168 results about "Urinary protein" patented technology

Methods of detecting or predicting the onset or magnitude of kidney diseases such as acute kidney disease (AKI), previously called acute renal failure (ARF), are provided. In various aspects, methods and kits are provided to detect specific urinary proteins associated with AKI diagnosis or prognosis such as, e.g., angiotensinogen.

The invention discloses a Chinese herbal extract for treating diabetic nephropathy and a preparation method thereof. The Chinese herbal extract is prepared from 600 to 1000 parts by weight of Radix Astragali, 400 to 600 parts by weight of Gorgon fruit, 400 to 600 parts by weight of Cherokee rose-hip, 600 to 1000 parts by weight of Common achyranthes herb, 400 to 480 parts by weight of Ligustrum lucidum, 400 to 480 parts by weight of Ramulus Euonymi, and 320 to 480 parts by weight of Rheum officinale. Based on internationally unified clinical diagnostic criteria of diabetic nephropathy (DN), Compound particles of the Chinese herbal extract are carried out a comparison with Lotensin according to a randomized control principle through a contrast observation on 300 diabetic nephropathy patients taking respectively the compound particles and Lotensin, and a comparison result shows that 1) the compound particles can improve clinical symptoms of DN patients; and 2) the compound particles canreduce obviously urinary albumin in a DN early stage and urinary protein in a DN clinical stage, protect kidney functions and prevent effectively a development of early stage DN. Based on the prescribed curative effect evaluation criterion, a total effective rate of a Chinese herbal compound particle group is 86.7% and is obviously superior to that of a comparison group.

A method of assessing the ongoing kidney status of a mammal afflicted with or at risk of developing chronic renal injury or disease, including chronic renal failure (CRF) by detecting the quantity of Neutrophil Gelatinase-Associated Lipocalin (NGAL) in urine, serum or plasma samples at discrete time periods, as well as over time. Incremental increases in NGAL levels in CRF patients over a prolonged period of time are diagnostic of worsening kidney disease. This increase in NGAL precedes and correlates with other indicators of worsening chronic renal disease or CRF, such as increased serum creatinine, increased urine protein secretion, and lower glomerular filtration rate (GFR). Proper detection of worsening (or improving, if treatment has been instituted) renal status over time, confirmed by pre- and post-treatment NGAL levels in the patient, can aid the clinical practitioner in designing and / or maintaining a proper treatment regimen to slow or stop the progression of CRF.

The invention discloses a traditional Chinese medicine preparation for treating nephrosis and diabetes nephrosis proteinuria and a preparation method thereof. The preparation is prepared from hoantchy root, cinnamon, acontum fischeri, prepared radix rehmanniae, dogberry, peony bark, yam, tuckahoe and oriental waterplantain rhizome, and can be prepared into pills, granules, tablets, capsules and soft capsules. The preparation has the efficacies of nourishing Qi and raising Yang, reinforcing the spleen and kidney and clearing damp, is mainly used for treating acute and chronic nephritis, nephrosis syndrome, diabetes nephrosis oedema, long-term non-elimination of urine protein, and has definite effect.

The invention provides a method of generating and analysing a urinary protein fragmentation profile, in terms of the size, and sequence of particular fragments derived from intact filtered proteins together with the position where enzymes scission occurs along the protein polypeptide chain is characteristic of the diseased state of the kidney. With the recognition that filtered proteins are degraded during renal passage, the methods described in this application will be able to detect protein fragments derived from proteins generated by non-renal disease. The urinary analysis of these filtered proteins would currently not detect the intact form of these proteins. Therefore, a method as described to detect and analyse fragments resulting from degradation during renal passage that will be able to detect the seriousness of the disease.

The present invention is intended to develop a urine pretreatment agent, a urine pretreatment method, and a urinary protein quantitation method which reduce or cancel the influences of urine pH variations, cancel the influences of precipitates of urinary inorganic salts, and solubilize membrane proteins. The present invention provides: a urine pretreatment agent for urinary protein quantitation, comprising a buffer, a chelating agent, and a surfactant; a urine pretreatment method comprising a step of mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and a urinary protein quantitation method comprising steps of: mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and then measuring the protein concentration.

Kidney disease is diagnosed by measuring urinary catalytic iron in humans. Progressive kidney disease is treated by administering an iron chelator to humans. In particular, the progression of kidney disease essentially can be halted and the severity of kidney disease can be reduced by the administration of iron chelators to humans afflicted with a progressive kidney disease. The methods include measuring catalytic iron content in urine in a human afflicted with a progressive kidney disease and administering an iron chelator to the human. The method can include measuring total urinary protein content, blood urea nitrogen or creatinine in a blood sample before, during or after the administration of an iron chelator.

The present invention discloses a Chinese medicine for effectively curing chronic nephrosis with obvious therapeutic effect. Said Chinese medicine is made up by using 12 Chinese medicinal materials of cooked rehmannia root, Chinese yam, moutan root bark, alisma tuber, poria, hedyotis and others through a certain preparation process. Said Chinese medicine has the functions of improving renal function, regulating lipometabolism, reducing urinary protein and raising immunity of body, so that it can be effectively used for curing the diseases of chronic renal insufficiency and renal failure, etc.

The invention discloses a quantitative detection reagent and a quantitative detection method for urinary protein liquid. The reagent is obtained by mixing pyrogallol solution with an intermediate solution. The intermediate solution is obtained by dissolving a certain amount of succinic acid, sodium benzoate and sodium molybdate in distilled water. By combining an automatic analyzer with the quantitative detection reagent for the urinary protein liquid, quantitative and accurate content of protein in a urine sample can be provided, so that accurate information can be provided to clinical doctors, and quantitative, automatic and fast detection of the urine sample can be achieved.

The invention relates to a urinary protein enriching method which comprises the steps of directly adsorbing urinary proteins in urine in urinals or urinating buckets with filter cloth bags loaded with modified silica gel, and transporting the urinary protein adsorbed filter cloth bags to working points for subsequent treatment. According to the method, the isoelectric point property of specific urinary proteins is utilized, and urinary proteins such as urinary trypsin inhibitors, human urinary kininogenase, urokinase and the like are effectively absorbed directly by using the modified silica gel, macroporous resin, chitin, ionic resin and the like, so that a urine collecting step is avoided. The method is free of obvious influence on sanitary conditions of toilets, and the cost for urine transportation and a series of environmental problems resulting from urine transportation are greatly reduced.

The invention discloses an application of physically-modified Chinese cobra venom in preparation of medicaments for treating acute and chronic nephrosis. The physically-modified Chinese cobra venom is obtained by dissolving Chinese cobra venom with deionized water, heating for denaturation and then performing renaturation; the heating temperature is controlled to be 50-100 DEG C, and the heating time is controlled to be 1 min-300 min. The invention finds that the heated and denaturated Chinese cobra venom can effectively reduce urine protein in acute and chronic nephrosis, reduce serum creatinine, and urea nitrogen, improve the pathological damage degree of the kidney, and recover kidney functions.

The presently disclosed invention relates to a portable device for detecting one or more urinary proteins based on aggregation-induced emission (AIE) luminogen and quantifying the total urinary proteins in a urine sample in order for monitoring human health, in particular, to a device that utilizes a water-soluble AIE luminogen to detect one or more urinary protein aggregation in order to determine the content of both the target protein and the total urinary proteins in a subject. The device is useful at home or at clinical level for monitoring human health on regular basis.

The invention provides an application of a lectin probe combination to the urine protein glycotype-based gender identification of Qinling rhinopithecus roxellana. The lectin probe combination is a combination of PNA, VVA, BPL, AAL and PTL-I; and if a sample detection result is that PNA and VVA expression is down-regulated and BPL, AAL and PTL-I expression is up-regulated, it is showed that corresponding subjects are the female Qinling rhinopithecus roxellana, otherwise, the corresponding subjects are the male Qinling rhinopithecus roxellana. The lectin probe combination is used for identifyingthe genders of the Qinling rhinopithecus roxellana; and the lectin probe combination is rapid, simple and convenient, accurate and relatively low in implementation cost.

The present application relates to urinary protein markers for chronic pancreatitis and uses thereof. Specifically, this application relates to the use of urine protein markers obtained by using chronic pancreatitis disease models and mass spectrometry analysis in the early diagnosis of human chronic pancreatitis disease and the use of disease course monitoring. The urine protein markers are selected from Igγ‑1 chain C domain, nucleonectin‑2, beta‑2‑microglobulin, interleukin‑4 receptor alpha subunit, phosphoglycerate mutase 1, dipeptidyl peptidase 2, secreted pyrophosphoprotein 24, glycans Core protein, endothelial cell selective adhesion molecule, nestin-2, serum amyloid substance p, nerve cell adhesion molecule 1, etc. In this application, the obtained differential protein in urine provides a non-invasive and convenient option for early diagnosis and subsequent treatment of chronic pancreatitis.

The invention relates to the use of human bendazac lysine in pharmaceutical industry, wherein the bendazac lysine possesses substantial actions for reducing hypoglycemia and saccharifying hemoglobin and urine protein, thus can be applied to the prevention and treatment of diabetes.

The invention provides a traditional Chinese medicine composition for treating chronic kidney diseases and chronic renal failure. The traditional Chinese medicine composition is prepared from radix et rhizoma rhei preparata, Chinese peashrub roots, herba centellae, rhizoma curcumae, radix salviae miltiorrhizae, rhizoma smilacis glabrae, flos carthami, rhizoma polygoni cuspidati, herba pyrolae, lucid ganoderma, radix astragali and fructus aurantii. The radix et rhizoma rhei preparata has the effects of promoting blood circulation, detoxifying and eliminating turbidity, the Chinese peashrub roots has the effects of dispelling wind, detoxifying and reducring urinary protein, the herba centellae has the effects of dispelling wind, detoxifying and reducing creatinine, the rhizoma curcumae has the effects of breaking blood, promoting qi and resisting fibrosis, the radix salviae miltiorrhizae and the flos carthami have the effects of promoting blood circulation and removing blood stasis, the rhizoma smilacis glabrae has the effects of clearing heat, detoxifying and drying dampness, the rhizoma polygoni cuspidati has the effects of dispelling wind, detoxifying and reducing uric acid, the herba pyrolae has the effects of tonifying kidney and clearing away dieresis, the lucid ganoderma and the radix astragali have the effects of invigorating qi and strengthening health, and the fructus aurantii has the effects of soothing liver and regulating qi. The traditional Chinese medicine composition can effectively reduce proteinuria and hematuria of patients with chronic kidney diseases, relieve renal function progression of chronic renal failure, reduce serum creatinine, improve clinical symptoms of patients with chronic kidney diseases, especially chronic renal failure, and protect renal functions.

The invention discloses a method for preparing human albumin from urine. Resins absorbing urine protein are collected for cleaning and desorbing; elution peaks are collected for ultrafiltration and concentration; the pH is adjusted; the electric conductivity is implemented; the human albumin is prepared by the metal chelating affinity chromatography, the hydrophobic chromatography and the anion exchange chromatography. The method has the advantages of low cost, high yield, no immunogenicity and stable quality, is suitable for large-scale production operation, and develops a brand new channel for the source of the albumin.

The invention relates to the field of medical auxiliary instruments, and particularly discloses automatic urine protein detection equipment. The equipment comprises a collecting unit and a sample reserving unit. The collecting unit comprises a collecting barrel and a sealing cover, a liquid outlet is formed in the bottom of the collecting barrel, and a sampling channel is formed in the sealing cover. The sample reserving unit comprises a driving part, a rotating part, a pushing part and a plurality of sampling pipes; the plurality of sampling pipes are arranged on the rotating part along the circumferential direction of the rotating part and are detachably connected with the rotating part; a sampling rod is slidably sealed in each sampling pipe; one end of each sampling rod penetrates through one sampling pipe and is in sliding connection with the sampling pipe, the sampling rod and the sampling channel are in sliding seal, and a connecting channel is arranged in each sampling rod; a sampling spring is arranged in each sampling pipe, two ends of each sampling spring are respectively fixed on one sampling pipe and one sampling rod, and the rotating part is positioned below the sampling channels and used for pushing the sampling rods upwards. The equipment can automatically and quantitatively suck urine and store the urine, and the situation that the detection result deviates dueto the fact that the amount of the urine cannot be accurately judged manually is avoided.

The present invention is new use of mycose sulfate in pharmaceutical field. The medicine has obvious effects of obviously lowering kidney coefficient, serum inosine and urea nitrogen level, obviously increasing total serum protein and albumin, increasing urine creatinine exhaust amount, obviously decreasing exhausted urine protein, etc. It is used in treating nephritis and kidney failure disease and has the advantages of obvious curative effect, less toxic side effect, etc.

The present invention provides one kind of Chinese medicine for treating chronic kidney function failure is prepared with Chinese medicinal materials, including ginseng, astragalus root, white atractylodes rhizome, Chuanxiong rhizome, leech, etc. The medicine has high curative effect, can improve clinical symptoms, reduce urea nitrogen in blood, lower creatinine level, reduce exhausted urine protein, protect kidney function, promote the synthesis of plasma protein, raise plasma protein density, improve body's nutrient state and produce many other positive effects. It is safe clinically and low in production cost.

An adsorbent for an oral administration, comprising a surface-modified spherical activated carbon wherein an average particle diameter is 50 to 200 μm, a specific surface area determined by a BET method is 700 m2 / g or more, a bulk density is less than 0.54 g / mL, a total amount of acidic groups is 0.30 to 1.20 meq / g, and a total amount of basic groups is 0.20 to 0.9 meq / g is disclosed. The adsorbent for an oral administration has a high adsorbability, and is capable of adsorbing a large amount of toxins during a retention period in an intestine, and of remarkably reducing an amount of urinary proteins excretion.

The invention relates to a traditional Chinese medicine composite as well as a product and an application of the traditional Chinese medicine composite. The composite comprises the following traditional Chinese medicine raw materials in parts by weight: 20-40 parts of Astragalus mongholicus and 10-20 parts of mulberry and also comprises one or several of the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of salviae miltiorrhizae, 5-15 parts of angelica sinensis, 10-20 parts of processed fleece-flower root, 5-15 parts of rhizoma chuanxiong and 1-5 parts of pseudo-ginseng. The traditional Chinese medicine composite has better actions on lowering sugar, lowering fat, improving a blood rheology index, reducing urine protein or improving the creatinine clearance rate and especially has the actions on protecting the vascular endothelial cell, alleviating the damage degree of the endarterium and the like, and therefore the traditional Chinese medicine composite can be prepared into oral administration or external application products used for treating or controlling renal damage, neuropathy around limbs, high blood fat, impotence, prostatic hyperplasia, diabetes mellitus and complications, such as diabetic foot, diabetic nephropathy and diabetic retinal inflammation, and has good protecting action on the liver, the kidney, the pancreas and the intestinal mucosa.

The invention relates to a sampler, in particular to an interval type urine sampler for urine protein detection in the cardiovascular medicine department. The technical problem is to provide the interval type urine sampler for urine protein detection in the cardiovascular medicine department, which is convenient for people to operate and can prevent urine from splashing onto hands. The interval type urine sampler for urine protein detection in the cardiovascular medicine department comprises a base and a first fixing support, wherein the first fixing support is arranged on one side of the topof the base; a storage barrel arranged in the middle of the first fixing support; a feeding funnel arranged at the lower part of one side of the storage barrel; and a cover plate rotationally arrangedon one side of the top of the feeding funnel. Through the cooperation of a stirring mechanism and a transmission mechanism, the urine of people can be automatically sampled, and the use by people isfacilitated; by means of the transmission mechanism, people can conveniently collect and sample urine, people do not need to manually receive the urine, and the urine is prevented from splashing to the hands.

Based on detection of Treponema bacteria or Campylobacter bacteria present in a sample derived from the tonsil of a subject who has a positive result on at least one of a urinary protein test and a urinary occult blood test, or of a patient diagnosed with possible IgA nephropathy, presumed IgA nephropathy can be detected with a high accuracy, in a simple and quick manner without physical burden on subjects. Also, an IgA nephropathy patient for whom tonsillectomy is effective in therapy of IgA nephropathy can be selected with a high accuracy, in a simple and quick manner without physical burden on subjects.

The invention discloses a formula of a traditional Chinese medicine for treating diabetic nephropathy. The formula is characterized by involving the following raw materials by weight: 6 parts of astragalus roots, 3 parts of Pseudostellaria heterophylla, 2 parts of angelica, 2 parts of radix rehmanniae, 2 parts of cornus officinalis, 2 parts of Salvia miltiorrhiza, 3 parts of raw coix seeds, 2 parts of earthworm, 2 parts of Poria, and 2 parts of rhizoma alismatis. Directed at qi-yin deficiency, i.e. the traditional Chinese medicinal core pathogenesis of diabetic nephropathy, the formula has the efficacy of supplementing Qi and nourishing Yin, dispersing blood stasis and promoting dieresis, and is suitable for different-stage diabetic nephropathy with the main manifestations of soreness and weakness of waist and knees, weakness all over the body, edema, foaming urine, nausea, skin pruritus, limb numbness or acroanesthesia (or chilling), dark red tongue, thin or greasy tongue coat, thin pulse or rapid pulse and the like. The formula involves simple medicines and can achieve a special effect. Previous long-term clinical practice and scientific research prove that the formula can significantly reduce the urinary protein excretion rate, delay the renal function progress, effectively improve glucolipid metabolism, effectively control blood pressure, improve the quality of life of patients, and greatly decrease kidney disease end point events, and has the advantages of stable long-term effect and good safety.

Disclosed is a renal disease detection method which involves a complex detection step of detecting a complex of an antigen derived from a human renal mesangial cell and human IgA in a sample derived from urine collected from a subject. It is preferred that the renal disease detection method further involves a determination step of determining the renal disease as IgA nephropathy based on the ratio of the quantity of the complex detected in the complex detection step to the quantity of a urinary protein in the sample. The renal disease detection method has good detection sensitivity and specificity, and can determine the occurrence of a renal disease (preferably, IgA nephropathy) conveniently and safely.

The invention relates to the technical field of medicine. The clinical manifestations of glomerulonephritis are proteinuria, hematuria, edema, hypertension, etc. Among them, chronic glomerulonephritis has a long course of disease, often has different degrees of renal function damage, and has recurrent attacks, and the prognosis is poor. The purpose of the present invention is to provide the application of Clary sage extract in the preparation of medicine for treating glomerulonephritis. The invention provides an extract of sage ganxie obtained by drying and pulverizing medicinal materials of the roots of sage ganxie, soaking them in water-containing lower alcohol, extracting by percolation or heating, and concentrating and drying the extract. The above-mentioned Clary Sage extract has been confirmed by animal experiments to significantly reduce the urinary protein content of serum sickness glomerulonephritis model rats, and can significantly reduce the swelling of the glomerulus, and also has the ability to reduce blood viscosity and diuresis. therapeutic effect. The invention not only provides a new application for the ganxi sage, but also provides a new treatment drug for the majority of nephritis patients.

The invention belongs to the technical field of nephropathy prediction, and discloses a hypertensive nephropathy prediction system and method based on an incremental neural network model. The hypertensive nephropathy prediction system based on the incremental neural network model comprises a kidney image acquisition module, a blood pressure acquisition module, a urine protein acquisition module, amain control module, an image enhancement module, an image feature extraction module, an index comparison module, a disease analysis module, a disease prediction module, a screening module, a nephropathy supervision module, a cloud storage module and a display module. According to the invention, through the screening module, the screening efficiency of the hypertensive nephropathy is improved; and through the nephropathy supervision module, a patient can be treated according to a hypertensive nephropathy management scheme suitable for himself / herself, potential hypertensive nephropathy patients can be screened out as early as possible, and a reasonable management scheme is provided for the potential hypertensive nephropathy patients in time, so that the requirements for reducing the increasing speed of the number of the hypertensive nephropathy patients and relieving the burden of medical insurance funds are met.

The invention relates to use of cordyceps cicadae in preparation of drugs for preventing or treating gout. According to the use, due to cordyceps cicada preparation products, a novel gout preventing or treating method is provided and is obvious in effect. The cordyceps cicadae have the functions of reducing blood and urine creatinine, increasing endogenous creatinine clearance rate, increasing the content of serum protein, reducing the discharge of urinary protein, and the like. Therefore, the cordyceps cicadae have definite treatment effect on early- and middle-stage chronic renal insufficiency patients. Confirmed by further researches: the cordyceps cicadae have relatively good treatment effect on renal tubulointerstitial lesions and can be used for protecting Na<+>-K<+>-ATPase of renal tubule cells, alleviating cytolysosome and cell lipid peroxidation injury, improving renal hemodynamics and alleviating endothelial cell injury and blood coagulation property.