A method of complex frequency-domain 
optical coherence tomography using differential sinusoidal 
phase modulation includes: based on complex frequency-domain 
optical coherence tomography using differential sinusoidal 
phase modulation, introducing sinusoidal 
phase modulation to transverse scan interference signals, subjecting acquired interference spectral signals to inverse 
Fourier transform along the wave-number direction, using a 
phase difference of adjacent complex tomographic signals to replace phases of complex tomographic signals obtained in conversion so that new differential complex tomographic signals are formed, respectively subjecting a real part and an imagery part of each differential complex tomographic 
signal to phase 
demodulation prior to addition so as to obtain mirror-removed complex tomographic signals, and removing amplitude to obtain a full-depth structural tomographic map of a tested sample. By the method, complex conjugate mirror images, 
direct current background and autocorrelation 
noise in the frequency-domain optical coherence tomographic images are eliminated, sensitivity does not decrease with increase of transverse scanning distance, the affection of internal high-speed movement of a sample on 
mirror image elimination is reduced, and the method is applicable to 
in vivo imaging of biological samples.