The invention discloses a transparentizing 
reagent and application thereof in 
biological tissue material 
optical imaging as well as a 
living body skin tissue transparentizing imaging method. The transparentizing 
reagent is 
mannose or a mixture formed by dissoving 
mannose and 1,3-dimethyl-2-imidazolinone in a 
solvent. The transparentizing 
reagent can be applied to the 
biological tissue material 
optical imaging. The 
living body skin tissue transparentizing imaging method comprises the following steps: removing hairs on the surface, cleaning and 
drying; fixing and placing a 
skin tissue on a focalplane of an 
optical imaging system; dropwise adding the transparentizing reagent to the 
skin tissue at an imaging position, and standing for 5-30 minutes; imaging; and after the imaging is finished,wiping the transparentizing reagent, and repeatedly scrubbing and cleaning the surface of the 
skin tissue with normal 
saline or 
phosphate buffer solution, so that the 
skin tissue restores the originalstate. The reagent disclosed by the invention can not produce obvious 
tissue expansion or tissue shrinkage when in use, a detailed structure of a biological material can be completely stored, transparentizing time is short, transparentizing capability is high, and the reagent can be applied to the 
biological tissue material optical imaging.