The invention discloses a method for realizing multi-site sequence 
molecular typing on 
haemophilus parasuis. According to the method, the operation technology of carrying out 
molecular typing on the 
haemophilus parasuis by adopting the multi-site sequence 
typing (MLST) method is disclosed for the first time. In the method, the 
genome DNA of the 
pathogenic bacteria is extracted, 7 house-keeping genes including 
rpoB, atpD, g3pd, infB, mdh, _6pgd and frdB are adopted for carrying out screening, amplification, detection and sequencing, CExpress and DNAMAN 
software is adopted for carrying out splicing, multi-
sequence alignment and analysis on the sequencing result, and then the sequence type (ST) of the strain is obtained through an on-line 
haemophilus parasuis MLST 
typing database. The amplification primers used by the 
typing technology are adopted for the first time, the PCR 
reaction system and conditions automatically optimized, and the technique process can be used for the classification identification and 
genetic diversity research of haemophilus parasuis.