116 results about "Magnetic Bead Separation" patented technology

The invention provides a kit for separating genome DNA by using magnetic balls and application thereof. The kit comprises magnetic balls, a magnetic frame, a genome DNA extraction reagent (lysing solution, binding solution, rinsing solution A, rinsing solution B and eluent) and specifications; the main steps of extracting the gene DNA comprise cell lysis, nucleic acid absorption, impurity removing and nucleic acid elution. The application of the kit for extracting the genome DNA does not need to use large-toxicity organic solvents of phenol, chloroform and the like, has good safety, and simple, fast and time-saving operation, and simultaneously can extract a plurality of samples. The kit can extract the genome DNA from materials of animals, plants, bacteria, fungus, blood, virus, animal source feed stuff, samples in the forensic medicine and the like, the DNA has high yield and purity, and the obtained genome DNA can be used for experiments such as PCR amplification, gene cloning, construction of genomic library, sequence measurement, molecular hybridization, molecular marking and the like. The kit can be stored at the temperature of 4 DEG C, also can be placed at room temperature, and is convenient to transport.

The invention discloses a magnetic bead electrochemiluminescence gene sensor-based method for detecting mercury ions and an application thereof. In the method, two probes are designed, one is a capture probe to be labelled on the surface of magnetic beads and form a magnetic bead probe; and the other probe is labelled with tris(2,2'-bipyridyl)ruthenium(II) to form an electrochemiluminescence probe. As the two probes have T:T mismatch structures, the two probes can not form a matched double helix structure at room temperature in the absence of mercury ions. When mercury ions exist, the mismatched T:T can form a stable T-Hg2 +-T coordination-complex structure with mercury ions according to the specificity of mercury ions so that mercury ions can mediate the two probes to form the double helix structure. Therefore, the magnetic bead probe can capture the electrochemiluminescence probe on the surface in the presence of mercury ions and then mercury ions can be detected through magnetic bead separation and electrochemiluminescence detection. The method of the invention has high specificity and sensitivity and facilitates the qualitative measurement and quantitative measurement of mercury ions.

The invention discloses a method for culturing natural killer (NK) and / or natural killer T (NKT) cells. The method comprises the following step: inoculating isolated NK and / or NKT cells into a culture system A for culture to obtain propagated NK and / or NKT cells, wherein the culture system A consists of a buffer solution containing CD3 antibody and / or Retronectin and inducing factors. Experiments prove that peripheral blood mononuclear cells (PBMC) extracted from peripheral blood are separated and enriched through magnetic beads, high-purity CD56+ cells are obtained, two proteins, namely Retronectin and CD3mAb are added into an in-vitro culture system for joint stimulation, and IL-2 and IL-5 factors are used for assisting in induction, so that a culture method capable of obtaining massive NK and NKT cells with high killing activity is established.

Disclosed herein is a diffusion-limiting reactor having a first element and a closure element, said reactor having at least two interconnected reservoirs said interconnection being by non-impinging microchannel, and at least one said reservoir and said microchannel being magnetic accessible. Further disclosed is a method of sample separation.

The invention provides a magnetic bead separation device for nucleic acid extraction.The magnetic bead separation device comprises a separation head lifting module, a magnetic rod module, a stirring module, an isolation sleeve separation module, isolation sleeves and reaction consumables, wherein the magnetic rod module and the stirring module are respectively in sliding connection with the separation head lifting module, the magnetic rod module comprises a plurality of magnetic rods capable of vertically moving, the stirring module comprises a plurality of gear shafts corresponding to the magnetic rods, the adjacent gear shafts are connected through gears, the core portions of the gear shafts are hollow, the lower portion of the gear shafts are used for sleeved connection with the isolation sleeves, the isolation sleeve separation module is fixed to the stirring module and is used for taking off the isolation sleeves from the gear shafts, and the reaction consumables are arranged under the gear shafts and include a splitting decomposition container, a cleaning container and an elution container.The magnetic rods are permanent magnets, and the permanent magnets are relatively small and exquisite in structure.An even liquid mixing mode of the magnetic bead separation device is a rotary-stirring even-mixing mode, the rotating speed is adjustable, and the even mixing consistency is good.

A magnetic bead-based sample separating device is provided. The device includes a first reactor, a second reactor, a third reactor, a first micro-channel and a second micro-channel. The first reactor receives a mixing solution including several magnetic beads and a sample extraction. The sample extraction is bound with the magnetic beads. The second reactor receives a washing buffer for washing the magnetic beads with sample extraction. The third reactor receives an elution buffer for separating the washing buffer from the magnetic beads. The first micro-channel is for connecting the first reactor and the second reactor and moving the magnetic beads to the second reactor from the first reactor. The second micro-channel is for connecting the second reactor and the third reactor and moving the magnetic beads to the third reactor from the second reactor.

A placenta original filled stem cell and its production are disclosed. The process is carried out by bleeding after delivering placenta from uterus, perfusing to obtain single-cell soliquoid from continuous perfusion method or collagenase digestion method and acquiring filled stem cell from gradient and density centrifugation or immune magnetic bead separation. It has better external enrichment potentiality and multidirectional differential potentiality and low immunogenicity.

The invention discloses a uniform mixing device for nucleic acid extraction. The uniform mixing device comprises a mounting rack, a driving motor, a driving gear, multiple driven gears, and a magnetic bar sleeve which corresponds to the multiple driven gears one by one, wherein a body part of the driving motor is fixed on the mounting rack; the multiple driven gears and the driving gear are pivoted on the mounting rack and are arranged along the line; the driving gear, a driven gear adjacent to the driving gear, and adjacent two driven gears are matched with one another in a meshed mode; the driving gear is synchronously coupled to a rotating shaft of the driving motor; the magnetic bar sleeve is arranged below the mounting rack, and at least one stirring blade is arranged at the outer periphery of the magnetic bar sleeve; the top of the magnetic bar sleeve is synchronously coupled to the corresponding driven gears. The liquid can be prevented from splashing from the top of a test tube, and an aerosol phenomenon is reduced, so that cross infection is effectively reduced, and the sample detection accuracy is improved. Meanwhile, the uniform mixing efficiency is high, the uniform mixing device can be matched with a magnetic bead separation device, and rapid nucleic acid separation is realized.

The invention discloses a method for screening an antithrombotic drug based on magnetic bead separation. According to the method, magnetic separation and chemical information collection (liquid chromatography-mass spectrometry) techniques are adopted; a target protein bonder is analyzed; the method is used for screening and evaluating the antithrombotic action of a drug. According to the method for screening the antithrombotic drug based on the magnetic bead separation, an optimal incubation temperature, an incubation time, a pH (potential of Hydrogen) value of a solution during incubation, ionic strength and a denaturizing cleaning solution are screened out through lots of experiments. A screening and evaluating method provided by the invention can be used for screening a large-scale natural product library or a combinatorial chemical library; in comparison with a conventional ultraviolet screening method, the screening efficiency can be obviously improved; a screening time is shortened; moreover, a synergistic effect between compounds can be discovered; the method for screening the antithrombotic drug based on the magnetic bead separation has the advantages of strong operability, quickness, high efficiency, accuracy, good repeatability and the like.

The invention provides a compound having an alpha-glucosidase inhibitory activity in lotus leaves. Nine compounds having the alpha-glucosidase inhibitory activity are screened from the lotus leaves through magnetic bead separation and liquid chromatogram-mass spectrometry analysis, and the obtained compounds are confirmed to have the alpha-glucosidase inhibitory activity through a verification of the alpha-glucosidase inhibitory activity. Compared with tradition methods, separated compounds are not needed to prepare during a primary screening, thereby substantially improving screening efficiency and shortening screening time. Due to changing a traditional activity-screening mode which separates firstly and screens secondly to a novel activity-screening mode which screens firstly and separates secondly, blindness of preparing and separating compounds is greatly reduced. The method can be popularized and applied to quick screening of compounds having the alpha-glucosidase inhibitory activity from natural medicines or herbal mixtures. The obtained compounds can be used for preparing anti-diabetic drugs.

A method for separating and isolating a target chromosome from a cellular sample by using a DNA probe with a fluorescein tag. Magnetic beads bound to anti-fluorescein isothiocyanate antibody were reacted with the fluorescently labeled pool of chromosomes and then separated in suspension by exposure to a magnetic field.

The invention provides an electrochemical sensor for detecting telomerase activity and a detection method thereof which are constructed based on functionalized nanometer magnetic beads and an exonuclease III-assisted amplification strategy, and the detection of the telomerase activity of the tumor cells is realized. In the invention, complementary probes can be hybridized with an extended telomerase primer repeated sequence, a hybrid is separated by the magnetic beads, a telomerase primer repeated sequence chain is identified and degraded by exonuclease III, a plurality of methylene blue marked hairpin DNA probes can be hybridized and opened by the released complementary probes, after the secondary degradation of the exonuclease III, the complementary probes can enter the next cycle of hybridization with an MB-marked hairpin-like structure, and the MB-marked probe is combined with a capture probe on the surface of a gold electrode, so that a peak current value which is in direct proportion to the telomerase activity is generated. According to the electrochemical sensor and the detection method thereof, the telomerase activity can be detected at a single-cell level.

The invention relates to an assessment method on the protective action of Chinese medicinal herb to the DNA oxidative damage, which comprises the steps that: DNA is fixed on the surface of a magnetic bead microsphere by covalent bonds; Chinese medicinal herb solution is added; the fixed DNA infects toxicity in vitro; magnetic bead separation is carried out to oxidize the components but reserve the oxidation-damaged DNA; 8-OH-dG antibodies are added to carry out immunoreaction with the 8-OH-dG generated by oxidative damage; enzyme-labeled second antibodies are added to react with the obtained product; and a luminous substrate is added, the high-sensitivity chemiluminescence method is adopted to detect the generated 8-OH-dG by DNA damage; the detection result is analyzed according to the detection result of the control group so as to judge whether the Chinese medicinal herb has the function of inhibiting the DNA oxidative damage to generate 8-OH-dG. The invention can detect and assess the protective action of Chinese medicinal herb to the DNA oxidative damage, and can be used for screening and developing high-efficiency and safe Chinese herbal preparations to protect the organism DNA from suffering oxidative damage.

The invention discloses a method for detecting oxytetracycline (OTC) in food by virtue of a functional magnetic bead separation-enzyme linked aptamer. The method is characterized by comprising the following steps: with a nucleic acid aptamer-magnetic nanoparticle composite probe, binding an OTC aptamer on the surface of a magnetic bead through direct competition between HRP-OTC and OTC in a sample, and detecting the change of a light absorption value through substrate chromogenic assay so as to quantitatively analyze the content of OTC in a sample. Compared with conventional enzyme-linked immuno sorbent assay (ELISA) and enzyme linked aptamer (ELAA), the method disclosed by the invention achieves the full combination of the rapid separation and enrichment effects of magnetic nano material, the high specificity of a nucleic acid aptamer and the high-throughput screening characteristic of an ELISA plate, so as to greatly shorten detection time, improve detection sensitivity and specificity and reduce detection cost.

The invention discloses an enriched enrichment and separation method of escherichia coli O157 (E.colio157), provides a basis for the follow-up study of target bacteria, and relates to the field of biotechnology. The method comprises the following steps of: carrying out covalent coupling on a broom molecule and an E.coli O157 antibody, enveloping a long chain biotin molecule by using the antibody modified broom molecule, capturing the target bacteria in a sample solution by using the broom molecule co-modified by the antibody and the long chain biotin, recognizing and coupling the long chain biotin modified broom molecule in the sample solution by a streptavidin modified nanometer magnetic bead, and subjecting the captured bacteria to separation and heavy suspension and the like, wherein a heavy suspension solution can be directly subjected to subsequent analysis. Compared with a traditional bacteria magnetic separation method, the method disclosed by the invention is more applicable to magnetically separating the bacteria in a complex matrix, and the separation efficiency of the target bacteria in the sample is improved.

The invention discloses a microfluidic-based magnetic bead separation device and method in the field of microfluidic application. An immunomagnetic bead solution inlet pipe, a bacterial mixed solution inlet pipe and a sterile water inlet pipe are integrated into a main inlet pipe, a microfluidic chip is provided with an annular pipe connected to the main inlet pipe and a main outlet pipe, a micro-pump and a sixth solenoid valve are connected to the annular pipe in series, an annular colossal magnetoresistance chip set sleeves the annular pipe, the annular pipe is provided with a groove communicated with the interior of the annular pipe and integrated to the annular pipe, a right ahead side, a right astern side and an outlet side of the groove are provided with electromagnets, an outlet of the main outlet pipe is respectively connected to a mixed solution outlet pipe and a sterile water outlet pipe, through electromagnet and solenoid valve turning-on and off, magnetic beads are gathered, an immunization magnetic bead amount is detected through a magnetoresistance chip, the inner wall of the pipe is cleaned by sterile water and simultaneously, the immunomagnetic beads are collected.

The invention discloses a quantitative detection kit for melamine. The quantitative detection kit comprises a calibration material, a quality control material, a melamine antibody solution labeled by fluorescein isothiocyanate, a melamine antibody solution labeled by alkaline phosphatase, a sample diluent, a magnetic separation reagent, cleaning fluid and a substrate solution. According to the detection method, magnetic bead separation chemiluminescence immune detection is adopted as the detection method, and an enzyme labeling technology, a magnetic separation technology and a chemiluminescence technology are combined; operation is easy and convenient, the reaction conditions are mild, the luminescence values are stable, and the outside condition influences are few; compared with an existing detection method, the magnetic bead separation chemiluminescence immune detection method has the advantages of being simple in sample processing process, low in detection cost, rapid to detect, accurate in detection result, good in repeatability and the like.

The invention relates to a magnetic bead cleaning and separating device for a full-automatic chemical luminescence immunity analyzer. The magnetic bead cleaning and separating device comprises a supporting frame, a reaction cup and an electromagnet group, wherein a reaction cup bracket is horizontally arranged on the upper part of the supporting frame; an electromagnet group bracket is mounted in the middle of the supporting frame; the reaction cup is fixed on the reaction cup bracket; a liquid absorbing needle and a liquid injecting needle are arranged above the reaction cup; the liquid absorbing needle and the liquid injecting needle are moved down and inserted into the reaction cup; the liquid injecting needle injects a reaction liquid and the liquid absorbing needle absorbs a reaction liquid in sequence, so that magnetic beads in the reaction cup are cleaned; the electromagnet group is clung to the outer wall of the reaction cup and mounted on the electromagnet group bracket; the electromagnet group is one group of electromagnets which are uniformly arranged up and down; the electromagnets independently act or simultaneously act according to a certain time sequence, and the magnetic beads in the reaction liquid are adsorbed by virtue of the electromagnetic fields produced by the electromagnets, so that mobile separation of the magnetic beads in the reaction liquid is realized. The magnetic bead cleaning and separating device is simple in structure, small in size, less in magnetic bead separation loss, and mainly suitable for non-destructive cleaning and separating of magnetic beads in medical equipment.

The invention provides a fully integrated multichannel multifunction microfluidics analysis experiment system which comprises a centralized and distributed computer control system, an air pressure preparation and adjusting module, a multichannel gas circuit control module, an integrated rapid liquid loading and propulsion control module, an image acquisition and analysis module, a magnetic needletype magnetic beads separation module and a high-accuracy three-dimensional motion platform and is characterized in that the integrated rapid liquid loading and propulsion control module comprises a plurality of gas circuit channels and a plurality of liquid medicine storage pipes, and the gas circuit channels are connected with the liquid medicine storage pipes in a one-to-one correspondence manner; the liquid medicine storage pipes and the corresponding gas circuit channels are integrated into one. The system is in a full computer control type and is efficient, reliable, novel and advanced,so that the influence of uncertain factors in an experiment process is eliminated, and the accuracy and the success probability of an experiment can be greatly improved, therefore, the system is an important auxiliary tool system for researchers and staffs engaged in the field related to microfluidics.

The invention discloses a nucleic acid extraction method and device. The device includes a sample extraction module used for holding a nucleic acid sample and extracting nucleic acid from the sample,a magnetic bead injection pump used for injecting a solution mixing micron magnetic beads into the sample extraction module, a magnetic field generation module used for generating high intensity magnetic fields, a sample cracking module used for separating the nucleic acid from the sample, and an eluent injection pump used for injecting an eluent into the sample extraction module to separate the nucleic acid from the beads so that the nucleic acid can be obtained by recycling the beads. The method and device for effectively, rapidly and semi-automatically extracting the nucleic acid in biological samples with large volume are provided; through the method, rapid extraction of the nucleic acid in samples of up to 10 ml can be realized, and the extraction time is shorter than traditional ways; and the whole extraction link can be operated automatically and combined with other detection devices later, and the purpose of fully automated detection can be achieved.

The invention discloses a copper ion detection method based on click chemistry and a detection kit. The bivalent copper ion is reduced to the cuprous ion under the effect of a reducing agent, so that the click chemistry reaction is activated; two sections of nucleotide sequences are connected with each other; a catalytic nucleic acid structure with catalytic activity can be further assembled after the magnetic bead separation; a substrate nucleic acid can be cut, so that fluorescence groups and quenching groups at the two ends of the substrate nucleic acid can be separated; and the copper ion concentration can be identified through fluorescence detection. The copper ion detection method has higher sensitivity; a detection limit to copper ions is 2pM; the detection has excellent specificity; the other conventional interfering ions have no influence on the detection; and the detection process is simple in operation, low in cost and suitable for quick detection for the copper ion concentration in practical samples.

The invention provides a method for effectively enriching air medium microorganisms on the basis of a magnetic bead method. The method comprises the following steps: placing a filter membrane in a to-be-detected environment, performing the sampling in an adsorption manner, and placing the filter membrane after the sampling into an extraction container; adding an acid buffer solution with pH value of 2.0 to 2.5, then oscillating for 5 to 10 minutes, and then carrying out the ultrasonic dispersion for 10 to 20 minutes; adding ferroferric oxide magnetic beads into the extraction container, and continuously ultrasonically dispersing for 10 to 30 seconds; taking another separation container, charging the acid buffer solution into the separation container, pouring the ultrasonically-dispersed solution into the separation container, oscillating, uniformly mixing, standing for 1 to 10 minutes, and then separating magnetic beads to obtain separated magnetic beads. By adopting the technical solution, the air medium microorganisms with high enrichment amount, high concentration and convenience in detection can be obtained on the premise of low cost, so that the problem of difficulty in tracking, enriching and quantifying in the monitoring and analysis process of air medium microorganisms can be effectively solved.

The invention discloses an efficient in-vitro amplification method of human natural killer (NK) cells. The efficient in-vitro amplification method includes the steps that CD16 monoclonal antibodies, ATG and IL-2 are added into a processed culturing system, incubation is carried out in an incubator at the temperature of 37 DEG C, counting and solution changing are carried out, and fresh IL-2 is added; a culture solution is changed at regular intervals, culturing continues for a proper time, and the human NK cells are obtained. The efficient in-vitro amplification method has the advantages that the NK cells can be efficiently amplified through the ATG and the CD16 antibodies in a short time without being subjected to separation of magnetic beads and irritation of tumor modification cells, production cost is greatly saved, and operation is easy and convenient; the number of amplification times of the NK cells is one thousand or more, and the final proportion ranges from 80% to 93%; production efficiency is high, and the efficient in-vitro amplification method can be entirely applied to large-scale clinical researching and the like; the growth process of the NK cells is completed under the GMP condition, sterile operation is guaranteed, various kinds of security detection are carried out at the final production stage, and the efficient in-vitro amplification method can be applied to clinical researching.

The invention relates to the technical field of medical equipment and particularly relates to a lung cancer circulating tumor cell detection device. The lung cancer circulating tumor cell detection device comprises a centrifuge, a fluorescent staining box, a magnetic bead separation mechanism, a display mechanism, and a machine case which is used for supporting the centrifuge, the fluorescent staining box, the magnetic bead separation mechanism and the display mechanism. According to the lung cancer circulating tumor cell detection device provided by the invention, the parts are used for detecting circulating tumor cells in blood, and the factors, which influence a diagnosis result, are few, so that a false positive medical history is not prone to occur, and furthermore, the accuracy of a detection result is effectively improved.

The invention relates to an annular porous nano magnetic bead separator which comprises a casing and magnet groups, wherein each magnet group adopts a tile shape, and the number of the magnet groups is an even number; the magnet groups are arranged in an accommodating cavity which corresponds to the shape of the magnet groups and is arranged in the casing in an annular manner; a tube hole for accommodating a tube is arranged on the casing between every two adjacent magnet groups; and the polarities of poles of every two magnet groups are contrary. The invention further relates to a mounting method of the annular porous nano magnetic bead separator. The annular porous nano magnetic bead separator and the mounting method thereof have the advantages that the utilization rate of magnets and the tube holes are effectively improved, the separation efficiency is improved, and the annular porous nano magnetic bead separator can be assembled easily. The annular porous nano magnetic bead separator belongs to the technical field of nano magnetic bead separation devices.

The invention relates to a semicarbazide derivatization reagent and a rapid detection card thereof. The derivatization reagent is aldehyde benzoic acid with a specific small molecular marker, the aldehyde benzoic acid can react with the amino of a metabolite through a aldehyde group to form a metabolite-aldehyde benzoic acid derivative with a specific molecular marker, the specific molecular marker can bind with a ligand on a magnetic bead, through magnetic bead separation, a semicarbazide derivatization product marked magnetic bead can be obtained, and the magnetic bead can be added dropwise directly on a rapid chromatographic card to perform detection. The reagent provided by the invention utilizes the advantages of lateral chromatography, simplifies the separation and purification steps of furan characteristic metabolite derivatives, and reduces the detection time and operation error.

The invention discloses a kit for quantitatively detecting a CEA (Carcino-Embryonic Antigen). The kit comprises following reagents: a biological functional magnetic nano composite particle coupled with a CEA antibody, an enzyme-labeled CEA antibody and a chemiluminiscence substrate, wherein the biological functional magnetic nano composite particle coupled with the CEA antibody is formed by compounding a nano-grade magnetic nucleus with a high molecular material containing various functional groups. According to the kit for quantitatively detecting the CEA, a nano magnetic bead separation technology, a chemiluminiscence technology and an enzyme immunoassay technology are combined together; the kit has the advantages of high sensitivity, wide linearity range, high specificity, rapidness in reaction and the like.

The invention provides a method for screening a compound with lipase inhibition activity by magnetic bead separation. The method is used for rapidly screening the compound with lipase inhibition activity rapidly from fructus aurantii by adopting a magnetic bead separation and liquid chromatography-mass spectrometry combination method. Compared with the traditional method and the prior art, the method disclosed by the invention has the advantages of being capable of rapidly screening and identifying the compound with potential lipase inhibition activity from a mixture, further separating and preparing the target compound and carrying out activity verification, thereby greatly reducing the blindness of separating effective ingredients and improving the activity screening efficiency. During screening, the compound does not need to be prepared and separated, therefore the screening efficiency is remarkably improved and the screening time is shortened. In addition, the screening method has the characteristics of high speed, accuracy, good reproducibility and the like and can be popularized to be used for rapidly screening the compound with lipase inhibition activity from natural medicines or Chinese medicine mixture. The compound with lipase inhibition activity, obtained by using the method disclosed by the invention, can be applied to preparation of diet pills and lipid-lowering medicaments.

The invention provides an automatic magnetic bead separation device. A sleeve can rotate at low speed as required by an electric device, so magnetic beads precipitated at the bottom of a test tube cansuspend in a solution, and can be fully adsorbed onto the outer wall of the sleeve under the action of a magnetic bar conveniently. The electric device can also enable the sleeve to rotate at high speed, and the generated centrifugal force can effectively spin dry other materials or solutions attached to the outer wall of the sleeve. The automatic magnetic bead separation device also realizes anautomatic telescopic functional of the magnetic bar, so the magnetic bar is ensured to be in full contact with the bottom of the sleeve, and further the attractive force on the magnetic beads at the bottom of the test tube is improved. The automatic magnetic bead separation device can effectively solve the problem in the prior art that the magnetic bead recovery rate is low and is likely to causetowing contamination. The automatic magnetic bead separation device can flexibly adapt to different sizes of sleeves, thereby meeting the requirements for simultaneous computer operation of differentspecifications of sample test tubes.