ninth Bergs handbook. The strain has the good stability, so it can reach the industrial production.

The invention discloses a method for preparing a high-efficiency straw fermentation agent and belongs to the technical field of preparation of fermentation agents. Two kinds of cellulomonas used in the invention refer to a category of bacteria capable of decomposing cellulose and partial xylogen, belong to facultative anaerobes, can be released after autocytolysis and have high degradability on the cellulose under anaerobic conditions. A synergistic effect is achieved between a bacterium decomposition effect and a decomposition effect of enzymes produced in cells. The third added bacillus subtilis can produce various enzymes needed by animals, the bacterium culture medium is subjected to high-temperature or high-pressure steam treatment, sterilized and disinfected, and subjected to viablebacteria inoculation, the inoculated bacillus subtilis produces lots of spores in the reproductive process, many bacteria die during fermentation due to poor heat resistance, the bacterium death rateis reduced, the viable bacteria reach a higher concentration, and the influence on bacterial reproduction subjected to acid stress is reduced. Therefore, the aim of improving the viable bacteria countis achieved.

The invention provides a biogas fermentation promotion agent. The promotion agent is prepared from a following microorganism culture solution according to the volume part of the microorganism culturesolution, 15 to 20 parts of firmicutes, 15 to 20 parts of bacteroides, 3 to 5 parts of spirochaeta, 4 to 6 parts of thermotoga, 3 to 5 parts of deltaproteo bacteria, 3 to 5 parts of synergistetes, 3 to 5 parts of cellulomonas and 35 to 40 parts of euryarchaeota. According to the biogas fermentation promotion agent provided by the invention, selected 13 anaerobic fermentation functional strains arepreserved in strain preservation centers at home and abroad and are easy to obtain, culture and compound; according to the fermentation promotion agent provided by the invention, various microorganisms are matched reasonably and can be adaptive to fermentation raw materials with different types so that various raw materials can be rapidly degraded and converted into biogas; according to the biogas fermentation promotion agent, various microorganisms have the same or similar growth culture conditions and the promotion bacterium agent can normally grow and metabolize under the same environmentcondition and play the largest role.

The invention discloses a method for preparing turmeric saponin by virtue of microbial mixed fermentation, belonging to the technical field of biology. By virtue of the method, the problem of low yield of turmeric saponin in an existing production process is mainly solved. The method comprises the following steps: respectively culturing bacillus HG224, pectobacterium carotovorum or erwinia and cellulomonas, and mixing in an inoculating volume proportion of 1 to (0.4-1.2) to (0-1.2), so as to prepare a microbial flora; and preparing uniform turmeric slurry fromturmeric and water in a dry weight-mass ratio of (1 to 5)-(1 to 15), inoculating 10%-40% of the microbial flora into the slurry, and culturing at a temperature of 30-40 DEG C and a rotation speed of 100r / min-220r / min for 4-6 hours. By virtue of the mixed fermentation of the microbial flora, a large amount of restrained saponins arefree, so that the yield of the turmeric saponin is greatly increased, and meanwhile, the stubborn foundation is laid for the subsequent environment-friendly production of the turmeric saponin; and the method has wide industrial application prospect.

The invention provides a cellulomonas bacterial strain, a green alga hydrolysis method, a health food preparation method and prepared health food, and particularly provides the cellulomonas bacterial strain (Cellulomonas sp.YJ5); the cellulomonas bacterial strain has the ability of green algae hydrolysis; the invention also provides the green alga hydrolysis method using the cellulomonas bacterial strain, and the green alga hydrolysis method comprises cultivating of the cellulomonas bacterial strain and mixing of the cellulomonas bacterial strain with a green algae suspension liquid for the green algae hydrolysis; the invention also provides the health food preparation method using the green algae hydrolyzed by the cellulomonas bacterial strain, wherein the cellulomonas bacterial strain can promote the release of soluble proteins, chlorophyll, peptides and reducing sugar in the green algae, and can enhance the ferrous chelating ability, Trolox equivalent antioxidant capacity and reducing sugar ability.

The invention relates to the technical field of biological fermentation, and provides a medicine capable of rapidly degrading fallen leaves and a preparation method thereof. The medicine comprises thefollowing components: cellulomonas flavigena, trichoderma longibrachiatum, aspergillus niger, bacillus licheniformis, cellulomonas, arcobacter, nitrogen-fixing bacteria, bacillus subtilis, cellulose,pectinase, an additive and a culture medium. The medicine capable of rapidly degrading the fallen leaves is high in fallen leaf degrading speed and good in degrading effect; a large amount of laborsand materials can be saved in the degrading, so that the whole degrading cost is decreased; and massive application is conveniently carried out; cellulose and pectinase are added to the raw materials,so that the fallen leaf degrading speed is increased during the preparation of the culture medium, and various strains are promoted to live and reproduce on the culture medium; peat, diatom and precipitated calcium carbonate are used as the additives for dispersing various strains in the culture medium, so that the strains are promoted to reproduce and spread, and as a result, the fallen leaf degrading speed is increased.

The invention discloses a composite photosynthetic bacterial agent for sewage treatment. The composite photosynthetic bacterial agent for sewage treatment comprises the following components in parts by weight: 1-3 parts of rhodopseudomonas spheroides, 2-5 parts of rhodopseudomonas palustris, 4-6 parts of rhodopseudomonas, 3-5 parts of lactobacillus plantarum, 3-7 parts of bifidobacterium, 1-5 parts of lactobacillus acidophilus, 2-4 parts of green non-sulfur bacteria, 2-5 parts of cellulomonas, 1-3 parts of phanerochaete chrysosporium, 2-5 parts of white-rot fungi and 3-6 parts of bacillus subtilis. The composite photosynthetic bacterial agent for sewage treatment is conductive to popularization and application.

The invention discloses a composite photosynthetic bacteria agent for sewage treatment, which is composed of the following components in parts by weight: 1-3 parts of Rhodopseudomonas sphaeroides, 2-5 parts of Rhodopseudomonas swamp, and Rhodopseudomonas 4-6 parts of bacteria, 3-5 parts of Lactobacillus plantarum, 4-5 parts of phosphorus accumulating bacteria, 1-5 parts of Aspergillus oryzae, 4-6 parts of nitrous bacteria, 3-5 parts of Cellulomonas, Chrysosporium 1-3 parts of leather bacteria, 2-5 parts of white rot fungi and 3-6 parts of Bacillus subtilis. The present invention is favorable for popularization and application.

The invention discloses feed for a pregnant sow. The feed is characterized by being prepared from raw materials in parts by weight as follows: 120 parts of corn residues, 90 parts of bean dregs, 90 parts of wheat bran, 65 parts of sorghum flour, 25 parts of vegetable residues, 13 parts of Chinese yam, 13 parts of sesame, 18 parts of shiitake mushrooms, 7 parts of hair weeds, 13 parts of fructus lycii, 8 parts of fructus crataegi, 8 parts of cornus officinalis, 12 parts of Chinese dates, 6 parts of angelica sinensis, 3 parts of malic acid, 18 parts of plant oil, 2 parts of liver powder, 2 parts of calcium hydrophosphate, 1 part of yeast, 1 part of cellulomonas, 3 parts of fine sugar and a proper amount of table salt through mixing. The sow fed on the feed gains weight rapidly, does not get sick, is full of vigor, active in foraging and has glowing skin, and the weight of the sow can increase by 46% or above than that of sows fed on conventional feed. Piglets delivered by the sow fed on the feed is about 2 liang heavier than those delivered by the sows fed on conventional feed, have no abnormalities and have no dead fetus, and the feed is suitable for popularization.

The invention belongs to the technical field of agriculture and in particular relates to a compound bacterium agent for fermenting organic fertilizer. The compound bacterium agent is characterized bybeing prepared from lactobacillus, aspergillus, cellulomonas, butyrivibrio fibrisolven, cytophaga psychrotropha, pseudomonas, bacillus subtilis, trichoderma and actinomyces. The compound bacterium agent provided by the invention has the effect of improving the content of organic matters and total nutrients in the organic fertilizer and the fertilizer efficiency of the organic fertilizer is improved.

The invention relates to a combination method of a compound microbial agent for kitchen garbage degradation. The compound microbial agent comprises bacillus subtilis, streptomyces, saccharomycetes, actinomycetes, lactic acid bacteria, pseudomonas, microbacterium, rhizopus oryzae, cellulomonas and aspergillus. Through optimization and compounding, a combination with relatively high conversion efficiency is obtained. The compound microbial agent is mainly used for converting organic matters such as protein, starch, fat and cellulose in the kitchen garbage into stable products through metabolic activity of microorganisms, and the stable products can be further processed into products such as fertilizers. Herein, the research results are beneficial to harmless, reduction and resourceful treatment of the kitchen waste, and a certain theoretical basis can be provided for efficient large-scale treatment of the kitchen waste.

The invention discloses a moudly spot appearing method for raw Pu'er Fu tea. The method comprises the steps of treatment of raw Pu'er tea, wherein compressed aging raw Pu'er tea is wetted to be dispersed; softening of the raw Pu'er tea, wherein cellulomonas and bacillus cereus are added into drinking water to be mixed into a softening solution, and the softening solution is sprayed to the surfacesof raw Pu'er tea leaves; sterilization, wherein the softened raw Pu'er tea is sterilized through ultraviolet rays; preparation of a fermentation broth, wherein aspergillus pseudoglaucus is separatedfrom Fuzhuan tea and is prepared into a leaving agent; pile fermentation fermentation, wherein the fermentation broth is sprayed on the sterilized raw Pu'er tea leaves; drying, wherein the tea leavesare firstly placed under the sun for natural air-drying, then further drying is performed through a dryer; the dried tea leaves are weighed and packaged in bags. The invention relates to the technicalfield of raw Pu'er tea. The fermentation method of the raw Pu'er tea achieves the purposes of softening the tea leaves before fermentation, facilitating subsequent fermentation, accelerating the fermentation progress by adding the aspergillus pseudoglaucus during fermentation, improving the tea quality, and enhancing the functions of the human body.

The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.

The invention is about the Cellulomonas sp.nov.GJT07 which can secrete the fructosan. The strain which is separated from the soil of the orchard is same as the Cellulomonas with the morphological and the physiological-biochemical characteristic according to the ninth Bergs handbook. The strain has the good stability, so it can reach the industrial production.

The invention provides a cultivation method for increasing the yield of termitomyces albuminosus, and relates to the field of cultivation of fungi. The cultivation method comprises the following stepsof weeding of a bacteria pond, soil loosening, watering of the bacteria pond, earthing of the bacteria pond, water and fertilizer management and filed management. A nutritional agent is prepared from6-10 parts of corn flour, 11-14 parts of sugarcane stalks, 5-9 parts of a moist cellulomonas solution, 10-13 parts of termite mud, 3-5 parts of formic acid, 7-10 parts of urea and 3-11 parts of bran.The cultivation method for increasing the yield of termitomyces albuminosus has the advantages that for the found termitomyces albuminosus pond, the soil of a surface layer of the found termitomycesalbuminosus pond is processed, termitomyces albuminosus can be produced 1-2 months earlier through reasonable water and fertilizer management, termitomyces albuminosus grows more stoutly and stronglyand larger, the yield can be doubled or above every year, and the yield and quality of termitomyces albuminosus are significantly improved.

A process for the creation of ethanol from cellulosic materials using the bacteria Cellulomonas sp. and aerobic Zymomonas mobilis in the same medium under the same conditions to breakdown cellulosic materials into glucose and to ferment that glucose into ethanol and three significant byproducts, glycerol, acetic acid, and lactic acid.

The disclosure relates to (i) a gene isolated from Cellulomonas sp.JCM2471, the gene encoding a new glycerol kinase and (ii) a method for preparing the glycerol kinase by gene recombination technique, A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant vector, and a method for producing the glycerol kinase, including culturing the transformant to produce glycerol kinase, and collecting the resulting glycerol kinase.

A process for the creation of ethanol from cellulosic materials using the bacteria Cellulomonas sp. and aerobic Zymomonas mobilis in the same medium under the same conditions to breakdown cellulosic materials into glucose and to ferment that glucose into ethanol and three significant byproducts, glycerol, acetic acid, and lactic acid.

The invention discloses a microbial composite photosynthetic bacterium agent. The microbial composite photosynthetic bacterium agent consists of the following components in parts by weight: 1 to 3 parts of rhodopseudomonas spheroids, 2 to 5 parts of rhodopseudomonas palustris, 4 to 6 parts of rhodop seudanonas palustris, 2 to 6 parts of anaerobic bacteria, 1 to 3 parts of nitrobacteria, 1 to 6 parts of lactobacillus acidophilus, 5 to 7 parts of nitrosomas, 3 to 5 parts of cellulomonas, 1 to 3 parts of phanerochaete chrysosporium, 2 to 5 parts of white-rot fungi and 3 to 6 parts of bacillus subtilis. The microbial composite photosynthetic bacterium agent is favorable for popularization and application.

The invention discloses a novel composite photosynthetic bacteria agent, which is composed of the following ingredients in parts by weight: 1-3 parts of Rhodospirillum rubrum, 2-5 parts of Streptococcus faecalis, 4-6 parts of Bacillus licheniformis, and aerobic spores 2-6 parts of Bacillus, 1-6 parts of Lactobacillus acidophilus, 5-7 parts of Nitrosobacillus, 3-5 parts of Cellulomonas, 4-8 parts of Bacillus natto, 1-9 parts of Kluyveromyces lactis and 3‑6 parts of Bacillus subtilis. The invention is favorable for popularization and application.