77 results about "Routine laboratory" patented technology

A cell culture apparatus consists of a vessel or array of vessels, each comprising a substantially flat bottom and substantially vertical sides, and composed of a substantially gas permeable material. The inner bottom surface of the vessel is commonly textured to provide the cells with adequate features for attachment and spreading. The vessel may include an integral annular flange which can be used to suspend the vessel from a suspensory element of a rack structure. To promote cell attachment and growth, the inner surfaces of the vessel bottom and sides may be coated with commonly available bio-active materials. Cells cultured in the system commonly grow in three dimensions for extended periods of time, and often produce significantly higher quantities of cellular products than cells grown in conventional labware.

The present invention is directed to a spectrometer in which the electrical and optical components are connected to one another in a compact construction. A minimal expenditure on assembly and adjustment is achieved through a small quantity of individual parts. The compact spectrometer comprises an entrance slit, an imaging grating, one or more detector elements in rows or matrices, and elements of a controlling and evaluating unit. The detector elements and the entrance slit are arranged on a shared support, the elements of the controlling and evaluating unit being arranged on the free surfaces of the support. The entrance slit and the detector elements and the imaging spherical grating recessed into the spectrometer housing are arranged symmetric to an imaginary center axis of the support. Due to its compact size and the minimized expenditure on adjustment and assembly for its manufacture, the spectrometer according to the invention has applications ranging from routine laboratory analysis to special tasks in process measurement technique and quality control in manufacturing processes.

The invention relates to an apparatus and method to obtain a bitumen or heavy oil sample from an oil reservoir sample, such as a core sample, to enable measurement of physical properties such as viscosity, API gravity, or chemical properties such as sulphur content of the obtained bitumen or heavy oil sample. The analyses performed on the samples obtained in accordance with the invention are effective in assisting oil field operators in making timely drilling and production decisions at the oil reservoir or for routine laboratory extraction of oils and bitumens. The invention also permits the collection of samples from simulated thermal recovery operations and also allows the collection of bitumens and oils for online analysis of live oil physical properties.

The invention relates to the field of liquid drop analysis, in particular to a micro-fluid control liquid drop generation system based on a liquid drop sequence assembly technology. In the invention, the micro-fluid control liquid drop generation system based on the liquid drop sequence assembly technology comprises an automatic liquid presenting device, a liquid drive device, a capillary and a liquid drop array plate, wherein the automatic liquid presenting device is connected with the liquid drop array plate, and the liquid drive device is connected with the capillary. The invention has the advantages that: (1) the construction of the system is simple and convenient without the complicated micro-processing technology and expensive processing equipment, so that the micro-fluid control liquid drop generation system is beneficial to wide application in a conventional laboratory; (2) the category and the mixture proportion of the liquid in the liquid drop can be optionally changed to generate liquid drops with different compositions; and (3) the generation of high flux automation of the liquid drops with the different compositions can be realized.

The invention belongs to the technical field of amino acid detection, in particular relates to a method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through a high-performance liquid chromatographic method, solving the problems that the traditional amino acid determination method has high cost and poor universality and the qualitative and quantitative analysis of the 18 varieties of standard amino acids after proteolysis cannot be quickly and accurately performed through a high-performance liquid chromatograph at present. The method comprises the steps of: pre-treating the milk powder through three different hydrolysis methods; performing pre-column derivatization of a hydrolyzed milk powder smaple through a CNBF (4-chloro-3,5-dinitrobenzotrifluoride) derivatizing agent; optimizing derivatization conditions and chromatographic fractionation detection conditions by adopting the HPLC (High-performance Liquid Chromatography) and a universal C18 (250mm*4.6mm, 5mu m) liquid chromatography column; and separating and determining all 18 varieties of proteolyzed amino acids in the milk powder. The method has the advantages of stronger universality and no need of the precious amino acid analyzer and / or the precious amino acid determination reagent kit, thereby being suitable for more common laboratories.

The invention provides a test method of the sulphate content in a flue gas desulfurization system, relating to the technical field of a test method of the sulphate content in a desulphurization system. The test method is characterized in that two samples to be tested are taken simultaneously, wherein one sample is used for determining the sulphite content, and the other sample is used for determining the total content of the sulphite and sulphate; and the sulphate content is calculated by the difference between the total content of the sulphite and sulphate and the sulphite content. The test method has the positive effects that the method is accurate, thus greatly improving the accuracy and the reliability of analysis results of the sulphate in the desulphurization system, and providing reliable analysis results for the operation of the desulphurization system. As the sulphite content in the desulphurization system is an indispensable project to be tested in the conventional analysis, thus not increasing the workload when determining the sulphate content. As the test can be completed only by using an analytical instrument in a conventional laboratory, and a determining project and a determining instrument do not need to be increased, the test method is a very practical analysis method.

A cell culture apparatus consists of a vessel or array of vessels, each comprising a substantially flat bottom and substantially vertical sides, and composed of a substantially gas permeable material. The inner bottom surface of the vessel is commonly textured to provide the cells with adequate features for attachment and spreading. The vessel may include an integral annular flange which can be used to suspend the vessel from a suspensory element of a rack structure. To promote cell attachment and growth, the inner surfaces of the vessel bottom and sides may be coated with commonly available bio-active materials. Cells cultured in the system commonly grow in three dimensions for extended periods of time, and often produce significantly higher quantities of cellular products than cells grown in conventional labware.

The invention relates to a method for determining ganoderma lucidum polysaccharide, in particular to the method for determining the content of the ganoderma lucidum polysaccharide in a glossy ganoderma product by a sulfuric acid-phenol method. The method for determining the ganoderma lucidum polysaccharide in the glossy ganoderma product by the sulfuric acid-phenol method comprises the following steps: firstly, using 80 to 85 percent of alcohol to precipitate to remove small molecular impurities such as monosaccharide, disaccharide, oligosaccharide, and so on; secondary, using distilled water to dissolve and extract polysaccharide; and finally, using the sulfuric acid-phenol method to determine the content of the ganoderma lucidum polysaccharide in the glossy ganoderma product. The method has the advantages of strong operability, accurate determination result, high sensitivity, good reproducibility, carrying out determination in general routine laboratories, and so on.

The invention relates to an instrument quality control reagent and a quality control method. Disclosed is a quality control microparticle, which is a biotin-labeled protein-coated luminescent microparticle. The present invention further provides a preparation method of the quality control microparticles and a quality control method using the quality control microparticles. The quality control microparticles and the quality control method disclosed by the invention can be used for the performance test of light-excited chemiluminescence instruments, routine laboratory quality control tests and instrument calibration.

The invention relates to a two-dimensional heat curing porous medium model, which comprises a bottom plate (2) and a cover plate (1), wherein the bottom plate (2) is formed by mixing heat reactive resin and curing agent, the cover plate (1) is also formed by mixing heat reactive resin and curing agent, the cover plate and the bottom plate are adhered with each other, the bottom plate is provided with a two-dimensional pore channel structure (3) corresponding to a pore channel structure of a rock core, and the bottom plate is further provided with a fluid inflow pore channel (4) and a fluid outflow pore channel (5) which are communicated with the two-dimensional pore channel structure. In addition, the invention further relates to a manufacturing method for the two-dimensional heat curing porous medium model. The two-dimensional heat curing porous medium model is low in technical difficulty in manufacturing, is low in requirement on hardware and easy to obtain in a conventional laboratory, the manufactured model is fine in visibility and easy in observation, and different models can be manufactured according to different master plates.

The invention provides a tissue paraffin removing liquid, which includes vegetable oil. The paraffin removing liquid not only can be used for manually removing paraffin from tissue paraffin section in a routine laboratory, and but also can be used for on-line paraffin removing of the tissue paraffin section on an automatic dyeing instrument. The tissue paraffin removing liquid can completely replace xylene during the paraffin removing process, so as to achieve excellent dyeing effect and have the paraffin removing same effect as the xylene. The natural vegetable oil as the main component of the paraffin removing liquid is harmless to human and environment.

The invention discloses a method for classifying artificial wetland obstructions and extracting components of the artificial wetland obstructions, which belongs to the technical field of sewage treatment and particularly relates to a method for classifying inter-matrix obstructions and extracting components of the inter-matrix obstructions in an artificial wetland sewage treatment technology. The artificial wetland obstructions are definitely and primarily classified into five substances, i.e., protein, polysaccharide, humic acid, nucleic acid and inorganic matter, and the like; various components of the wetland obstructions are extracted by using methods of adding strong alkali, heating and centrifuging in sequence; and the components of the obstructions are quantitatively measured in combination with a Coomassie brilliant blue method, a sulfuric acid-anthrone method, a diphenylamine method, an improved lowry method and a gravimetric method. According to the invention, the components of the obstructions are classified more comprehensively, good obstruction classification and quantitative description effect is realized, the component extracting method is simple and feasible, the components of the obstructions can be measured in most of routine test labs, and the cost required for measuring the components of the obstructions is low; the method is high in popularity, simple and easy to learn; and the control conditions can be easily guaranteed and the interference resistance is good.

The invention relates to the method of flush flow multi-point tube viscometer measuring rheological property of foam, suitable in different conditions of high temperature and high pressure, realizing the measurement in usual laboratory, uses less row material (chemical agent), needs short time, with stable performance of foam and large variation rangeof temperature, pressure and other environment parameter. Its technical proposal is: use as the main device for measuring rheological property of foam, which is formed by foam generating and stabilizing system, flush flow pipeline system, temperature control system, pressure control system and data collecting system. The experiment method is to use foam generator with unchangeable volume, measure pressure difference and temperature of the foam flowing along the pipeline in different areas and points, without measurement for foam flux.

The present invention discloses a rapid production method of a paper-based microfluidic chip, wherein a hydrophobic material solution is filled in the empty pen cartridge of conventional perfusable writing pens, drawing pens and the like, the required pattern is directly drawn on paper or is drawn by combining the pen and a template according to the actual determination demand, and different configurations of hydrophilic and hydrophobic areas are formed after drying so as to obtain the paper-based microfluidic chip capable of achieving multiple detection functions. According to the present invention, the production method has characteristics of simpleness, rapidness, low cost, good stability and the like, and the paper-based microfluidic chip is particularly suitable for being used in the conventional laboratories or underdeveloped areas, and is expected to be used for medical diagnosis, environmental monitoring, and rapid food quality detection.

A method of quantifying at least one property of interest of an oil sands ore sample is provided using a laser-induced breakdown spectroscopy (LIBS) method. The LIBS method may be applied to oil sands ore being conveyed prior to a slurry process to measure oil sand composition and provide information which may predict extraction characteristics. The LIBS method may be applied to oil sands core samples in a laboratory setting to reduce the cost and analysis time associated with conventional laboratory measurement techniques.

The invention relates to a device for hoisting a V-shaped insulator string, in particular to a V-shaped insulator string suspension device for vibration test of the V-shaped insulator string, which comprises an electric block, V-shaped insulator strings and a V-shaped insulator string assembling fitting, wherein the electric block comprises hoist cranes and a position adjusting block, the V-shaped insulator string assembling fitting is provided with two V-shaped insulator strings, one end of each V-shaped insulator string is hinged onto the V-shaped insulator string assembling fitting, the other end of each V-shaped insulator string is respectively connected with the hoist cranes, the two hoist cranes are symmetrically arranged onto an iron tower upper beam, the hoist cranes are also respectively connected with the position adjusting block, and the iron tower upper beam is parallel to the ground and is used for suspending the V-shaped insulator strings and adjusting an angle between the two insulator strings, so the defect of a suspension method of the V-shaped insulator in an ordinary laboratory can be overcome.

The invention discloses a micropore thin film micro-fluidic chip as well as a preparation method and an application thereof. The method comprises the following steps of: (1), utilizing a micropore thin film as a carrier, soaking in a photoresist standing and drying to from a photoresist thin film; and (2), enabling a mask to cover the photoresist thin film obtained in the step (1) so as to carry out exposure, finishing the exposure, dissolving the photoresist to obtain a micro-fluidic chip channel, and carrying out plasma treatment on the micro-fluidic chip channel to obtain the micropore thin film micro-fluidic chip. The chip of the method provided by the invention has the advantages that the manufacturing operation is simple and rapid, the material selection is novel and unique, the cost is low, the source is wide, and the chip is used for volume production; the operation steps can be finished in a routine laboratory, the applicability is strong, a chip manufacturing operation adopts conventional reagents such as a rare aqueous alkali, the corrosivity reagent is not used, and the environment compatibility is good.

The invention discloses a rapid microcystin extracting and detecting method comprising the following steps: firstly, conducting pretreatment on algae samples, removing impurities on microcystis flosaquae samples, cleaning the microcystis flosaquae repeatedly by distilled water, and filtering the microcystis flosaquae; secondly, conducting homogenate process and heavy weight measurement, adding algae slurry concentrated in the step one into the distilled water, stirring the slurry uniformly by a homogenizer, taking the algae slurry additionally for measuring the dry weight; thirdly, extracting toxin by methanol one-step method, calculating the measuring result of dry weight of samples in the step two, taking the algae slurry with known dry weight and volume, stirring, vibrating and mixing the algae slurry, then standing and extracting the algae slurry in a refrigerator; fourthly, conducting centrifugal purification, conducting centrifuge on extracted liquid processed in the step three, obtaining supernate, and obtaining clarified extraction liquid; fifthly, using a filter for filtering the supernate obtained from the step four; sixthly, conducting HPLC (High-performance liquid chromatography) detection, adopting optimized chromatograph condition for analyzing the toxin rapidly. The method has the advantages of high reliability, simple needed instrument, convenience in operation, high detection efficiency and the like, and is suitable for large-batch toxin extraction and analysis in a laboratory.

The invention discloses an infectivity scrapie resistance gene rapid typing detection reagent, and a preparation method and application thereof, which is characterized in that three sets of idiosyncratic primers and Taqman probes are designed and synthesized; and 136-position, 154-position, 171-position codons of sheep prion protein encoding gene PRNP which respectively determine scrapie resistance / sensibility are detected for judging whether sheep to be detected contain infectivity scrapie resistance gene. The invention establishes a rapid, simple and convenient gene typing method with strongspecificity and high sensitivity with the help of a real-time fluorescence quantitative PCR detection system and a subsequent end-point reading plate model; the detection time is only a plurality ofhours; and with the help of the method, a whole set of thorough early warning and monitoring system is established for preventing scrapie, can be applied to a conventional laboratory diagnosis method,is used as a test item for introducing live sheep by entry-exit inspection and quarantine departments so as to put an end to the introduction of the scrapie sensitivity varies.

The invention provides a method for identifying a new variety of Ziyan tea tree by SSR (simple sequences repeat) fingerprint spectrum. The method comprises the following steps of extracting total DNA(deoxyribonucleic acid) of a tea sample, using the extracted DNA as a template, using 12 pairs of SSR marker primers to respectively perform PCR (polymerase chain reaction) amplification, performing electrophoresis and dyeing on the PCR amplification product, judging whether a target strip is in a homozygosis state or a heterozygosis state, forming the fingerprint spectrum, and then identifying the Ziyan variety. The method is characterized in that 12 pairs of SSR marker primers are used for performing PCR amplification, electrophoresis and dyeing on the sample, then the strip form is observed, the homozygosis state or the heterozygosis state is judged, and the unrequired and determined Ziyan samples are simultaneously operated as above to compare. The identifying method has the advantagesthat the operation is simple and rapid, and can be completed by conventional laboratory instrument equipment; the identifying result is reliable; the important meaning is realized for the protectionand popularization of Ziyan new variety of special tea tree.

The invention provides a method for measuring the content of polysaccharide in camellia seeds. The method comprises the following steps of: firstly, sequentially extracting camellia seeds by using a low-polarity solvent and alcohol, removing liposoluble substances and soluble glycoside substances from the camellia seeds; extracting polysaccharide from the camellia seeds by using water; and finally measuring the content of the polysaccharide in the camellia seeds by adopting a sulfuric acid-phenol method. The method provided by the invention has the advantages of strong maneuverability, accuracy in measuring results and high reproducibility, can be carried out in common conventional routine test laboratories, and provides an effective guarantee for development, production and quality control of polysaccharide products.

The objective is to develop a method for determining the quality of a liquid product containing polyphenols. The present invention is a method that is a significant improvement over existing methods that use conventional laboratory instrumentation to study the quality of liquid products. The method uses an adsorption cell with a small mirror as a reflecting surface and acts as a substrate for the adsorption of the liquid's polyphenols on its surface. The polyphenol's film thickness is measured by laser ellipsometry. Light from a monochromatic light source is reflected from the thin film of polyphenol, which changes the light's optical properties and are sensed using the principles of ellipsometry. The changes in state of polarized light are translated into graphical illustrations of measured and computed parameters that can be recognized and interpreted as distinctive properties of liquid product quality.

The invention discloses a method for measuring powder hardness. The method comprises the following steps: putting powder material into a heating container, placing the heating container on a heat source, and heating until reaching the melting point of the powder material; after the powder material is completely melted, cooling to the room temperature to form a thin film; taking out the thin film and measuring the hardness of the thin film by a hardness meter. Compared with the prior art, the method has the advantages of being high in operability, accurate in measurement result and high in reproducibility, and being used for measuring in the general routine laboratory; the method provides reliable reference basis for the precise measurement of the powder. Furthermore, the method provides effective guarantee for development, production and quality control of powder products.

The invention provides a detection method and application of bull ZNF280AY (zinc finger protein 280A, Y-linked) gene copy number variations (CNVs). The method includes: taking bull genomic DNA (deoxyribonucleic acid) as a template and using PCR (polymerase chain reaction) to amplify primer pairs, wherein the PCR amplified primer pairs includes a primer pair P1 and a primer pair P2; respectively amplifying bull breed ZNF280 gene and SRY (sex-determining region, Y) reference gene through real-time quantitative PCR, and the copy number of the bull ZNF280AY gene is calculated based on quantitativeresults. The detection method based on fluorescence quantitative PCR has the advantages of high flexibility, low cost, simpleness and rapidless in operation and convenience in routine laboratory operation; large-sample detection can be achieved; in addition, the copy number variations and reproduction traits are subjected to correlation analysis so as to take the ZNF280AY gene as an effective molecular marker for selection of the reproduction traits of bulls, and the breeding effect of full reproduction is thus accelerated.

The invention belongs to the field of magnetic resonance imaging and relates to a magnetic relaxation switch-based detection method. According to the method, based on the change of transverse relaxation time T2 of magnetic nanoparticles in dispersion and aggregation states and caused gray scale change in a T2 weight image, specific target molecules are detected through functionalization modification on the surfaces of the magnetic nanoparticles. The method comprises the following steps of: mixing samples to be detected and corresponding functionalized magnetic nanoparticles; placing the mixture on a minitype detection chip; and detecting in a low field magnetic resonance analyzer. Since a minitype multi-sample detection chip with small size is used in the method, required sample quantity is small and multiple samples can be detected in parallel quickly on the low field magnetic resonance analyzer. According to the method, the problem that conventional imaging equipment has high price and large size, is difficult to operate and not suitable for routine lab application and the problem that the low field magnetic resonance analyzer has a small sample chamber and is not suitable for multi-sample parallel detection can be solved.

The invention provides a method for detecting rabbit dermatophyte and trichophyton mentagrophytes through dual quantitative PCR detection. The method comprises the steps that 1 primer is compounded; 2 genomic DNA is extracted; 3 the SYBR Green I fluorogenic quantitative PCR is conducted; 4 a SYBR Green I fluorogenic quantitative PCR melting curve is drawn; 5 result determination is conducted. The method for detecting rabbit dermatophyte and trichophyton mentagrophytes through dual quantitative PCR detection has the advantages that detecting on amplified product can be conducted in a close state, the post processing of a common PCR is leaved out, a false positive result caused by the pollution of the amplified product is avoided, the sensitivity is high, accurate quantification on beginning target gene of the reaction can be achieved further, the liner relation is good, and only 2 hours are needed to make a regular laboratory examination and send out a report.

The invention discloses a microtube distillation and condensation method and device. The method and the device can realize distillation and condensation of little liquid at the normal pressure, are in accordance with a conventional laboratory in heating and condensing condition, and meanwhile can prevent bumping of liquid. The provided microtube distillation and condensation device can be used cooperatively with an automatic distillation and condensation analyzer to realize distillation and condensation at the normal pressure. Interval bumps are arranged on the outer wall of a heating tube of the microtube distillation and condensation device, so that a heating wire can be conveniently twined for heating; through design of a gas-liquid separation ball and an olecranon-shaped gas-liquid separation tube, the gas-liquid separation is realized, and only steam can enter a condensing tube, so that the condition that liquid to be distilled is suddenly boiled to rush into a collecting tube to pollute distillation can be prevented; a characteristic concave-convex structure on the inner wall of the heating tube can be used for preventing bumping.

The invention discloses a method for measuring the emission power of an ultraviolet lamp in water. The method comprises the following steps of: placing an ultraviolet lamp arranged in a quartz sleevein water, and placing a chemical exposure meter on a perpendicular bisector of the ultraviolet lamp; recording absorbance values of the ultraviolet lamp collected by the chemical exposure meter beforeand after irradiation by controlling positions of the chemical exposure meter at different distances from the ultraviolet lamp; calculating a radiation illuminance curve of the ultraviolet lamp withthe change of distance by a system; fitting the radiation illuminance curve with a radiation illuminance curve calculated by a radiation illuminance calculation formula by the system to obtain the emission power of the ultraviolet lamp. The invention also discloses a system for measuring the emission power of the ultraviolet lamp in water. The method and the system provided by the invention can measure the actual ultraviolet emission power of the combination of the underwater ultraviolet lamp and the quartz sleeve, the cost is low, the operation method is simple and the measurement can be carried out in a conventional laboratory, so that the method and the system can be widely applied to the field of ultraviolet lamps.

The invention relates to a short-chain chlorinated paraffin online hydrogenation unit combined with a gas chromatograph and a use method thereof. The short-chain chlorinated paraffin online hydrogenation unit comprises a sample feeding part, a carrier gas supplying part and a pressure controlling part, a catalysis dechlorinating and hydrogenating reaction part which is connected with the carrier gas supplying part and provided with a heating element, a temperature control part used for performing temperature control and regulation on the catalysis dechlorinating and hydrogenating reaction part, and an interface part connected with the gas chromatograph. An online catalysis dechlorinating and hydrogenating device is connected with a commercial gas chromatograph, and is used for performing data acquisition and analysis. The short-chain chlorinated paraffin online hydrogenation unit is used for performing online catalysis dechlorinating and hydrogenating on short-chain chlorinated paraffin, and has the advantages of accuracy in quantification, simple spectrum analysis, high analysis speed, low experimental cost, less health hazard, convenience in operation, and the like. The short-chain chlorinated paraffin online hydrogenation unit is suitable for routine laboratory analysis of the short-chain chlorinated paraffin in a chlorinated paraffin product.

The invention belongs to the technical field of garden strawberry seedling propagation, and discloses a simple strawberry detoxification seedling propagation method. The method comprises the steps of 1, obtaining detoxification raw seedlings; 2, preparing a nursery garden; 3, performing repotting and transplanting; 4, promoting raw seedling stolon producing; 5, performing seedling arranging and fixing and the like. According to the simple strawberry detoxification seedling propagation method, the variety safety is effectively improved. The method is simple and convenient to implement, easy to master, high in repeatability and capable of breaking through a routine laboratory biology propagation technology, lowering labor force, reducing the material cost, and avoiding risk caused by open field breeding plant diseases and insect pests.