35 results about "Trichoderma citrinoviride" patented technology

The invention discloses a microbial inoculum for preventing and curing fungal diseases of plants; the active ingredients contain Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum of the invention is used to carry out control efficiency experiments and the results show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and thecontrol efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by11.06% so that the effect for preventing diseases and increasing yield is obvious; the control efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention discloses a compound bacterial manure used for prevention and control of cotton verticillium wilt and the preparation method thereof. Materials of the compound bacterial manure consist of Streptomyces aburaviensis, Streptomyces tanashiensis, Brevibacillus agri, Bacillus clausii, Trichoderma citrinoviride, and potassium fulvic acid. Bacteria of the invention can make full use of the interspecific or intraspecific antibiosis, competition, hyperparasite, and bacteriolysis of microbes, and induce cotton to generate the disease resistance ability through secondary metabolite, thereby producing various antibiotic substances such as luteomycin and aburamycin to prevent the pathogenic bacteria of cotton verticillium wilt. The 5 strains used in the invention can be obtained through separation from soil directly; the strains can promote the growth of root, and secrete some secondary metabolites to plant roots while growing on the plant surface, inside the plant or in soil, thereby improving the plant's absorption of nutrients, and promoting the plant growth like fertilizers.

The invention relates to a lactone derivative derived from trichoderma citrinoviride and application thereof. The compound contains a molecular skeleton of hetero-oxygen atom pentacyclic lactone, and two methyls on the ring are located at the same side of the molecule. Verified by experiments, the lactone derivative has excellent anti-tumor activity. Besides, the lactone derivative can be applied to preparation of a cell proliferation inhibition drug or an anti-tumor drug and used for anti-tumor research.

The invention relates to a trichoderma citrinoviride P-T 7987 strain, which is preserved in China General Microbiological Culture Collection Center and is assigned the accession number CGMCC No.10646. The trichoderma citrinoviride P-T 7987 is strong in habitat adaptability, is high in growth speed, is high in sporulation quantity, can be used for inhibiting botrytis cinema, sclerotinia sclerotiorum, pythium aphanidermatum, phytophthora capsici and rhizoctonia solani, can be hyper-parasitized on hypha of the botrytis cinema, the sclerotinia sclerotiorum, the pythium aphanidermatum, the phytophthora capsici and the rhizoctonia solani to rapidly and effectively degrade the vegetable bacteria. The trichoderma citrinoviride P-T 7987 has a potential and developable biological prevention and treatment value.

The invention discloses a preparation method of a microbial inoculum for preventing and curing fungal diseases of plants; the microbial inoculum for preventing and curing fungal diseases of plants isprepared by mixing Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum prepared by the method of the invention is used to carry out control efficiency experiments and theresults show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and the control efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by 11.06% so that the effect for preventing diseases and increasing yield is obvious; thecontrol efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention discloses a banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and a preparation method thereof. The banana stem and leaf residue decomposing inoculant is prepared by using trametes versicolor with a cellulose, lignin and hemicellulose decomposing function, trichoderma citrinoviride, aspergillus oryzae, bacillus amyloliquefaciens with a biological control function, and streptomyces pristinaespiralis for compound fermentation. The banana stem and leaf residue decomposing inoculant can effectively accelerate the decaying of banana stem and leaf residues returned to the field, while inhibiting the growth of fusarium wilt of banana and other pathogenic bacteria; the decomposition of the banana stem and leaf residues is accelerated after applying the decomposing inoculant in the field, wherein the weight loss rate of the banana stem and leaf residues is increased remarkably; the tensile force of the banana stem and leaf residues is reduced remarkably; the number of soil pathogens including antagonistic bacillus and actinomycetes is increased remarkably; and the number of pathogenic organisms is reduced remarkably.

The present invention relates to producing micropropagules from Trichoderma citrinoviride EGE-K-130 mould strain isolated from resources of our country and whose strain is molecularly identified, as a bio-control agent by means of an eco nomical production process.

The invention discloses a trichoderma citrinoviride strain. The trichoderma citrinoviride strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the trichoderma citrinoviride strain is CGMCC No. 8722. The trichoderma citrinoviride strain has the advantages that the trichoderma citrinoviride strain dissolves pathogenic fungus cell walls through the self-produced fungal cell wall degrading enzyme, the trichoderma citrinoviride strain seeps into the cells of pathogenic fungi to form a large amount of branches and sexual structures, the growth of the pathogenic fungi is inhibited, and the pathogenic fungi die; the pathogenic fungi of pepper damping-off can be efficiently inhibited by the trichoderma citrinoviride strain, pepper damping-off is reduced, and the trichoderma citrinoviride strain can replace chemical pesticides to inhibit pepper damping-off while environmental protection is achieved.

The present invention discloses a trichoderma citrinoviride JS84 effective for promoting crop growth and a developed bio-organic fertilizer thereof. The trichoderma citrinoviride JS84 is preserved inChina general microbiological culture collection center and has a preservation number of CGMCC NO.17466. The strain is capable of antagonizing various fusarium oxysporum of bananas, cucumbers, etc. The bio-organic fertilizer is obtained by adding the functional strain JS84 to an ordinary organic fertilizer and the number of viable micro-organisms is preferably larger than or equal to 2.0x10<7> CFU / g dry weight of the organic fertilizer. The product has a good growth-promoting effect in a cucumber seedling stage, can produce high-quality seedlings with a large number of active functional microorganisms in rhizosphere, and promotes growth and yield after seedling transplanting.

The invention relates to a strain capable of producing 1,4-beta-D-xylanase and an application of the strain and can effectively solve the problem about production of 1,4-beta-D-xylanase to meet demands in industry. The method comprises steps as follows: a culture medium for enzyme production by fermentation is inoculated with the strain capable of producing 1,4-beta-D-xylanase, a fermentation broth of 1,4-beta-D-xylanase is obtained and centrifuged, ammonium sulfate is slowly dropwise added to a liquid supernatant and salted out overnight, a salting-out liquid is centrifuged, precipitates are taken and dissolved with a buffer solution, packing is performed, a buffer solution is taken as a balance solution for DAE FF gel filtration chromatography, active components are collected, packing is performed again, linear elution is performed with a balance buffer solution, active components are collected, the mass concentration of ammonium sulfate in the active components is adjusted to 40%, packing is performed, an ammonia sulfate buffer solution is taken as a balance solution for linear elution, active components are collected, salt is removed through dialysis, and dry powder of 1,4-beta-D-xylanase is obtained through freezing and suction drying. The strain capable of producing 1,4-beta-D-xylanase is a novel strain Trichoderma citrinoviride HL28-5 and has the efficacy of producing 1,4-beta-D-xylanase, and a new way of producing 1,4-beta-D-xylanase is exploited.

The invention discloses a microbial inoculum for preventing and curing fungal diseases of plants; the active ingredients contain Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum of the invention is used to carry out control efficiency experiments and the results show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and thecontrol efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by11.06% so that the effect for preventing diseases and increasing yield is obvious; the control efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention discloses trichoderma citrinoviride and application thereof in degradation of fish protein. According to the invention, Trichoderma citrinoviride 0-1 with an efficient degradation effect on fish proteins is screened from masson pine barks, and the Trichoderma citrinoviride 0-1 is preserved in the China General Microbiological Culture Collection Center on August 20, 2021, and the preservation number is CGMCC NO.23215. The Trichoderma citrinoviride 0-1 can be used for degrading fish proteins. The strain not only can efficiently degrade fish protein, but also has a better inhibition effect on cotton verticillium wilt and taro fusarium wilt, and can be developed into a novel fish protein functional fertilizer taking the strain as a functional strain.

The present invention relates to the field of microorganisms and water treatments, and discloses Trichoderma citrinoviride M-13 with the preservation number of CGMCC No.8332, wherein the microorganism can be used for reducing or absorbing Cr<6+> and treating the water body containing Cr<6+>, provides a restoration effect for the contaminated water body, and can be used for sewage treatments. According to the invention, the microorganism is used for sewage treatments, and has characteristics of simple process, high treatment efficiency, low cost and good application prospects.

The invention relates to an application of penicilliumenol E1 from trichoderma citrinoviride in preparation of anti-breast cancer drugs. The compound has the structural characteristics as follows: the compound comprises a molecular skeleton of pentacyclic amid adn a carbonyl group connected with a saturated fat long chain with ten carbon molecules, N is connected with one methyl, and one molecule contains two hydroxyl groups. Experiments prove that alkaloid compounds have better inhibition activity for breast cancer cells and can be used for anti-tumor research as a substance for preparation of drugs for inhibiting proliferation of breast cancer cells or anti-breast cancer drugs.

The invention discloses application of trichoderma citrinoviride-derived penicillium enol E1 to a liver cancer aspect. The compound has the following structure characteristics: a molecular skeleton contains one pentacyclic amide, one carbonyl is connected with one 10-carbon saturated fat long chain, N is connected with one methyl and two hydroxyl groups exist in the molecule. Experiments prove that the alkaloid compound has high inhibition activity on liver cancer cells, can be used for preparing medicines for inhibiting proliferation of the liver cancer cells or medicines for resisting liver cancer, and is applied to anti-tumor research.

The invention relates to application of penicillium enol E1 deriving from trichoderma citrinoviride in preparation of an anti-lymphoma drug. The compound is structurally characterized by containing a molecular skeleton of pentacyclic amide and a carbonyl group connected with a deca-carbon saturated fat long chain, wherein a methyl group is connected with N, and a molecule comprises two hydroxyl groups. It is verified through experiments that the alkaloid compound has good inhibitory activity on lymphoma and can be used for preparing a drug for inhibiting lymphoma cell proliferation or the anti-lymphoma drug and used in anti-tumor research.

The invention discloses an application of penilloenol E1 derived from Trichoderma citrinoviride in lung cancer. The structure of the compound is characterized in that the structure contains a pentacycloamide molecule skeleton, and a carbonyl group connected C10 saturated fat long chain, N is connected with a methyl group, and two hydroxyl groups exist in the molecule. Experiments confirm that the above alkaloid compound has good inhibition activity to lung cancer cells, can be used for preparing lung cancer cell proliferation inhibition medicines or anti-lung cancer medicines, and is used for antitumor researches.

The present invention relates to a kind of penemol E1 derived from Trichoderma aurantiacus and its application in gastric cancer. The structural characteristics of this compound are: it contains a pentacyclic amide molecular skeleton, a carbonyl group connected to a ten-carbon saturated fat long chain, a methyl group connected to N, and two hydroxyl groups in the molecule. Experiments have confirmed that the alkaloid compounds have good inhibitory activity against gastric cancer cells. It can be used to prepare gastric cancer cell proliferation inhibitory drugs or anti-gastric cancer drugs for anti-tumor research.

The invention relates to an alkaloid compound from Trichoderma citrinoviride and an application thereof. The structure of the compound is characterized in that the compound contains a molecular skeleton of pentacyclic amide and contains an unsaturated ketone connecting a 8-carbon saturated aliphatic long-chain; a methyl group is connected at N; and there are two hydroxyl groups in the molecule. It is confirmed through experiments that the alkaloid compound has good antineoplastic activity and can be used to prepare a cell proliferation inhibitor or antineoplastic drug for antineoplastic researches.

The invention discloses an application of penilloenol E1 derived from Trichoderma citrinoviride in lung cancer. The structure of the compound is characterized in that the structure contains a pentacycloamide molecule skeleton, and a carbonyl group connected C10 saturated fat long chain, N is connected with a methyl group, and two hydroxyl groups exist in the molecule. Experiments confirm that the above alkaloid compound has good inhibition activity to lung cancer cells, can be used for preparing lung cancer cell proliferation inhibition medicines or anti-lung cancer medicines, and is used for antitumor researches.

The invention relates to an application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer. The compound has the following structural characteristics: the compound contains the molecular skeleton of a pentacyclic amide, the compound contains a carbonyl group which is linked to a decane saturated fat long chain, a methyl group is linked to N, and two hydroxyl groups exist in molecules. Experiments prove that the alkaloid compound has a relatively good inhibitory activity on nasopharynx cancer; and the alkaloid compound can be used for preparing medicines for inhibiting proliferation of nasopharynx cancer cells or medicines for resisting the nasopharynx cancer, and the alkaloid compound is applicable to anti-tumor researches.

The invention relates to an alkaloid compound from Trichoderma citrinoviride and an application thereof. The structure of the compound is characterized in that the compound contains a molecular skeleton of pentacyclic amide and contains an unsaturated ketone connecting a 8-carbon saturated aliphatic long-chain; a methyl group is connected at N; and there are two hydroxyl groups in the molecule. It is confirmed through experiments that the alkaloid compound has good antineoplastic activity and can be used to prepare a cell proliferation inhibitor or antineoplastic drug for antineoplastic researches.

The invention provides a COD (chemical oxygen demand)-degrading fungicide agent and a preparation method thereof. The COD-degrading fungicide agent comprises compound fungicide liquid, a protective agent and a carrier, wherein the compound fungicide liquid comprises Aspergillus calidoustus liquid, trichoderma citrinoviride liquid, aspergillus niger liquid and rhizopus stolonifer liquid; the protective agent comprises skim milk powder, trehalose, calcium carbonate and glycerin. The preparation method comprises the following steps of (1) mixing the Aspergillus calidoustus liquid, the trichoderma citrinoviride liquid, the aspergillus niger liquid and the rhizopus stolonifer liquid into the compound fungicide liquid, adding the protective agent, and uniformly mixing; (2) crushing the carrier and treating by a 60 to 100-mesh sieve; (3) adding the crushed carrier into the compound fungicide liquid, uniformly mixing, pre-freezing, and drying, so as to obtain the fungicide agent. The fungicide agent has the advantages that the fungicide agent is used as a degradable microorganism, the strain is stable, the synergistic action is obvious, and the COD in sewage is favorably degraded.

The invention relates to penem enol E1 derived from trichoderma citrinoviride and application in preparation of an anti-oral epidermoid carcinoma drug. The compound is structurally characterized by containing a molecular skeleton of pentacyclic amide, a carbonyl connected with a long n-decyl saturated aliphatic chain, a methyl connected to N and molecules existing in two hydroxyl groups. An experiment proves that the alkaloid compound has good oral epidermoid carcinoma inhibiting activity. The penem enol E1 can serve as a drug for inhibiting oral epidermoid carcinoma cell proliferation or the anti-oral epidermoid carcinoma drug for antitumor research.

The invention relates to application of penicillium enol E1 deriving from trichoderma citrinoviride in preparation of an anti-lymphoma drug. The compound is structurally characterized by containing a molecular skeleton of pentacyclic amide and a carbonyl group connected with a deca-carbon saturated fat long chain, wherein a methyl group is connected with N, and a molecule comprises two hydroxyl groups. It is verified through experiments that the alkaloid compound has good inhibitory activity on lymphoma and can be used for preparing a drug for inhibiting lymphoma cell proliferation or the anti-lymphoma drug and used in anti-tumor research.