249 results about "Hematology analyzer" patented technology

An acoustic blood analyzer with a transducer section of acoustic biosensors for measurement of blood properties including, but not limited to blood coagulation, platelet function and various blood disorders in a blood sample is provided. Methods for use of the blood analyzer in measurement of blood properties are also provided.

A method and apparatus for use in respect of samples which are assessed for quality prior to testing in a clinical analyzer. The method and apparatus identify parameters such as gel level and height of fluid above the gel in blood samples, where appropriate, for the purposes of positioning the specimen for determination of interferents. Such interferents include hemoglobin (Hb), total bilirubin and lipids. These interferents are determined by measurement of absorption of different wavelengths of light in serum or plasma, or other specimens, which are then compared with values obtained through calibration using reference measurements for the respective interferents in serum or plasma or other type of specimen. Determinations of temperature of the specimen, as well as specimen type, for example whether the specimen is urine or plasma or serum, may also be carried out.

A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d.c. impedance volume, conductivity / opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements. Novel methods for use of the reagents with automated and semi-automated hematology analyzers are also provided.< / PTEXT>

A hematology analyzer, obtains red blood cell scattered light information which is scattered light information related to red blood cells contained in a blood sample and reticulocyte scattered light information which is scattered light information related to reticulocytes contained in the blood sample; obtains a value equivalent to the amount of hemoglobin in the red blood cells from the red blood cell scattered light information; obtains a value equivalent to the amount of hemoglobin in the reticulocytes from the reticulocyte scattered light information; obtains difference between the hemoglobin amounts which is the difference between the value equivalent to the amount of hemoglobin in the red blood cells and the value equivalent to the amount of hemoglobin in the reticulocytes; and obtains information supporting clinical examination based on the value equivalent to the amount of hemoglobin in the reticulocytes and the difference between the hemoglobin amounts, is disclosed. A hematology analyzing method and a computer program product are also disclosed.

A method for enumerating white blood cells and nucleated red blood cells. The method comprises the steps of:(b) providing a lysed sample of whole blood;(b) introducing the lysed sample to a light-scattering multi-angle depolarizing flow cytometer;(c) removing depolarizing interference, e.g., lipid droplets and other measured particles;(d) differentiating nucleated red blood cells and noise from white blood cells in the absence of depolarizing interference;(e) differentiating nucleated red blood cells from noise in the absence of depolarizing interference and white blood cells; and(f) differentiating possible platelet clumps.

A disposable cassette for blood analysis includes a housing having an upper panel and a sampling section having a filling inlet; at least one pair of chambers in a form of depression of the upper panel of the housing and sealed by a diaphragm; portions of the diaphragm over the chambers being flexible; and one or more channels adapted to interconnect the pair of chambers; one of the chambers containing a predetermined amount of a reagent for blood analysis; and a sample outlet disposed next to and connected to the chamber containing the reagent, the sample outlet including an outlet cavity recessed from the upper panel, a divider disposed therein, and a cover covering the outlet cavity; the sample outlet sealing the reagent to the chamber containing the reagent. Further disclosed is the method of using the disposable cassette for measurements of hematology parameters on a blood analyzer.

The invention discloses a full-automatic testing tube liquid sample blending device and a hematology analyzer. The full-automatic testing tube liquid sample blending device mainly comprises an X-directional horizontal feeding mechanism, a Z-directional lifting mechanism and a Y-directional uniform mixing mechanism, wherein the X-directional horizontal feeding mechanism is used for driving a test tube to realize linear horizontal movement in an X direction; the Z-directional lifting mechanism is used for driving the test tube to realize linear lifting movement in a Z direction; the Y-directional uniform mixing mechanism is used for driving the test tube to realize rotary deflection movement in a YZ plane around an X axis; the Z-directional lifting mechanism is used for driving upper and lower dragging brackets by a motor and a synchronous belt to do Z-directional vertical lifting movement along a first linear guide rail; the Y-directional uniform mixing mechanism is driven by supporting rods in the upper and lower dragging brackets to do vertical lifting movement on a third linear guide rail; the X-directional horizontal feeding mechanism is used for driving a horizontal movement plate on a second slide block by the motor and the synchronous belt to do the linear horizontal movement on a second guide rail in the X direction; and the Y-directional uniform mixing mechanism is arranged on the horizontal movement plate. With the adoption of the full-automatic testing tube liquid sample blending device, the manufacturing precision requirements are easily reduced, and the cost is low due to few parts and a designed metal plate.

A device for enabling the user of a clinical analyzer, such as, for example, an automated clinical analyzer, e.g., an automated hematology analyzer, to identify samples that require additional processing subsequent to an initial run through the clinical analyzer. The device can also indicate the location of sample containers to assist the user in finding a sample from a sample retention area. The device comprises a rack comprising a plurality of receptacles, each receptacle having a recessed area for holding a sample container, e.g., a sample tube. Each receptacle is associated with an indicator for signaling when a sample container in a given receptacle area requires additional processing.

A blood analyzer, comprising a sample preparing part; a flow cell; a light source; a scattered light detector for detecting scattered light from a measurement sample irradiated by the light source; a fluorescence light detector comprising an avalanche photo diode for detecting fluorescence light from the measurement sample irradiated by the light source; a signal processing part for processing a first detection signal from the scattered light detector and a second detection signal from the fluorescence light detector, wherein the signal processing part reduces high frequency noise included in an amplified second detection signal; and a analysis part for classifying white blood cells in the blood into groups based on the first and the second detection signals processed by the signal processing part is disclosed. A blood analyzing method is also disclosed.

A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

A blood analyzer comprising: a specimen preparation section for preparing a measurement specimen that is used to measure a white blood cell count among CBC measurement items; a measurement section for obtaining optical information from blood cells contained in the measurement specimen; and a controller carrying out operations comprising: classifying blood cells contained in the measurement specimen into at least white blood cells and blood cells suspected to be abnormal blood cells based on the optical information; obtaining distribution data of the white blood cells and distribution data of the blood cells suspected to be abnormal blood cells; counting the white blood cells based on the distribution data of the white blood cells; and determining presence or absence of the abnormal blood cells in the blood sample, based on the distribution data of the blood cells suspected to be abnormal blood cells. A blood analyzing method and a computer program product are also disclosed.

A method of making a reference control containing a nucleated red blood cell component includes providing a blood cell containing a nucleus; treating the blood cell with a treatment solution to alter a nucleus property from a natural value to a target value suitable for simulating nucleated red blood cells on a blood analyzer; and suspending treated blood cell in a suspension medium to form a reference control. The method also includes integrating the nucleated red blood cell component with white blood cell, red blood cell, platelet and reticulocyte components. Further disclosed is a cell treatment composition for altering a nucleus property, which includes a conditioning component, a lytic component for permeating cell membrane, and a fixing component for preserving the cell nucleus. Also disclosed is a method of using the reference control for measurement of nucleated red blood cells on a blood analyzer.

The invention discloses a blending device for a hematology analyzer. The blending device comprises idler wheels which can rotate for 360 degrees and a roller for bearing a test tube stand, wherein the roller and the idler wheels are fixed together; the inner part of the roller is provided with a space for containing the test tube stand; a plurality of test tubes can be contained on the test tube stand. When the blending device works, a plurality of the test tubes are contained on the test tube stand and the test tube stand is arranged in the roller; the idler wheels rotate and drive the roller to rotate for 360 degrees or 180 degrees, so that blood samples in the test tubes can be uniformly blended. The blending device disclosed by the embodiment of the invention can carry out shaking-up operation on a plurality of the test tubes in the test tube stand for one time so that times for grabbing the test tubes can be reduced by shaking up a plurality of the test tubes for one time when being compared with a shaking-up device in the prior art, and the quantity for shaking up the test tubes for one time is increased; therefore, the blending efficiency of the hematology analyzer is improved.

A hematology reference control containing an immature granulocyte component and the method of making are disclosed. The reference control includes an immature granulocyte component made of processed blood cells for simulating human immature granulocytes, and a suspension medium suitable for delivering the component to a blood analyzer for measurement of immature granulocytes. The immature granulocyte component can be made of processed avian, reptile or fish red blood cells, or human immature granulocytes produced in vitro. The reference control further includes a white blood cell component which includes white blood cell sub-components for simulating white blood subpopulations. Further disclosed is the method of using the reference control for measurement of immature granulocytes on a blood analyzer.

In a blood analyzer for separating plasma in a flow channel by centrifugal operation, it is intended to effectively utilize a whole blood sample supplied into the flow channel, shorten the flow channel and reduce the apparatus size. It is also intended to reduce the amount of the blood to be collected, thereby reducing the burden on a subject. A blood cell reservoir wherein blood cells are precipitated is provided in a flow channel of a blood analyzer along the centrifugal direction upon centrifugation. Then blood cells are cumulated in the reservoir by centrifugation so that the plasma fraction is allowed to continuously exist in both of the upstream and downstream sides of the U-shaped flow channel without being divided by the blood cell fraction. Thus, the plasma in a required amount can be fed into the analysis means using a smaller amount of the whole blood. Therefore, the whole blood can be utilized more efficiently, which is suitable for the shortening of the flow channel and the reduction of the device size. The plasma, which is not divided by the blood cell fraction, can be transferred due to a lower negative suction pressure. Thus, the pump capacity required in drawing the plasma can be reduced, which contributes to the size reduction and cost down of peripheral devices.

A method for identifying a platelet population, preferably a population of immature, reticulated platelets, in a biological sample involves incubating the biological sample for less than minutes with at least one labeled, ligand (e.g., monoclonal antibody) that binds to an epitope or antigen on platelets and with a nucleic acid dye. In one embodiment, the dye is Acridine Orange and the label on the ligand is PE-Cy7. The sample is then analyzed and one or more platelet populations is rapidly identified or quantified by passing the incubated sample through a sensing region of a flow cytometer. In one embodiment, this method occurs without a washing or physical cell separation step. The incubated sample is irradiated with a laser light source, and fluorescence of the labeled ligand and the nucleic acid dye are measured along with at least one additional parameter, e.g., light scatter, direct current, axial light loss, opacity, radio frequency, and fluorescence. These parameters are used to identify qualitatively or quantitatively the platelet populations in the sample. This rapid analytic method is particularly valuable in clinical situations where either low platelet counts or interfering conditions lead to inaccuracies of the platelet measurement. This method is suitable for performance in an automated hematology analyzer.

The invention discloses a blood analysis meter test tube detection device comprising a support and a photoelectric sensor assembly, wherein the photoelectric sensor assembly comprises an optical emitter and an optical receiver; the optical emitter and the optical receiver are connected to the support, and a test tube cap is located on the sensing paths of the optical emitter and the optical receiver. The invention also discloses a test tube detection method which is characterized in that after a test tube reaches a detection site, the test tube cap changes a signal sent out by the emitter, the receiver receives the signal and judges that a test tube exists in the detection site according to the received signal. In the invention, the self lightproof test tube cap of the test tube is utilized to stop or reflect light beams to implement test tube detection and other lightproof elements do not need to be additionally arranged to stop or reflect light, thereby simplifying the structure of the test tube detection device, and the reliability is high; meanwhile, the optical emitter and the optical receiver of the photoelectric sensor assembly are fixed in the direction which forms a specific angle with the test tube through the support, thereby ensuring that more test tubes with different dimensions can be compatible.

The invention provides a blood analyzer comprising a blood sample supply section, a sample preparation section for preparing an assay sample by mixing the blood sample with a nucleic acid-staining fluorescent dye, a light source for irradiating the assay sample, an optical detecting section for receiving fluorescence emitted from the irradiated assay sample and a controller for performing operations comprising detecting a cell group comprising lymphoblasts on the basis of the fluorescence received by the optical detecting section, and outputting an information on an appearance of the lymphoblasts in the blood sample, as well as a method for determining the existence and nonexistence of lymphoblasts in a blood sample.

A method and apparatus for use in respect of samples which are assessed for quality prior to testing in a clinical analyzer. The method and apparatus identify parameters such as gel level and height of fluid above the gel in blood samples, where appropriate, for the purposes of positioning the specimen for determination of interferents. Such interferents include hemoglobin (Hb), total bilirubin and lipids. These interferents are determined by measurement of absorption of different wavelengths of light in serum or plasma, or other speciments, which are then compared with values obtained through calibration using reference measurements for the respective interferents in serum or plasma or other type of specimen. Determination of temperature of the specimen, as well as specimen type, for example whether the specimen is urine or plasma or serum, may also be carried out.

A method for directly illuminating a Coulter aperture so light scattering can be incorporated into the classical Coulter aperture and 3-diff blood analyzer to realize full functional hematology analyzer by providing a flow chamber which is separated into two portions by a plate having a Coulter aperture; and using a light source to directly coaxial illuminate the Coulter aperture wherein the illuminating beam propagates in the same direction as the axis of the Coulter aperture and the cell's moving direction.

A method for distinguishing erythroblasts from bacteria by automated hematology analyzers, such as, for example, the CELL-DYN® 4000 automated hematology analyzer and the CELL-DYN® Sapphire™ automated hematology analyzer. Bacterial cells scatter light and fluoresce differently than do red blood cells, white blood cells, erythroblast nuclei, and platelets. Signals generated by bacteria are distinguishable from those of erythroblasts because the signals generated by erythroblast nuclei are sufficiently unique that erythroblast nuclei can be distinguished from signals generated by bacteria. Signals generated by platelets, lysed red blood cell ghosts, and other cell debris are blocked by the triple-trigger circuitry of the hematology analyzer, because all of the signals generated by noise are below the AND / OR thresholds. Algorithm(s) in the software of the system detect and count signals generated by bacteria by means of the location and the shape of the signals generated by bacteria and calculate the concentration of bacteria per unit of body fluid. In addition, certain body fluids, such as, for example, synovial fluid, can be pretreated with a viscosity reducing agent for a short period of time to reduce the viscosity of the body fluid prior to analyzing a sample of the body fluid by an automated hematology analyzer.

A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.

A corpuscle / plasma separating part is disposed at the lower end of the substrate, and a sensor part connected to the corpuscle / plasma separating part is disposed at the upper end of the substrate, with a calibration solution reservoir being disposed on the lower side of the sensor part, and a calibration solution waste reservoir being disposed on the upper side of the sensor part. A first centrifugal axis is located upper to the corpuscle fraction storing part and lower to the plasma fraction storing part of the corpuscle / plasma separating part, while a second centrifugal axis is located within or close to the sensor part. Conveyance and discharge of the calibration solution can be carried out by performing centrifugation around the first centrifugal axis which is distant from the sensor part at a low speed of rotation, so that the centrifugal force exerted on the sensors would be small. During the centrifuge operation at a high speed of rotation for the separation of blood corpuscles, centrifugation can be performed around the second centrifugal axis so that the centrifugal force exerted on the sensors is small. Centrifuge operation allows separation of the blood corpuscles and blood plasma, and conveyance of the blood plasma and the calibration solution, as Well as certain discharge of the calibration solution from the sensors, thereby allowing precise analysis. Any damage in the sensors due to strong centrifugal force during the separation of blood corpuscles and blood plasma can be prevented.

The embodiment of the invention discloses a self-diagnosis method for the measure result of a blood analyzer, and a device thereof. The method comprises the following steps: generating a sample sucking process light transmittance curve according to the change of the sample light transmittance in the blood sample sucking process; judging whether an abnormity appears in the sample sucking process or not according to the sample sucking process light transmittance curve; determining that the measure result of the blood sample can be trusted if no abnormity appears in the sample sucking process; and determining that the measure result of the blood sample is doubtful if the abnormity appears in the sample sucking process. The self-diagnosis method for the measure result of the blood analyzer, and the device thereof have the advantages of improvement of the measure result self-diagnosis accuracy of the blood analyzer, reduction of the false positive rate, reduction of the work intensity for the measure result user check, and measure cost saving.

A disposable cassette for blood analysis includes a housing having an upper panel and a sampling section having a filling inlet; at least one pair of chambers in a form of depression of the upper panel of the housing and sealed by a diaphragm; portions of the diaphragm over the chambers being flexible; and one or more channels adapted to interconnect the pair of chambers; one of the chambers containing a predetermined amount of a reagent for blood analysis; and a sample outlet disposed next to and connected to the chamber containing the reagent, the sample outlet including an outlet cavity recessed from the upper panel, a divider disposed therein, and a cover covering the outlet cavity; the sample outlet sealing the reagent to the chamber containing the reagent. Further disclosed is the method of using the disposable cassette for measurements of hematology parameters on a blood analyzer.

The invention uses synergetic combination effect of multiple cytoactive protection solution and a stabilizer, and a protective agent provides nutrition components and living environment required by maintaining normal physiological activity for blood cells for anaerobic glycolysis; the stabilizer containing cytolemma and cytoplasm fixed substances with lower concentration can slowly permeate the cytolemma to act on the cytoplasm and firmly combine with biomolecule of cell components such as protein, enzyme, lipid and the like effectively, thus enabling white blood cells, red blood cells and blood platelets to be fixed in different degrees and get effective protection and enabling blood cells to keep apparent morphological characteristics thereof. The blood cells in protection and good stabilized treatment is subjected to assignment according to traceability link delivery procedures and reference methods thereof, and can be used as blood cell analyzer calibrators for clinic usage. The invention uses stem complete blood cell (CBC) from human beings or animals, thus having good stability; the invention can be used in microscope manual counting and cell staining analysis, and has longerexpirydate compared with the current full blood cell calibrators.

The invention discloses a thermal analyzer and flowing carrier gas-single-drop microextraction combined device and a using method thereof. A material is thermally decomposed to release a gas in a thermal analyzer, the component gas passes through a metal adapter, is vertically discharged upwards through an air vent at the upper end of an adapter under the driving of flowing carrier gas, and is extracted by single drop above the air vent, and escaping components are enriched and concentrated during extraction. The invention has the characteristics that: 1, sample pretreatment technology of single-drop microextraction integrating rapidness, efficiency, low cost and low solvent consumption is applied to analysis of the escaping components of the thermal analyzer under the driving of the carrier gas; 2, the sample pretreatment is performed by the combined device, and by combining other analyzers, giving the information of the escaping components of the thermal analyzer and combining thermal analysis data, material thermal degradation can be comprehensively characterized; and 3, the thermal analyzer and flowing carrier gas-single-drop microextraction combined device has good extractioneffect, and is simple, convenient to operate, and conveniently combined with the other analyzers.