82results about How to "Individual small" patented technology

An extraction method of sea cucumber polypeptide contains steps of: embathing, high-pressure stewing, enzymatic hydrolysis, deodorizing, filtering, condensation, drying and finished product packaging. The extraction method is characterized in that high-pressure stewing: the temperature is 120-130 DEG C, the pressure is 0.13-0.15 MPa and stewing lasts for 2 hours at constant temperature and constant pressure; enzymatic hydrolysis: the temperature is cooled to 54-56 DEG C after heating and sterilizing, NaOH is added to adjust PH to 7.0 and 0.5% of sea cucumber special-purpose enzyme to perform enzymatic hydrolysis for 6 hours; deodorizing: 17% of active carbon is added for deodorizing; filtering: a standing liquid firstly passes through a candle filter, then passes through a barrel filter and is finally filtered to a clear liquid storage tank; and condensation: condensation pressure is minus 0.04-minus 0.06 MPa, condensation temperature is 75-80 DEG C, and Baume degree determined during the condensation process is controlled at 10. The invention has the following advantages of: sea cucumber is changed into a fully water soluble active component after biological enzyme hydrolysis; the content of small peptides in the product is high, which is more beneficial to the absorption and utilization of the sea cucumber active component; and sea cucumber polypeptide is more acceptable in taste after deodorizing by active carbon. The invention is suitable for the extraction method of sea cucumber polypeptide.

The invention discloses a method for comprehensively utilizing euphausia superba shells. The method is a comprehensive utilizing method, wherein the shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases of euphausia superba with high activity based on euphausia superba shells as raw materials to recover proteins and astaxanthin, while residues are used for producing chitin. The shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases, so that the protein recovery rate is effectively increased. Enzymatic hydrolysates are filtered and centrifugalized, and the filtrate is concentrated to obtain protein concentrate or sprayed and dried to obtain soluble protein powder. The protein content in the protein powder is 75-85%. Precipitates are freezed and dried to obtain the protein powder rich in astaxanthin, wherein the protein content is 50-60% while the astaxanthin content is 600-900mg / kg. The addition amount of exogenous enzymes is reduced by 40-50% by replacing part of exogenous enzymes by enzymolysis of endogenous proteinases in a way of combining the characteristic that the endogenous proteinases of the euphausia superba are high in activity, so that the cost is greatly lowered.

A method for in-lab breeding of Spodoptera frugiperda in winter comprises the steps of (S1) adult pairing, (S2) uniform egg cultivation, (S3) incubation and feeding, (S4) excrement cleaning and feed changing for first to third larvae, (S5) dividing and breeding of advanced-age larvae, (S6) preparation of a pupation environment, and (S7) centralized pupa breeding and worm formation by pupation. Different devices are used in different stages, disposable plastic cups are used during adult pairing and larva breeding, narrow space of the disposable plastic cups is beneficial for adult pairing, andescape of young-age larvae which are small in size and quick in creeping can be prevented through cup breeding; and a latticed breeding device is used for the advanced-age larva, so that mutual bitingcaused by competition can be avoided, space is saved, and the larvae of different ages can be bred at the same time. The method for in-lab breeding of Spodoptera frugiperda in winter effectively increases a survival rate, is high in breeding density and small in floor area, increases the yield, is convenient to operate and saves time and effort.

The invention relates to an acete chinensis trap net capable of releasing rhopilema esculentum and an assembling method thereof, and belongs to the technical field of marine fishing. Under the circumstance of not changing the performance of catching acete chinensis, rhopilema esculentum blocking and separating net pieces are obliquely and additionally installed in a main net in a single body tapercapsular bag shape to form a net-within-net structure, and a rhopilema esculentum releasing opening is reserved in the back middle position of an upper side gap of a main netting in the single body taper capsular bag shape. The suitable integral floating and sinking ratio is selected, a big floating ball is additionally installed in the net, and by adjusting the length of a big floating ball outline, aimed fishing is conducted. The trap net is simple in structure, easy to manufacture, convenient to operate and low in cost; the rhopilema esculentum can be effectively released, and the fishingperformance of the acete chinensis trap net cannot be influenced.

The invention provides a wastewater treatment system. The wastewater treatment system is characterized by comprising a primary grating, a secondary grating, a fiber rotating disc filter tank, an acid-base balance tank, a three-stage internal aeration membrane device, a secondary sedimentation tank and a disinfection and monitoring tank which are connected through pipelines sequentially, wherein asludge return pipe is arranged between a sludge outlet of the secondary sedimentation tank and a sewage inlet of the acid-base balance tank; a reflux pump is mounted at the sludge outlet of the secondary sedimentation tank; a sludge outlet of the acid-base balance tank and a floating slag outlet of the secondary sedimentation tank are connected to a sludge concentration tank which is connected with a dewaterer; grid bar clearance of the primary grating is larger than that of the secondary grating. The industrial wastewater treatment system which is simple in structure, good in sewage treatmenteffect and high in efficiency is provided.

A method for ecologically cultivating rotifers in a summer eastern puffer fish fingerling culture pond includes fingerling breeding, feed introduction and daily feeding, rotifer introduction, aeration, recovery, water change and repeated cultivation. The method is characterized in that outdoor earth ponds are used for breeding fingerlings, each pond is provided with an aerator, water is injected into the ponds after the ponds are cleaned and disinfected before the fingerlings are bred, summer fingerlings are bred one week after the water is injected, feed introduction is started after the summer fingerlings are bred for 2-3 days, the fingerlings are conventionally cultivated after domestication for 7 days, the rotifers in allogenic water are introduced by water change, the aerators need to be started after water change and the introduction, recovery can be performed after the rotifers are introduced for 5-7 days, a 4-inch submersible pump and a 150-200-mesh net bag are used for suction filtration in recovery, a 16-20-mesh net bag basket sleeves a water pump, suction filtration is performed for 20-30 minutes every time and for 2-3 times every day, the extraction amount and the reproduction amount are kept balanced, recovery is continued for 5-7 days, the water is substantially changed by 2 / 3 to improve water quality, and the rotifers are repeatedly cultivated once one month later.

The invention discloses a method for selectively breeding bivalve shellfish at an attachment stage, which comprises the following steps of: putting synchronous fertilized eggs of the bivalve shellfish into a nursery pond to perform culture till the fertilized eggs are grown to a veliger stage; when 30 to 40 percent of larvae have eye spots, putting attachment matrixes into the nursery pond; determining the times and amount of fed baits every day according to the condition of larva stomachs under a microscope; changing the water once to twice every day; when the attachment rate of the larvae is 10 to 30 percent, taking the attachment matrixes attached with the larvae out to perform separate culture; and breeding the larvae taken out in the previous step at a reduced temperature, reducing the temperature by 1 to 2 DEG C every day till the temperature is reduced to the temperature of natural seawater, and directly culturing the larvae on the sea. The invention provides the efficient and large-scale selective breeding method, which solves the problems of small basic population scale of selectively breeding the bivalve shellfish, high selective breeding cost, difficult process operation, single selective breeding target and the like.

The invention provides a factory-like full-artificial propagation method of sebastes schlegeli. The method comprises the following steps of 1) factory parent fish optimization and cultivation; 2) artificial insemination; 3) parent fish breeding after insemination; 4) parent fish pond arrangement and farrowing; and 5), fry rearing. According to the factory-like full-artificial propagation method ofthe sebastes schlegeli, the insemination success rate of parent fishes reaches 88.5% or above, the farrowing survival rate reaches 78.6% or above, the fry survival rate reaches 48.0% or above, high-quality fries with the average full length of 5.2 cm can be obtained after 2.5 months of cultivation, and the propagation efficiency of female parent fishes of the sebastes schlegeli is effectively improved; and the large-scale mass production of high-quality offspring seeds is broken through, the dependence of directly fishing parent fishes with offspring on wild resources and cage culture parentfish groups is solved, the problems of low factory mating rate, low offspring survival rate and the like are solved, and the method has higher popularization value.

The invention relates to the technical field of detection, and specifically relates to a detection method for the sunscreen efficacy of sunscreen skin care products. The detection method takes zebrafish as an experimental carrier, and evaluates the sunscreen efficacy of sunscreen skin care products by comparing the completeness of zebrafish fins before and after ultraviolet radiation. The detection method is short in experiment period and low in cost, and can scientifically and effectively evaluate the sunscreen efficacy of the sunscreen skin care products.

The invention discloses a method for establishing a Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof. According to a concrete technical scheme in the invention, the method comprises the following steps: preparing an emulsion (an MOG solution: CFA = 1: 1) from MOG 34-56 (100 mu g / ml); narcotizing Macaca fascicularis for experiments and injecting 1 ml of the prepared emulsion at 10 injection points; carrying out immunization injection of the emulsion (secondary immunization) according to the above-mentioned method and dose in 7 days after the primary immunization injection (primary immunization); carrying out clinical observation in one day after primary immunization and recording clinical scores; and determining pathogenic sites, degrees and the like by using an MRI iconographic method at pathogenic time nodes. The model established in the invention has an application value which cannot be achieved by other rodent models, has the characteristics of recurrence-alleviation type attacks, low cost, wide availability of Macaca fascicularis for experiments and the like compared with a Macaca rhesus model and has a wide application scope in fields related to multiple sclerosis diseases.

The invention relates to a biological behavior biosensor, which is characterized by comprising two series connected cavities, wherein a screen is arranged between the two cavities; outer ends of the two cavities are fixedly provided with a round cap respectively; the center of each round cap is provided with a water pipe connector; at least one group of electrodes is arranged in each cavity; each group of electrodes is formed by two pairs of electrodes which are arranged oppositely; one pair of electrodes is emission electrodes, and the other pair of electrodes is signal receiving electrodes; and the two pairs of electrodes are respectively connected with a water quality safety on-line warning system. The biological behavior biosensor provided by the invention is applied to the water quality safety on-line monitoring and warning system and is used for monitoring dynamic water, when in detection, a tested organism is put in one cavity while nothing is put in the other cavity, and a signal difference sensed by the two cavities is adopted as the behavior change of the tested organism, so that the influence of an external environment is removed, the behavior change of the tested organism in the environment is furthest monitored in real time, a biological behavior signal with normal frequency as well as high frequency and low intensity can be monitored, and the detection accuracy is improved.

The invention belongs to the technical field of biology, and discloses application of glutamate dehydrogenase as a target spot in pest control. The invention discloses the application of glutamate dehydrogenase as a target spot in pest control for the first time, GDH is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of glutamate dehydrogenase to growth and development of pests is proved. GDH gene mutants are successfully obtained in pests by utilizing a transgenic CRISPR / Cas9 technology, GDH gene mutation seriously affects normal growth and development of the pests, individuals become small, and the pests are prone to being infected with viruses in a larval stage and die, and massive death caused by GDH gene mutation in the larval stage can be used for pest control.

The invention discloses a special primer combination for quickly detecting polyploid of a willow tree and a detection method for the polyploidy. A primer provided by the invention is used for performing PCR amplification on a DNA template of the to-be-detected willow tree; by means of electrophoresis detection of an amplification product, if the number of alleles detected on amplification sites of any pair of primers of the detected sample is greater than 2, the sample is the polyploid. Compared with the existing willow tree polyploid detection method, the special primer combination which is provided by the invention and is used for willow tree polyploid screening has the advantages of simple and quick operation method and reliable result, can detect a large quantity of samples within short time, and supplies efficient and reliable technical measures to willow tree polyploid screening. An important technical support is supplied to willow tree polyploid cultivation; the special primer combination has extremely high actual application values, can achieve relatively high economic and social benefits, and has wide application prospects.

The invention discloses a method for establishing a non-human primate autoimmune cerebrospinal meningitis model and the application of the model. The specific technical scheme comprises the following steps; a machin is selected as the non-human primate experimental animals; MOG34-56(100Mug / ml) is prepared into an emulsion (MOG CFA=1; 1); after the experimental machin is anesthetized, the machin is intradermally injected with 1ml of prepared emulsion at 10 injection points; on the seventh day after the first injection, an MOG-CFA emulsion is prepared by use of the same method, and the second immune injection is performed at the same dosage and by use of the same method; the experimental autoimmune cerebrospinal meningitis model established by use of the method has the clinical characters of remission-relapse, and can be widely used to the related field of disseminated sclerosis diseases. Compared with the existing rhesus method and model, the method and the model also have the characteristics of short induction period, low cost, rich monkey resources and the like, and also have the application value which cannot be realized by rodent models.

The invention discloses a detection system for proportional valves or servo valves. The detection system comprises a proportional valve / servo valve detector, wherein the proportional valve / servo valve detector can output a standard analog signal or direct driving current for a proportional valve / servo valve electromagnet, and has standard analog signal output and detection functions and a function of outputting direct current for a proportional electromagnet or a servo electromagnet; the proportional valve / servo valve detector comprises a main control chip, a driving current module and a power processing module; and the main control chip gives a test instruction to the tested proportional valve / servo valve, and receives valve core signals of the tested proportional valve / servo valve. The detection system has the characteristics of comprehensive test range, complete function, convenience in use, simple structure of an operation interface, high test and record precision, high reliability and safety and the like, and is applicable to detection and analysis of functions and properties of all the proportional valves or servo valves of a hydraulic system.

The invention relates to a gear speed changing box, in particular to a speed changing box of a gear. The gear is formed by laminating a plurality of quenched sheet parts with teeth, and gaps of the gear are filled with a metal solution. The speed changing box comprises a box body, a shaft and the gear, the shaft is installed in the box body, and the gear is arranged on the shaft. The gear speed changing box is characterized in that the gear is a multilayer gear; the gear comprises a gear body, and the gear body is formed by laminating the multiple quenched sheet parts with the teeth; at least one connecting hole is formed in each sheet part, the connecting holes are laminated to form a through hole after the sheet parts are laminated, and the connecting holes are fixedly connected into the gear body through connecting pieces; and then the gear body is soaked into a metal solution, the gear body is taken out from the metal solution to be cooled, the metal solution solidifies into metal to be injected into the positions between the sheet parts of the gear body and the surface of the gear body, and the technical aim of rapidly manufacturing the gear with the high physical property in a low-cost manner is achieved.

The invention relates to a multilayer gear. In order to overcome the technical defects that in the prior art, the gear machining process is complex, the machining time is long, the machining difficulty is large, and the machining cost is high, the gear is provided. The gear comprises a gear body which is formed by laminating a plurality of quenched sheet parts with teeth, at least one connecting hole is formed in each sheet part, the connecting holes are laminated to form a through hole after the sheet parts are laminated, and the connecting holes are fixedly connected into the gear body through connecting pieces; then the gear body is soaked into a metal solution; the gear body is taken out from the metal solution to be cooled; and the metal solution solidifies into metal to be injected into the positions between the sheet parts of the gear body and the surface of the gear body, and the technical aim of rapidly manufacturing the gear with the high physical property in a low-cost manner is achieved.

The invention is suitable for the technical field of lubricating oil, and provides lubricating oil and a preparation method thereof. The lubricating oil is prepared from the following raw materials in percentage by weight: 18-25% of polyol ester, 8-10% of alkyl naphthalene, 26.3-40.75% of an additive, and the balance of poly alpha olefin. The lubricating oil disclosed by the invention is more stable in wear resistance, and the wear resistance of the lubricating oil disclosed by the invention exceeds 15% of the ACEA E6 standard requirement; the lubricating oil provided by the invention can better reduce the formation of sediments, and can provide excellent engine cleaning performance which exceeds 36% of the API CI-4 standard requirement; the viscosity stability is high, and the viscosity stability performance exceeds 8 times of the APICI-4 standard requirement; the raw materials of the lubricating oil do not contain corrosive substances, the lubricating oil does not harm the environment or damage mechanical equipment, and the lubricating oil is non-flammable and wide in application range.

The invention discloses an efficient cultivation system and method for mudflat economic shellfish juvenile mollusks. A 5-8 mu algae species pond and a 20+ / -2 mu algae enlarged culture pond is constructed, seawater and algae species therein are introduced, and algal facies of the seawater in the algae species pond is observed, thereby facilitating disinfection treatment and fertilization of the seawater in the pond. After microscopically detecting the algal facies, proper algal facies are selected, directional enlarged culture on high-quality algae is conducted, water is fertilized to quickly breed the algae to a certain scale, 1 / 3 of seawater reaching the specified enlarged culture concentration in the algae species pond is introduced into the enlarged culture pond for enlarged culture, water is added again to reach the enlarged culture density, pond moving operation is repeated for continuous culture, and finally, water in the enlarged culture pond is led into a seedling raising chamber for juvenile mollusk running water circulation feeding culture, so as to promote growth and development of shellfish. According to the method disclosed by the invention, about 10 billion seedlings with the specification of 1,600,000 / kg can be cultured in the seedling raising chamber with the culture scale of 4-5,000 square meters, so that the culture cost is saved.

The invention provides aloe facial cleanser which is prepared by raw materials in parts by weight as follows: 2-4 parts of aloe extracts, 4-6 parts of geniposide, 3-5 parts of puerarin, 5-7 parts of acetyltalatisamine achyranthan, 20-30 parts of hexadecyl(sulfophenoxy) benzenesulfonic acid, disodium salt, 10-15 parts of coconut monoethanol amide, 5-7 parts of caprylic capric triglyceride and 150-180 parts of distilled water. The aloe facial cleanser has fine and stable foam, is mild in property, stable in viscosity and convenient to use, has low stimulation on skin, good environmental protection property and good use effect, and the aloe facial cleanser can have a better skin whitening effect after other extracts are added particularly.

The invention provides aloe facial cleanser which is prepared by raw materials in parts by weight as follows: 2-4 parts of aloe extracts, 4-6 parts of geniposide, 3-5 parts of puerarin, 5-7 parts of acetyltalatisamine achyranthan, 20-30 parts of hexadecyl(sulfophenoxy) benzenesulfonic acid, disodium salt, 10-15 parts of coconut monoethanol amide, 5-7 parts of caprylic capric triglyceride and 150-180 parts of distilled water. The aloe facial cleanser has fine and stable foam, is mild in property, stable in viscosity and convenient to use, has low stimulation on skin, good environmental protection property and good use effect, and the aloe facial cleanser can have a better skin whitening effect after other extracts are added particularly.

The present invention relates to a whitebait processing method. The method comprises the following steps: (1) first mixing water, potassium sorbate, d-sodium erythorbate and calcium propionate to prepare a mixed solution one; (2) mixing flour, salt, sugar and ginger powder to prepare a mixed substance two; and (3) soaking the whitebait in the mixed solution one for 15-20 seconds, then taking out, drying, placing the whitebait into the mixed substance two to evenly wrap the whitebait with the mixed substance two, removing redundant mixed substance two, and then arranging the whitebait and carrying out individual quick freezing. The whitebait processed by the invention meets requirements on whitebait sterilization, and has a shelf life 6-12 months longer than the whitebait processed by simple freezing.

The invention provides a compressor for a combined screw rod and particularly relates to a compressor for a combined screw rod shaft. A screw rod shaft body is formed in the mode that a plurality of quenched sheet parts with teeth are arranged on a shaft in a sleeving mode, stacked after being glued and spot-welded. The compressor comprises a cylinder, and the screw rod shaft is arranged in the cylinder. The screw rod shaft is a sheet part combined screw rod shaft. The screw rod shaft further comprises the sheet parts and the shaft. The outer side of each sheet part is provided with a tooth bottom engagement part and a tooth top engagement part. A shaft hole is formed in each sheet part, at least one tooth-shaped protrusion is arranged in each shaft hole, and at least one spiral groove is formed in the outer side of the shaft. The number of the tooth-shaped protrusions in the shaft holes of the sheet parts and the number of the spiral grooves in the outer side of the shaft are the same. The tooth-shaped protrusions are matched with the spiral grooves. The shaft is tightly sleeved with the sheet parts in order, the relative positions between the shaft and the sheet parts are fixed, and the gaps between the shaft and the sheet parts are filled with cured glue. The technical purpose of quickly manufacturing the compressor with high physical performance at a low cost is achieved.

The invention discloses a novel floor. The novel floor comprises a floor body, a floor base arranged below the floor body and fixing components used for connecting the floor body and the floor base, wherein the floor body comprises a floor tread layer and fitting parts arranged on two sides of the floor tread layer in the width direction, as well as first connecting parts, a second connecting part and a reinforcing bar which are sequentially arranged on the lower surface of the floor tread layer in the width direction; and the floor base comprises first buckle parts arranged at the periphery of the floor base, as well as second buckle parts, first slideways and a reinforcing groove which are arranged on the upper surface of the floor base, and vent holes formed in the lower part of the floor base. The novel floor is simple in structure, convenient to mount and dismount, is water-proof, damp-proof and nonhazardous and is suitable for large-scale popularization and application.

The invention discloses Serratia marcescens for degrading sodium lauryl sulfate and a use thereof and belongs to the technical field of environmental microbial application. The Serratia marcescens strain is named as Serratia marcescens GW-1, has a preservation number of CCTCC NO: M2015246, is isolated from the soil rich in SDS, is in a shape of a nearly spherical short rod, has a small volume, is gram-negative and does not produce spores. The colony cultured on a LB plate at 37 DEG C is non-transparent, smooth, moist and white and has the raised center and the irregular edge. The Serratia marcescens cultured on the LB plate at 28 DEG C can produce a red pigment. The Serratia marcescens GW-1 (CCTCC NO: M2015246) realizes effective degradation of sodium dodecyl sulfate and can be used to prepare a preparation for degradation of SDS.

The present invention relates to a silk fibroin microneedle electrode, which comprises a silk fibroin microneedle body and a platinum wire electrode portion, the silk fibroin microneedle body comprises a substrate and a plurality of solid needle bodies uniformly implanted on one side surface of the substrate, the silk fibroin microneedle has high hardness in a dry state but forms a plurality of pores for the passage of glucose after absorbing the liquid, and the components of the silk fibroin microneedle body include silk fibroin, polyhydric alcohol and glucose oxidase; the platinum wire electrode portion extends from the inside of the solid needle body away from the solid needle body, and the platinum wire electrode portion includes a platinum wire body, a catalyst layer and a carrier layer which are sequentially disposed from the inside to the outside; The catalyst layer is a glucose oxidase layer and the carrier layer is a silk fibroin layer. The preparation method has the advantages of simple preparation method, low cost, wide detection range, good biocompatibility and high electrode stability.

The invention relates to a rainwater garden capable of intensifying nitrogen and phosphorus removal. The rainwater garden comprises a foundation pit and a water collecting pool, wherein a foundation pit gravel layer, a foundation pit zeolite layer, a foundation pit planting layer and a foundation pit organic matter layer are sequentially arranged in the foundation pit from bottom to top; a gap isreserved between the top face of the foundation pit organic matter layer and a top pit opening of the foundation pit to form a water storage space; aquatic herbaceous plants or gramineous plants are planted in the foundation pit planting layer; earthworms are inoculated in the foundation pit; a water collecting pool gravel layer, a water collecting pool zeolite layer and a water collecting pool organic matter layer are sequentially arranged in the water collecting pool from bottom to top; the water collecting pool zeolite layer is communicated with the foundation pit gravel layer through a pipeline; the bottom of the water collecting pool gravel layer is communicated with a drainage pipe. Rainwater is collected through the water storage space and subjected to nitrogen and phosphorus removal through plant absorption and nitration reaction in the foundation pit; the rainwater subjected to nitrogen and phosphorus removal is subjected to denitrification reaction in the water collecting pool to enhance nitrogen removal; the removal effect of pollutants such as nitrogen and phosphorus in the rainwater subjected to multi-stage purification treatment is good, nitrogen and phosphorus removal is thorough, and the treated rainwater can be directly recycled.