38results about How to "Qualitatively suitable for" patented technology

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content ofthe porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is appl icable in screening mass samples.

The present invention provides an enzyme-linked immunosorbent assay kit for dibutyl phthalate detection. The enzyme-linked immunosorbent assay kit comprises: a coating antigen-coated enzyme label plate, specific dibutyl phthalate antibody, an enzyme marker, a dibutyl phthalate standard substance solution, a substrate coloration solution, a stop buffer, a cleaning solution and a sample diluent, wherein the coating antigen is a conjugate formed by conjugating dibutyl phthalate to antigen, and the enzyme marker is an enzyme-labeled anti-antibody. The invention further discloses a method for applying the enzyme-linked immunosorbent assay kit to detect dibutyl phthalate, wherein the method comprises: carrying out a sample pretreatment, adopting the kit to detect, and analyzing the detection result. The enzyme-linked immunosorbent assay kit can be provided for detect the dibutyl phthalate content in the white spirit, has characteristics of simple operation, low cost and high sensitivity, can be provided for on-site monitoring, and is suitable for screening large number of samples.

The present invention discloses a method for detecting sulfaquinoxaline and its special-purpose ELIA kit. Said ELIA kit includes sulfaquinoxaline specific antibody, coating source and enzyme label. The described coating source is the conjugate of sulfaquinoxaline semiantigen and carrier protein or sulfaquinoxaline antiantibody, the described enzyme label is enzyme labeled sulfaquinoxaline antiantibody or enzyme labeled fulfaquinoxaline semiantigen. When the coating source is conjugate of the sulfaquinoxaline semiantigen and carrier protein, the described enzyme label is enzyme labeled sulfaquinoxaline antiantibody; and when the described coating source is sulfaquinoxaline antiantibody, the described cenzyme label is enzyme-labelled sulfaquinoxaline semiantigen.

The invention provides an enzyme linked immunosorbent assay kit detecting folic acid. The enzyme linked immunosorbent assay kit comprises an elisa plate coated with a coating antigen, a folic acid antibody working solution, an enzyme marker, a folic acid standard substance solution, a substrate developing solution, a stop solution, a scrubbing solution and a compound solution. The coating antigen is a folic acid coupling antigen, and the enzyme marker is an enzyme marking antibody. The invention further discloses a method applying the enzyme linked immunosorbent assay kit detecting the folic acid. The method comprises the steps that sample pretreatment is carried out at first, then the kit is used for detection, and finally the detection result is analyzed. The enzyme linked immunosorbent assay kit can be used for detecting the content of the folic acid in finished milk, milk powder and cereals such as rice, millet, corns, soybeans and flour. The kit is easy and convenient to operate, low in cost, high in sensitivity, capable of monitoring the folic acid on site, and suitable for screening a large number of samples.

The invention discloses an enzyme immunity test agent box for testing the apurone drug, which comprises: an enzyme mark plate that coated by coat source; enzyme mark matter; apurone specific antibody working solution (when the enzyme mark plate is coated by antibody and the enzyme mark matter is the enzyme mark antibody or the enzyme mark plate is coated by the anti antibody and the enzyme mark matter is the enzyme mark antigen); apurone standard solution; base color display solution; stop solution; concentrated washing solution; and concentrated compound solution. The invention also discloses a method for using said agent box to test apurone, which comprises: processing pretreatment on the sample; then using test box to test; at last analyzing the test result. The inventive agent box can be used to test the left amount of apurone in chicken and shrimp, etc, with simple operation, lower cost, and high sensitivity.

The invention provides an enzyme-linked immunosorbent assay kit for detecting Sudan red and paranitroaniline red medicaments. The enzyme-linked immunosorbent assay kit comprises an enzyme-linked immunosorbent assay plate coated with a coating antigen, an enzyme label, a Sudan red specific antibody working solution (occurring when the antigen is coated on the enzyme-linked immunosorbent assay plate and the enzyme label is of an enzyme-labeled anti-antibody or the anti-antibody is coated on the enzyme-linked immunosorbent assay plate and the enzyme label is of an enzyme-labeled antigen), a Sudan red standard product solution, a substrate color development solution, a stop solution, a concentrated cleaning solution and a concentrated complex solution. The invention further discloses a method for detecting Sudan red and paranitroaniline red by applying the enzyme-linked immunosorbent assay kit, and the method comprises the following steps: firstly performing pretreatment on a sample, then detecting through the kit and finally analyzing a detection result. The enzyme-linked immunosorbent assay kit provided by the invention can be used for detecting the content of the Sudan red and the paranitroaniline red in the samples, such as chili sauce, chili oil, feed and the like, has the advantages of simplicity and convenience in operation, low cost, high sensitivity and capability of performing site monitoring, and is suitable for screening a large number of the samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting medroxyprogesterone, which comprises an enzyme label plate coated with a coating antigen, an enzyme marker, a medroxyprogesterone standard substance solution, a substrate coloured solution, a stopping solution, a washing liquid and a reconstitution fluid, the coating antigen is the medroxyprogesterone coupling antigen, and the enzyme marker is an enzymic-labelled antibody. The invention also discloses a method for detecting medroxyprogesterone by using the enzyme linked immunosorbent assay kit, which comprises the following steps: pre-treating a sample, then detecting by the kit, and finally analyzing the detection result. The enzyme linked immunosorbent assay kit can be used for detecting the medroxyprogesterone content in chicken, fish and shrimp samples, operation is simple, cost is low, sensitivity is high, and the kit enables on-site monitor and is suitable for screening a lot of samples.

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for Cimaterol detection. The kit comprises an ELISA plate coated with a coating antigen, a Cimaterol standard substance solution, a Cimaterol antibody, an enzyme conjugate concentrate, an enzyme conjugate diluent, a substrate developing solution, a stop solution and a cleaning solution, wherein the coating antigen is a Cimaterol coupling antigen, an enzyme conjugate is the enzyme-labeled Cimaterol antibody, and the Cimaterol antibody is obtained from an immunogenic immune animal. The invention further discloses a method ofapplying the ELISA kit to Cimaterol detection. The method comprises the steps that first, sample pretreatment is performed; second, the kit is used for detection; and last, a detection result is analyzed. The ELISA kit can be used for detecting the content of Cimaterol in animal tissue and swine urine samples, is easy and convenient to operate, low in cost and high in sensitivity, can be monitored on spot and is suitable for screening of a large quantity of samples.

The invention provides a preparation method of an antibody for bovine serum albumin and other impurities in a vaccine, and relates to the following steps: directly immunizing animals to obtain a bovine serum albumin antibody, preparing neomycin immunogen from an impurity neomycin antibody, immunizing animals with the neomycin immunogen, and applying the obtained antibody to an enzyme-linked immunosorbent assay kit.

The invention provides an enzyme linked immunosorbent assay kit for detecting fenpropathrin. The enzyme linked immunosorbent assay kit comprises an ELISA plate coated with a coating antigen, a fenpropathrin standard substance solution, a fenpropathrin antibody, an enzyme conjugate concentrated solution, an enzyme conjugate diluent, a substrate developing solution, a terminating solution and a washing solution, wherein the coating antigen is a fenpropathrin coupled antigen, the enzyme conjugate is an enzyme-labeled fenpropathrin antibody, and the fenpropathrin antibody is obtained by immunizinganimals with immunogen. The invention further discloses a method for detecting fenpropathrin by applying the enzyme linked immunosorbent assay kit. The method comprises the following steps of: pretreating a sample, detecting the sample by using the kit, and finally analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of fenpropathrin in vegetable and fruit samples, is simple and convenient to operate, low in cost and high in sensitivity, can be monitored on site, and is suitable for screening a large number ofsamples.

The invention provides an enzyme-linked immunoassay kit for detecting a fluoroquinolone medicine. The enzyme-linked immunoassay kit comprises an enzyme-labelled plate coated with a coating antigen, a standard solution of the fluoroquinolone medicine, an enzyme-labelled bivalent antibody, a specific antibody of the fluoroquinolone medicine, a developing solution of a substrate, a stop solution, a washing solution, a combination solution and a diluent of a honey sample; the coating antigen is a coupled antigen of the fluoroquinolone medicine; and the enzyme-labelled bivalent antibody is an enzyme-labelled anti-goat and anti-rat antibody. The invention further discloses a method for detecting the fluoroquinolone medicine by using the enzyme-linked immunoassay kit. The method comprises the following steps: pre-processing a sample at first, then, detecting by using the kit, and finally, analyzing a detection result. The enzyme-linked immunoassay kit provided by the invention can be used for detecting the content of the fluoroquinolone medicine in honey, chicken, pork, fish, shrimp, milk, milk powder and egg samples, is simple and convenient to operate, low in cost and high in sensitivity and can be used for monitoring on site and applied to screening of a lot of samples.

The invention provides a carbendazim detection ELISA (enzyme linked immunosorbent assay) kit which includes an ELISA plate coated with a coating antigen, a carbendazim standard solution, a carbendazim antibody, an enzyme-labeled secondary antibody, a substrate coloring liquid, a terminating liquid, a washing liquid and a complex solution, the coating antigen is a carbendazim coupling antigen, and the enzyme-labeled secondary antibody is an enzyme labeled carbendazim antibody. The invention also discloses a carbendazim detection method using the ELISA kit, and the method includes first, pretreatment of a sample, then detection using the ELISA kit and final detection result analyzing. The ELISA kit can be used to detect the content of carbendazim in a tobacco leaf sample, and has the advantages of being simple in operation, low in cost, high in sensitivity, capable of monitoring on site and suitable for screening a large number of samples.

The invention provides an enzyme-linked immunosorbent assay kit for detecting ivermectin and abamectin. The enzyme-linked immunosorbent assay kit comprises an ELISA plate coated with an ivermectin coupling antigen, an ivermectin monoclonal antibody, an enzyme-labeled antibody, an ivermectin standard substance solution, a solution substrate colored solution, a stopping solution, a washing solutionand a combination solution. The invention also discloses a method for detecting the residual quantity of ivermectin and abamectin by using the enzyme-linked immunosorbent assay kit. The method comprises the following steps: firstly performing pretreatment on a sample, then detecting the sample by using a kit, and finally analyzing the detection result. The enzyme-linked immunosorbent assay kit provided by the invention can be used for detecting the residual quantities of ivermectin and abamectin in animal tissues, milk and water. The enzyme-linked immunosorbent assay kit provided by the invention is simple and convenient in operation, low in cost and high in sensitivity, which can be used for the field monitoring and the screening of a large number of samples.

The present invention discloses a method for detecting 19-nandrolone and its special-purpose ELIA kit. Said kit includes 19-nandrolone specific antibody, coating source and enzyme label. The described coating source is conjugate of 19-nandrolone semiantigen and carrier protein or antiantibody. The described enzyme label is enzyme-labelled antiantibody or enzyme-labelled 19-nandrolone semiantigen. When the described coating source in the conjugate of 19-nandrolone semiantigen and carrier protein, the described enzyme label is enzyme-labelled antiantibody, and when the described coating source is anti-antibody, the described enzyme label is the enzyme-labelled 19-nandrolone semi-antigen.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content of the porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is applicable in screening mass samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting ribavirin. The kit comprises an elisa plate coated with a coating antigen, a ribavirin standard substance solution, an enzyme-labeled antibody, substrate developing liquid, a stop solution, a scrubbing solution and reconstitution fluid. The coating antigen is a ribavirin coupled antigen, and the enzyme-labeled antibody is an enzyme-labeled ribavirin antibody. The invention further discloses a method for detecting ribavirin through the enzyme linked immunosorbent assay kit. The method comprises the steps that firstly, sample pretreatment is conducted; then, detection is conducted through the kit; finally, a detection result is analyzed. The enzyme linked immunosorbent assay kit can be used for detecting the content of ribavirin in animal tissue samples, and is easy and convenient to operate, low in expenditure, high in sensitivity and suitable for screening a large number of samples, and on-site monitoring is achieved.

The invention discloses an ELISA kit for detecting safrole, which comprises: an enzyme label plate coated with a coating source, a safrole standard solution, a safrole specific antibody, an enzyme-labeled secondary antibody, a substrate chromogenic solution, a termination solution, washing solution, and reconstitution solution; the original coating is safrole-conjugated antigen, the enzyme-labeled secondary antibody is enzyme-labeled goat anti-mouse anti-antibody, and the safrole-specific antibody is prepared by using safrole-conjugated antigen as an immunogen; the safrole The coupled antigen is obtained by coupling a safrole hapten with a carrier protein, and the safrole hapten is composed of 5‑(2‑bromo‑2‑propene‑1‑yl)‑1,3‑benzodioxolane and amino Propionic acid reaction to get. The invention also discloses a method for detecting safrole using the enzyme-linked immunosorbent assay kit. The ELISA kit provided by the invention can be used to detect the content of safrole in samples, and has the advantages of simple operation, low cost, high sensitivity, on-site monitoring and suitable for screening of a large number of samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting a trimethoprim medicament and application thereof. The enzyme linked immunosorbent assay kit comprises an enzyme label plate coated with envelope antigen, an enzyme marker, trimethoprim specificity antibody working solution (when the enzyme label plate is coated with the envelope antigen and the enzyme marker is enzyme-labeled anti-antibody or the enzyme label plate is coated with the envelop antigen and the enzyme marker is the enzyme-labeled antigen), trimethoprim standard solution, substrate developing solution, stop solution, concentrated washing solution and composite solution. The invention also discloses a method for detecting the trimethoprim by applying the enzyme linked immunosorbent assay kit. The method comprises the following steps of: performing sample pretreatment; detecting by the kit; and analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the residual amount of the trimethoprim in a honey sample, has the advantages of simple operation, low expense and high sensitivity, can be monitored in field, and is suitable for screening mass samples.

The invention provides an ELISA kit for detecting E3. The ELISA kit for detecting E3 comprises an enzyme label plate coated with a coating antigen, an E3 standardized product solution, an enzyme labeled secondary antibody, a substrate chromogenic solution, a stopping solution, washing liquid and a compound solution, the coating antigen is an E3 conjugated antigen, and the enzyme labeled secondary antibody is an enzyme labeled E3 secondary antibody. The invention further discloses a method for detecting E3 by using the ELISA kit, comprising the following steps: firstly, carrying out pretreatment on a sample and then detecting by using the kit, finally, analyzing detection results. The ELISA kit provided by the invention can be used for detecting the content of E3 in animal derived food and water samples, and is simple and convenient to operate, low in cost, high in sensitivity and capable of carrying out on-site monitoring and suitable for screening a large number of the samples.

The invention provides an enzyme-linked immunosorbent assay kit for detecting Sudan red and paranitroaniline red medicaments. The enzyme-linked immunosorbent assay kit comprises an enzyme-linked immunosorbent assay plate coated with a coating antigen, an enzyme label, a Sudan red specific antibody working solution (occurring when the antigen is coated on the enzyme-linked immunosorbent assay plate and the enzyme label is of an enzyme-labeled anti-antibody or the anti-antibody is coated on the enzyme-linked immunosorbent assay plate and the enzyme label is of an enzyme-labeled antigen), a Sudan red standard product solution, a substrate color development solution, a stop solution, a concentrated cleaning solution and a concentrated complex solution. The invention further discloses a method for detecting Sudan red and paranitroaniline red by applying the enzyme-linked immunosorbent assay kit, and the method comprises the following steps: firstly performing pretreatment on a sample, then detecting through the kit and finally analyzing a detection result. The enzyme-linked immunosorbent assay kit provided by the invention can be used for detecting the content of the Sudan red and the paranitroaniline red in the samples, such as chili sauce, chili oil, feed and the like, has the advantages of simplicity and convenience in operation, low cost, high sensitivity and capability of performing site monitoring, and is suitable for screening a large number of the samples.

The invention provides an enzyme-linked immunosorbent assay kit for detecting quinclorac. The enzyme-linked immunosorbent assay kit comprises an ELISA plate coated with a coating antigen, a quinclorac standard substance solution, a quinclorac antibody, an enzyme-labeled second antibody, a substrate developing solution, a stop solution, a scrubbing solution and reconstitution fluid, wherein the coating antigen is a quinclorac coupling antigen, and the enzyme-labeled second antibody is an enzyme-labeled quinclorac antiantibody. The invention further discloses a method for detecting quinclorac with the enzyme-linked immunosorbent assay kit. The method includes the steps that firstly, sample pretreatment is carried out; then, detection is carried out with the kit; finally, a detection result is analyzed. The enzyme-linked immunosorbent assay kit can be used for detecting the content of quinclorac in a soil sample and is easy and convenient to operate, low in cost, high in sensitivity, capable of being monitored on site and suitable for screening of a large number of samples.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting amantadine. The ELISA kit comprises an ELISA plate coated with a coating antigen, an amantadine standard product solution, an enzyme-labeled antibody, a substrate developing solution, a stop solution, a washing solution and a combination solution, wherein the coating antigen is an amantadine coupling antigen, and the enzyme-labeled antibody is an enzyme-labeled amantadine antibody. The invention further discloses a method for applying the ELISA kit to amantadine detection. The method comprises steps as follows: sample pretreatment is performed firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of amantadine in an animal tissue sample, is simple and convenient to operate, low in cost and high in sensitivity, can be used for performing on-site monitoring and is suitable for screening a large number of samples.

The invention provides an enzyme-linked immunosorbent assay kit for detecting protein A and other impurities in a biological product, which comprises an elisa plate coated with a coating antigen, a protein A standard substance solution, a gentamicin standard substance solution, a protein A antibody, a gentamicin antibody, an enzyme conjugate concentrated solution, an enzyme conjugate diluent, a substrate developing solution, a stop solution and a washing solution; the coating antigen is a gentamicin coupling antigen, the enzyme conjugate is an enzyme-labeled protein A antibody and an enzyme-labeled gentamicin antibody, and the protein A antibody and the gentamicin antibody are both obtained by immunizing an animal with an immunogen. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of impurities such as protein A in biological products, is simple and convenient to operate, low in cost and high in sensitivity, can be used for on-site monitoring and is suitable for screening a large number of samples.

The invention provides an ELISA kit for detecting coumarin and its application. The kit includes: ELISA plate coated with the original coating, coumarin standard solution, enzyme-labeled secondary antibody, coumarin-specific antibody, substrate chromogenic solution, stop solution, washing solution, reconstitution solution; The coating is originally a coumarin-coupled antigen, the enzyme-labeled secondary antibody is an enzyme-labeled goat anti-mouse anti-antibody, and the coumarin-specific antibody is prepared by using a coumarin-coupled antigen as an immunogen ; The coumarin-coupled antigen is obtained by coupling a coumarin hapten with a carrier protein, and the coumarin hapten is obtained by acetalization of coumarin. The enzyme-linked immunosorbent assay kit provided by the invention can be used to detect the content of coumarin in tobacco and tobacco flavors and fragrances, and has the advantages of simple operation, low cost, high sensitivity, on-site monitoring and suitable for screening of a large number of samples.

The invention provides an enzyme-linked immunoassay kit for detecting coumarin and an application thereof. The kit comprises an enzyme-labeled plate coated with a coating antigen, a coumarin standardsolution, an enzyme-labeled secondary antibody, a coumarin-specific antibody, a substrate color developing solution, a stop solution, a washing solution and a complex solution. The coating antigen isa coumarin-coupled antigen. The enzyme-labeled secondary antibody is an enzyme-labeled goat anti-mouse anti-antibody. The coumarin-specific antibody is prepared through a coumarin-conjugated antigen as an immunogen. The coumarin-conjugated antigen is obtained through coupling a coumarin hapten to a carrier protein. The coumarin hapten is obtained through acetalization of coumarin. The enzyme-linked immunoassay kit can be used for detecting the content of coumarin in tobacco and tobacco flavors and fragrances, is easy to operate, has a low cost and high sensitivity and realizes on-site monitoring and screening of a large amount of samples.

The present invention provides an enzyme-linked immunosorbent assay kit for detecting morphine and codeine. The enzyme-linked immunosorbent assay kit comprises an enzyme label plate coated with a morphine-conjugated antigen, a morphine monoclonal antibody, an enzyme-labeled anti-antibody, a morphine standard substance solution, a substrate coloring solution, a stopping solution, a washing liquid and a reconstituted solution. The invention further discloses a method for using the enzyme-linked immunosorbent assay kit to detect morphine and codeine, wherein the method comprises: pre-treating a sample, detecting with the kit, and analyzing the detection result. According to the present invention, the enzyme-linked immunosorbent assay kit can be used for detecting the residual morphine and the residual codeine in the hot pot bottom material and the chilli sauce, has characteristics of simple operation, low cost, high sensitivity and on-site monitoring, and is suitable for screening of a large number of samples.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight inthe animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention provides an enzyme-linked immunosorbent assay kit for detecting triazophos. The enzyme-linked immunosorbent assay kit comprises an ELISA plate containing a coating antigen, a triazophos standard solution, an ELIAS secondary antibody, a triazophos specificity antibody, a substrate color development solution, a stopping solution, a cleaning solution and a re-dissolving solution, wherein the coating antigen is a triazophos coupling antigen; the ELIAS secondary antibody is an enzyme-labeled goat-anti-mouse IgG antibody. The invention further discloses a method for applying the enzyme-linked immunosorbent assay kit to detection of triazophos. The method comprises the following steps: first, carrying out sample pretreatment; then, carrying out the detection with the enzyme-linked immunosorbent assay kit; finally, analyzing the detection result. The enzyme-linked immunosorbent assay kit provided by the invention can be utilized for detecting the content of triazophos in a vegetable sample, has an on-site monitoring function, and is simple and convenient to operate, low in cost, high in sensitivity, and suitable for screening of lots of samples.