93 results about "Immune resistance" patented technology

Embodiments of the invention are directed to methods of treating, inhibiting or attenuating a microbial infection in an individual who has or is at risk for developing such an infection, comprising the step of administering an effective amount of a TLR9 agonist and a TLR 2 / 6 agonist to the individual.

The invention provides Lactobacillus rhamnosus M9 separated from breast milk. The microbial preservation number is CGMCC No.16002, and the Lactobacillus rhamnosus M9 has the excellent tolerance of gastrointestinal fluid and the good cholate tolerance, and has the basic condition as probiotics. The Lactobacillus rhamnosus M9 has the effect of enhancing immunity resistance, improving intestinal flora diversity and enhancing the intestinal flora stability, and the Lactobacillus rhamnosus M9 can be added into various common foods, healthcare products and the like as probiotics.

The invention discloses application of a plant immune resistance inducer in prevention and control of damping-off and blight of cucumbers. The plant immune resistance inducer is prepared from the following active ingredients in percentage by weight: 0.001 to 0.025 percent of amino-oligosaccharin, 0.01 to 0.25 percent of phosphite and 0.01 to 0.25 percent of phosphate. Each component of the immune resistance inducer obtained by combining the amino-oligosaccharin, the phosphite and the phosphate is mixed in a reasonable proportion, so that a good prevention and control effect on damping-off and blight of the cucumbers can be achieved, an emergence rate and a seedling rate of the cucumbers can be increased, and seedling quality of the cucumbers can be improved.

The present invention relates to moist soft granulated bait feed and a preparation method thereof. Specifically, the bait feed is prepared by controlling a pH value and water contents to make the bait feed stable in a water body so that the bait feed is not prone to collapse and scatter; and extruding and granulating materials to make the materials have a certain mass density and granulated dosage regimen characteristics, which makes the bait feed convenient to throw and easy for animals to hold. The bait feed is comprehensive in nutrition and rich in active substances. Compared with the dry granulated feed, the bait feed can avoid problems of loss of nutritional ingredients and inactivation of lactic acid and other active substances in granulating, processing, curing, drying and other process. The bait feed is easy to eat, digest and absorb, and can save resources, reduce water pollution, enhance immune resistance to disease for shrimps

Provided is a preparation formula prepared by combining fatty acid or fatty acid salts or derivatives thereof with chitosan and the like. The composition has significant effects on enhancing animal immunity resistance, inhibiting tumor growth, enhancing an animal anti-stress ability, increasing animal appetite and promoting animal growth, and has positive significance in reduction of livestock dependence on antibiotics, hormones and the like.

The invention relates to a construction method of a muscle cell line of anabarilius grahami and belongs to the technical field of freshwater organism cell culture. The method comprises the steps of firstly preparing cell culture fluid, ensuring that anabarilius grahami muscle tissue serves as a material, inoculating in a culture bottle of 25 square centimeters, and placing a culture box at a temperature of 28 DEG C for culture; replacing the culture liquid in half volume every 3 days, and performing passage by using a trypsin digestion method after a cell grows into a monolayer; and performing passage every 5 days, when the passage of a tenth generation is achieved, the serum concentration is the culture fluid is reduced to 10%, and the cell line is constructed successfully. The method has the advantages that 1, the operation is easy and feasible; 2, in the constructed anabarilius grahami muscle cell line (AGM), the cell growth is good, the cells are in a fibroblast cell pattern, above 50 generations can be passed to continuously, and the method can be applied to studies of anabarilius grahami cell biological characteristics and immune resistance gene functions directly; and 3, the method is also applicable to muscle cell lines of other fishes.

The invention discloses an appetizing toon pig feed, which is prepared by the following active ingredients in parts by weight: 100-110 parts of corn, 40-50 parts of soybean, 15-20 parts of peanuts, 20-30 parts of manioc waste, 20-30 parts of soybean straws, 7-9 parts of bean paste, 1-2 parts of spina date seed, 1-2 parts of clove, 2-3 parts of shrubalthea flower, 10-15 parts of red jujube, 8-10 parts of seaweed meal, 10-12 parts of eggshell meal, 1-2 parts of kaolin, 20-25 parts of white gourd, 8-10 parts of fresh toon, 4-5 parts of auxiliaries, and a proper amount of water. Sugar, protein, mineral substances, amino acid and vitamins and medium trace elements and the like in the pig feed are completely matched, the immunity resistance capability of pigs can be enhanced under the condition of no addition of any antibiotics, the meat with no chemical drug residues can be produced, the appetite of the pigs can be increased, the food intake of pigs can be increased, the disease resistance can be enhanced, and the nutrition is balanced. The added toon contain volatile aromatic organic matters such as toonanin, thus being capable of strengthening spleen and promoting appetite, and also has the effects of clearing heat and promoting diuresis, and being diuretic and detoxifying.

The invention discloses an anguilla anguilla liver cell line and a construction method and application thereof, and belongs to the technical field of sea fish cell culture. The cell line is preserved in the Chinese typical culture preservation center on December 16, 2014, and the serial number of preservation registration is CCTCC NO: C2014239. The anguilla anguilla liver cell line is fibroblast cells, the continuous passage achieves more than 60 generations, the adherence after cryopreservation resuscitation is good, the growth is stable, the liver cell line can be directly used for research on aquatic virology, anguilla anguilla cell biological characteristics and immune resistance gene function, and the lever cell line has been proved to be sensitive to eels herpes virus. According to the anguilla anguilla liver cell line and the construction method and application thereof, the constructing method is simple and convenient to implement, the repeatability is high, and the cost is moderate. The anguilla anguilla liver cell line and the construction method and application thereof can also be applied to the preparation of other anguilla anguilla cell lines.

The invention provides a plant immune resistance inducer containing amino-oligosaccharin, which is prepared from the following raw materials in parts by weight: 1-3 parts of amino-oligosaccharin, 0.8-3.5 parts of brassinolide, 0.01-0.1 part of sodium monofluorophosphate, 0.9-2 part of 3-(difluoromethyl)-1-methyl-N-(3, 4, 5-trifluorobiphenyl-2-yl) pyrazole-4-formamide, linear polysaccharide beta-1,0.1-0.8 part of 4-xylan, 0.5-1.5 parts of a trace metal ion compound, 10-20 parts of a nutrient activator and 0.8-3.0 parts of a synergist. The composition is reasonable in formula, easy to prepare,high in storage stability and low in effective component content loss rate; the plant immune resistance inducer containing amino-oligosaccharin is applied to tobacco damping-off, can promote crop health or growth and development, has a relatively strong prevention and treatment effect on tobacco damping-off, and has an inhibition rate of 97.99.6%.

The invention discloses a nicotinamide mononucleotide and herbal extract microcapsule with limited component proportion. The capsule can maximize the immune resistance function. The preparation devicecomprises a container for accommodating nicotinamide mononucleotide, herbal active extract and an extraction solvent, and a return pipe, wherein the return pipe is provided with a feeding pipe, a delivery pipe and a connecting pipe; and a necking assembly is arranged in the connecting pipe. The device also comprises an ultrasonic assembly arranged in the connecting pipe, which can generate ultrasonic waves to quickly and uniformly mix the nicotinamide mononucleotide and herbal active extract in the mixed solution. The novel preparation process is utilized to uniformly fuse the herbal plant concentrated solution and excipient / emulsifier by a microencapsulation technique, thereby being different from the traditional excipient or emulsifier which is only coated on the outer layer, enhancingthe functional component proportion, lowering the excipient content and increasing the bioavailability requirements of the nicotinamide mononucleotide and herbal active extract.

The invention relates to a pharmacological substance based on biologically active substances obtained from the plant Tribulus terrestris L. to be used as an agent reducing blood sugar, improving blood circulation and especially blood circulation in veins and capillaries of the limbs of diabetic patients, reducing bad cholesterol level, increasing good cholesterol concentration, maintaining cardiovascular and liver functioning with an additional prophylactic or healing effect on the immune system and the immune resistance. The pharmacological substance represents a combination of bio-active trivalent chromium and a complex of steroid saponins obtained from the plant Tribulus Terrestris l. and consisting of furostanols, spirostanols, sapogenins, sterols, flavonoids and other biologically active substances typical of this plant.

Embodiments of the invention are directed to methods of treating, inhibiting or attenuating a microbial infection in an individual who has or is at risk for developing such an infection, comprising the step of administering an effective amount of a TLR9 agonist and a TLR 2 / 6 agonist to the individual.

The invention provides stevia rebaudiana-containing feed and application thereof, and belongs to the technical field of feed. The stevia rebaudiana-containing feed contains original plants of stevia rebaudiana and / or stevia rebaudiana extract, and the stevia rebaudiana original plants are stalks and / or leaves. The stevia rebaudiana extract is prepared by fermentation and cooking of stevia rebaudiana extraction liquid. The stevia rebaudiana extract is rich in antioxidant components, effectively strengthens the immune system functions of a body, effectively reduces the degree of oxidation of fatand cholesterol in muscle, improves the intestinal flora of livestock, increases appetite, and is used for promoting the growth and development of the livestock and / or improving the meat quality of the livestock and / or enhancing the autoimmunity of the livestock and improving the delivery performance of female livestock or improving the growth / productivity of young livestock after birth. The stevia rebaudiana extract can further improve the effects of histamine allergy on the growth of aquatic products, improves the hypoxia tolerance of the aquatic products, and is used for repairing histamine damage to aquatic organisms or improving the antiviral ability of the aquatic organisms or improving the hypoxia tolerance of the aquatic organisms or promoting the aquaculture growth of the aquaticorganisms.

The invention provides a novel gene, which is separated from cynoglossus semilaevis and related to immune. The gene is subjected to in-vitro recombinant expression to obtain a recombinant expression product, and the antipathogen activities and functions of the recombinant expression product are identified to provide a gene resource and technical method for cynoglossus semilaevis disease control, green feed additives and antibacterial agents. The gene codes the following proteins: (a) protein with an amino acid sequence of SEQ ID NO:2; (b) protein including amino acids from the 117th-255th bits in (a) and has functions of (a); and (c) protein which is substituted, deleted or added by one or more amino acids in the amino acid sequence of (a) or (b) and has the protein activity in (a). The gene is closely related to immune resistance, the gene expression quantity of the gene is related to pathogenic stimulation, and the in-vitro recombinant protein of the gene can remarkably inhibit the proliferation of gram-negative bacteria, positive bacteria and viruses, so that the gene has application potentials in cynoglossus semilaevis disease control, green feed additive and antibacterial agents.

The invention discloses an immune inducer for improving plant antiviral ability, wherein the immune inducer comprises auxiliary materials and also comprises the following components in parts by mass:10-25 parts of lentinan, 20-40 parts of a synergist, 5-6 parts of a wetting agent, 3-5 parts of a penetrant, and 3-5 parts of a surfactant. The immune inducer is environmentally friendly, non-toxic and residue-free, and can effectively improve the immune resistance of plants to various plant viral diseases and reduce the disease loss.

The invention relates to a recombined insect molting hormone inactivation gene Bbsp::egt and an insecticidal fungistatic agent thereof. The recombined insect molting hormone inactivation gene Bbsp::egt contains coding beauveria bassiana chitinase signal peptide sequences (Bbsp) and coding lymantria dispar nuclepolyhedrovirus (hereinafter referred to as LdMNPV) ecdysteroid uridine 5minute-diphosphonic acid-glucosyltransferase gene (egt), and has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The molecular biological technique is adopted to shift into beauveria bassiana to obtain correct genetic transformation bacterial strains. The recombined Bbsp::egt bacterial strain obtained by the invention can disturb the normal operation of a hormone system in vivo of insects, and is beneficial to reducing immune resistance of insects on insecticidal fungi beauveria bassiana and accelerating knockout of fungus on hosts, so that the insecticidal fungi virulence is improved.

The invention relates to an intestinal motility medicinal composition, which is prepared from the following components in part by weight: 50 parts of Chinese pasque herb, 50 parts of parslane herb, 75 parts of wrinkled gianthyssop herb, 25 parts of meadowrue root and rhizome, 35 parts of medicine terminalia fruit, 25 parts of alum, 50 parts of Chinese white poplar, 30 parts of bistort rhizome and30 parts of szechuan tangshen root. The intestinal motility medicinal composition has the characteristics of obvious medicinal effect, low using cost, no toxic or side effect and the like. After the intestinal motility medicinal composition is used, the phenomena of dying of fish, shrimps and crabs are reduced obviously, and the immune resistance of organisms is improved. After the intestinal motility medicinal composition is used continuously, death caused by severe bottom environment in the middle and later periods of aquaculture can be prevented, the aquaculture yield and success rate are improved, and the economic benefits of farmers are ensured fully. Simultaneously the intestinal motility medicinal composition is a pure Chinese medicinal preparation, has no toxic or side effect and avoids medicinal residues in animal bodies, so the food quality of fish, shrimp and crab products is ensured.

The invention discloses a pet dog feed additive with a lipid metabolism adjustment function and belongs to the technical field of animal nutrition. The feed additive is prepared from, by mass, 20-30 parts of alginate oligosaccharide, 5-10 parts of carrageenan oligosaccharide, 0.5-1 part of mineral elements, 20-30 parts of seaweed meal, 20-30 parts of wheat powder, 0.5-2.5 parts of selenium yeast and 0.5-2.5 parts of calcium stearate. According to the pet dog feed additive, the ocean oligosaccharide resources are comprehensively used, through multiple mechanisms and multiple steps, lipid metabolism is adjusted, the pet dog feed additive is beneficial to improvement of lipid metabolism of the body, fat accumulation in the body is reduced, and the pet dog feed additive has high application value. According to the pet dog feed additive, alginate oligosaccharide with specific molecular weight and alginate oligosaccharide with specific molecular weight are compounded according to the specific proportion, the additive has an obvious nutrient function, the immune resistance of pet dogs can be obviously improved, the function of an immunopotentiator is achieved, and antibiotics applicationduring pet bog breeding is reduced.

The invention discloses an application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as a plant immune resistance inducer, and relates to a plant immune resistance inducer. 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid is used as a natural active substance to be developed into a plant immune resistance inducer, and the plant immune resistance inducer can be used for improving the resistance of plants to biological stress and abiotic stress and effectively preventing fungi, bacteria and viruses from infecting the plants, so that the pathogenic level is reduced; meanwhile, the tolerance of plants to high temperature, low temperature, drought and salt stress can be remarkably improved. The 2-amino-3-indolyl butyric acid or the 3-methylpyrrolidine-2-carboxylic acid has the characteristics of safety, environmental protection and high efficiency.

The invention relates to the technical field of veterinary medicine, in particular to a composition for controlling hog cholera. The composition comprises the following raw materials: winter cherry, Eucalyptus robusta leaves, Polygonum perfoliatum, atriplex centralasiatica, lycoris aurea, rhizoma anemarrhenae, pyrrosia lingua, inula herb, celandine, ardisiae japonica, cortex lycii radicis, blackberry lily, radices trichosanthis, Biebersteinia heterostemon, pegaeophyton scapiflorum, herba cepbalanoplosis segeti, corydalis boweri hemsl, elsholtzia, rehmanniachingiili, Oldenlandia diffusa and magnolia flower. The composition in the invention has an obvious control effect for hog cholera, so that the morbidity of hog cholera is controlled within 2.05%, the cure rate for hog cholera is more than 94%, the pathogenic bacteria and viruses thereof infected with hog cholera are effectively killed, and the composition has wide spectrum; moreover, the immune resistance for pigs is also improved, the stress resistance is improved, the risk of being infected with diseases is reduced, the control effect for hog cholera is effectively improved, and the composition has vitally important significance for improving pig production performance, reducing financial loss and promoting continuous and healthy development for pig breeding industry.

The invention relates to the field of aquatic feed, in particular to functional feed for improving growth and immunity of micropterus salmoides. The functional feed for improving growth and immunity of micropterus salmoides comprises fish meal, flour, soybean meal, vital gluten, beer yeast, fish oil, soya bean lecithin, monocalcium phosphate, vitamins, minerals, choline chloride and an immunopotentiator. wherein the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris. The composite immunopotentiator can improve the physique of micropterus salmoides and enhance the resistance of micropterus salmoides to pathogenic microorganisms. When the feed additive is added into feed the immunity can be obviously enhanced, and the nutritional value of cultured fishes can be improved. The use of antibiotics can be reduced or avoided, and the pollution to the environment is reduced.

The invention discloses a composite plant immune resistance inducer and a preparation method thereof. The composite plant immune resistance inducer is prepared from the following raw materials in parts by weight: 10-15 parts of alginate oligosaccharide, 18-22 parts of probenazole, 8-14 parts of lentinan, 8-19 parts of compound amino acid, 5-8 parts of compound sulfate and 1-3 parts of emulsifier.The composite plant immune resistance inducer is prepared by treating and mixing the raw materials, then concentrating by a double-effect falling-film evaporator, and performing spray drying of the concentrated mixed material. Through the synergistic effect of the reasonable components, the composite plant immune resistance inducer provided by the invention not only realizes a perfect effect of preventing and controlling wheat sharp eyespot to increase the wheat yield, but also can improve the wheat quality; meanwhile, after application, the composite plant immune resistance inducer is harmless to both human and livestock, avoids environmental pollution and creates good economic and ecological benefits.

The invention discloses a purine alkali plant immune resistance inducer and application thereof, and belongs to the technical field of biopesticides. It is found that guanine can improve the defense response of plants and reduce the morbidity and particularly has a good prevention and treatment effect on oomycete diseases of crops, and therefore guanine and structural analogues thereof can serve as effective components of the plant immune resistance inducer. The plant immune resistance inducer prepared with guanine or structural analogues thereof as effective components is mainly applied in aspraying method during application, is simple and convenient to operate, can be sprayed in advance in a high-incidence period of plants, and can effectively prevent large-area occurrence of diseases.

The invention discloses Pseudomonas corrugata and an application thereof. A strain number of Pseudomonas corrugata is S58, and a registration number is CGMCC No.17043 in the General Microbiology Center of the China Microbial Culture Collection Management Committee. The strain has stable perforamnce, high-efficiency, broad-spectrum antibacterial properties, and has the inhibitory effects for phytopathogenic fungi such as phytophthora nicotianae, phytophthora sojae, rhizoctonia solani and Magnaporthe oryzae, and acidovorax citrulli, Clavibacter, and flavimonas oryzihabitans and ralstonia solanacearum. The control effect of Pseudomonas corrugata S58 on ralstonia solanacearum is 76.2%, and the control effect against tobacco black shank is 60.5%. The Pseudomonas corrugata S58 is capable of producing ferritin and amylase, can cause programmed cell death in the surface immune response of plants, and can control the occurrence of tobacco wildfire by stimulating immune resistance. The Pseudomonas corrugata S58 is capable of hydrolyzing organic phosphorus and can be used for soil improvement.

The invention discloses a development method of a pine immune resistance inducer, and relates to the technical field of plant immune resistance inducers. The development method comprises the followingsteps: degrading and filtering soybean polypeptide powder to obtain a first raw material; dissolving 1-n-dodecyl azacycloheptane 2-ketone with absolute ethyl alcohol to obtain a second raw material;preparing fatty alcohol-polyoxyethylene ether into an aqueous solution to obtain a third raw material, adding the first raw material, the second raw material and the third raw material sequentially into water to be mixed, heating the mixed solution for 10-12 hours in a water bath at the temperature of 35-50 DEG C under the assistance of ultrasonic waves so that the pine immune resistance inducer is obtained. Soybean polypeptide powder is degraded and filtered and then mixed with the second raw material and the third raw material, the mixture conveniently penetrates through xylem of plants to be efficiently absorbed and utilized, soybean protein is used for preparing the soybean polypeptide powder, and the preparation cost is reduced; meanwhile, the plant immune resistance inducer preparedby taking the soybean polypeptide powder as a key raw material can remarkably improve the disease resistance, stress resistance and yield increase capacity of plants.

The invention discloses a plant immune resistance inducer for preventing and controlling arecanut yellow leaf disease and a use method of the plant immune resistance inducer. The plant immune resistance inducer comprises the following effective components: oxyenadenine, fucoidin, humic acid, cyantraniliprole, pyriproxyfen, a penetrant T and a surfactant, namely dodecyl betaine, wherein the fucoidin can be extracted from brown algae such as kelp, nemacystus, ascophyllum nodosum, undaria pinnatifida, sargassum fusiforme, sargassum, chorda filum and fucus vesiculosus. After the plant immune resistance inducer is diluted by 1500-2000 times, foliage spraying is performed at an early stage of arecanut yellow leaf disease; and the plant immune resistance inducer can also be sprayed in a low-concentration and small-amount manner when areca plants are not infected with the arecanut yellow leaf disease so as to realize defense. The plant immune resistance inducer aims at stimulating the immunity of plants, indirectly prevents and controls arecanut yellow leaf disease, does not enable pathogenic bacteria to generate drug resistance, is low in human and animal toxicity, good in environmental compatibility and suitable for large-scale popularization and application, controls the deterioration of the disease condition on the basis that no effective medicament is available for curing arecanut yellow leaf disease at present, and ensures the sustainable development of the betel nut industry.

The invention relates to a vaccine composition for preventing a porcine reproductive and respiratory syndrome. The vaccine composition comprises an immunizing dose of antigen of a porcine reproductive and respiratory syndrome virus strain HNjz15, high-pathogenicity porcine reproductive and respiratory syndrome virus antigen and a pharmaceutically acceptable carrier. The vaccine composition can effectively resist a new epidemic strain and the attack of high-pathogenicity porcine reproductive and respiratory syndrome viruses, produces the synergistic effect on the protection effect of the high-pathogenicity porcine reproductive and respiratory syndrome virus antigen and improves the immune resistance ability of the antigen.

The invention discloses a water quality improver for aquaculture. The improver is mainly prepared from the following raw materials: nano silver, citric acid, phytic acid, polyferric sulfate, calcium sulfate, lignin, tartaric acid, bentonite, monoammonium phosphate, amino acid, chitosan, vitamins and Chinese herbal medicine, wherein the Chinese herbal medicine includes the following Chinese herbal medicine components: radix astragali seu hedysari, herba andrographis, mulberry leaves, tangerine peel, Chinese honeylocust fruit, ginkgo leaves, codonopsis pilosula, herba taxilli, incised notopterygium rhizome and root, henon bamboo rhizome and spirulina. The water quality improver disclosed by the invention has the effects of enhancing the habitus of aquatic animals, improving the anti-stress ability and immune resistance to disease of the aquatic animals, and increasing the survival rate of the aquatic animals. A preparation method of the water quality improver disclosed by the invention is simple in process and easy to master.

The invention belongs to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for treating lung malignant tumor and an usage thereof. The traditional Chinese medicine composition comprises powder and preparation. The effective components of the powder include seaweed, semen cassiae , kelp, 10 to 20 radix angelicae dahuricae, fritillaria cirrhosa, alpinia oxyphylla, rhizoma cyperi, poria cocos, radix platycodi, fructus psoraleae and bighead atractylodes rhizome. The effective components of the preparation include clematis chinensis, angelica sinensis, prepared rhizome of rehmannia, oroxylum indicum, radix codonopsis, ligusticum wallichii, glossy privet fruit, astragalus, the root of red-rooted salvia and achyranthis bidentatae. The traditional Chinese medicine composition has the advantages that the powder and preparation are used interchangeably and drug properties are complementary to each other, and has the effects of benefiting qi and resolving blood stasis, clearing heat and removing toxicity, nourishing yin and moisturizing lung and relieving cough and reducing sputum, can inhibit the growth of tumor cells while improving the immune resistance of the organism, and has a good healing effect on lung tumor, especially for early-stage cancer and the cure rate is more than 95%. Taking the traditional Chinese medicine composition has the advantages of quick effect, short treatment course, low cost, and has no side effect.