43 results about "Disease mechanisms" patented technology

An analytic apparatus and method is provided for diagnosis, prognosis and biomarker discovery using transcriptome data such as mRNA expression levels from microarrays, proteomic data, and metabolomic data. The invention provides for model-based analysis, especially using kernel-based models, and more particularly similarity-based models. Model-derived residuals advantageously provide a unique new tool for insights into disease mechanisms. Localization of models provides for improved model efficacy. The invention is capable of extracting useful information heretofore unavailable by other methods, relating to dynamics in cellular gene regulation, regulatory networks, biological pathways and metabolism.

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

The present invention relates to identification and uses of biomarkers for neurodegenerative disease, including Alzheimer's disease, and the related diseases. More specifically, the present invention relates to the identification of protein biomarkers useful for the screening, diagnosis, and differentiation of Alzheimer's disease from Parkinson's disease, other neurodegenerative diseases, and normal controls, and in the monitoring of Alzheimer's disease severity and disease mechanisms in patients.

An analytic apparatus and method is provided for diagnosis, prognosis and biomarker discovery using transcriptome data such as mRNA expression levels from microarrays, proteomic data, and metabolomic data. The invention provides for model-based analysis, especially using kernel-based models, and more particularly similarity-based models. Model-derived residuals advantageously provide a unique new tool for insights into disease mechanisms. Localization of models provides for improved model efficacy. The invention is capable of extracting useful information heretofore unavailable by other methods, relating to dynamics in cellular gene regulation, regulatory networks, biological pathways and metabolism.

The present invention is a synthetic microfluidic microvasculature network and associated methods. The synthetic microfluidic microvasculature network mimics the structure, fluid flow characteristics, and physiological behavior of physiological microvasculature networks. Computational methods for simulating flow and particle adherence in synthetic and physiological microvascular systems and methods for determining parameters influencing particle adhesion and drug delivery are also described. The invention has many uses including the optimization of drug delivery and microvascular treatments and in describing disease mechanisms that affect the microvasculature such as inflammation, diabetes and hypertension.

An analytic apparatus and method is provided for diagnosis, prognosis and biomarker discovery using transcriptome data such as mRNA expression levels from microarrays, proteomic data, and metabolomic data. The invention provides for model-based analysis, especially using kernel-based models, and more particularly similarity-based models. Model-derived residuals advantageously provide a unique new tool for insights into disease mechanisms. Localization of models provides for improved model efficacy. The invention is capable of extracting useful information heretofore unavailable by other methods, relating to dynamics in cellular gene regulation, regulatory networks, biological pathways and metabolism.

A synthetic microfluidic microvasculature network and associated methods mimic the structure, fluid flow characteristics, and physiological behavior of physiological microvasculature networks. Computational methods for simulating flow and particle adherence in synthetic and physiological microvascular systems and methods for determining parameters influencing particle adhesion and drug delivery are described with applications in the optimization of drug delivery and microvascular treatments and in describing disease mechanisms that affect the microvasculature.

The invention relates to a triple-amplification-technology-based homogeneous-phase label-free method for detecting the activity of telomerase. The method is characterized in that a telomerase extension product is combined with accessory DNA to release triggering DNA, and the triggering DNA can trigger a strand displacement reaction to form a hairpin H1:H2 compound; G-quadruplexes can be formed at the two tail ends of the compound respectively, fluorescence signals of NMM are weak and are remarkably enhanced after the G-quadruplexes are embedded and inserted, and the activity of the telomerase can be sensitively detected by detecting changes of the fluorescence signals of the NMM. According to the method, the strand displacement reaction is sufficiently adopted, and signal amplification can be achieved without assisting of enzymes; meanwhile, formation of the G-quadruplexes is ingeniously adopted, the method in which the activity of the telomerase can be sensitively detected without labeling is constructed, a new concept is provided for diagnosis and treatment of tumor diseases and researching of disease mechanisms, and the method is of great significance in the telomerase-based cancer treatment and diagnosis aspect.

The invention relates to a combined culture cell micro-fluidic chip which comprises a chip layer and a covering layer, wherein the chip layer and the covering layer are combined and comprise a plurality of channels, the middles of the channels comprise communication portions, the depth of the middle channel is shallower than those of other channels, or the opening diameter of the middle channel is smaller than those of other channels, and an inlet / outlet is formed in each channel. The optimal number of the channels is three, the channels on the two sides are symmetrically scattered with the middle channel as the axis, the bottoms of all the channels are horizontal, and the inlet / outlets are formed in the two ends of each channel. The micro-fluidic chip can achieve three-dimensional growth of cells, can construct a tissue critical plane model in the middle, achieves an effect of observing disease mechanisms by injecting medicine, is reasonable in structure and convenient to use, can effectively establish a biological model and can observe the model situation in real time.

The invention discloses an efficient amplifying and culturing method for biliary epithelial cells in rabbit livers and belongs to the technical field of biological medicines. The efficient amplifying and culturing method comprises the following steps of: (1) separating, purifying and culturing the epithelial cells; (2) detecting cell growth lines; and (3) identifying the epithelial cells. The efficient amplifying and culturing method for the biliary epithelial cells, disclosed by the invention, has the advantages as follows: through a multi-step separating method, biliary epithelial cells with higher purities are obtained and a long-time multiplication culturing system for the biliary epithelial cells is established; a fact that the multiplication of the biliary epithelial cells in the livers can be obviously promoted through an Rho kinase inhibitor Y-27632 is first found; and the efficient amplifying and culturing method for the biliary epithelial cells, disclosed by the invention, has the advantages of simpler operation and the like, is suitable for promotion and application and can provide a platform for researches on a plurality of disease mechanisms including biliary atresia, primary biliary cirrhosis and the like.

The invention discloses a kit for detecting child active tuberculosis and an application of the kit. The kit is combined with a bioinformatics method to screen characteristic metabolic products of child active tuberculosis, and a detecting method for the child active tuberculosis is established based on chemical shift of the characteristic metabolic products of child active tuberculosis; experiments show that in verification of 24 samples by the detecting method provided by the invention, the accuracy is 100%, the specificity is 100% and the sensitivity is 100%; in addition, the detecting method is simple and easy to operate and can be used for early detection of child active tuberculosis; the detecting method provides a new approach for clinical diagnosis of child active tuberculosis as well as provides new thoughts for exploring disease mechanisms.

The invention discloses a method for researching differentially expressed proteins of triple negative breast cancer exosomes by using an iTRAQ technology. The method comprises the following steps of (1) extracting and treating exosomes and exosome proteins; (2) carrying out enzymatic hydrolysis and desalination on exosome protein samples obtained in the step (1) to obtain polypeptides; (3) carrying out iTRAQ labelling and mixing on the polypeptides obtained in the step (2); (4) carrying out HPLC separation and LC-MS analysis on a polypeptide-peptide mixture labelled in the step (3); and (5) carrying out bioinformatics analysis and quantitative analysis on the obtained mass spectrometry identification result to obtain the differentially expressed proteins of the triple negative breast cancer exosomes. Research on triple negative breast cancer exosome proteome can be quickly and effectively carried out by combining the iTRAQ technology disclosed by the invention with an MS / MS technology,and a reference is provided for researching a disease mechanism of a triple negative breast cancer and discovering triple negative breast cancer-related biomarkers.

The invention discloses an enzymatic hydrolysate suitable for mouse brain tissue cell separation in any period and an optimization method for realizing low loss of a single-cell suspension. The proportion of living cells in the single-cell suspension obtained by the method is superior to that of the single-cell suspension obtained by the method in the prior art. Due to the advantages, technicians in the field can complete separation of brain tissue cells of mice in all stages and obtain cell suspension with high motility rate, high yield and low impurity ratio, or the separated cells can be continuously applied to subsequent single cell sequencing experiments, and a more real and meaningful result is obtained. And a more complete guarantee is provided for further clarification of disease mechanisms and treatment of diseases.

This invention relates to the advanced material field of the quantum information, it relates to the self assembling bi-stable quantum line array and its production method of nanometer medicine concretely. The invention self assembles antioxidase oxygen free-radical antagonist by the interaction of the inelasticity electron tunnel penetration, the beta-acceptor excitant, the P2 acceptor excitant, the benzene alkane amine calcium antagonist / or purine nucleic acid monomer and their binary , three-element, four -element and five -element compound. The invention quantum line array have regular geometry configuration, the dimension is controllable and adjustable, the inelasticity electron tunnel penetration, the quantum bit electricity characteristic and the Kondo effect. The invention not only benefits the study of the innovation medicine of target disease form, but also benefits the study of quantum bio-information sensor new material and nanometer structure magnetism memory (MRAM).

Olmsted syndrome (OS) is a rare genodermatosis. The disease is debilitating and progressive keratoderma and auto-amputation of digits can prevent patients from grasping and walking, and confine them to a wheelchair. New therapeutic options are therefore crucial and are expected from a better understanding of the disease mechanisms. The inventors show an abnormal mTOR pathway activation in OS lesional skin. Topical treatment with 1% Sirolimus shows good tolerance and partial but real efficacy on budding, inflammatory and hyperkeratotic lesions of the sole was observed in the treated patient. Accordingly, the present invention relates to a method of treating Olmsted syndrome in a patient in need thereof comprising administering to the patient a therapeutically effective amount of an mTOR inhibitor.

A new type of kidney miniature organoids based on human embryonic stem cells are prepared and tested as forming cysts in vitro or ex vivo. Assays are developed for screening useful candidate molecules towards inhibiting or treating polycystic kidney disease. This provides a new system for modeling polycystic kidney disease.

The invention provides a micro-fluidic chip which comprises a channel layer and a substrate layer; the channel layer comprises a sample introduction area, a mixing area and a detection area which are sequentially communicated; the mixing area comprises a plurality of mixing units; each mixing unit comprises at least one wide channel and at least one narrow channel, wherein the width of the wide channel is 100-300 microns, and the width of the narrow channel is 1 / 5-1 / 2 of the width of the wide channel; and target samples can be efficiently mixed, and functional areas including mixing, separation and detection are integrated on the chip at the same time, so that the requirements of reaction, separation, detection and the like can be met, and the production or research efficiency can be effectively improved. The obtained micro-fluidic chip can also be applied to the field of plasma lipoprotein and exosome separation, and is beneficial to promoting the application of lipoprotein and exosome in disease mechanism research and diagnosis.

Enteroviruses, such as EV-A71, have been found to utilize hWARS as a cell surface receptor, with expression of hWARS rendering cells susceptible to enteroviral infection. Reduction in hWARS cell surface activity reduces susceptibility to enteroviral infection and provides a mode for treatment or prevention of enteroviral infection and its sequelae. Similarly, expression of hWARS in cultured cellsand animal models provides models for understanding enteroviral disease mechanisms and the development of vaccines and / or pharmaceuticals for preventing or treating enteroviral disease.

The invention relates to a carrier for in-situ analysis of intracellular protein complexes, a preparation method and application thereof. The carrier for the in situ analysis of intracellular protein complexes is used to transport the chemical cross-linking agent used for protein complex analysis to a specific location in the cell through transmembrane delivery, and release the chemical cross-linking agent , allowing in situ cross-linking of protein complexes. Combined with mass spectrometry technology, analyze and analyze the spatiotemporal dynamic changes of intracellular protein complexes. This is of great significance for understanding life processes, revealing disease mechanisms, screening biomarkers, and finding drug targets.

The invention relates to a triple-amplification-technology-based homogeneous-phase label-free method for detecting the activity of telomerase. The method is characterized in that a telomerase extension product is combined with accessory DNA to release triggering DNA, and the triggering DNA can trigger a strand displacement reaction to form a hairpin H1:H2 compound; G-quadruplexes can be formed at the two tail ends of the compound respectively, fluorescence signals of NMM are weak and are remarkably enhanced after the G-quadruplexes are embedded and inserted, and the activity of the telomerase can be sensitively detected by detecting changes of the fluorescence signals of the NMM. According to the method, the strand displacement reaction is sufficiently adopted, and signal amplification can be achieved without assisting of enzymes; meanwhile, formation of the G-quadruplexes is ingeniously adopted, the method in which the activity of the telomerase can be sensitively detected without labeling is constructed, a new concept is provided for diagnosis and treatment of tumor diseases and researching of disease mechanisms, and the method is of great significance in the telomerase-based cancer treatment and diagnosis aspect.

Provided herein are methods for assessing a disease score of a subject suffering from or suspected to be suffering from chronic obstructive pulmonary disease (COPD) or associated disease mechanisms, wherein the disease score represents COPD activity. The disease score can be used to stratify the subject into a specific risk category and can further inform patient management decisions. The methods can involve determining a biomarker signature including four or more biomarkers associated with COPD or COPD mechanisms. The methods can further include supplementing the biomarker combinations with calculation or classification trees based on one or more additional clinical parameters or biomarkers. In some cases, the methods include timing of collection of patient samples with respect to acute event or treatment course.

The invention discloses a method for applying iTRAQ technology to study differentially expressed proteins of triple-negative breast cancer exosomes, comprising the following steps: (1) extracting and processing exosomes and exosome proteins; (2) performing step (1) The obtained exosomal protein sample is subjected to enzymatic hydrolysis and desalting to obtain a polypeptide; (3) iTRAQ labeling and mixing are performed on the polypeptide obtained in step (2); (4) HPLC separation and LC are performed on the mixture of polypeptide peptides labeled in step (3) ‑MS analysis; (5) Bioinformatics analysis and quantitative analysis were performed on the above-obtained mass spectrometry identification results to obtain differentially expressed proteins of triple-negative breast cancer exosomes. The iTRAQ technology provided by the invention combined with the MS / MS technology can quickly and effectively conduct the research on the exosome proteome of triple-negative breast cancer, and provide a reference for studying the disease mechanism of triple-negative breast cancer and discovering biomarkers related to triple-negative breast cancer.

The invention discloses a metabonomics pathway-based myocardial infarction early warning method. The method specifically comprises the following steps of S1, taking in 10 patients with qi deficiency and easy chest distress and urgent breath and 10 healthy and symptomatic healthy people, not intervening in the normal life of the healthy people, and taking salvia miltiorrhiza traditional Chinese medicine formula granules for the patients with qi deficiency; and S2, respectively carrying out morning fasting blood sample collection on the two groups of subjects before and after intervening, settling the collected blood for 1.5 hours, centrifuging for 10 minutes at the temperature of 5 DEG C, and sealing after centrifuging, so that the invention relates to the technical field of medicines. According to the metabonomics pathway-based myocardial infarction early warning method, the nuclear magnetic resonance technology is combined with multivariate statistics to analyze the content change ofserum potential markers related to myocardial infarction resistance of salvia miltiorrhiza, and then metabolic pathway analysis is performed on the potential markers, so early warning of myocardial infarction of a patient is facilitated; and research of medical researchers on disease mechanisms is facilitated.

The present invention provides methods for studying pathogenesis of mammalian viruses. In particular, the present invention provides a nonhuman animal model system for studying disease mechanisms wherein the nonhuman animal model is infected with an animal virus. In a preferred embodiment the animal model is C. elegans and the animal virus is vesicular stomatitis virus (VSV).

The invention provides a manufacturing method of vascularized hepatic lobule for tissue engineering. The manufacturing method comprises the following steps: printing a compatible supportive frame of the hepatic lobule by adopting a 3D printer; inserting seven capillary glass tubes into the frame to form a forming mould of the hepatic lobule; uniformly mixing HepG2 cells and a thermo-sensitive hydrogel solution according to the cell concentration being 10<6> cells / mL, adding the obtained solution into the mould, carrying out irradiation for 30-200 s by adopting ultraviolet light, carrying out collagen cure-crosslinking, and drawing out the capillary glass tubes, thus forming a capillary channel; preparing human umbilical vein endothelial cells with the concentration being 1x10<7> cells / mL, injecting the human umbilical vein endothelial cells into the capillary channel, and carrying out culture, so that the human umbilical vein endothelial cells grow all over the inner wall of the capillary channel, and thus the vascularized hepatic lobule is obtained. The hepatic lobule prepared by adopting the method is good in biocompatibility, and can be used for the screening of liver drugs, the study on disease mechanisms, and the study on the metabolic process of the drugs in the liver.

The invention involves an in -site analysis method and application of an intracellular protein complex.In this method, use the parcel crosslinker carrier to incubate with the cells, use intracellular swallowing, and transport the linked agent to a specific part of the cell, and release the chemical cross -linked agentEssenceSubsequently, the protein extraction method based on ionic liquid was used to extract protein and its complexes, and further combined with mass spectrometry technology to analyze the in situ analysis of intracellular protein complexes.This method is of great significance for resolving the dynamic changes in the time and space of the cells in the cells, understanding the process of life, understanding the process of life, revealing the disease mechanism, screening biomarkers, and finding drug targets.

The invention provides a novel efficient mycoplasma columbinum culture medium FG1. The culture medium is obtained through adding dove serum, and polypeptide T1 as shown in a SEQ ID: 1 sequence based on a conventional culture medium FM-4 of mycoplasma. The mycoplasma columbinum can realize rapid growth and proliferation in FG1 culture mediums, and can achieve very high viable bacteria titer, so that rapid detection and diagnosis of the mycoplasma columbinum are facilitated, rapid separation and resolution of the mycoplasma columbinum are facilitated, the development of mycoplasma columbinum vaccines and treatment medicines is facilitated, and research of disease mechanisms of the mycoplasma columbinum is facilitated.

The microfluidic platform device of the claimed invention makes use of a high throughput lab-on-a-chip format to determine the functional profile of antibodies elicited by infection or vaccination against infection for any pathogen. It can be used to evaluate vaccines, evaluate whether vaccinated individuals show indices of protection, determine whether individuals display resistance or susceptibility to infection, discover new vaccine antigens, discover and test therapeutic interventions, or evaluate mechanisms of disease.

The invention discloses a method for key RNA function mining based on high-throughput experimental data mining. By integrating multiple data to obtain concomitant data sets and combining the concomitant data sets with clinical data sets, the most relevant genes for project research can be identified from a large set of known RNAs, while the function of unknown RNAs can be predicted to better determine the roles the unknown RNAs exercise in life activities, so that an important basis is provided for subsequent aspects of disease mechanisms, drug targets, disease diagnosis and the like.