128 results about "Glume" patented technology

The invention provides a breeding method capable of changing the glume color of rice varieties with yellow glume to brownness. The breeding method comprises the following steps: selecting a target fragment in an extron region of a glume color determining gene OsCAD2, constructing a plant CRISPR / Cas9 targeting recombinant vector, guiding the recombination vector into a rice cell, regenerating the rice cell into seedlings, and sequencing genome target fragments of regenerated strains so as to obtain the strains, two allelic genes OsCAD2 of which simultaneously suffer function deletion mutation. Through phenotype identification, the change of the glume color is realized. Experiments prove that the breeding method can quickly change the glume color of the rice varieties.

The invention belongs to the planting technology of crops and relates to a method for treating the soil of a continuous cropping field of greenhouse strawberry. The method includes the following steps: a. plants are pulled out as soon as the harvest of the greenhouse strawberry is finished during May to July every year and ground surface covering materials like black plastic film, etc. are removed; b. green bran, the glume of which is removed, is scattered, the amount is 500-750 kg per mu, and the rice bran is evenly mixed into the soil by adopting mechanical and manual method for intertillage weeding; c. surrounding ditches around the greenhouse and the interior of the greenhouse are fully irrigated with water, and the soil in the greenhouse becomes saturated by absorbing water; d. the field surface in the greenhouse is covered with undamaged plastic film, the soil around the plastic film is firmly pressed to prevent air from entering until August to September when strawberry is to be planted. The method can eliminate the obstacle of continuous cropping of strawberry, causes no pollution to the environment, and provides better organic fertilizer for the growth of strawberry, thus having good comprehensive economic benefit.

The invention belongs to the technical field of plant gene engineering, and more particularly relates to a paddy rice histone demethyl methylase gene, coded protein and the application thereof. The invention provides a new paddy rice histone demethyl methylase gene OsJMJ706, due to inactivation, heteroplasia of rice flowerthe, heteroplasia or deficit of internal and external glumes, as well as lessening of seeds can be caused on the gene, and by being detected by the method of western blot, the gene is proved to have enzyme activity for removing histone H3K9me3 and H3K9me2 locus methylate. The invention provides nucleic acid sequence and protein sequence of the gene as well as the application thereof.

The invention discloses a breeding method of a rice photo-thermo-sensitive genic male sterile line, belonging to a breeding method of plant seeds. The breeding method comprises the following steps of: (1) selecting an early-ripe indica rice photo-thermo-sensitive genic male sterile line Peiai 64S as a female parent and selecting an early-ripe indica rice photo-thermo-sensitive genic male sterile line Guangzhan 63S as a male parent; hybridizing to obtain F1 seeds; and (2) soaking the F1 seeds, accelerating germination and sowing; carrying out selfing for six generations and selecting a photo-thermo-sensitive genic male sterile line target variety with dense panicle, colorless glume end, short straw and strong lodging resistance. The breeding method provided by the invention has the advantages of moderate plant distribution, short and healthy plants and strong lodging resistance, good out-crossing characteristic, and colorless leaf sheath, glume end and stigma; and according to the breeding method, the purity identification can be easily finished.

The invention discloses a sterilization composition containing prothioconazole and azoxystrobin and an application thereof. The sterilization composition takes prothioconazole and azoxystrobin as main effective ingredients. The sterilization composition can be applied to prevention and treatment of diseases of grains, fruit trees and vegetables, has a relatively high synergetic effect, can be used for overcoming and retarding drug resistance of bacteria with high sterilization speed and long duration, and reducing application cost, and has a prevention and treatment effect remarkably superior to that of a single preparation. The sterilization composition can be used for preventing and treating fungous diseases of crops, especially powdery mildew, banded sclerotial blight, fusarium wilt, leaf spot, rust disease, sclerotinia sclerotiorum, net blotch, scald, botrytis, black spot, brown blotch, black shank, glume blight, net blotch, downy mildew and rice blast, and has an effect better than that of a single preparation.

The invention belongs to a special fertilizer for paddy, in particular to a special fertilizer for paddy with unclosed glumes and a production method thereof. Unclosed glumes cause the reduced yield of the paddy and enormous economic loss. The special fertilizer for the paddy with unclosed glumes comprises the following components in parts by weight: 350-450 parts of organic fertilizer, 140-200 parts of potash magnesium sulphate fertilizer, 80-120 parts of monoammonium phosphate, 160-200 parts of urea and 10-30 parts of heptahydrate zinc sulphate. The production method comprises the following steps of: drying and crushing raw materials of the organic fertilizer; composting and fermenting the organic fertilizer; mixing an inorganic fertilizer; and pelleting, drying, cooling, screening and packing. The invention has the advantages of promoting the glumes of the paddy to close, improving the ripening rate and the yield of the paddy, having wide application range, easily obtained raw materials and easy production, being suitable for fields of paddy with the unclosed glumes all around the world, and improving the content of organic matters and the fertilizer retaining capability of the soil.

The invention discloses a method of shearing glume and flower pesticide to kill masculinity for rice hybrid, with detail steps below: carrying out sexual hybridization with rice of same species, shearing 1 / 4-2 / 5 of the glume of not blooming in the hybrid female parent from the afternoon of last day to today, which shears off the flower pesticide but does not damage the chapiter, spraying clean water immediately to reach effect of killing masculinity; vibrating the rice treated with above steps before pollination to remove the flower pesticide from the chaff, then covering bags, pollinating the pollen of male parent into the glume, and getting the sexual hybridized seed of rice. The invention employs method of shearing plume and flower pesticide to kill masculinity for mass sexual hybridization of rice, which increases the hybridization efficiency, the plume and flower pesticide shearing rate and masculinity killing rate is greater than 98%, and is also characterized by the convenient, fast, and good effect, when comparing with normal sexual hybridization it can get more hybrid seeds and meet the demand of mass hybridization combination, which increases the hybridization efficiency greatly, homologous to the efficiency and effect of employing rice of male sterility line for hybridization.

The invention discloses a method of accessorial appraise for the sample of anti stachys white grain wheat. The method is that select white chondrio wheat as alternate samples. The characteristic is that there are wax powders on the glume, during the wax ripe, naked eye cannot see gap at the link of inside and outside of glume, and there is not synechia between seeds and the inner wall of inside and outside of glume. The method of accessorial appraise for the sample of anti stachys white chondrio wheat bonds the method of normal breed, can quicken the procedure of breed wheat; can select quickly the sample of anti stachys white grain wheat from the heavy product, superior quality and against disease of white grain wheat with other normal measuring method, and plunge it into production quickly.

The invention discloses a method for breeding multiple generations of paddy rice in one year. The method comprises the following steps: taking paddy rice seeds, breaking dormancy, accelerating germination and then carrying out direct seeding; after paddy rice enters a flowering stage, carrying out glume cutting, emasculation and pollination on a female parent; selecting seed-grain or brown rice matured at earliest time as a next-generation parent in a filling and ripening stage; and repeating the previous three steps until multiple generations of the paddy rice are obtained. According to the invention, the method employs breeding and cultivation manners like direct seeding culture, glume cutting and emasculation, germination acceleration and seeding of brown rice, and rapid dormancy breaking and germination acceleration via seed soaking with a nitric acid solution, so the whole growth period of paddy rice is shortened; and thus, breeding process is shortened, breeding of multiple generations of paddy rice in one year is realized, and the problem of a long breeding period in the prior art is overcome.

The invention belongs to the field of biotechnologies, in particular to an axenic germination and culture method of roegneria kamoji plant seeds. The method comprises the following steps: seed preprocessing: selecting plump seeds which are not damaged by diseases and insects, washing the seeds by tap water for 15-20min, and steeping the seeds in sterile water at 2-4 DEG C for 12-30h; then explant sterilizing: peeling off the flower glume of the preprocessed seeds, sterilizing by alcohol with the alcoholic strength of 75 percent for 25-40s, washing by sterile water for 3 times, sterilizing by 0.1 percent of mercuric chloride solution for 1-4min, washing by sterile water for 5 times again, then sucking the moisture on the surfaces of the seeds by sterile filter paper, and then transferring the seeds to a solid culture medium which is an MS;1 / 2MS culture medium; firstly, culturing in a light-proof enclosure, then adopting lighting culture when buds grow to be 1-2 cm in height, and then culturing sound seedlings, namely, the seedlings are transferred to a 1 / 2MS culture medium when the seedlings grows to be 8-10 cm in height so that the height of the seedlings is kept to be 10+ / -2 cm. The invention improves the germination ability of roegneria kamoji plants, increases the germination index from 14.48 to 42.06 and obtains uniform sterile seedlings, thereby satisfying the requirements of seed planting and genetic breeding experiments of roegneria kamoji plants.

The invention provides a method for breeding a male sterile line of a ternary hybrid rice of which sword-like leave vines, stem joints, seed glumes and branches all present an orange red mark property. The process comprises the following step: crossing maintainer lines which present the ternary hybrid rice grass color property with the maintainer lines which present the orange red mark property; and crossing F3 individual plants of which the word-like leave vines, the stem joints, the seed glumes and the branches all present the orange red mark property with the ternary male sterile lines, and selecting plants for backcross successively to obtain the male sterile line of the ternary hybrid rice of which the sword-like leave vines, the stem joints, the seed glumes and the branches all present the orange red mark property after circulating to the ninth generation. The seeds have unique leave vain, stem, seed and branch mark properties, have an anti-counterfeit function, and are easy to identify. The hybrid rice male sterile line cultured by the process can greatly reduce the workload of roguing in a seed production process, improve the purities of the parental seeds and the hybrid seeds and improve the use safety of the hybrid rice.

The invention discloses a culture method of rice anther calluses. The culture method comprises a step of disinfecting a rice ear, and removing tops of glumes; a step of suspending the rice ear, with the rice stalk upward and the rice ear downward, in a receiving flask containing nutrient solution to perform oscillation processing; a step of transferring the receiving flask after the oscillation processing is finished and performing induction culture in the absence of light to obtain the rice anther calluses. A formula of the nutrient solution is that an SK3 basic medium contains 2.5-3 g / L of proline, 0.4-0.6 g / L of glutamic acid, 1.8-2.5 mg / L of 2-4D and 1.8-2.5 mg / L of NAA. The culture method provided by the invention has advantages of anther collection convenience, high collection efficiency, and high inductivity and reduced pollution risk of the calluses.

The invention discloses a rice glume development gene promoter p-TRI1 and application thereof. The promoter provided by the invention comes from ordinary cultivated rice O. sativa L. 93-11 which belongs to Oryza species, and the promoter is a DNA molecule of the following 1), 2) or 3): 1) a DNA molecule shown in Sequence 1 in a sequence table; 2) a DNA molecule which crosses with a DNA sequence limited by 1) or 2) under strict condition and has the promoter function; and 3) a DNA molecule which has more than 90 percent of homology with the DNA sequence limited by 1) or 2) and has the promoter function. The discovery of the promoter and the elucidation of the promoter function have important theoretical and practical significance for studying flower organ development mechanism of rice, especially the glume development mechanism and for breeding rice varieties of specific grain types. The promoter has important theoretical and practical significance for studying the molecular mechanism of rice glume development and for rice grain type molecular breeding. The promoter has wide application and market prospect in the field of agricultural.

The invention relates to a pesticide composition containing prothioconazole and triazoles, which comprises active ingredient A and active ingredient B, wherein active ingredient A is selected from prothioconazole, and active ingredient B is selected from cyproconazole , flusilazole, triconazole, wherein the weight ratio of active ingredient A to active ingredient B is 80:1 to 1:80, made into wettable powder, water dispersible granule, suspending agent, suspoemulsion, Water emulsion, microemulsion, used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust, sclerotinia, net spot, sclerotinia, gray mold, black spot, brown spot, rust , scab, white rot, leaf rust, stripe rust, glume blight, leaf mold and other diseases, it has the characteristics of small dosage, resistance to rain erosion, and obvious synergy.

The invention provides an accelerant for glume opening of a photo-thermo-sensitive genic male sterile rice line under high-temperature stress and a preparing method of the accelerant. The accelerant is prepared from citric acid, ethyl alcohol and a plant growth regulating substance, namely, jasmonic acid methyl ester, the three substances have the supplementary effect after being prepared into a solution, the solution is sprayed in the heading stage and can effectively promote glume opening of the photo-thermo-sensitive genic male sterile rice line under high-temperature stress, and the setting percentage and the seed yield after hybridization of the photo-thermo-sensitive genic male sterile rice line and a restoring line are increased.

The invention provides two pairs of primers for detecting germs of paddy bacterial glume blight. The nucleotide sequences of the primers are SEQ ID NO.1: 5'-ACACGGAACACCTGGGTA-3', SEQ ID NO.2: 5'-TCGCTCTCCCGAAGAGAT-3', SEQ ID NO.3: 5'-GCAGCGGCAAGGAAGACG-3', and SEQ ID NO.4: 5'-GTCGTCGCCCGACGTCTC-3'. The invention also provides a method for detecting germs of paddy bacterial glume blight, which comprises the following steps: separating the germs of paddy bacterial glume blight in samples, using the bacterial DNA as a template, carrying out dual PCR amplification by using the primers, carrying out DHPLC analysis on the amplification products, and amplifying the obtained positive results by the primers BGF3 and BGR3 to obtain positive fragments as molecule specimens for carrying out identification. The invention has good primer specificity and high accuracy and high sensitivity of the detection method, thereby ensuring safety in import and export.

The invention relates to a detection method for hybrid rice glume-split seeds based on acoustic characteristics. The detection method is characterized by acquiring a sound signal by controlling the falling height of the hybrid rice seeds and a distance between a sound sensor and an inclined collision glass plate and realizing the recognition and separation for normal seeds and glume-split seeds of hybrid rice by analyzing the time-domain characteristics and frequency domain characteristics of the sound signal. The detection method comprises the following specific steps: shaking a feeding device to make the hybrid rice seeds fall one by one and impact the collision plate; acquiring the sound signal generated by the collision between the seeds and the collision plate by the sound sensor; transmitting the sound signal to a computer by a data line; displaying and storing the sound sensor by the computer; utilizing digital signal processing software to process sound signal data, thereby acquiring the time-domain characteristic curve of the sound signal and extracting the time-domain characteristic values, including voltage amplitude, short-time energy and average amplitude, of the sound signal; performing Fourier transform on the signal, thereby acquiring a frequency-domain characteristic curve, and extracting the frequency-domain characteristic values, including area of power spectral density, maximum value of power spectral density and minimum value of power spectral density, of the sound signal.

It is intended to provide a process for efficiently producing highly purified hop bract polyphenol using hop bract as the starting material, and foods, drinks, cosmetics and drugs with the use of the highly purified hop bract polyphenol. Namely, a process for producing hop polyphenol which comprises extracting hop bract with an aqueous alcohol solution, concentrating the extract to give a residual alcohol concentration of 0.5 to 2% and then centrifuging and / or filtering the concentrate. This polyphenol is usable in foods, drinks, cosmetics, drugs, etc.

The invention relates to nutrition healthcare food and a production method thereof, in particular to whole-grain sweet buckwheat and a processing technology thereof, which is characterized in that the buckwheat is prepared from husked sweet buckwheat kernels including glume, embryo and endosperm of the kernels. The machining process includes: putting un-cooked sweet buckwheat with shells into a closed container, suddenly opening for decompression after heating and compressing, the grains spraying and exposing, the cracked glume after spray and explosion being attached on the surface of rice flowers, simultaneously finishing puffing, curing and shelling processes, then pressing the husked grains into oatmeal, and pressing and attaching glume bran speck patterns on the oatmeal. The invention has the following beneficial effects: the obtained buckwheat reserves all tissues and nutrient components as much as possible except the hulls of buckwheat kernels, reduces processing steps, lowers energy consumption, lowers cost and obtains unexpected effects of thoroughly separating hulls and completely preserving skin; and the buckwheat has pure taste, complete nutrients, less than 8% of processing loss of rutin, remarkably enhanced healthcare effect and considerable market prospect.

The invention relates to a synergistic bactericidal composition and belongs to the technical field of complex pesticides. According to the synergistic bactericidal composition provided by the invention, fluxapyroxad and octyl bacterial amine are taken as main active ingredients to be compounded with known additives and excipients to form any allowable form, such as water dispersible granules, wet seed dressing agents, emulsifiable concentrate, wettable powder, suspension concentrate, oil formulation, suspoemulsion, coating agents of suspension concentrate, emulsion in water or microemulsion. The synergistic bactericidal composition provided by the invention has a significant effect when being applied to the preparation of medicaments for controlling resistant plant fungal diseases of cereals, fruit tress, vegetables and other crops, when being applied to the preparation of bactericidal medicaments for controlling powdery mildew, gray mold, sclerotiose, rot, rust, glume blight, net blotch, downy mildew, rice blast and sheath blight, and particularly when being applied to the preparation of bactericidal medicaments for controlling rice blast and powdery mildew of cucumbers.

The invention belongs to the technical field of rice crossbreeding, and specifically discloses a sexual hybridization method for rice. The method specifically comprises the three following steps: (1)spraying water and trimming a glume: trimming the glume by about 1 / 3-1 / 2 to break a pollen chamber without injuring a stigma, spraying water before and after trimming the glume; (2) setting appropriate pollination time reference: trimming a rice ear in advance when the large-scale hybridization is conducted, soaking in water, spin-drying, processing in a pocket, taking the rice ear as a signal ear, starting to trim the ear and perform pollination when the rice ear blooms; (3) bagging with a kraft bag: pollinating the rice ear subjected to water spraying and glume trimming in the step (1) aftera male parent blooms; and bagging with the kraft bag after pollinating. The methods of spraying water, trimming the glume, setting the pollination time reference, bagging with the kraft bag and the like are organically combined to form a whole set of novel sexual hybridization method, the hybridization efficiency is effectively improved, and the seed quality is guaranteed.

The invention discloses a bactericidal composition containing pyraclostrobin and difenoconazole and an application thereof. The bactericidal composition takes pyraclostrobin and difenoconazole as main active ingredients, wherein the weight ratio of pyraclostrobin to difenoconazole is (1-70):(1-50). The bactericidal composition can be used for preventing the diseases of cereal, melon and fruit or vegetable crops, has relatively high synergistic effect, overcomes and delays the drug resistance of germs, and realizes high bactericidal speed and long duration while the application cost is reduced and the prevention effect is obviously better than that of using a single agent. The bactericidal composition disclosed by the invention can be used for preventing fungal diseases of crops and particularly can be used for preventing the fungal diseases such as downy mildew, sheath blight, false smut, scab, brown spot, glume blight, leaf blight, rust disease, powdery mildew, early blight, leaf spot disease, net blotch and the like of the cereal, melon and fruit or vegetable crops, and the effect is better than that of using a single agent.

The present invention discloses a sterilization composition adopting fluxapyroxad and difenoconazole as main active components, and an application thereof, and belongs to the technical field of compounded pesticides. According to the present invention, the sterilization composition adopts fluxapyroxad and difenoconazole as main active components, wherein a weight ratio of the fluxapyroxad to the difenoconazole is 1-90:1-90, and the total amount of the fluxapyroxad and the difenoconazole is 2-90% of the mass of the sterilization composition; and the sterilization composition provides significant effects for preparation of drugs for prevention and control of crop resistance plant fungous diseases of cereals, fruits and vegetables, and specifically for preparation of sterilization drugs for prevention and control of powdery mildew, botrytis cinerea, sclerotinia rot, rot disease, rust disease, glume spot of rice, web blotch, downy mildew, rice blast, seedling blight and scab of cucurbits, especially sterilization drugs for prevention and control of pear scab of cucurbits and cucumber powdery mildew. In addition, compared with the single-agent substance, the composition has characteristics of prevention and control spectrum expanding, production and use cost reducing, and drug resistance overcome and delaying.

The invention discloses a method for preparing a multi-element seaweed bio-fertilizer for preventing and controlling unclosing of rice glumes, and belongs to the technical field of fertilizers special for rice. The multi-element seaweed bio-fertilizer takes the following components as raw materials: seaweed meal, compound sodium nitrophenolate, urea, potassium sulphate, monoammonium phosphate, a calcium magnesium phosphate fertilizer, calcium silicate, EDTA chelated zinc, EDTA chelated boron, and a microorganism bacterium agent. Compared with the prior art, the product provided by the invention is natural, green and pollution-free, and can effectively prevent and control unclosing of the rice glumes; the trace elements, such as calcium, magnesium, sulphur, silicon, zinc and boron, are added so as to promote the closing of the rice glumes; the stress resistance of the crops and the fertilizer utilization rate are improved; soil is improved; soil fertility is fertilized; the quality of the crops is improved.

The invention belongs to the technical field of pesticide and relates to a broad spectrum fungicide, in particular to a farming fungicide comprising Azoxystrobin. The invention consists of the original pesticide of the Azoxystrobin, an accessory ingredient and an excipient; and microemulsion and missible oil are manufactured by a normal production technique. The Azoxystrobin is a methoxy acrylate fungicide developed by Syngenta Company, is used for controlling powdery mildew, rust disease, glume blight, scab, downy mildew and Magnaporthe grisea on cereal crops, fruit trees and vegetables, and has specific effects on powdery mildew of cucumber, downy mildew of grape and apple scab.

The invention belongs to the field of plant genetic engineering. Six upstream promoters (EnP3, EnP3-859, EnP3-678, EnP3-471, EnP3-292 and EnP3-110) with different lengths of the same endosperm specific expression gene (access number: GI 3141524) are cloned from a rice genome; and the nucleotide sequences of the promoters are nucleotide sequence SEQ ID No.1, nucleotide sequence SEQ ID No.2, nucleotide sequence SEQ ID No.3, nucleotide sequence SEQ ID No.4, nucleotide sequence SEQ ID No.5 and nucleotide sequence SEQ ID No.6. Five promoters (EnP3, EnP3-859, EnP3-678, EnP3-471 and EnP3-292) are expressed only in endosperms of transgenic plants, and the expression of the five promoters reduces as the endosperms grow; and the expression of the five promoters can be detected in none of leaf blades, leaf sheaths, stalks, roots, flowers, glumes and embryos. The expression of the promoter EnP3-110 can detected in green tissues such as the leaf blades, leaf sheaths, stalks, and glumes, but not in the roots, flowers and seeds (including embryos and endosperms), so the promoter EnP3-110 is a short green tissue specific expression promoter. The invention also discloses a method for preparing the six promoters and the corresponding expression vectors thereof and the use of the six promoters through the transfer of the six promoters into rice by using transgenic method of agrobacterium rhizogenes mediation.

The invention discloses a bactericidal composition containing picoxystrobin and procymidone. The bactericidal composition comprises active ingredients, namely picoxystrobin and procymidone which are compounded, and the balance being auxiliary ingredients, wherein a mass ratio of picoxystrobin to procymidone in the pesticide composition is (1-50):(50-1); the mass part ratio of the active ingredients, namely picoxystrobin and procymidone, in the preparation is 1-80 percent, and the auxiliary ingredients are pesticidally permitted and acceptable. The bactericidal composition has the dosage forms such as missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and soluble concentrate, is mainly used for preventing and treating foliar diseases of wheat, such as leaf blight, brown leaf rust, glume blight, brown blotch and powdery mildew and has good effects on gray mold and stalk break of fruit trees, vegetables and crops.

The invention discloses a sterilization composition containing prothioconazole and trifloxystrobin and an application thereof. The sterilization composition takes prothioconazole and trifloxystrobin as main effective ingredients. The sterilization composition can be applied to prevention and treatment of diseases of grains, fruit trees and vegetables, has a relatively high synergetic effect, can be used for overcoming and retarding drug resistance of bacteria with high sterilization speed and long duration, and reducing application cost, and has a prevention and treatment effect remarkably superior to that of a single preparation. The sterilization composition can be used for preventing and treating fungous diseases of crops, especially powdery mildew, banded sclerotial blight, fusarium wilt, leaf spot, rust disease, sclerotinia sclerotiorum, net blotch, scald, botrytis, black spot, brown blotch, black shank, glume blight, net blotch, downy mildew, rice blast and apple scab, and has an effect better than that of a single preparation.