248results about How to "Easy Survival" patented technology

The invention discloses a family of peptides termed NRP compounds or NRPs that can promote neuronal migration, neurite outgrowth, neuronal proliferation, neural differentiation and / or neuronal survival, and provides compositions and methods for the use of NRPs in the treatment of brain injury and neurodegenerative disease. NRP compounds can induce neurons and neuroblasts to proliferate and migrate into areas of damage caused by acute brain injury or chronic neurodegenerative disease, such as exposure to toxins, stroke, trauma, nervous system infections, demyelinating diseases, dementias, and metabolic disorders. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly, and directly into the nervous system of a patient. NRP compounds can be formulated into pharmaceutically acceptable dose forms for therapeutic use. Methods for detecting neural regeneration, neural proliferation, neural differentiation, neurite outgrowth and neural survival can be used to develop other neurally active agents.

In one aspect, the invention provides vision prosthesis systems. Exemplary vision prosthesis systems of the invention comprise a light energy generator operably connected to a wearable head piece comprising a device for directing light energy produced by the light energy generator onto a mammalian retina, wherein the light energy generator is tuned to emit light energy of sufficient power to modulate neural activity in the retina. In another aspect, the invention provides methods for irradiating neurons in the retina of the mammalian eye by directing light energy produced by a light energy generator onto a mammalian retina. The methods of the invention may be used to directly modulate the activity of retinal neurons or to introduce molecules into retinal cells.

Disclosed are methods for conditionally immortalizing stem cells, including adult and embryonic stem cells, the cells produced by such methods, therapeutic and laboratory or research methods of using such cells, and methods to identify compounds related to cell differentiation and development or to treat diseases, using such cells. A mouse model of acute myeloid leukemia (AML) and cells and methods related to such mouse model are also described.

The present invention relates to female germline stem cells and their progenitors, methods of isolation thereof, and methods of use thereof.

The subject invention relates to viruses that are able to purge (reduce or eliminate) undesirable cells in a mixture of cells. Undesirable cells can include neoplastic cells, cells mediating graft-versus host diseases, and autoimmune cells. The subject invention also relates to the purging of undesirable cells from bone marrow or peripheral blood cell harvests in the treatment of mammals including cancer patients, transplant recipients, and patients with autoimmune disease.

Disclosed are methods for conditionally immortalizing stem cells, including adult and embryonic stem cells, the cells produced by such methods, therapeutic and laboratory or research methods of using such cells, and methods to identify compounds related to cell differentiation and development or to treat diseases, using such cells. A mouse model of acute myeloid leukemia (AML) and cells and methods related to such mouse model are also described.

The invention is directed to T cells and other cells that express chimeric NK-p30 receptors (“chimeric NKp30 T cells”), methods of making and using chimeric NKp30 T cells, and methods of using these chimeric NKp30 T cells, isolated populations thereof, and compositions comprising the same. In another aspect, said chimeric NKp30 T cells are further designed to express a functional non-TCR receptor. The disclosure also pertains to methods of making said chimeric NKp30 T cells, and methods of reducing or ameliorating, or preventing or treating, diseases and disorders using said chimeric NKp30 T cells, populations thereof, or compositions comprising the same.

The invention discloses a family of peptides termed NRP compounds or NRPs that can promote neuronal migration, neurite outgrowth, neuronal proliferation, neural differentiation and / or neuronal survival, and provides compositions and methods for the use of NRPs in the treatment of brain injury and neurodegenerative disease. NRP compounds can induce neurons and neuroblasts to proliferate and migrate into areas of damage caused by acute brain injury or chronic neurodegenerative disease, such as exposure to toxins, stroke, trauma, nervous system infections, demyelinating diseases, dementias, and metabolic disorders. NRP compounds may be administered directly to a subject or to a subject's cells by a variety of means including orally, intraperitoneally, intravascularly, and directly into the nervous system of a patient. NRP compounds can be formulated into pharmaceutically acceptable dose forms for therapeutic use. Methods for detecting neural regeneration, neural proliferation, neural differentiation, neurite outgrowth and neural survival can be used to develop other neurally active agents.

Provided herein are chimeric antigen receptors (CARs) for cancer therapy, and more particularly, CARs containing a scFv from a CD33 monoclonal antibody. Provided are immune effector cells containing such CARs, and methods of treating proliferative disorders such as acute myeloid leukemia (AML), and relapsed or refractory AML.

This invention generally relates to methods for the production of antibody producing cells and antibodies in protooncogene expressing animals. The invention also relates to methods for the efficient production of antibodies specific for antigens that are normally subject to immunological constraints such as self tolerance. The invention further relates to the production of antibody producing cells and antibodies without the need for the conventional fusing of antibody producing B cells with a myeloma fusion partner.

The invention relates to a plant remediation technique for heavy metal contaminated soil and discloses the use of urticaceae plants in remediation of cadmium contaminated soil, in particular the use of Debregeasia orientalis C. J. Chen in remediation of cadmium heavy metal contaminated soil, which is to plant Debregeasia orientalis C. J. Chen in the heavy metal contaminated soil. Based on the cadmium enriching characteristic of Debregeasia orientalis C. J. Chen, the Debregeasia orientalis C. J. Chen is planted in the cadmium contaminated soil to absorb accumulated cadmium heavy metal and transfer most cadmium heavy metal to the parts above group, the parts above the group are harvested and ashed to extract heavy metal, and thus, the cadmium contaminated soil is remediated. In the growing process of the Debregeasia orientalis C. J. Chen, plant management is not required and stools can be left for growing braches, so the cost is low and the operability is high; meanwhile, new plant resource varieties are development for plant remediation of heavy metal contaminated soil, and new improvement and development space is provided for the deep development of the plant remediation technique.

The invention discloses a fast efficient propagation method of Paris polyphylla. The fast efficient propagation method of Paris polyphylla includes: selecting and treating propagating materials; inducing latent buds to germinate by stratification; and managing seedling propagation and production. The fast efficient propagation method of Paris polyphylla has the advantages that seedling growth is fast, propagation coefficient is high, and seedling quality is high. The latent buds are induced to germinate by inhibiting apical dominance without damaging completeness of rhizomes, stem cutting for seedling separation is performed according to germinating latent bud spots, and accordingly many latent buds germinate, and many separate seedlings are grown. Seedling rate is one to two times higher than that in the traditional way that segmental cutting is performed prior to inducing the latent buds to germinate. When the latent buds are induced to germinate, the rhizomes are not damaged. Therefore, seedlings from the latent buds are robust. By sterilization and seed soaking prior to seedling raising after cutting and seedling separation, strong seedlings are easy to obtain and survive easily after planted in fields.

The invention belongs to the technical field of vegetable seedling culture and recycling of solid organic waste, and particularly relates to a vegetable seedling culture soilless matrix compounded with straw mushroom slag and a preparation method thereof. The vegetable seedling culture soilless matrix compounded with the straw mushroom slag comprises the main ingredients: straw mushroom slag, vermiculite and coconut residuum and the like. In the matrix, the volume ratio of the decomposed straw mushroom slag accounts for more than 40% of the total volume, the volume ratio of the vermiculite accounts for less than 305 of the total volume, the volume ratio of the coconut residuum accounts for less than 30% of the total volume, the matrix is prepared by mixing all the ingredients, and the volume weight is about 0.5g / cm<3>. In the invention, due to the application of technologies such as higher adsorption properties and nutrient compounding and the like, the technical bottleneck of restricting the quality of the vegetable seedling culture soilless matrix is effectively solved, the germination rate of seeds is increased, the forming of strong seedling is enhanced, and the seedling culture period is shortened.

The invention discloses a method for repairing heavy metal pollution in mine soil and sludge. The method is characterized in that wild garden chrysanthemum is planted in the mine soil and sludge polluted by the heavy metal, the heavy metals accumulated in soil such as Cd, Cu, Pb, Zn, and the like, are absorbed by adopting the cumulation property of the wild garden chrysanthemum to four heavy metals such as Cd, Cu, Pb and Zn, then most of the four metals are transferred to the overground part, thus achieving the purpose of repairing heavy metal pollution in mine soil.

The present invention relates generally to methods for promoting regeneration, outgrowth and survival of dopaminergic neurons comprising contacting said dopaminergic neurons with an effective amount of a composition comprising an Sp35 antagonist. Additionally, the invention is related generally to methods of treating various diseases, disorders or injuries associated with dopaminergic neuronal degeneration or death by administration of an Sp35 antagonist.

Compounds comprising R-G-Cysteic Acid (i.e., R-G-NH—CH(CH2—SO3H)COOH or Arg-Gly-NH—CH(CH2—SO3H)COOH) and derivatives thereof, including pharmaceutically acceptable salts, hydrates, stereoisomers, multimers, cyclic forms, linear forms, drug-conjugates, pro-drugs and their derivatives. Also disclosed are methods for making and using such compounds including methods for inhibiting integrins including but not necessarily limited to α5β1-Integrin, αvβ3-Integrin and αvβ5-Integrin, inhibiting cellular adhesion to RGD binding sites, preventing or treating viral or other microbial infections, inhibiting angiogenesis in tumors, retinal tissue or other tissues or delivering other diagnostic or therapeutic agents to RGD binding sites in human or animal subjects.

Transposase polypeptides and polynucleotides are provided, which have a high activity in mammalian cells. Methods for engineering cells, such as chimeric antigen T-cells, with the transposes are also provided.

The invention discloses a flue-cured tobacco mulch pyramidic hole transplanting method and a structure after flue-cured tobacco mulch pyramidic hole field-planting. The flue-cured tobacco mulch pyramidic hole transplanting method comprises the following steps of: a, digging a pyramidic tobacco seedling planting hole on a ridge for planting tobacco, wherein the size and shape of a pyramidic tip part of the pyramidic tobacco seedling planting hole are same as those of a matrix hole of a floating seedling-raising disc; b, placing the tobacco seedling cultured by the floating seedling-raising disc into the pyramidic tobacco seedling planting hole; and c, covering a mulch. According to the invention, the ridge is provided with the pyramidic planting hole, so that the lower end of the planting hole with the shape can be matched with the shape of a planting cup of the floating seedling-raising disc, thus the tobacco seedling can be directly placed in the planting hole during planting, so that procedures, such as seedling supporting and ridging are omitted during conventional planting, and human hands are prevented from being burnt by tobacco seedlings; and by adopting deephole planting which not only has a good effect and is superior to big hole digging for planting and small arch building for planting, but also can avoid the procedures of digging a big hole and building and dismantling a small arch, namely, the deephole planting can be finished by the conventional mulch and the conventional pavement method.

A graft technique for reproducing lectuce tea tree includes cutting off a branch of pure lectuce tea tree as scion with 4-6 cm in length, sharpening its one end from both sides, choosing a pure lectuce tea tree with (1-2)-year old as stock, cutting to form an incision, grafting, binding and cultivation. Its advantages are high adaptability and survival rate, high growth speed, and high tea quality.

Compounds comprising R-G-Cysteic Acid (i.e., R-G-NH—CH(CH2—SO3H)COOH or Arg-Gly-NH—CH(CH2—SO3H)COOH) and derivatives thereof, including pharmaceutically acceptable salts, hydrates, stereoisomers, multimers, cyclic forms, linear forms, drug-conjugates, pro-drugs and their derivatives. Also disclosed are methods for making and using such compounds including methods for inhibiting cellular adhesion to RGD binding sites or delivering other diagnostic or therapeutic agents to RGD binding sites in human or animal subjects.

The invention discloses application of grain amaranth in repairing mine soil and sludge polluted by heavy metal cadmium, wherein the grain amaranth only needs to be planted in the mine soil or the sludge polluted by heavy metal cadmium. By using the characteristic that the grain amaranth enriches the heavy metal cadmium, the grain amaranth is planted in the mine soil or the sludge to be capable ofabsorbing the accumulated heavy metal cadmium and transmitting the most of the heavy metal cadmium to overground part to achieve the aim of repairing the mine soil and the sludge which are polluted by the heavy metal cadmium. The grain amaranth does not need special management in the process of growing, therefore, the invention has the characteristics of less investment, low cost, strong operability, less disturbance to soil environment, permanent managing effect, not breaking site structure, not causing secondary pollution, wide application area, simple post processing, and the like; and thegrain amaranth has large individual biomass, can improve the effect for repairing the heavy metal in unit area, and provides a new plant resource variety for repairing the soil polluted by the heavymetal cadmium.

Provided herein are compositions and methods for isolating and culturing cardiac progenitor cells, and for improved transplantation.

The invention discloses a method for constructing a polyethylene grass planting terrace. The method comprises the following specific steps: construction preparation, mensuration positioning, the removal of surface oil, the leveling and tamping of plain soil, the paving of a gravel layer, the paving and leveling of a mixed layer; the paving of a grass planting plate or a grass planting grid, twice filling of planting soil, the paving of greensward or the sowing of grass seeds and watering and maintenance. The polyethylene grass planting terrace constructed through the method has the characteristics that the terrace substantially improves the virescence rate, has high bearing capacity, solves the problem of difficult parking, has long service life, energy conservation, environmental protection, light weight and convenient and rapid conveying and mounting, repeatedly recycles and reduces mounting cost.

The invention relates to the field of ecological restoration and reconstruction of seagrass meadows, in particular to a method and device for transplanting zostera marina L.. The device is provided with brackets and zostera marina L. fixing grids, wherein the zostera marina L. fixing grids are put in the brackets; and one or a plurality of zostera marina L. fixing grids are put in each bracket. The method comprises the following steps: (1) fixing the zostera marina L. on the zostera marina L. fixing grids; (2) after the zostera marina L. plants are fixed on the zostera marina L. fixing grids, vertically clamping grooves at the two ends of a main plate on two spaced cross beams of the brackets; and (3) utilizing the device to transplant seagrass in the sea area. The method and the device have the following beneficial effects: the problem that the seagrass in the sea area in the subtidal zone is difficult to transplant is solved, and in combination with the simple, convenient and effective device, restoration and reconstruction of the zostera marina L. seagrass meadows in the subtidal zone can be conveniently and rapidly carried out; and meanwhile, the device can be recycled, thus saving the cost, and the used strings are made of degradable plant materials, thus not polluting the sea area.

The invention discloses an appreciating landscape pavilion type tree crown modeling method. The method aims to solve the problems that a part of landscape trees are singular in crown type, lack of attractiveness and poor in ornamental value. According to the grafting modeling method, inverse budding is carried out on a center tree, budding is carried out on stand column trees, inverse budding and budding branches are subjected to inarching, and a pavilion type tree crown is formed. A unique model is formed, and artistic and ornamental value of landscape woods can be obviously improved. Natural corners are formed through the inverse budding method, perfect inarching of the corners and grafted sprouts on stand columns is achieved, and the corners and the grafted sprouts grow into a whole. According to the inarching method, contact faces formed by notches are large, formed union faces are large, and trees can easily survive. In addition, the branches after inarching are not completely cut off from parent bodies, the original shapes of parent body roots are maintained, only a part of grafted stems are cut off, then the cut parts of the branches and the cut parts of the grafted stems are tightly combined, and due to the fact that root systems of two trees absorb nutrition, the inarching survival rate is high.

The invention discloses application of feverfew siegesbeckia herb in remediation of soil contaminated with the heavy metal cadmium, which is realized through planting siegesbeckia herb in soil contaminated with the heavy metal cadmium. The siegesbeckia herb has an excellent property of accumulating the heavy metal cadmium, can absorb and accumulate the heavy metal cadmium, and transfer most of the heavy metal cadmium above the ground in order to realize remediation of soil contaminated with the heavy metal cadmium through being planted in the soil. The invention not only has the characteristics of low cost and strong operability because the siegesbeckia herb needs no special treatment during the growing process but also can increase the remediation effect of heavy metal in soil of unit area because the siegesbeckia herb has a large quantity, and develops a new plant resource applied for plant remediation of soil contaminated with heavy metal.

The invention discloses application of pilea cadierei in treating livestock and poultry breeding wastewater, belonging to wastewater treatment filed. In the invention, the characteristics that of pilea cadierei has cold resistance, does not fall leaves or wither in winter and can absorb and remove ammonia nitrogen, total nitrogen and other pollutants in livestock and poultry breeding wastewater is utilized, and the pilea cadierei is directly transplanted or inserted by cuttage in the environment of livestock and poultry breeding wastewater to absorb the pollutants of accumulated wastewater so as to purify the livestock and poultry breeding wastewater. The invention solves the problem that most of plants for purifying wastewater have poor purification effects in winter currently and also provides new types of plant resources for the treatment of livestock and poultry breeding wastewater pollution, brings new improvement and development space for the treatment of livestock and poultry breeding wastewater pollution by artificial wetland plants, and achieves obvious advantages in operation cost, operation maintenance, treatment effect, as well as brought economical benefits in landscape, environmental protection and medical application, compared with traditional plants for purifying wastewater.

The invention discloses application of malva to remediation of cadmium and lead pollution of mine soil and sludge, which is characterized by planting malva in the mine soil or sludge polluted by heavy metals. The invention has the following beneficial effects: the malva is planted in the mine soil or sludge by utilizing the enrichment characteristics of malva toward cadmium, lead and other heavy metals, and can absorb the accumulated heavy metals such as cadmium, lead, etc, and most malva is transported above the ground to achieve the aim of remediating heavy metal pollution of mine soil and sludge; the malva dispenses with special management in the growth process, thus the cost is lower and the operability is strong; and meanwhile, the invention develops new plant resource varieties for remediation of plants polluted by heavy metals in the mine oil and sludge and brings new progress and development space for deep development of plant remediation technology.

The invention discloses a method for restoring copper-polluted soil by plants. The method provided by the invention is characterized in that dayflower seedlings subjected to cutting propagation are planted in copper-containing soil, grow and are harvested or are pulled up during harvest time; and copper is recovered. Concretely, the method comprises the following steps of 1, cutting off stems of dayflower plants, carrying out cutting propagation of the stems by a cutting bed according to plant and row distances of 5cm*10cm, and keeping soil moist after cutting, 2, flattening a sample area in copper-polluted zone, and digging planting holes, 3, planting dayflower seedlings rooting in the cutting bed in copper-containing soil, 4, harvesting overground parts of the dayflower seedlings or pulling up the whole dayflower seedlings with roots, and 5, drying and crushing the harvested overground parts of the dayflower seedlings or the whole dayflower seedlings with roots, and carrying out copper recovery. The method provided by the invention has the advantages of low cost, high restoration efficiency and low environmental risk, and can be utilized for restoration of copper-polluted soil in industry and agriculture and for reconstruction of ecological vegetation in a mine tailing area.

The invention relates to a rapid propagating method of Chinese tamarisk seedlings in summer, which comprises the following steps: (1) selecting Chinese tamarisk branches which are not completely lignified in the year, and scissoring into cuttage branches; (2) cutting the cuttage branches into a nutrition pot holding sand soil, and placing in a greenhouse nursery, wherein the temperature inside the greenhouse nursery is 20-30 DEG C; (3) carrying out water spraying treatment on the cuttage branches to keep the leaf surface wet; and (4) domesticating the seedlings 10-20 d before the seedlings leave the nursery to obtain the Chinese tamarisk seedlings. By adopting the method provided by the invention, rapid propagation of Chinese tamarisk seedlings from June to September can be realized, and the Chinese tamarisk branches which are not completely lignified are used as cuttage branches, so that the survival rate is high and the propagation rate is high.