The invention discloses a 
tissue culture rapid propagation method for illigera trifoliata. The 
tissue culture rapid propagation method for illigera trifoliate comprises the following steps: (1) taking a stem with a 
bud of the illigera trifoliate as an explant, inoculating the stem with the 
bud into a propagation and rejuvenation integrated culture medium, culturing in a culture room for 15 days under the indoor conditions of 22-26 DEG C, 1,400 
lux and 8 h / d, and then continuing culture under the 
natural illumination conditions of 25-28 DEG C and 3,500-4,500 
lux for 15 days to obtain cluster buds; (2) inoculating the cluster buds cultured in the step (1) in a rooting and 
seedling hardening-off synchronization culture medium, culturing the cluster buds for 7 days under the indoor conditions of 22-26 DEG C, 1,500 
lux and 10 h / d, and then culturing for 25 days in a 
greenhouse under the natural light, wherein the temperature is 23-28 DEG C, the light shading degree is 20-40%, and the 
relative humidity is 60-80%; and (3) 
transplanting. By the method, the production period of seedlings can be shortened remarkably, the production cost of the seedlings is reduced, the 
survival rate of the seedlings is increased and the quality of the seedlings is improved.