128 results about "Leymus" patented technology

The invention provides a method for directly sowing leymus chinensis seeds on a piece of soda saline-alkaline land, which comprises ridge forming, planting and irrigation management. The method comprises the following steps of: A, seed treatment; B, land preparation; C, drip irrigation zone setting; D, sowing; and E, irrigation. After the sowing of the leymus chinensis seeds, the ridges are covered by films and the land is irrigated immediately, wherein the films are 1meter wide; the amount of water used for initial irrigation is three times of that of water used for regular irrigation, and for later irrigation; and the water amount is determined according to the actual water content of soil. The ground drip irrigation and film-covering combined method for directly sowing leymus chinensis seeds improves water utilization rate, reduces water evaporation and provides good germinating and growing conditions for the leymus chinensis through the combination of leymus chinensis growing and modern irrigation technology. The method uses a soil moisture content monitoring device to monitor the water content in the post management period and irrigates according to the monitoring data so as to ensure the growth of the leymus chinensis under the condition of desirable water content and to improve the growth and tillering of the leymus chinensis. The leymus chinensis is a perennial plant and has high tillering capacity. When the coverage rate of the leymus chinensis reaches 80 percent after direct sowing, the leymus chinensis is allowed to grow naturally. The method creates remarkable long-term benefits and is a new method contributing to the development of the leymus chinensis growing industry.

The invention discloses a method to promote the rejuvenation and plant growth of leymus chinensis, which is characterized in that, during the turning green period or before the rains on the growth declination period of leymus chinensis, root cutting operation is performed to leymus chinensis grass lawn. The method has the advantages that, permeability is improved by cutting the roots, and the high yield of leymus chinensis is maintained; in addition, during the root cutting operation, the vegetative soil structure of the leymus chinensis is loosen and improved, and the soil hardening factor restricting the vegetative propagation of leymus chinensis is eliminated; therefore the permeability of the soil is improved; good environment is created for grassland vegetation growth, and the productivity of grasslands is improved; the method is suitable for the ecological restoration and management of naturally degraded leymus chinensis grass lawn; the service life of artificial leymus chinensis grass lawn is prolonged, and leymus chinensis with good quality is provided.

The invention relates to combined grass seeds which are suitable for the recovery of the black beach vegetation. The combined grass seed series are as follows: (1) the combined grass seeds are formed by blending Stipa aliena, Leymus and Puccinellia tenuiflora according to different weight proportions, and are suitable for the revegetation of the secondary bare area in all black beaches and cold and high meadow areas which takes ecological restoration as the objective; (2) the combined grass seeds are formed by blending Festuca sinensis, Poa crymophila of Qinghai and Kentucky bluegrass of Qinghai according to different weight proportions and are suitable for establishing artificial pasture, which takes grazing utilization as purpose, on the severe black beach with a slope that is less than or equal to 25 degrees; (3) the combined grass seeds are formed by blending Elymus nutans, Elymusbreviaristatus of Qinghai and Qinmu NO.1 siberian wildrye according to different weight proportions and are suitable for establishing artificial pasture, which takes grass mowing as purpose, on the severe black beach with a slope that is less than 7 degrees. The combined grass seed series of the invention have extensionality and still have space for extending the combination with the development of research and productive practice. The combined grass seed series of the invention are suitable for the revegetation of black beaches and applicable to the treatment of the black beach deteriorated grasslands with different types and grades.

An organic sheep feed for improving the quality of mutton is prepared by using the following raw materials, by weight, 100-120 parts of bur clover, 100-120 parts of Leymus chinensis, 70-80 parts of grapefruit peel, 300-320 parts of corn, 80-90 parts of corn germ meal, 5-6 parts of medicated leaven, 6-7 parts of malt, 4-5 parts of radish seed and 3-4 parts of Rangooncreeper Fruit. The addition of the grapefruit peel and the corn germ meal added to the organic sheep feed for improving the quality of mutton makes the feed have abundant nutrition and fragrant taste, and the addition of powders of medicated leaven, malt, radish seed and Rangooncreeper Fruit makes increases the daily weight increase of mutton sheep.

The invention discloses a special feed for meat donkeys and a preparation method of the special feed. The special feed comprises the following formula components in parts by mass: 25-27 parts of soybean straw, 21-23 parts of paddy rice, 14-18 parts of peas, 12-16 parts of wheat bran, 11-13 parts of rice bran, 9-12 parts of mung bean meal, 8-10 parts of carrot tops, 9-13 parts of potatoes, 6-9 parts of a cottonseed cake, 5-8 parts of bone meal, 6-9 parts of dry leymus chinensis, 7-10 parts of ryegrass, 3-5 parts of lysine, 2-6 parts of pumpkin vine powder, 1-4 parts of fennel seed powder, 2-4 parts of common salt and 50-70 parts of water. The special feed disclosed by the invention is simple in preparation, low in cost, balanced in nutrients and scientific in mixture ratio, provides various nutritional substances required by growth of the meat donkeys and enhances the disease resistance of the meat donkeys.

The invention belongs to the planting technique field, which is a chinensis germination method of combined low temperature seed soaking and temperature changing. The invention mainly comprises two parts of partially releasing the chinensis seed dormancy by the seed soaking method in the seed pretreatment technique and rapidly increasing the seed germination rate of chinensis by applying the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) low temperature seed soaking, 3) seed disinfection and 4) variable temperature germination. The invention can increase the chinensis germination rate from 56.7 percent to above 88.7 percent compared with the chinensis seed treatment by a single method. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.

The invention discloses a method for reseeding alfalfa in degenerated leymus chinensis pasture. The method comprises the following steps: reseeding alfalfa into natural pasture, and performing fertilization and pasture management of clipping at regular time, so as to maintain the ratio of the alfalfa in the natural pasture. Tests show that the pasture reseeding technique is simple to operate, and materials for reseeding, fertilizers and the like are wide in source, simple in production cost, low in cost and applicable to large-scale popularization. By adopting the reseeding technique disclosed by the invention, not only are productivity and forage grass quality of the degenerated leymus chinensis pasture improved, but also the nutrients of nitrogen in pasture soil are increased, utilization and circulation of the nitrogen in a pasture ecological system are promoted, diversity and stability of a pasture system are maintained, and technical supports are provided to establish high-yield stable natural pasture. The method has very high economic and ecological values, relatively high practicability and generalizability, and very wide application prospects.

The invention discloses a method for establishing an ecological artificial pasture on a black oil beach, which includes: firstly, selecting locally introduced and domesticated local grass-seeds; and secondly, according to the ecological and biological characteristics of forage grass and the principle of species niche in communities, mixing Elymus nutans, Leymus and Puccinellia tenuiflora according to a certain proportion, mixing Elymus nutans, Leymus, Stipa penicillata and Roegneria thoroldiana according to a certain proportion, and mixing Elymus tangutorum, Qinghai Poa pratensis, Puccinellia tenuiflora, Stipa penicillata and Roegneria thoroldiana according to a certain proportion, so that utilization rate of environmental sources is increased, the established artificial vegetation is highly stable, and the ecological benefit is evident. The method is applicable to establishment of ecological artificial pastures on deteriorated grassland of all black soil beaches with soil thickness more than 15cm.

The invention discloses a sieving method of salt tolerant cellar mutant of goat-weed, which is characterized by the following: regenerating callus induced by matured embryo; building sieving system of cellar mutant; obtaining tube vaccine; obtaining the mature goat-weed seed as explant; improving regenerating rate of matured embryo effectively.

The invention relates to a feed for cow, which belongs to the field of livestock feed. The feed provided in the invention comprises: herb residue feed prepared by using herb residue from an extraction of traditional Chinese medicine salviae miltiorrhizae and herb residue from an extraction of pseudo-ginseng as raw materials, corns, leymus chinensis, silage and commercial concentrated feed, wherein herb residue feed is prepared by processing herb residue from an extraction of traditional Chinese medicine salviae miltiorrhizae and herb residue from an extraction of pseudo-ginseng. Herb residue from an extraction of salviae miltiorrhizae can be separately processed or processed after mixing with herb residue from an extraction of pseudo-ginseng, or herb residue from an extraction of salviae miltiorrhizae and herb residue from an extraction of pseudo-ginseng are respectively processed and then mixed according to any weight ratio. The processing method is selected from an alkali treatment method, a microbial fermentation processing method, a puffed processing method or a drying processing method.

The invention relates to a leymus chinensis cutting device, in particular to an equidistant cutting device for leymus chinensis in animal husbandry. The equidistant cutting device, provided by the invention, for the leymus chinensis in the animal husbandry can cut equidistantly and is easy to operate and high in cutting efficiency. In order to solve the technical problem, the equidistant cutting device, provided by the invention, for the leymus chinensis in the animal husbandry comprises a bottom plate, a left side plate, a first sliding rail, a first pulley, a right side plate, an L-shaped rod, a guide rod, a first connecting rod, a second connecting rod, a motor, a first pawl, a lifting rod, a fixed stop block, an elastic piece, a blade, a contact wheel, a second pawl, a second spring, a pulling line, a pulling ring, a first sliding block, a first spring, a placement frame, a second sliding rail, a second sliding block, a double-sided ratchet, a second pulley, a pulling rope and a third spring. The equidistant cutting device for the leymus chinensis in the animal husbandry is driven by the motor; the double-sided ratchet can move only a short distance every time by rotating the motor anticlockwise, and the movement distances of each time are equal.

The invention provides a direct-sowing seedling culture method capable of increasing survival rate of leymus chinensis growing in moderate-severe soda saline-alkali soil and relates to a direct-sowing seedling culture method of the leymus chinensis in order to solve the problems of low germination rate, low seed emergence rate and low transplanting survival rate of the leymus chinensis in a conventional leymus chinensis direct-sowing seedling culture technology under the background of moderate-severe saline-alkali soil. The direct-sowing seedling culture method comprises steps as follows: 1, impurity removal and soaking of leymus chinensis seeds; 2, sieving of saline-alkali soil for seedling culture; 3, selection of culturing vessels; 4, filling of the culturing vessels with the saline-alkali soil; 5, trenching on the upper surface of moderate seedling culture soil and addition of isolation media; 6, sowing; 7, management after sowing. According to the direct-sowing seedling culture method, the leymus chinensis seeds can be isolated from the severe saline-alkali soil stress environment in the germination period and the acicular seedling period, a better environment is provided for growth of the leymus chinensis, and the germination rate of the leymus chinensis and the survival rate of the seedlings are increased to the great extent; the germination rate of the leymus chinensis reaches 80% or higher, and the survival rate reaches 90% or higher. The method is suitable for direct-sowing seedling culture of the leymus chinensis.

The invention relates to a Leymus chinensis aquaporin and an encoding gene and application thereof. The current researches of Leymus chinensis are only to perform eating and composition analysis for human or livestock, but the researches in molecular biology aspect, regarding Leymus chinensis as drought tolerant crop are less. The Leymus chinensis aquaporin is the protein with the following amino acid sequences: (1) the amino acid sequence in SEQ ID No:1 of the sequence table; and (2) the protein derived from the protein with the amino acid sequence in SEQ ID No:1, with the same activity of the amino acid sequence in SEQ ID No:1 of the sequence table by replacing, deleting or adding one or more of amino acid residues to amino acid sequence in SEQ ID No:1 of the sequence table. The encoding gene of the Leymus chinensis aquaporin of the invention can be transferred in other plants to increase the drought-resisting property of transgenic plants. The Leymus chinensis aquaporin and encoding gene of the invention are used in plant gene engineering field.

The invention relates to an efficient germination method of leymus chinensis seeds and aims to solve the technical problems that experiment varieties for germination of leymus chinensis seeds are limited to bred single varieties, have poorer data comparability, are time-consuming and effort-consuming and have no repeatability at present. The method comprises the steps as follows: 1, seed selection; 2, wall breakage and ultrasonic treatment; 3, preparation of a culture medium; 4, soaking with an ethanol aqueous solution and sterilization with sodium hypochlorite; 5, culture in the culture medium. The method has the advantages as follows: 1) germination of leymus chinensis can be not limited by season and environment and has the advantages of being simple, convenient, quick, efficient, seldom restricted by varieties and the like; 2) dormancy of the leymus chinensis seeds is broken through effective combination of physical, chemical and hormone for the first time, and the germination rate of the leymus chinensis is significantly increased, so that sufficient seedlings can be obtained to be applied to establishment of a leymus chinensis regeneration system and a genetic transformation system and commercial production of pasture.

The present invention discloses a Chinese wildrye levan hydrolase, and an encoded gene and an application thereof. The albumen is Chinese wildrye levan hydrolase, which is the protein having one of the following amino acid residue sequence: 1) the amino acid residue sequence of the sequence 1 in the sequence table; and protein that is obtained by substituting and / or deficiency and / or addition of one or more amino acid residue to the amino acid residue sequence of the sequence 1 in the sequence table and has [beta]-2,1 glycosidic bond hydrolase function. The encode gene of the low temperature proof albumen can be transferred to a plant to improve the stress-resistance of the plant. The gene has a significant theoretical and practical significance for the research of the stress-resistance molecular mechanism of plant and the breeding of stress-resistance varieties of plant. The gene provides an economical, fast and effective approach for improving the stress-resistance of crop. The gene has wide applications and market prospect in the agriculture field.

The invention discloses a method for restoring degraded grassland ecosystem by establishing Leymus chinensis and belongs to the field of ecological remediation. The method includes digging a round pitin an establishment site, taking 80-90% of excavated soil, adding slow-release urea, diammonium phosphate and decomposed animal waste, mixing well, and backfilling the round pit; covering the round pit with perforated degradable agricultural film, and planting pre-germinated seeds of Leymus chinensis in soil at perforated parts. The method helps prevent the problems that, for instance, shortage of moisture and nutrients causes low germination rate of Leymus chinensis seeds and low survival rate of seedlings; the method is applicable to the grassland ecosystems degraded, desertified and salinized due to various causes, and allows clonal propagation advantages of Leymus chinensis to be fully utilized so as to arrive at restoring a stretch of land by cultivating of a spot.

The invention is a chinensis germination method of combined seed peeling and temperature changing, which belongs to the planting technique field. The invention mainly comprises two parts of: partially or wholly removing the hull of chinensis seed by means of hull peeling so as to break the hard physical structure of the hull and releasing the chinensis seed dormancy aiming to destroy the hard phyical structure of the hull and break the chinensis seed dormancy; as well as rapidly increasing the seed germination rate of chinensis by using the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) hull-peeling treatment, 3) seed disinfection and 4) variable temperature germination. The invention has the increased seed germination rate from 54.0 percent to over 95.0 percent compared with the seed with hull. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.

The invention is a chinensis germination method of combined high temperature seed soaking and temperature changing, which belongs to the planting technique field. The invention mainly comprises two parts of partially releasing the chinensis seed dormancy by utilizing the seed soaking method in the seed pretreatment technique and rapidly increasing the seed germination rate of chinensis by applying the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) high temperature seed soaking, 3) seed disinfection and 4) variable temperature germination. The invention can increase the chinensis germination rate from 56.7 percent to above 84.0 percent compared with the chinensis seed treatment by a single method. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.

The invention relates to a chromosome flaking method of leymus hochst plant root tips. The method comprises the following steps that (1) in a step of preparing materials, after leymus hochst seeds are germinated for 5-7 days at constant temperature, white new roots of the potted leymussecalimus which grow vigorously are put into a centrifugal tube; (2) the centrifugal tube with root tips are placed into a high pressure container device, and N2O are introduced into the centrifugal tube for pretreatment to obtain pretreated new roots; (3) the pretreated new roots are dried and fixed by using Carnoy's fluid to obtain fixed new roots; (4) the fixed new roots are rinsed and dissociated by using glacial acetic acid after being dried to obtain dissociated new roots; (5) the dissociated new roots are dried and placed on a glass slide for manufacturing tablettings; (6) the tablettings are placed under an optical microscope for observing, dispersed and distinct cells of the chromosome are selected for taking photographs so as to perform fluorescence in situ hybridization, and finally, the positions of cover glasses are marked by using a glass knife, and the ready-made glasses are put into a refrigerator at minus 80 DEG C for storing. The method is efficient and safe.

The invention discloses a leymus chinensis accelerant and an application thereof, and belongs to the technical field of biology. The leymus chinensis accelerant provided by the invention comprises nitrogen elements, phosphorus elements and brassinolide in the mass ratio of the nitrogen elements to the phosphorus elements to the brassinolide being 10000 to (1000-2500) to (0.0001-0.0003). The leymus chinensis accelerant is used for restoring degraded leymus chinensis grasslands. The leymus chinensis accelerant disclosed by the invention can quickly increase the proportion of leymus chinensis in community by promoting tiller differentiation of the leymus chinensis, so as to restore the degraded leymus chinensis grassland.

The invention discloses a transcription factor coming from leymus chinensis and related to low temperature resistance, and a coding gene and application thereof. Protein provided by the invention is shown as the following (a) or (b): (a) protein composed of an amino acid sequence shown as sequence 1 in a sequence table; and (b) protein derived from the protein of the sequence 1, wherein the protein of the sequence 1 is subjected to substitution and / or deletion and / or adding of one or multiple amino acid residues which are related to plant cold resistance. Experiments prove that: after subjected to stress treatment at a low temperature of -8 DEG C for 24 h, at the 14th day, about 81% of wild-type arabidopis thaliana plants have the symptoms that leaves have water loss and are wilted, the color of leaves is whitened, and the plants are partially or completely dead, while about 84% of trans-LcWRKY3-genic arabidopis thaliana sprout plants grow normally, only about 16% of the plants have the symptoms that leaves are whitened; and therefore the transcription factor provided by the invention has important theoretic and practical meanings on cultivation of plant novel species with improved cold resistance, is applicable to cultivation and identification of resistance plant species required by husbandry and ecological environment treatment, and has relatively high practical application value.

The invention discloses a Leymus chinensis gamogenesis capability improving method comprising the following steps: 1, applying mixed fertilizer on a Leymus chinensis meadow in a Leymus chinensis after-fruit nutrition period, and watering the Leymus chinensis meadow so as to dissolve the fertilizer in the soil; 2, burning the Leymus chinensis in spring the next year, and maintaining soil water content at 20-35% in a Leymus chinensis turning green period; 3, spraying water solution, mixing heteroauxin with naphthalene acetic acid, on the Leymus chinensis meadow when the Leymus chinensis grows to a jointing period, and keeping spraying until the flowering stage of the Leymus chinensis; 4, spraying water solution, containing 5-amino-laevulic acid and potassium nitrate, on the flowering Leymus chinensis when the Leymus chinensis grows to the full flowering stage, and keeping spraying until the Leymus chinensis ripening state. The method couples several artificial means so as to improve Leymus chinensis heading rate, ripening rate, and seed germination capacity, thus solving the problems that conventional research can only improve one index of three low indexes of the Leymus chinensis.

The invention discloses a germination method of Leymus chinensis seeds, relating to a seed germination method, and solving the technical problems of long dormancy period and low germination rate of Leymus chinensis seeds. The method comprises the following steps of: 1, soaking shelled Leymus chinensis seeds in sterile water at 5 DEG C and in a dark condition, disinfecting the seeds with ethanol and NaClO solution, washing the seeds with sterile water, and sowing the seeds in an MS culture medium; 2, culturing for 16 hours in the dark condition and at 28 DEG C, then culturing for 8 hours at 22 DEG C, and then circulating by taking 24 hours as a cycle; and 3, germinating for 2-14 days in the conditions in the step 2 to finally obtain the germinated Leymus chinensis seeds. The method disclosed by the invention is simple and easy, simple in operation, obvious in effect, and capable of increasing the germination rate of the shelled Leymus chinensis seeds to 75.42% after low-temperature (5 DEG C) treatment from 54.47% without the low-temperature (5 DEG C) treatment.

The present invention discloses a cold resistance correlated protein from leymus chinensis, an encoding gene thereof and an application thereof. The protein provided by the invention is (a) or (b): (a) a protein with an amino acid sequence as shown in a sequence 1 in a sequence list; (b) a protein obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in the sequence 1 and related with plant cold resistance. The protein related with the plant cold resistance and the encoding gene Lc3919 of the protein provided by the invention are induced at a low temperature; a transgenic plant of the protein has relatively high cold resistance; according to the experiments, a wild type arabidopsis plant subjected to overnight cold stress at (-)8 DEG C only has a survival rate of 23.33% while the survival rate of the Lc3919 transgenic plant can be increased to 46.29% which is twice that of the survival rate of wild type arabidopsis. The Lc3919 gene can be used as a candidate gene of plant cold resistance genetic engineering for improving cold resistance of a plant.

The invention relates to the technical field of sandy land treatment and provides a method for sowing plant seeds in sand dunes to treat sandy land. The method includes: setting sand barriers at windgaps; applying base fertilizer; evenly mixing various seeds, and sowing 4-6kg of seeds into each mu of the sandy land, wherein the sown seed combination is optional one of the combination of Caraganamicrophylla, barley, Caragana korshinskii Kom. and wheatgrass, the combination of Hedysarum laeve Maxim, Artemisia desertorum, Caragana microphylla and wheatgrass, the combination of Artemisia halodendron, Achnatherum splendens, Medicago varia Martin. cv. Caoyuan No.3, Caragana microphylla, Calamagrostis epigejos, Artemisia frigida, Leymus secalinus, Leymus chinensis, Astragalus melilotoides Pall,Allium chrysanthum, Medicago sativa L. cv. Longmu 806, wild soybean, radix sileris, Elytrigia repens and Mengnong wheatgrass, and the like. By the method, plant coverage rate can reach 75-95% after the seeds sown into to the sand dunes grow into plants. The invention further provides a sandy land treatment method which comprises the method for sowing the plant seeds in the sand dunes to treat thesandy land and is capable of effectively treating the sandy land.

The invention relates to a method for rapidly constructing artificial grassland of leymus chinensis in saline-alkali degraded meadow, and aims at solving the harm of degeneration saline-alkaline spotsof existing Songnen meadow. The method for constructing the artificial grassland of leymus chinensis comprises the following steps: 1, determining the area of a degraded saline-alkali spot, adoptingsurface-layer saline-alkali spot stripping and carrying out surface-layer backfilling with aeolian sandy soil when the area of the saline-alkali spot is less than 100m<2>, and mixing a modifier into asoil layer for removing alkali for a plurality of times when the area of saline-alkali spot is greater than 100m<2>; 2, repeating a cold water immersion-draining process to break dormancy of seeds, and after air drying treatment, sowing the leymus chinensis seeds into a modified land block; and 3, carrying out routinefield management at a seedling stage of the leymus chinensis, applying a nitrogen fertilizer, and finishing the construction of the artificial grassland of leymus chinensis. Through the method for rapidly constructing artificial grassland in saline-alkali degraded meadow, the artificial grassland can be quickly constructed in the saline-alkali degraded meadow, the cover degree of vegetation is increased, the saline-alkali spot area is reduced, and the land utilization rate and the production function are improved.

The invention discloses a preparation method of a compound microorganism fermentation forage feed. The forage feed is prepared from the following components in parts by weight: 50 to 70 parts of mixed forage, 10 to 20 parts of a biological leavening agent, 5 to 15 parts of a mildew preventive and 8 to 15 parts of a degerming preservative, wherein the mixed forage consists of leymus chinensis, oat grass, legume, corn straws, sorghum stalks, wheat bran, alfalfa and bean cakes. The preparation process is simple, and the preparation process is environmentally friendly and pollution-free; the prepared forage feed is high in nutritional value; if an animal takes the feed for a long time, the immunity can be enhanced, and digestive system diseases can be reduced; meanwhile, the prepared feed can be stored for a long time, and no mildewing and rotting phenomena are caused.

The invention discloses plant low-temperature resistance related protein, its encoded gene and application. The plant low-temperature resistance related protein provided in the invention is protein of (a) or (b) as the following that: (a) is protein composed of amino acid sequences as shown in sequence 1 of a sequence table; (b) is plant low-temperature resistance related protein that is derived from (a) and is obtained by substitution and / or deletion and / or adding of one or several amino acid residues in amino acid residue sequences as shown in sequence 1 of the sequence table. Experiments prove that, LcWRKY5 has tissue expression specificity, and under low-temperature induced expression, can participate in a response of Leymus chinensis to multiple kinds of adversity stress so as to improve stress resistance of plants. LcWRKY5 and its encoded gene are of great theoretical and practical significance for cultivating low-temperature resistance improved Leymus chinensis and other new plant varieties, and can be used for cultivating and identifying resistant plant varieties needed in agriculture and animal husbandry as well as ecological environment improvement, thus boasting high practical application value.

The invention discloses a method for constructing a mixed sowing grassland by reseeding alfalfa in a slightly degraded leymus chinensis meadow, and relates to a method for constructing an artificial grassland in a degraded leymus chinensis meadow. The invention aims to improve the community structure of the leymus chinensis meadow and improve the yield and quality of forage grass. The method comprises the following steps that 1, in early July, the slightly degraded leymus chinensis meadow is cut, stubbles of 3-5 cm are reserved, and light raking operation is conducted; 2, after light raking operation, alfalfa seeds are sown, the sowing rate is 10 kg / hectare, and after sowing, a compacting machine is used for slight compacting; 3, in the current year of alfalfa sowing, the grassland is forbidden to be cut, the grassland is cut twice every year from the next year, the cutting time is selected at the initial flowering stage of alfalfa each time, and after the first cutting, the grassland is fertilized. According to the method, the yield of high-quality forage grass can be increased by 1.5-2 tons / hectare per hectare, the price of the forage grass is 1000 yuan / ton, the income can be increased by at least 1500 yuan / hectare every year, and the economic benefit in the later period is very considerable. The invention belongs to the field of degraded grassland recovery.

The invention discloses a Leymus chinensis seed germinator, which is prepared from the following raw materials by weight: 24-26 parts of biogas residue, 10-12 parts of ears of sorghum, 5-6 parts of crude wood vinegar, 3-4 parts of micro silica, 2-3 parts of silica fume, 3-4 parts of polyethylene glycol, 55-56 parts of fresh Eupatorium adenophorum, 16-17 parts of fresh oyster, 1.3-1.5 parts of Pseudomonas aeruginosa microbial inoculum, 2.2-2.4 parts of high-temperature cellulolytic decomposer microbial inoculum, a proper amount of 6mol / L HCl solution and a proper amount of water. Aiming at low germination rate of Leymus chinensis seed, polyethylene glycol dissolve the organic matter layer such as a wax layer on the seed surface, so as to improve external material exchange ability of the seed with the outside, promote seed germination, significantly improve the germination rate of Leymus chinensis seeds and greatly promote large-scale cultivation of Leymus chinensis.