116 results about "Strong anion exchange" patented technology

Purified enzymatic compositions are provided having alpha-amidating enzymes capable of catalyzing the conversion of a peptidyl compound having a C-terminal glycine residue to a corresponding peptidyl amide having an amino group in place of the C-terminal glycine. The purified compositions have specific activities above 25 mU per mg protein and are sufficiently free of proteases to allow effective catalysis of even peptidyl compounds having L-amino acids. Biologically important alpha-amidated products such as calcitonin and other regulatory hormones are efficiently produced using the alpha-amidation reaction catalyzed by the enzymes. Purification by size exclusion chromatography in combination with strong anion exchange chromatography results in homogeneous enzyme species which are used to prepare antibodies specific for the alpha-amidating enzyme. A gene capable of expressing the alpha-amidating enzyme is ligated into an expression vector and transformed into a host cell capable of expressing the gene.

An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and / or weak cation exchange binding sites in combination with one or more reverse phase and / or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.

The invention discloses a large-scale preparation method for foot-and-mouth disease totivirus marked vaccine with high yield, high purity and high safety and a product thereof. The method comprises the following steps: a)collecting a virus solution; b)performing deep filtration on a membrane, performing ultrafiltration and performing enzymolysis on nuclease; c)purifying through a strong anion exchange adsorption bed or an adsorption film; d)depositing by PEG, extracting by chloroform-isoamyl aleohl; e)inactivating; F)performing density gradient centrifugation on an inactivation liquid through cane sugar and purifying; g)performing ultrafiltration dialysis and aseptic filtration; and h)reserving a stock solution or emulsifying. The provided foot-and-mouth disease totivirus marked vaccine antigen is uniform and complete foot-and-mouth virus particle, The vaccine is injected into body, so animal infection and immunization can be completely distinguished, does not contain foot-and-mouth disease virus non-structural protein and other virus particle, and does not contain animal-based foreign protein, polypeptide and oligopeptides, animal latent anaphylactic reaction, carcinogenesis and latent risk such as mad cow disease for causing animal infectious diseases due to vaccine injection can be effectively reduced, and the vaccine has no influence on animal food safety and trade.

The invention discloses a preparation method of high-purity phycocyanin, and is characterized in that the preparation method comprises the following steps: (1) taking a fresh spirulina powder, fully mixing with a phosphate buffer solution evenly, repeatedly freezing and thawing for 7-10 times to break and remove cell walls, centrifuging to remove spirulina mud, and thus obtaining a supernatant; (2) adopting a two-step precipitation method with a 20%-30% ammonium sulfate and a 40%-60% ammonium sulfate to obtain a phycocyanin crude extract; (3) after dialyzing the crude extract, loading the sample onto a weak anion exchange column DEAE Sepharose FF, carrying out gradient elution after ion exchange, collecting an outflow component with A620 / A280 of more than 3; and (4) dialyzing the collected sample, then loading the sample onto a strong anion exchange column SOURCE30Q, carrying out gradient elution after ion exchange, collecting an outflow component with A620 / A280 of more than 4, again carrying out one-time ammonium sulfate precipitation concentration, and thus obtaining the high-purity phycocyanin having the purity of more than 4.5. The extraction purification method is simplified in process, wide in source of the raw material spirulina, simple in required equipment, and high in purity of the product, and has quite high application value.

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.

The invention provides low-molecular-weight fucosylated chondroitin sulfate, a preparation method thereof and application of the low-molecular-weight fucosylated chondroitin sulfate to the preparation of medicine for resisting Trousseau syndrome. Sulfated polysaccharide with the main chain using beta-1,4-D-glucuronic acid and beta-1,3-D-acetamino galactose as disaccharide repeating units and the branch chain being alpha-1,3-L-fucus oligosaccharide sulfate. Experiments show that the fucosylated chondroitin sulfate has an evident inhibiting effect on the Trousseau syndrome. The fucosylated chondroitin sulfate is rich in raw material source, simple in preparation process, easy in industrialization, high in product safety, good in stability, unique in activity, promising in technical prospect in the development of medicine for resisting the Trousseau syndrome, and the like.

An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and / or weak cation exchange binding sites in combination with one or more reverse phase and / or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.

The invention belongs to the field of biotechnologies, and particularly relates to a marine bacterium Cobetia sp. WG-007 screened from a waste material sample and an alginate lyase secreted from the marine bacterium and a preparation method thereof. By combining morphology and physiological and biochemical characters, the strain belongs to a Cobetia category, and is preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.7964. The strain secretes the alginate lyase, and the alginate lyase is purified by ultrafiltration and concentration, Q-Sepharose strong anion exchange chromatography, Phenyl Sepharose 6 Fast Flow hydrophobic chromatography and Superdex-G100 gel filtration technologies so as to obtain the alginate lyase with electrophoresis purity; SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) determines that the molecular weight of the alginate lyase generated by the strain is 35.0KDa.

The invention discloses an integrated proteomic sample pretreatment platform based on an SCX (strong cation exchange) / SAX (strong anion exchange) mixed filling material and application thereof. The proteomic sample pretreatment platform comprises a pipette gun head (1), strong cation exchange resin and strong anion exchange resin mixed filling materials (2) and a solid phase extraction film (3), wherein the solid phase extraction film (3) is loaded in the lower end of the pipette gun head (1); the strong cation exchange resin and strong anion exchange resin mixed filling materials (2) are loaded in the lower end of the pipette gun head (1), and are positioned above the solid phase extraction film (3). The proteomic sample pretreatment platform has the advantages that the steps of sample preenrichment, reduction, alkylation and enzymolysis are completed on the mixed ion exchange filling materials; a reactor is characterized in that the used mixed ion exchange filling materials can realize the protein enrichment at any pH values; in the enzymolysis pH replacement process, the protein loss is little; trypsinase can move back and forth between the two kinds of filling materials; the enzymolysis efficiency is higher.

The identification of the most sensitive sites of Clostridium histolyticum collagenase Class 1 to proteolysis by proteases present during the fermentation and purification of the enzyme is described. Culture supernatant obtained after fermentation of C. histolyticum is used as the starting material for further purification of the enzyme. Native collagenase Class 1 and its proteolytic fragments are partially purified by a combination of hydrophobic interaction and strong anion exchange chromatographies. The pools containing enriched levels of the proteolytic fragments are further purified by high performance anion exchange chromatography. These polypeptides are then characterized by Q-TOF mass spectroscopy. A total of three sensitive bonds are identified along with substitution and deletion strategies that will result in resistance of the enzyme to proteolytic degradation.

The present invention relates to the extraction and purification method of low molecular weight heparin for treating blood coagulation and thrombus. The coarse low molecular weight heparin material is dissolved in NaCl solution and chromatographically separated with molecular sieve gel layer to obtain the chromatographic separated liquid, and the chromatographic separated liquid is alcohol precipitated, dewatered and dried to obtain low molecular weight heparin segment. Before chromatography, the coarse product is irradiated with ultraviolet lamp or reacted with oxidant sodium hypochlorite or hydrobromic acid to eliminate harmful ¿CN-NO- radical, and treated with strong anionic exchange resin to eliminate methanol and other chemical impurity. The present invention has the low molecular weight heparin product reaching relevant international standard, and the method is simple and high in yield.

The invention discloses a method for preparing human blood coagulation factors IX and VII subcutaneously from cold-glue-removed blood plasma. The method comprises the following steps: 1, removing cold glue from blood plasma; 2, conducting strong anion-exchange column chromatography the first time; 3, conducting PEG sedimentation for removing impure protein; 4, conducting S / D viral inactivation; 5, conducting strong anion-exchange column chromatography the second time, and obtaining FVII eluent and FIX eluent; 6, conducting weak anion-exchange column chromatography, and concentration for purifying blood coagulation FVII; 7, conducting heparin affinity column chromatography for purifying blood coagulation FIX; 8, conducting ultrafiltration; 9, adding a stabilizing agent, and conducting adjustment; 10, conducting virus-removal filtration through nanofilms; 11, conducting sterilization, filtration and subpackage; 12, conducting freeze-drying; 13, conducting dry-hot viral inactivation. According to the method, PEG sedimentation is adopted for removing the impure protein, the target of preparing high-purity FVII and FIX simultaneously is achieved through combination of an ion-exchange column chromatography technology and an affinity chromatography technology, the process flow is simple, the production cycle is short, a product is subjected to three steps of virus eradicating measures, and use safety is high.

The invention relates to a protein chromatographic separation platform and application thereof. The platform comprises a proteome reactor (1) and a liquid phase chromatography-mass spectrometer (2), wherein the proteome reactor (1) comprises a pipette head (3), strong negative-ion exchange resin (4) and a solid-phase extraction membrane (5); the lower end of the pipette head (3) is filled with the solid-phase extraction membrane (5); the strong negative-ion exchange resin (4) is filled at the lower end of the pipette head (3) and is positioned on the solid-phase extraction membrane (5). The protein chromatographic separation platform provided by the invention integrates pre-concentration, reduction, alkylation, enzymolysis, strong negative ion exchange and grading of polypeptides, high pH value reverse phase grading and low pH value liquid-phase chromatography separation, has one-dimensional separation, two-dimensional separation and three-dimensional separation working modes, and can be applied to large-scale authentication and quantitative proteomics of proteins in a small amount of cells, tissues or blood samples.

The invention belongs to the technical field of synthesis and preparation methods of polypeptide drugs, and particularly relates to a synthesis and preparation method for cetrorelix acetate. The method is unique and exclusive. Rink Amide-AM resin is taken as a carrier, a low-cost HBTU / DIEA (hexafluorophosphate / diisopropylethylamine) is taken as a condensing agent, and a specific microwave synthesis technology is adopted, so that the condensation efficiency is improved and the crude yield reaches 92%; obtained crude cetrorelix acetate is purified with a reversed-phase high-performance liquid chromatography (rHPLC) method and subjected to acetate transformation treatment by adopting strong anion exchange resin, the yield of obtained pure cetrorelix acetate reaches more than 40%, and the acetic acid content of cetrorelix acetate reaches international standard requirements. The method greatly shortens reaction time and increases final product yield, thereby having considerable economic and applicable values and wide application prospects.

The invention discloses tea polyphenol oxidase isozyme monomers PP01 and PP02 and a preparation method thereof. With respect to the technical states that existing tea polyphenol oxidase is low in purification factor, low in loading quantity of protein samples and long in operation time, the obtained isozyme is the isozyme with one relative molecular weight, and the isozyme monomer with specific amino acid sequence structure, molecular weight, isoelectric point, enzymatic property and the like cannot be obtained, two tea polyphenol oxidase isozyme monomers with specific specific amino acid sequence structures, molecular weight, isoelectric points, enzymatic properties and the like are separated and prepared by sequentially adopting the technologies and optimization processes such as enrichment of zymoprotein with acetone powder, fractional precipitation of ammonium sulfate, strong anion exchange chromatography, gel medium chromatography, molecular identification and enzymatic property analysis. The method has the advantages of low cost, large preparation amount, high pertinence, short operation cycle and the like, and is of important theoretical and practical significance on deepening of the separation and purification technology of tea polyphenol oxidase isozyme, promoting of directional enzymatic synthesis of a theaflavin component by virtue of the tea polyphenol oxidase isozyme monomers, development and utilization of advantaged isozyme of tea polyphenol oxidase, isozyme immobilization, enzyme protein structure analysis, enzyme gene cloning, functional verification and the like.

The invention discloses a method for efficiently separating immunocompetence polysaccharide of ganoderma atrum. Refined ganoderma atrum polysaccharide PSG serves as raw materials of the method, strong anion exchange resin Q-Sepharose Fast Flow is adopted for separating and purifying the PSG, sodium chloride solutions in different concentrations (0-2 M) are used for performing stepwise elution, different obtained components are sequentially dialyzed, frozen and dried, and ganoderma atrume acidic beta-(1->3, 1->6)-glucan polysaccharide components with immunocompetence are obtained. The method has the advantages that different chemical components in the PSG can be effectively separated by the strong anion exchange resin; the prepared acidic polysaccharide is high in sugar content, good in uniformity and superior to refined polysaccharide PSG in immunocompetence; the method is easy to operate, fast, efficient and good in reproducibility, elution liquid in use is free of toxin and pollution, and the method can be widely used for large-scale preparation of immunocompetence polysaccharide of ganoderma atrum.

The invention provides a separation and purification method for isomers of an anthocyanin compound in lycium ruthenicum murr. Particularly, the method is to efficiently separate and purify the isomers of the anthocyanin compound from a lycium ruthenicum murr extract by adopting mixed-mode chromatography; a mixed-mode chromatographic column is an inverted-phase / strong anionic exchange mixed-mode chromatographic column; efficient separation of the anthocyanin isomers is realized under a low-pH condition by adopting the mixed-mode chromatography; a linear gradient, stepwise gradient or isocratic elution mode is used. A lycium ruthenicum murr ethyl alcohol extract is selected for inversed phase chromatographic preparation and separation to obtain 2 fractions; two pairs of cis-trans anthocyanin isomers, including a new anthocyanin and 3 anthocyanin monomer compounds separated from the lycium ruthenicum murr at the first time, are prepared through the mixed-mode chromatography. The method is excellent in separation effect on the cis-trans anthocyanin isomers, and can realize efficient separation and purification of the anthocyanin isomers and provide a substance basis for research on the activity of an anthocyanin compound.

The invention discloses a CK-MB fusion protein, a preparation method thereof, and a detection kit. The fusion protein is formed by connecting a CK-M protein and a CK-B protein through Loop. The functions of the fusion protein are the same as those of natural CK-MB protein. The preparation method comprises the following steps: coupling the coding gene sequences of human CK-M and CK-B by nucleotidebases of Loop, cloning the coupled coding gene sequences to an expression vector to obtain expression plasmids, transferring the expression plasmids to escherichia coli BL21(DE3), carrying out IPTG inducible expression, subjecting the cultured product to ultrasonic cell wall breaking, carrying out centrifugation, collecting the supernate, and finally making the supernate go through a nickel column, a strong anion exchange column Q column, and a Superdex G200 molecular sieve chromatographic column to obtain high purity protein having an active protein content of 85%. Rare earth fluorescence microspheres are taken as the labeling carrier to prepare a kit for detecting CK-MB, and the detection kit has the characteristics of stability, sensitiveness, simpleness, quantitative detection, rapidness, and reliable results.

The invention provides a method for detecting related substances in sodium ibandronate injection, and belongs to the technical field of drug analysis. According to the method for detecting the relatedsubstances in the sodium ibandronate injection, a high performance liquid chromatograph-charged aerosol detector (CAD) is adopted, a chromatographic column with octadecyl silane chemically bonded silica embedded in a strong anion exchange group as a filler is adopted, acetonitrile-water-trifluoroacetic acid serves as a flowing phase for gradient elution, the injection is subjected to Ag / H type pretreatment small column treatment, and thus the related substances in the sodium ibandronate injection can be effectively separated and determined.

The invention relates to a device for purifying biological samples and application thereof. The device comprises a strong cationic or anionic switching trap column, a connecting component and a reverse trap column and can realize simultaneous removal of strong cationic or anionic surfactant or ionic liquid and salt in biological samples. The device has the advantages of high efficiency, high recovery rate, fastness and simple operation, and can be widely used in the purification of protein samples.

The invention provides a method for separating and purifying the main sensitization protein of humulus pollen. The humulus pollen is taken as raw material and prepared into crude extract and then go through gel molecular exclusion chromatography and strong anion exchange chromatography to obtain the main sensitization protein. The protein can be combined with over 89 percent of the serological specificity IgE (sIgE) of patients allergic to the humulus pollen. The apparent molecular weight of the protein measured by SDS-PAGE is about 12kDa, the isoelectric point is 4.7 and the N-terminal sequence is NH4<+>-MDNPFENGMKA-COO<->. The separation and purification of the protein lay the foundation for realizing the standardization of the allergenic preparation of the protein.

The invention discloses a cTnI-cTnC-cTnT tripolymer protein and a preparation method thereof, and a cTnI detection kit. The tripolymer protein is formed by sequentially connecting a cTnI protein, a cTnC protein and a cTnT protein. The preparation method comprises the following steps: synthesizing a coding gene of the tripolymer protein, cloning the coding gene to an expression vector to obtain an expression plasmid, transforming the expression plasmid into Escherichia coli, and inducing the expression by using IPTG; and after the culture is finished, stirring and oscillating the culture solution with a Tris-hydrochloric acid buffer solution until bacteria are precipitated and resuspended, performing ultrasonic wall breaking, performing centrifuging, taking the supernate, and sequentially passing the supernate through a nickel column, a strong anion exchange column (Q column) and a Sephedex G200 molecular sieve chromatographic column to obtain the purified active protein. The protein can be used as a positive standard substance to prepare the time-resolved fluorescence quantitative detection kit which uses rare-earth fluorescent microspheres as the carrier. When being used for detecting cTnI in the sample, the kit is stable, sensitive, quantitative, convenient and quick, and has the characteristic of reliable results.

The invention discloses a laminar double-metal hydroxide modified capillary electrochromatography column as well as a preparation method and application thereof and belongs to the field of capillary electrochromatography columns. Laminar double-metal hydroxide has the characteristics of strong anion exchange effect, large specific surface area, porosity and the like so that the laminar double-metal hydroxide is widely applied to fields including adsorption and extraction of substances and has a good chromatographic property. A proper fixing method is lacked so that the application of the laminar double-metal hydroxide to the field of capillary electrochromatography is limited. A polydopamine modification technology is adopted so that the fixation of the laminar double-metal hydroxide on a capillary inner wall is successfully realized and the application of the laminar double-metal hydroxide to the capillary electrochromatography is realized. The preparation method of the laminar double-metal hydroxide modified capillary electrochromatography column has a simple technology and is easy to operate; the prepared capillary electrochromatography column has relatively good stability and repeatability. The laminar double-metal hydroxide modified capillary electrochromatography column prepared by the preparation method has a very good separation effect on aromatic benzene series substances and phenolic substances.

Disclosed is a method to assist polyethylene glycol(PEG) to modify hirudin with anion exchange column, belonging to protein modification technical field. The method is characterized in that ion exchange column is introduced to assit protein modification with PEG; the ph value for the reaction is 6-9 and the reaction time is 15-120min; the mol ration of hirudin to PEG is 1:3-1:9; the ion exchange column is a strong anion exchange column and the quaternary ammonium groups are the charged groups.The substrate in the column is sephadex or agarose, with average particle size of 30-90 micrometre. The method simplifies the reaction process and separation process in protein modification and correspondingly redues the consumption of sample in the process. The eluted hirudin can be recycled after being desalted. Compared with conventional liquid phase modification method, the method can produce hirudin with great activity in vitro, which is only modified by PEG.

The invention relates to a mannuronic acid and guluronic acid imbedded oligosaccharin, characterized in that the non-deacidizing end has double bonds, the preparation comprises preparing algin into water solution, charging algin cracking enzyme for reaction, heating by boiling water bath, centrifuging to remove settled foreign substance, separating and purifying through chromatography.

The invention provides a preparation method of a multifunctional mesoporous silicone solid-phase extraction agent. The preparation method of the multifunctional mesoporous silicone solid-phase extraction agent is characterized in that firstly, SBA-15 mesoporous silicone serves as a base material, 3-(2-aminoethylamino)propyl-trimethoxysilane or 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane which contains more than 2 amino groups serves a silylation reagent, multi-amino mesoporous silicone is obtained, then, ring-opening reaction of amino groups and epoxy groups is adopted, the multi-amino mesoporous silicone further reacts with excess phenyl glycidyl ether, and the mesoporous silicone extraction agent has high-density surface multifunctional groups is obtained. The mesoporous silicone extraction agent has high specific surface area of a mesoporous material, the high-density surface multifunctional groups and supramaximal extraction capacity and also has a reversed-phase adsorption effect of multiple phenyl groups and a powerful anion exchange function of quaternary ammonium groups, and therefore the mesoporous silicone extraction agent is a high-selectivity mixed-mode solid-phase extraction agent. The mesoporous silicone extraction agent is quite suitable for analyzing acid non-steroidal anti-inflammatory drugs in large-volume environmental water samples.

Hexose and pentose monosaccharides are degraded to lactic acid and glyceric acid in an aqueous solution in the presence of an excess of a strongly anionic exchange resin, such as AMBERLITE IRN78 and AMBERLITE IRA400. The glyceric acid and lactic acid can be separated from the aqueous solution. Lactic acid and glyceric acid are staple articles of commerce.

The invention provides a method for preparing a novel polyethyleneimine modified reversed-phase / strong anion exchange mixed mode adsorbent, and application of the adsorbent. The preparation method comprises the following steps: preparing a polymer with an amino functional group by adopting a Pickering emulsion polymerization method; and grafting hyperbranched polyethyleneimine to the surface of the polymer through an epoxy-amine reaction, and converting an amino group into a quaternary amino group through the epoxy-amine reaction to obtain the reversed-phase / strong anion exchange (MAX) mixed mode adsorbent with the ultrahigh capacity. According to the preparation method, the branch structure and high functional group density of polyethyleneimine are fully utilized, the preparation method is simple, and the ion exchange capacity and extraction efficiency of the material are greatly improved. The preparation method provided by the invention is simple, efficient, short in preparation period and high in yield. When used as a solid-phase extraction filler, the novel polyethyleneimine modified reversed-phase / strong anion exchange mixed mode adsorbent can be used for separating and purifying alkaline, neutral and acidic organic pollutants in a complex matrix.

The invention discloses a purification method of human immunoglobulin for intravenous injection. The purification method is used for purification of a secondary sedimentation ingredient. The purification method of the human immunoglobulin for the intravenous injection is characterized by comprising the following steps of: S1, dissolve: dissolving the secondary sedimentation ingredient with water for injection, and stirring for 2-4h at 2.0-8.0 DEG C to form a dissolve liquid, S2, filtration: filtering the dissolve liquid with a 0.45 micrometers filter membrane and then with a 0.2 micrometers filter membrane to form a filtrate, S3: filtrate adjustment: adjusting a pH (potential of hydrogen) of the filtrate to 5.60-6.00, a protein concentration to 10-13g / L, and conductivity to 0.2-1.90ms / cm to form a pre-chromatography liquid, S4, chromatography: performing chromatography with strong anion exchange gel, flushing the gel before the chromatography for balancing to allow a difference betweena pH of the gel and a pH of the liquid before the chromatography to be from -0.10 to 0.10, performing gel chromatography sample loading at a linear flow rate of 0.5-1.5cm / min and chromatography loading capacity of not exceeding 600g / L, and collecting a liquid after the chromatography. The method is simple and controllable, and greatly reduces a content of IgA (immunoglobulin A) and IgM (immunoglobulin M) in the human immunoglobulin for the intravenous injection.