A method for separating purified seabuckthorn 
flavonoid from large 
berry seabuckthorn marc. The invention relates to a method for separating purified seabuckthorn flvonoid from large 
berry seabuckthorn marc. The invention solves the problems in the existing separation and purification method of seabuckthorn 
flavonoid, such as tedious method, complicated techniques, high cost of separation and purification, easy damage to the activity of 
flavonoid and low purity of flavonoid. The method for separating purified seabuckthorn flavonoid from large 
berry seabuckthorn marc disclosed by the invention comprises the following steps: first, 
degreasing the raw materials; second, ultrasonic wave extracting and centrifugating; third, adding 
metal salt to extracting solution to lead complex reaction between flavonoid and 
metal, agitating, 
tempering the pH of solution and collecting 
sediment; and fourth, adding decomplexing additive to resolve 
sediment, concentrating in a decompressing way to 
dryness, using 
ethanol to dissolve and filter, concentrating filtrate, and obtaining the separated and purified seabuckthorn flavonoid 
powder after 
drying. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention can ensure the full improvement of the purity of the purified seabuckthorn flavonoid, and maintains the original 
biological activity of the natural flavonoid, the seabuckthorn flavonoi is safe and nontoxic, and the method disclosed by the invention has the advantages of low production cost and simple process, and is suitable for the large-scale production of the seabuckthorn flavonoid.