85results about How to "Improve specific recognition ability" patented technology

The invention discloses molecularly imprinted polymers (MIPs) for detecting melamine and a preparation method thereof. The MIPs are prepared by the steps as follows: cyromazine is used as a virtual moulding board and is polymerized with a functional monomer, a cross linker agent, a pore-foaming agent and an initiator at the temperature from 50 to 80 DEG C to obtain a bulk polymer; and after being processed by the operations of pulverization, screen separation, moulding board removal by acetic acid methanol and methanol, the bulk polymer is processed by vacuum drying to prepare the MIPs which have high cross reaction and outstanding selectivity to melamine. The invention selects the cyromazine as the virtual moulding board to prepare the MIPs, and avoids the problem that the melamine is hard to dissolve in a polymerization system and the problem that when the MIPs prepared by using the melamine as the moulding board is used for solid phase extraction of fill materials, moulding board leakage can lead to an inaccurate result; the invention selects the mixed system of methanol and water as the pore-foaming agent, and the prepared MIPs have high compatibility with the melamine in an aqueous solution; and used as a sample pre-processing material for analyzing the melamine, the prepared MIPs have broad application prospect.

The invention discloses a double recognition dopamine imprinting electrochemical sensor and a preparation method and application thereof. The method comprises the following steps: (1) conducting contact reaction on 3-amino boronic acid, 2,5-dimethoxy furan and acetic acid to prepare functional monomers; (2) in the presence of a buffer solution, mixing the functional monomers and dopamine to form a polymerization liquid, placing a glassy carbon electrode in the polymerization liquid, and conducting scanning preparation of a polymer film modified electrode in the polymerization liquid in accordance with the a cyclic voltammetry; and (3) placing the polymer film modified electrode in an acidic solution for electrochemical elution, so as to prepare the double recognition dopamine imprinting electrochemical sensor. The electrochemical sensor provided by the invention has excellent specificity recognition ability and anti-interference ability for the detection of dopamine.

The invention relates to a specific detection method of human breast cancer cells MCF-7 based on surface-enhanced Raman spectroscopy. MCF-7 cells act with a probe compound Rh6G-Au-Ag-aptamer, a characteristic absorption peak of Rh6G appears on the obtained Raman spectrum, and MCF-7 cells are detected by surface-enhanced Raman spectroscopy. The probe compound is prepared by the steps of using nucleic acid apatamer S2.2 as a template, depositing gold and silver bimetal alloy nanoparticles on the bases of the DNA chain of the nucleic acid apatamer S2.2 by using a photocatalysis method, and carrying a Raman signaling molecule Rh6G on the surface of the nucleic acid apatamer S2.2. The method is based on the probe compound which is in target binding with the MCF-7 cells and has high surface-enhanced Raman activity, and carries out specific detection of MCF-7 cells by using the specific target binding capacity of the apatamer with the MCF-7 cells and the enhancement effect of the alloy nanoparticles on the surface of the apatamer on the Raman signal of the Rh6G molecule.

The invention discloses a cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and a method for preparing the cell probe complex. The cell probe complex is characterized in that nucleic acid aptamer capable of realizing a targeting effect with the MCF-7 cells is used as a template, bimetal alloy nano particles of gold and silver are deposited on a basic group of a DNA (deoxyribose nucleic acid) chain of the nucleic acid aptamer by a photocatalysis method, and Raman signal molecules rhodamine 6G (Rh6G) is fixedly loaded on the surface of each nano particle. The structure of the probe complex is chain-shaped as shown by a photo of a transmission electron microscope, and the probe complex is capable of realizing the targeting effect with the MCF-7 cells. Characteristic absorption peaks of the rhodamine 6G appear on the Raman spectrum under the effect of the MCF-7 cells and the probe complex, and the human breast cancer MCF-7 cells can be effectively, sensitively and specifically detected.

The invention discloses a combined detection kit for HCV antigen-antibody. The kit comprises a first monoclonal antibody against HCV core, a first HCV recombinant chimeric antigen, a first diluted sample solution, a second diluted sample solution, a first enzyme-labeled second monoclonal antibody against HCV core antigen, a first ligand-labeled second HCV recombinant chimeric antigen, a second enzyme-labeled second ligand, a developer and a stopping solution, wherein the first diluted sample solution comprises a reducing agent, a first surfactant and a first denaturant; and the second diluted sample solution comprises a second surfactant and a second denaturant. The combined detection kit for the HCV antigen-antibody opens mismatched disulfide bonds in coating antigen and in an antigen-antibody complex of a sample in virtue of the first diluted sample solution, and destroys HCV core antigen and HCV antibody compounds in virtue of the second diluted sample solution, so more antigen is released; and thus, detection sensitivity is improved.

The invention discloses a rapid detecting sensor for microcystins. The rapid detecting sensor utilizes a photochemistry polymerization method or an electrochemical polymerization method to synthesize a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is reduced, and the specific adsorption capacity is strengthened; meanwhile, a Lamb wave piezoelectric membrane is utilized as an effector of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, the cost is reduced, the performance of the sensor is strengthened, the trace detection of the microcystins is realized, and thus the rapid detecting method for the microcystins, with high sensitivity, rapidness, low cost and low operation requirements, is established.

The invention discloses a layered fluorine-containing metal-organic framework material for adsorbing and separating acetylene ethylene, the structural general formula is ML2A, M is a metal ion, L is abroken line type organic ligand, A is an inorganic fluorine-containing anion, and the metal ion M is at least one of Cu<2+>, Fe<2+>, Co<2+>, Ni<2+> and Zn<2+>, wherein the broken line type organic ligand L is at least one of 4, 4'-dipyridylsulfoxide and 4, 4'-dipyridylsulfone, and the inorganic fluorine-containing anion A is at least one of SiF6<2->, GeF6<2->, ZrF6<2->, SnF6<2->, TiF6<2->, NbOF52<->, WO2F4<2-> and MoO2F4<2->. The layered fluorine-containing metal-organic framework material can be prepared by adopting an interface diffusion method, a solvothermal method or a room-temperature stirring method. The layered fluorine-containing metal-organic framework material is used as an adsorbent and is in contact adsorption with a gas mixture containing acetylene and ethylene, and efficient separation of acetylene / ethylene can be realized at normal temperature, low acetylene content and low acetylene partial pressure.

The invention discloses a high-specificity sulfanilamide imprinting quantum dot fluorescent sensor. The high-specificity sulfanilamide imprinting quantum dot fluorescent sensor is prepared by taking an amino and vinyl modified silicon dioxide microsphere as a carrier, an octadecyl-p-vinylbenzyl-dimethyl ammonium chloride modified cadmium telluride quantum dot as a fluorescent material, sulfanilamide as a template molecule, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent and 2,2-azobisisobutyronitrile as an initiator through a precipitation polymerization method. Furthermore, the invention further discloses a preparation method of the high-specificity sulfanilamide imprinting quantum dot fluorescent sensor. The high-specificity sulfanilamide imprinting quantum dot fluorescent sensor disclosed by the invention has good optical stability and high specificity and high sensitivity on the sulfanilamide, and can be used for rapidly identifying and detecting sulfonamide antibiotics on the scene; meanwhile, the preparation method is simple and convenient, low in equipment requirement and easy to realize.

The invention discloses a method for detecting pathogenic bacteria in foods by isothermal rapid amplification and belongs to the technical field of food safety detection. The method comprises the following steps: determining detection target nucleic acid aiming at each bacterium through multiple sequences; designing a specific molecular beacon probe and a primer; taking a genome DNA (Deoxyribonucleic Acid) of each strain as a template and establishing real-time fluorescence CSDPR; carrying out the isothermal nucleic acid amplification and identifying the specificity, sensitivity and minimum limit of detection of a CSDPR method. The detection of an actual sample determines that the method established by the invention has practical feasibility. The method disclosed by the invention has commonality on detection of various pathogenic bacteria in the foods and the pathogenic bacteria comprise staphylococcus aureus, salmonella, enterohemorrhagic escherichia coli, shigella and the like; only if a specific part in the molecular beacon probe needs to be replaced, effective detection of the different pathogenic bacteria can be realized; and the method has high detection sensitivity and high specificity, simple principle and operation and the pretreatment of the sample is simple.

The invention, which belongs to the field of environmental monitoring and electrochemical sensors, relates to a tetrabromobisphenol A molecularly-imprinted electrochemical sensor, and a preparation method and application thereof. A glassy carbon electrode modifying a multi-walled carbon nanotube and gold nanoparticles is used as a working electrode; a polymer film of polypyrrole and tetrabromobisphenol A is prepared by using a cyclic voltammetry method; and then tetrabromobisphenol A is eluted to obtain a molecularly-imprinted electrochemical sensor for direct electrochemical detection of tetrabromobisphenol A. Compared with the traditional detection method, the provided method has characteristics of convenient operation, high sensitivity, high specific recognition ability, high reproducibility and strong anti-interference ability.

Belonging to the technical field of functional materials, the invention relates to a Janus magnetic molecularly imprinted nanosheet, a preparation method and application thereof. The method includes:preparing Janus hollow microspheres by sol-gel method, and conducting ultrasonic breaking to obtain a Janus nanosheet containing amino on one side and containing chlorine element on the other side; then taking surface chlorine element as the initiator of atom transfer radical polymerization, adopting dA as the template molecule, using 5-(2-methoxy vinyl)-2'-deoxyuridine as the functional monomer,and grafting a dA molecularly imprinted polymer to the hydrophobic surface of the Janus nanosheet; and then combining oleic acid coated Fe3O4 particles to the hydrophobic surface of Janus through thebonding effect between amino and carboxyl to prepare a Janus magnetic molecularly imprinted nanosheet adsorbent, and applying the obtained material to adsorption and separation of dA. The imprinted adsorption material constructed by the invention has the advantages of good selectivity, large adsorptive capacity to target dA and rapid separation.

The invention discloses a method for preparing a magnetic halloysite molecularly imprinted polymer with specific adsorption to 2,4-dichlorophenoxyacetic acid. The preparation method comprises the following steps: preparing magnetic halloysite nano-tubes by using corroded halloysite nano-tubes by a coprecipitation method; reacting the magnetic halloysite nano-tubes and methacrylate in methylbenzene to obtain magnetic halloysite nano-tubes with double-bond-modified surfaces; after polymerizing the magnetic halloysite nano-tubes with double-bond-modified surfaces, the polymerized monomers and a crosslinker by taking the 2,4-dichlorophenoxyacetic acid as a template, then removing the 2,4-dichlorophenoxyacetic acid to obtain the magnetic halloysite molecularly imprinted polymer with specific adsorption to the 2,4-dichlorophenoxyacetic acid. The raw materials used in the preparation method are low in cost; the preparation method is easy to operate and easy to implement; the prepared magnetic halloysite molecularly imprinted polymer is stable in structure, has specific adsorption to the 2,4-dichlorophenoxyacetic acid, and can effectively separate and remove the 2,4-dichlorophenoxyacetic acid from a complex system.

The invention discloses a piezoelectric detection sensor for microcystic toxins based on a molecular imprinting technology. The piezoelectric detection sensor is characterized in that a photochemistry polymerization method or an electrochemical polymerization method is utilized to compound a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is shortened, and the specific adsorption capacity is strengthened; meanwhile, a quartz crystal serves as an effective apparatus of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, and the cost is reduced. The piezoelectric detection sensor provided by the invention has the advantages that the property of the sensor is improved, the trace detection of the microcystic toxins is realized, and the microcystic toxins detection method which is high in sensitivity, high in speed, low in cost, and low in operation requirement is established.

The invention discloses an electrochemical sensor for detecting nitrite, a preparation method and application, and belongs to the field of electrochemical analysis. The preparation method comprises the following steps of: etching aluminum carbide to obtain titanium carbide with of accordion-like structure, calcining the titanium carbide serving as a titanium source to obtain titanium dioxide-titanium carbide, dispersing the compound into a solution containing hexadecyl trimethyl ammonium bromide and chitosan, and performing ultrasonic treatment to obtain a composite solution. According to theelectrochemical sensor for detecting nitrite, the preparation method and the application, a three-electrode system is adopted, a glassy carbon electrode modified by a titanium dioxide titanium carbide / hexadecyl trimethyl ammonium bromide / chitosan compound is used as a working electrode, and the nitrite is determined by differential pulse voltammetry. The electrochemical sensor disclosed by the invention has the advantages of high sensitivity, good selectivity, low detection limit, quick response and the like in nitrite detection, and has a wide application prospect in the aspects of environment and food detection.

The invention relates to a method and reagent for immunological detection of a homogeneous enzyme determination technology, and is used for determination of the ADMA (N<G>,N<G>,dimethyl-L-arginine, asymmetric dimethylarginine, ADMA) content in a detected sample. According to the invention, anti-ADMA specific antibodies and enzyme-ADMA complexes are used, the immunological reaction of the enzyme-ADMA complexes and the anti-ADMA specific antibodies is utilized, after antigen antibody complexes are formed, the activity of the enzyme-ADMA complexes is inhibited, the changes of enzymatic activity in a reaction system is determined to obtain the number of combined enzyme markers, so as to obtain the content of ADMA in the detected sample. The method has the advantage of capability of realizing immunoassay as the common biochemical enzyme method, so as to simultaneously determine multiple samples on a full-automatic biochemical analyzer.

The invention relates to a multi-hydrogen-bond melamine core-shell molecularly imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: by adopting SiO2 as an inner core, performing modification on the surfaces of SiO2 nano particles by using 3-aminopropyltriethoxysilane and glutaraldehyde, and connecting a functional monomer 6-aminouracil; and performing polymerization reaction on melamine as a template molecule, methacrylic acid, an initiating agent and a crosslinking agent to prepare the multi-hydrogen-bond melamine core-shell molecularly imprinted polymer. The multi-hydrogen-bond melamine core-shell molecularly imprinted polymer disclosed by the invention can be used for exchanging the roles of a functional monomer in a conventional three-hydrogen-bond molecularly imprinted system and the template molecule, adopts the functional monomer in the conventional system as the template molecule, adopts a template molecule in the conventional system as the functional monomer in a system disclosed by the invention, has very high selectivity, has specific binding only aiming at melamine and triazine compounds, and has good selective enrichment and adsorption effects in highly-specific food detection.

The invention discloses a preparation method of a chlorogenic acid adsorption material. The preparation method comprises the following steps: firstly, adding a pore-foaming agent into a reactor; secondly, sequentially adding a template molecule, a functional monomer, a crosslinking agent and an initiator and reacting to obtain a hard solid; grinding the hard solid into powder, and carrying out soxhlet extraction and vacuum drying. The chlorogenic acid adsorption material prepared by the application has the advantages of high selectivity, high adsorption rate and high adsorption capacity on chlorogenic acid, capability of being applied to adsorption, separation and purification of the chlorogenic acid in a complex system and broad application prospect in the enrichment, separation and purification of the chlorogenic acid; the preparation method has the advantages of simplicity, easiness in operation and lower cost.

The invention discloses a nanocage probe, an application thereof and a nucleic acid detection method, the nanocage probe comprises gold / silver nanocage particles, the surfaces of the particles are modified with hairpin DNA chains, and the hairpin DNA chains are partially complementary with nucleic acid to be detected; gold / silver nano cage particles are used as a nucleic acid recognition reactionsubstrate and a scattering signal indication probe; a hairpin chain is modified on the surface of the probe as a nucleic acid recognition unit; DNA circulation and HCR amplification processes are realized by utilizing DNA base complementary pairing and strand substitution reaction rules, so that G-quadruplex-heme DNA enzyme is formed on the surface of the probe, etching of a silver component in the nanocage is realized by virtue of active oxygen generated by enzymatic catalysis of hydrogen peroxide decomposition, and a light-off response of a dark field signal is caused. The dark field signalchange with the time resolution capability shows the dependence on the concentration of the nucleic acid to be detected, and quantitative analysis is carried out in combination with a statistical analysis method.

The invention discloses preparation of a functionalized three-dimensional graphene composite material and an application technology of the functionalized three-dimensional graphene composite materialin chemiluminescence sensors. The technology is mainly characterized in that a beta-cyclodextrin / ionic liquid@graphene aerogel is prepared, and the surface of the beta-cyclodextrin / ionic liquid@graphene aerogel is modified with an aptamer to obtain a functionalized three-dimensional graphene composite material with high specific recognition ability to streptomycin molecules, wherein the method hascharacteristics of simple preparation process, easily-controlled conditions and low production cost. The present invention further provides a new method for detecting streptomycin. According to the present invention, with the application of the aptamer functionalized beta-cyclodextrin / ionic liquid@graphene aerogel in the chemiluminescence sensor for detecting streptomycin, the advantages of highsensitivity, good selectivity, convenient operation, simple instrument and the like can be achieved; and the functionalized three-dimensional graphene composite material is successfully used in the detection of streptomycin in cucumber samples, has high accuracy and high precision, provides the possibility in actual detection, and has important biological significance in food safety and human health.

The invention discloses a method for visually detecting genetically modified foods, and belongs to the field of food safety. The method is characterized in that the genetically modified foods are treated as detecting objects, and the genome DNA of the genetically modified foods is extracted; a special CaMV 35S nucleic acid probe capable of specifically recognizing the genetically modified foods and a corresponding template sequence are designed; a sequence complementation template sequence is combined to form a ring in the presence of a specific CaMV 35S promoter; the rolling circle amplification reaction is triggered under the effects of T4-DNA ligase and phi 29 polymerase; G-four chain body in the amplification product is combined with oxidized heme to generate DNA enzyme for catalyzingABTS developing, thus visually detecting the genetically modified foods. With the adoption of the method, the promoter CaMV 35S is visually detected in the genetically modified foods; the method has the advantages of being simple to operate, and visually detecting; and the method is applicable to detection of genetically modified soybean, corn, tomato and other genetically modified foods containing the CaMV 35S promoter genes.

The invention belongs to the technical field of molecularly imprinted polymers, and particularly relates to a preparation method of protein affinity blotting aquagel polymers based on acrylamide groupmetal chelation monomers. The invention aims at providing the preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers by aiming atthe technical analysis and the existing problems. The preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers has the technical scheme that according to the method, after the acrylamide group monomers are chelated with divalent metal ions, target protein is subjected to specific adsorption combination and removal; then, polymers are obtained. The preparation method has the following characteristics that the acrylamide group monomers have the metal chelation capability; the molecular mark aquagel has the temperature and pH duplex sensitivity; the reverse phase suspension technology is used; the polymers are aquagel polymers.

The invention provides a preparation method and application of a molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually. According to the preparationmethod, two types of quantum dots are used as fluorescence signal sources, functional monomers and hydrophilic monomers are added simultaneously, HAV and HBV are used as templates to be imprinted on different carriers respectively, obtained imprinted polymers are mixed after the templates are eluted, and the fluorescence sensor capable of detecting the two types of viruses simultaneously is obtained. Through the sensor, the advantages of the hydrophilic monomers N-isopropyl acrylamide (NIPAAm) and the advantages of the functional monomers zinc acrylate are combined simultaneously, and high-specificity recognition of the two types of viruses HAV and HBV is realized. Since the quantum dots have different fluorescence quenching degrees after being combined with target objects with different concentrations, visual detection is realized. The provided strategy lays a good foundation for simultaneously detecting multiple similar viruses through virus molecule imprinting and meanwhile has a potential ability in clinical treatment and diagnosis of viral diseases.

The invention relates to a preparation method of a fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles. The fluorescent nanoparticles are taken as a carrier, and linear nonapeptide and adriamycin of an extracellular region where HER2 is over expressed are used as double templates to synthesize the imprinted polymer. The method comprises the followingsteps: preparing fluorescent silicon nanoparticles (Si NPs), carrying out silanization on the Si NPs; and preparing the fluorescent double-template antigenic-determinant imprinted polymer based on thesilicon nanoparticles. According to the method, the imprinted polymer is synthesized by combining antigenic-determinant imprinting, double-template imprinting and a surface imprinting method, the operation is simple and convenient, the process saves time, and the material has a targeting imaging function and a targeted therapy function. Zinc acrylate and acrylamide are adopted as functional monomers, and the templates are jointly fixed through metal chelating and action of hydrogen bonds, so that the specific recognition effect of the material on a target object can be effectively improved, and controlled release of medicines can be realized by utilizing an acidic pH value. The synthesized fluorescent imprinted polymer efficiently combines high sensitivity of fluorescence and high selectivity of a molecular imprinting technology.

The invention discloses an indicator diagram fault diagnosis method based on a generative adversarial neural network, and belongs to the technical field of oil extraction fault diagnosis, and the method comprises the following steps: carrying out the data cleaning of indicator diagram sample library data; based on an oil production engineering theory and typical indicator diagram characteristics, performing feature extraction on indicator diagram data points; adopting a generative adversarial neural network to generate a small number of fault category samples, and performing conditional constraint on the output of a generator network in the generation process; dividing the data into a training set, a verification set and a test set based on the original sample and the generated sample; an Xgboost classification algorithm is adopted to classify the samples; comprehensively evaluating a fault diagnosis result by utilizing the accuracy rate and the recall rate; and performing real-time monitoring and diagnosis on the fault by using the trained classification model, and judging the fault type in real time. The method can significantly improve the specific recognition capability of the classification model for the fault sample, and reduces the false alarm / missing report rate of the fault.

The invention discloses a method for immunological detection of a homogeneous enzyme assay technology and a reagent thereof. The method is used for assaying the content of a vitamin B12 (Vitamin B12,VB12) in a sample. The method is capable of adopting an anti-VB12 specific antibody and an enzyme-VB12 compound, and using an immunology reaction of the enzyme-VB12 compound and the anti-VB12 specificantibody, after forming an antigen-antibody compound, inhibiting the activity of the enzyme-VB12 compound, and through assaying the change of the enzymatic activity in a reaction system, acquiring the number of combined enzyme markers, thereby acquiring the content of the VB12 in the sample to be tested. The advantage of the method is that the immunology assay can be realized as a general biochemistry enzyme method, so that multiple examples are simultaneously assayed in a full-automatic biochemical analyzer.

The invention discloses a detection method for malachite green in aquatic products. The detection method comprises experiment steps of preparing a magnetic molecularly imprinted polymer, establishinga method based on an aptamer and ferroferric oxide mimic enzyme catalysis and the like. According to the detection method, firstly, a dopamine polymerization method is used for preparing a malachite green (MG) magnetic molecularly imprinted polymer (MG-MMIPs); trace MG in a sample is adsorbed by utilizing the MG-MMIPs, so that the aims of enriching a target object and removing a complicated mediumare realized; then after enriched MG is desorbed, a detection method of the aptamer and ferroferric oxide mimic enzyme catalysis is used for detecting. The method has the sensitivity of 5 nmol L<-1>and the lowest detection limit reaches 3.6 nmol L<-1>. A specific experiment aiming at two structural analogues (including brilliant green and crystal violet) of MG and other bacteriostatic drugs (including sulfaguanidine, sulphanilamide, oxytetracycline hydrochloride and chloramphenicol) shows that the method has good selectivity in MG detection.

The invention provides a preparation method of a molecular imprinting ratio type fluorescence sensor for detecting hepatitis B virus. According to the preparation method, a metal organic framework material (MIL-101) is taken as an imprinting carrier, the surface of the imprinting carrier is modified with a virus aptamer, a fluorescent dye monomer rhodamine B and a template virus are added for imprinting, and the molecularly imprinted polymer with aptamer and molecularly imprinted cavity dual recognition is obtained after a template is eluted. After the imprinted polymer is combined with a target object, the fluorescence intensity of a fluorescent dye rhodamine B is changed, and the fluorescence of MIL-101 is almost unchanged, so that a ratio type fluorescence detection system is constructed. According to the method, the advantages of a metal organic framework material, a molecular imprinting technology and fluorescence ratio type detection are combined, the detection sensitivity is high, the detection limit is low, the selectivity is good, the background signal is low, the imprinting factor is as high as 5.72, and the method has a good application prospect in the aspect of virus detection.

The invention relates to a microcystic toxin molecular imprinting functionalization TiO2@CNTs photoelectric sensor electrode and a photoelectric analysis method with the same. The electrode is composed of a substrate electrode and an MI-TiO2@CNTs nano-material covering the surface of the substrate electrode. The MI-TiO2@CNTs nano-material is composed of a multiwalled carbon nanotube and a TiO2 nano-particle layer, wherein the TiO2 nano-particle layer wraps the outer surface of the multiwalled carbon nanotube and is provided with MC-LR in-situ molecular imprinting sites. Compared with the prior art, the electrode greatly improves the photoelectric catalytic performance, and is good in selective recognition performance and adsorption enrichment performance for MC-LR and high in photoelectric detection sensitivity, and the limit of detection reaches 1.0*(10-12) mol.L-1; meanwhile, a preparation method is simple, an analysis method is quick and simple, good repeatability is achieved, and the method can be applied to detection analysis on trace contaminants.

The invention belongs to the field of immunological detection, and relates to a kit, in particular to a kit for rapidly detecting peripheral T cell lymphoma and a use method of the kit. The kit comprises a reagent I and a reagent II, the reagent I comprises two groups of monoclonal antibody mixtures, and the first group of monoclonal antibody mixture comprises TRBC1, CD2, CD3, CD5, CD7, CD16, CD56, CD57 and CD45; the second group of monoclonal antibody mixture comprises the following components: TRBC1, TCR-gamma delta, CD3, CD8, CD4, CD7 and CD45; and the reagent II is hemolysin. The kit provided by the invention realizes the rapid and accurate detection of the peripheral T cell lymphoma (PTCL), is simple and convenient, greatly reduces the requirement on the sample size, and reduces the burden of a patient.

The invention relates to the field of organic high-polymer materials, in particular to a tetrahydroxyldiphenylethylene-2-o-glucoside adsorbing material and a preparation method thereof. Tetrahydroxyldiphenylethylene-2-o-glucoside is used as a template molecule, substituent 2-amino ethyl methacrylate is used as functional monomer, the template molecule and the functional monomer are in cooperationwith a cross-linking agent, an initiator and a pore-foaming agent to prepare a three-dimensional hole polymer highly matched with the tetrahydroxyldiphenylethylene-2-o-glucoside molecule, the preparedadsorbing material is an adsorbing material which has high selectivity and high adsorption capability for the tetrahydroxyldiphenylethylene-2-o-glucoside, and can be applied to adsorbing, separatingand extracting the tetrahydroxyldiphenylethylene-2-o-glucoside in a complex system, and a preparation technology is simple.