The invention discloses a preparation method of a one-way wet conduction nano-
fiber multilayer 
composite membrane with the wettability gradient. The method comprises the following steps that hydrophilic nano materials are dispersed in 
solvent, the nano materials are dispersed uniformly through 
ultrasound, a hydrophilic 
polymer is dissolved in the dispersing agent, a 
spinning solution A1 is obtained, and a hydrophilic nano-
fiber membrane is deposited on a receiving base material through an electrostatic 
spinning method; hydrophilic nano materials are dispersed in 
solvent, the nano materials are dispersed uniformly through the 
ultrasound, a hydrophilic 
polymer is dissolved in the dispersing agent, and a 
spinning solution A2 is obtained; a 
hydrophobic polymer is dissolved in 
solvent, and a spinning solution B1 is obtained; the two spinning solutions are deposited on the hydrophilic nano-
fiber membrane to form at least one diversion layer through the electrostatic spinning method; a 
hydrophobic polymer is dissolved in solvent, a spinning solution B2 is obtained, a hydrophobic nano-fiber membrane is deposited on the diversion 
layers through the electrostatic spinning method, and the one-way wet conduction nano-fiber multilayer 
composite membrane with the wettability gradient is obtained.