160results about How to "Accelerate cultivation" patented technology

The present invention relates to the use of interferon in the in vitro cultivation of animal circular ssDNA virus such as Porcine Circovirus 2 or human TT virus in an animal cell line. Increased titres of animal circular ssDNA virus are obtained by addition of interferons or agents which ensure the production of endogenous interferons by said cell line.

The present invention describes a new substrate comprising olive oil waste as the nutrient for growing edible and medicinal shiitake mushrooms (Lentinus edodes) in aseptic plastic bags. The invention further provides the strain Ile-1, a new and distinct variety of the species Lentinus edodes, that grows very efficiently in the olive oil waste-containing substrate.

This invention is directed to a method of growing a plant from the Cannabaceae family, the cultivar or composition produced therefrom, wherein the plant is exposed to artificial lighting of different intensities based on spacing, growth phase, and flowering yield.

The invention provides a method for detecting the off-target effect of an adenine base editor system based on whole genome sequencing, and its application in gene editing, The adenine base editor system comprises a TadA:TadA*:Cas9 fusion gene and gRNA. The system can catalyze the efficient replacement of adenine (A) at a target site with guanine (G), and has a broad application prospect in human disease gene editing therapy and disease model construction. The first method for detecting the off-target effect of the ABE system in a whole genome range, called EndoV-seq method for short, is developed in the invention. The EndoV-seq method provided by the invention has a broad application prospect in the field of gene editing, especially gene editing therapy.

The invention mainly relates to a grafting method for fast cultivating camellia, which comprises the following steps of (1) cutting trunk of parental stock; (2) branch sprouting: promoting the sprouting of latent buds of the trunk cortex of the parental stock; (3) sprout curing; (4) sprout trimming: removing puny branches and evenly maintaining the strong-growing sprouts at different positions of the trunk of the parental stock; (5) split-scion graft: shearing the sprouts at the position 3-4cm away from the trunk, cutting the sprout at the center along the vertical axis, then inserting half-lignified scion into a notch, and finally fixing the branch, binding up for preserving moisture, and grafting one scion on one sprout; and (6) grafting post treatment. The grafting method has the beneficial effects: by promoting the sprouting of the branches on the trunk through cutting the trunk of the parental stock, and as the sprouts are large in quantity and evenly distributed around the trunk, the scion quantity is increased, thus being beneficial to the quick forming of the plump tree crowns; the sprouts stretch to periphery, thus maintaining the harmony of the crown shapes; and the new sprouts grow vigorously, thus being capable of improving the survival rate of graft.

The invention relates to an SNP (simple nucleotide polymorphism) modular marker for rice low cadmium accumulation gene OsHMA3; a detection site of the marker is at base 7431781 of chromosome 7 of rice. The invention also provides a primer set and method for detecting the SNP marker; the primer set has a sequence shown as in SEQ ID NO. 2-4. The SNP molecular marker and the primer set and method have the advantages that the SNP molecular marker is suitable for predicting the cadmium content of rice kernels before rice not bearing fruits, and suitable for accurate screening, such that breeding of low-cadmium-content varieties of rice is significantly promoted; the detection method is accurate and reliable and is simple to perform; the detection of SNP sites for rice OsHMA3 gene provides scientific basis for the breeding or improvement of low-cadmium-content kernel varieties of rice.

The invention relates to the technical field of molecular breeding, in particular to molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of the molecular markers. The molecular markers includes HAU1980240, NAU3177160, CGR5149280, NAU3405220 and NAU1325210. By the molecular markers, defects of current breeding techniques in fiber quality identification are overcome, fiber strength selection efficiency can be increased, and breeding process of new varieties of high-quality fibers can be accelerated.

A method and apparatus for pre-fracturing soil before the soil is cut by coulter wheels to achieve an improved cultivation apparatus wherein a penetrator bar is rotatably mounted on the front of a tillage implement and supports a plurality of adjustable S-tines for penetration of the soil prior to cultivation and resulting interaction of the fractured soil with the coulter blades.

The invention relates to a strong sand fixation and soil conservation agent for repairing degraded ecological environment as well as preparation and application methods of the strong sand fixation and soil conservation agent. The strong sand fixation and soil conservation agent is prepared from the following components including water, crop straw powder, rubber powder, plant seeds, fibers, super-absorbent resin and a compound fertilizer, wherein the weight ratio of the water to the crop straw powder to the rubber powder to the plant seed to the fiber to the super-absorbent resin to the compound fertilizer is (70-90) to (6-14) to (1.5-3.5) to (0.00014-0.0003) to (0.0001-0.0003) to (0.0003-0.0008) to (0.0003-0.0006), preferentially 85 to 12 to 2.5 to 0.00025 to 0.00025 to 0.0005 to 0.0004. According to the preparation and application methods provided by the invention, the prepared strong sand fixation and soil conservation agent is sprayed to the surface of greening ground by virtue of special equipment with a high pressure device and means including crushing, dewatering and mixing, and the plant survival rate is high. Compared with the prior art, the strong sand fixation and soil conservation agent has the advantages of simpleness in process, convenience in spraying operation, cost lower than other synthetic polymers and the like and is an ideal material for ideally controlling desertification and soil and water conservation.

The invention belongs to the field of haploid breeding technology and discloses a wheat double haploid production method by wheat-and-maize distant hybridization. The main technological characteristics of the method comprise steps of planting, induction of haploid embryo, dissection of haploid embryo, respective isolated culture of small embryo and big embryo into haploid plantlet, vernalization,chromosome doubling, transplantation and harvesting. The big embryo is cultured in big embryo culture medium. The small embryo is firstly cultured in small embryo culture medium and then cultured in basal medium. Small embryo culture medium has a higher sugar concentration, a higher osmotic pressure, richer kinds of amino acids, higher vitamin contents and contains trace of growth regulation factors with the germinating and seedling rate of small embryo being over 75%. By low-temperature vernalization, isolated cultured tissue culture seedlings are directly carried out chromosome doubling andplanting with the doubling treatment efficiency being over 89%. The method provided by the invention is adopted to improve seedling rate of haploid embryo and survival rate and success rate of chromosome doubling, simplify wheat breeding program, increase breeding efficiency and shorten breeding cycle, and has apparent effects on accelerating cultivation of fine varieties.

The present invention describes a new substrate comprising olive oil waste as the nutrient for growing edible and medicinal shiitake mushrooms (Lentinus edodes) in aseptic plastic bags. The invention further provides the strain Ile-1, a new and distinct variety of the species Lentinus edodes, that grows very efficiently in the olive oil waste-containing substrate.

The invention relates to a non-point source pollution prevention and control cultivation method for a vineyard. The method comprises the steps of carrying out plating layout on the vineyard, enabling the row spacing*line spacing of grape planting in the vineyard to be 0.8*2.5; planting European chicory and ryegrass between the lines of the grapes; carrying out real-time cutting in a growing season, applying a base fertilizer to grape trees for one time each year, and adopting the fertilization mode of drop irrigation in the remaining periods except base fertilizer application. By means of the method, the tillage frequency of the vineyard is reduced, the application quantity of a chemical fertilizer and a pesticide is reduced, the use amount of N is reduced by 32.3%, the use amount of P is reduced by 36.29%, the use quantity of the pesticide is reduced by 42.2%, suspended matter of surface runoff is reduced by 28.57%, total nitrogen and total phosphorus of the surface runoff are reduced by 25.32% and 29.51% respectively, suspended matter of underground water is reduced by 24.36%, the cost is saved by 19.38% on the basis of ensuring the stable grape yield, earnings for per hectare are increased by 16500 yuan, and agricultural non-point source pollution of the vineyard is effectively controlled.

The invention relates to a taxus mairei grafting culture method. The taxus mairei grafting culture method is characterized by comprising the following steps that (a) a 2-3-year old branch section with the light grey bark layer is cut out from a strong taxus mairei plant with needed characters, the bottom end of the branch section is cut with an art knife to form a long inclined face and a short inclined face which are symmetrical, and the bark layer of 0.4-0.5 cm is cut away from the upper end of the long inclined face to expose a cambium; (b) a common strong taxus mairei nursery stock is selected as a stock, and the smooth part on the shady face serves as a part to be grafted, wherein firstly, the knife edge cuts into the bark layer part in a 30-degree angle, the knife edge cuts downwards parallel to the stock after the white cambium is seen, and then downward cutting is continuously conducted by 2-3 cm with a 30-degree angle existing between the knife edge and the stock; (c) a scion is inserted into the notch of the stock through a left hand, the cambium at the upper end of the long inclined face of the scion is aligned with the cambium of the stock, the scion and the stock are pinched by a right hand, and a plastic thin film is used for sealing binding. The taxus mairei grafting culture method is good in grafting effect, high in survival rate and capable of rapidly culturing a large amount of nursery stocks.

The embodiments of the present invention aim to integrate two common agricultural-practices, first of hothouses cover application, and second of protective netting. A method for reversibly sealing the pores of netting by applying a sealing agent on a net in a reversible manner, thereby producing an agricultural covering to protect underlying crops, particularly from cold conditions, is disclosed. A method for reversibly opening the pores of a sealed net, by exposing said portion of the net to chemical or physical unsealing agents and thus opening at least a portion of the sealed pores of the net, is conjointly disclosed. Furthermore, a method of including additives to the sealing agent, which enhances the protective properties of the resultant covering such as its ability to repel insects, its ability to block ultraviolet or other radiation, its water proofing qualities, is also disclosed.

A method and system is provided whereby the environment of the container grown plant is controlled to replicate a natural environment. Since container grown plants are not in direct contact with the soil in the earth, a container grown plant can develop its own local electrical field and static charge that can hinder the development of the plant. A plant media grounding device is provided in which copper prongs fit into a housing that is connected to a power cord. The prongs are inserted at the plant's base in the growing media. The power cord is plugged into an outlet. The plant then becomes grounded to the earth ground of the building's power distribution system. In a further embodiment, the plant media grounding device may further include a variety of sensors in order to monitor the progress of the plant and the plant's environment.

The invention discloses a cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and a linked molecular marker. A highly cotton verticillium wilt resistance major QTL is located on a cotton D11 chromosome, and the major QTL explains 13.23-13.25% of phenotypic variation. Six SSR (Signal Sequence Receptor) markers are closely interlocked, and the SSR markers comprise SSR / NAU6593, SSR / NAU6697, SSR / NAU6675, SSR / NAU6520, SSR / NAU6530 and SSR / NAU6431. The cotton verticillium wilt resistance major QTL and the linked marker provided by the invention can be applied to screening or apprasing verticillium wilt resistant cotton varieties; furthermore, the defects that an existing breeding technology for appraising verticillium wilt resistance is high in appraisal cost, long in time, low in stability and the like, therefore new variety culture and industrial process of the new highly verticillium wilt resistance cotton varieties can be greatly accelerated.

The invention relates to the technical field of molecular breeding, in particular to molecular markers derived from sea island cotton Hai 1 and associated with the fiber length and applications of the molecular markers. The molecular markers are PGML01861(330), DPL0209(200) and CGR5565(150). According to the molecular markers and the applications, the defects of conventional breeding technology for fiber quality identification are overcome, the fiber length selection efficiency can be improved, and the cultivation process of a new high-quality variety is accelerated.

This invention is directed to a method of growing a plant from the Cannabaceae family, the cultivar or composition produced therefrom, wherein the plant is exposed to artificial lighting of different intensities based on spacing, growth phase, and flowering yield.

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

The invention discloses an SSR primer group developed based on multiple transcriptome sequences of color group zantedeschia aethiopica and acquisition method and application. The method comprises the following steps of firstly, screening polymorphic SSR primers from transcriptome splicing sequences of three varieties of the color group zantedeschia aethiopica by utilizing a CandiSSR method, determining hundreds of polymorphic SSR primers after experimental verification, and then carrying out polymorphic analysis on more than 160 parts of color group zantedeschia aethiopica varieties by utilizing 35 pairs of screened SSR primers with high polymorphism. In addition, on the basis of the research, 160 core germplasm of the color group zantedeschia aethiopica is constructed through a maximization strategy, finally, 30 optimal representative parents of the color group zantedeschia aethiopica are screened out, and good help is provided for screening of core parents of the color group zantedeschia aethiopica, improvement of breeding efficiency and accelerated cultivation of new varieties with proprietary intellectual property rights in the future.

The invention relates to the technical field of plant growth regulators, and particularly discloses a nursery stock growth promoting and thickening agent. The nursery stock growth promoting and thickening agent is formed by mixing the following raw materials, by weight, 20 percent to 40 percent of chlormequat chloride, 0.1 percent to 0.2 percent of alpha-pimacol, 0.05 percent to 0.1 percent of indolebutyric acid potassium, five percent to 10 percent of monopotassium phosphate and the balance fillers. The nursery stock growth promoting and thickening agent can promote the growth of nursery stocks well, so that the nursery stocks are thick and strong in stem, developed in root system, high in lignification degree and reasonable in seedling height, and the wind resisting capacity and other adversity tolerating capacity of the nursery stocks are improved.

The invention relates to an organic garbage reduction treatment device, belonging to the technical field of environmental protection, and aiming at solving the problems that in the prior art, an organic garbage treatment technology is complicated and high in cost. The organic garbage reduction treatment device comprises a feeding system, a pretreatment system and a reduction treatment system which are sequentially connected, wherein the reduction treatment system comprises a stock bin, a heating device arranged at the outer side of the stock bin, a stirring device, a waste gas treatment device arranged on the top of the stock bin, and a waste water treatment device and a discharge hole which are respectively arranged at and formed in the bottom of the stock bin; the reduction treatment system also comprises an automatic dosing unit and an oxygen replenishment device which are arranged at the outer side of the stock bin. According to the organic garbage reduction treatment device, solid organic garbage is treated by crushing, carrying out solid-liquid separation and sterilizing at high temperature; after that, strains are added into the treated organic garbage, and the organic garbage is filled with oxygen so as to be fermented, so that the organic garbage is converted into an organic fertilizer; therefore, the reduction, recycling and harmless treatment of the organic garbage are realized.

The invention discloses a rice grain length and grain weight related protein as well as a coding gene and application thereof. According to the invention, an OsMOB1A gene is introduced into wild typerice by using a recombinant vector to obtain transgenic rice. Compared with the wild type rice, for the transgenic rice, the OsMOB1A gene is overexpressed, the grain length of the rice is obviously increased, and the grain weight is obviously increased. Therefore, the OsMOB1A gene is related to the grain shape, and a foundation is laid for improving the cultivation of transgenic plants with changed grain shapes. The invention has important theoretical significance and practical significance for further clarification of a molecular mechanism of plant grain development and cultivation of high-quality and high-yield new crop varieties through genetic engineering means.

The invention discloses a method for quickly a cultivating steamed bread willow by taking a bamboo willow as tree stock. By the method, a problem that a traditional steamed bread willow propagation method has the high cultivating cost is solved. The method comprises the following steps of: A, selection of tree stock, selecting healthily-growing bamboo willow cutting seedlings or inserted-bar afforestation seedlings with heights above 3m and breast diameters above 2cm and without plant diseases or insect pests as the tree stock for grafting the steamed bread willows; B, grafting and collecting, grafting the steamed bread willow along collection, and cutting off annual branches of the steamed bread willow with plump buds, strong growth and roughness of 0.6cm-1cm; C, grafting the steamed bread willow by a crown grafting or cut-grafting method; D, managing after grafting. In the method, the bamboo willow is taken as the tree stock and is used for grafting and cultivating the steamed bread willow, so that a cultivating period can be greatly shortened, a cultivation cost is reduced, large seedlings are quickly cultivated and the economic benefit of a nursery garden is increased.

The invention discloses a high-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties. The method disclosed by the invention comprises the steps: by utilizing parting results of high-density molecular markers, in combination with related markers of important characters such as stress resistance, quality, yield and the like of the core genealogy varieties andgenome zone information, a hybrid breeding strategy which takes a construction core genealogy variety as a main body and introducing excellent genes and characters of other varieties is established;existing high-quality variety resources are integrated to achieve quick and directional breeding. The method disclosed by the invention greatly reduces seed selection blindness and randomness, improves breeding efficiency and reduces breeding cost. Whole-genome high-density SNP marking is utilized to perform assistant selection, so that the choosing efficiency is improved; meanwhile, obtained derived variety phenotype information and genetic information bring convenience to following pyramiding breeding. In a pyramiding breeding process, more high-quality genetic resources are introduced, an optimal fragment combination pattern is continuously optimized and improved, and breeding of varieties in which more protogene and fragments are pyramided is accelerated.

The invention relates to the technical field of molecular genetics and crop molecular breeding, in particular to a molecular marker linked with a major quantitative trait locus (QTL) of the hundred-kernel weight of arachis hypogaea and application of molecular marker. The molecular marker includes a molecular marker Ah011476, and / or, a molecular marker Ah011478; the molecular marker Ah011476 is the 14206230 site on chromosome B06 of arachis hypogaea, and polymorphism is G or T; and the molecular marker Ah011478 is the 17650027 site on chromosome B06 on the arachis hypogaea, and polymorphism is C or A. According to the invention, the molecular markers linked with the arachis hypogaea hundred-kernel weight major QTL are obtained by screening, and the molecular markers can be used for effectively identifying the hundred-kernel weight of the arachis hypogaea, and are used for genetic improvement of the arachis hypogaea to obtain an arachis hypogaea variety with high hundred-kernel weight.

The invention provides a genome selection method of residual feed intake of white feather broilers. GEBV estimation is carried out on the residual feed intake (RFI) of an important feed conversion character index by utilizing a one-step whole-genome breeding value (GEBV) estimation method integrating a remarkable SNP marker effect. The invention further provides an SNP marker remarkably related tothe residual feed intake character of the white feather broiler chicken and application of the SNP marker. The SNP marker comprises the marker rs13649171, the marker rs740268684, the marker rs312607889, the marker rs314437326 and the marker rs313748618. Compared with a conventional one-step GEBV estimation result, the selection accuracy of the breeding method of the white feather broiler chickenline with the low residual feed intake character can be improved by 15.44%. The method can shorten the new strain breeding time, save the breeding cost, improve the breeding efficiency and acceleratethe white feather broiler chicken autonomous strain breeding process in China.

The invention discloses an automatic nutrient solution replenishing device for a multi-layer seedling raising rack. The automatic nutrient solution replenishing device comprises a support, a solution receiving box and a solution storage box, wherein the top of the support is fixedly connected with the bottom of the solution receiving box. The invention relates to the technical field of seedling raising racks. According to the automatic nutrient solution replenishing device for the multi-layer seedling raising rack, when a nutrient solution in a nutrient solution tray is absorbed and reduced, through the cooperation of a movable plate and a counterweight, the counterweight is pressed down to pull a pulling string, a piston block is separated from the bottom of the solution storage box, the nutrient solution enters the nutrient solution tray, it is not required that workers add the nutrient solution every day, great manpower is liberated, and time is saved. When the nutrient solution in the nutrient solution tray is reduced, a floating plate can be lowered along with the reduction of the nutrient solution, a clamping block is separated from a solution conveying pipe, so that the nutrient solution in the solution conveying pipe enters the nutrient solution tray, and seedling culture is improved; it is only required that the solution storage box is filled with the nutrient solution every day, the workers do not worry about the situation that seedling loss is caused by negligence, and the working efficiency is greatly improved.

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

The invention discloses a photorespiration metabolism modified branch and an application of the photorespiration metabolism modified branch in C3 plant, which relate to the technical field of a plantgenetic engineering. The branch comprises three proteins of OsGLO3, OsCAT2 and OsOXO3. The branch can modify the C3 plant photorespiration, so that photorespiration is reduced, the photosynthetic efficiency is increased, and the plant biomass is increased. The experiment result shows that GOC photorespiration metabolism modified branch transferred to the plant appears that photosynthetic rate is increased, the biomass is increased, and chlorophyll is increased. The photorespiration metabolism modified branch has deep meaning for explicating high-light effect mechanism, producing crops and renewable energy sources, and reducing CO2 discharge, and during the practical application, the GOC photorespiration metabolism modified branch is transferred to different C3 plants, so that high-yield variety can be cultured.