The invention belongs to the technical field of bacterium cultivation, and discloses a bag cultivation method for inserting a tube into the middle of a shiitake 
mushroom short bag for inoculating ripematerials. A bag body and a hollow tube are arranged; the bag body is filled with culture materials, a circular hole is formed in the middle of the culture materials, one end of the hollow tube is filled with cotton, the hollow tube is inserted into the circular hole of the bag body, and the end filled with the cotton faces upwards. Accordingly, by 
punching the middle of the material bag and inserting the tube, in the 
sterilization process, 
high pressure steam penetrates through the material bag conveniently, the disinfection and sterilization time is short, sterilization is conducted thoroughly easily, the 
infection rate is lowered, 
bacteria inoculated in the middle make contact with the center of the bag materials, heat preservation and 
moisture retention are achieved, the cotton is good in air permeability, growth 
oxygen supply is facilitated, the 
bacteria germinate rapidly, spawn runing is good, and spread and growth in the materials are rapid; the sterilized cotton blocks miscellaneous 
bacteria invasive infection, and culture period 
pollution is reduced. For intermediate 
inoculation, compared with single end short bag 
inoculation and long bag 
peripheral punching or nail 
inoculation, the 
hypha bagfull time is shortened, long bag overgrowing time needs 60-90 d, and overgrowing of short bag intermediate inoculation only needs 25-30 d.