The invention discloses a preparation of polyclonal 
antibody of heavy 
metal cadmium, and a measurement method for 
enzyme linked immunosorbent. The preparation of polyclonal 
antibody of heavy 
metal cadmium includes steps of : (A) synthesis of complete 
antigen, which is critical for preparation of the polyclonal 
antibody; (B) preparation of the antibody. The measurement method for 
enzyme linked immunosorbent includes: (1) determining optimal reaction concentration of 
coating source Cd-ITCBE-OVA and antibody by matrix 
titration, and 
coating 96-well 
enzyme label plate by 
coating source Cd-ITCBE-OVA with optimal concentration; (2) direct competitive enzyme-linked immunosorbent 
assay; (3) examining 
impact of various reaction condition on CI-ELISA, and optimizing reaction condition; (4) establishing CI-ELISA, drawing standard inhibition curve under optimized reaction condition. 
Detection limit of the invention is 0.04 mu g / L, 
linear range is 10-1-103mu g / L. The invention is specific to 
cadmium, suitable for measurement of trace cadmium in 
ambient water. The invention also provides 
technical support in fast in-situ detection for sudden accident of heavy 
metal pollution, in order to carry out remedy and 
recovery for 
pollution accident in time.