The invention discloses a 
tissue culture and rapid propagation method of 
Hibiscus mutabilis Linn. The 
tissue culture and rapid propagation method comprises: (1) 
bud induction culture: 
cutting Hibiscusmutabilis Linn stem section with 
bud point as explant, sterilizing, inoculating into an induction culture medium, and culturing until axillary buds germinate and grow to achieve a certain length; (2)rooting culture: 
cutting the axillary buds obtained in the step (1), inoculating into a rooting culture medium, and culturing to form rooted 
seedling; (3) proliferation culture: 
cutting the single-
bud stem section or stem apex of the rooted 
seedling obtained in the step (2), inoculating into a proliferation culture medium, and culturing to form a 
Hibiscus mutabilis Linn 
seedling; and (4) seedlingexercising and 
transplantation: transferring the 
Hibiscus mutabilis Linn seedling obtained in the step (3) to the outside, carrying out closed flask seedling exercising for 2-3 d, 
transplanting to aseedbed, and carrying out film covering and sunshade net covering culture for 8-12 d until the new root of the 
Hibiscus mutabilis Linn seedling grows, and removing the film covering and sunshade net covering culture. According to the present invention, with the 
tissue culture and rapid propagation method, the growth can be promoted, the 
survival rate of the cultured seedlings is high, the rootingrate is up to 100%, the culture time is short, and the rapid propagation of 
Hibiscus mutabilis Linn can be achieved.