The invention discloses a preparation method of a 
fondaparinux sodium injection composition. The preparation method comprises the following steps of 1) according to a prescription of the fondaparinuxsodium injection composition, weighing all materials by prescription dosage; 2) taking 
fondaparinux sodium and 
sodium chloride, combining, then adding injection water with the weight being 75-85% of the total weight, after stirring and dissolving, adopting a pH 
regulator to regulate the pH to 5-8, then adding remaining injection water, stirring and mixing uniformly to obtain liquid 
medicine; 3) filling prepared liquid 
medicine, and carrying out high-temperature sterilization, cooling, lamp inspection, 
package and inspection to obtain the 
fondaparinux sodium injection composition. The fondaparinux 
sodium injection composition prepared by the invention has the advantages that by optimizing the pH and 
sterilization process of the fondaparinux 
sodium injection composition, the obtained solution is good in 
clarity and conforms to all detection requirements of the injection liquid item in 
Chinese pharmacopoeia, so that the quality stability of products is greatly improved, the 
crystallization and the 
turbidity of the solution are reduced, and the generation of impurities is reduced.