Carbon dioxide diagnosis/determination reagent kit and method for determining carbon dioxide concentration

[0107] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0108] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0109] Stabilizer 500mmol / L

[0110] Reduced coenzyme 0.25mmol / L

[0111] Acetic acid 20mmol / L

[0112] Ferrocytochrome b1 5mmol / L

[0113] Ammonia ion 50mmol / L

[0114] Pyruvate oxidase 10000U / L

[0115] Alanine dehydrogenase 10000U / L

[0116] Glycine oxidase 10000U / L

[0117] Peroxidase 30000U / L

[0118] 4-amino antiarsenic 2mmol / L

[0119] Carbolic acid 10mmol / L

[0120] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0121] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, and t...

[0124] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0125] Reagent 1

[0126] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0127] Stabilizer 50mmol / L

[0128] Reduced coenzyme 0.25mmol / L

[0129] Acetic acid 20mmol / L

[0130] Ferrocytochrome b1 5mmol / L

[0131] Ammonia ion 50mmol / L

[0132] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0133] Reagent 2

[0134] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0135] Stabilizer 500mmol / L

[0136] Pyruvate oxidase 10000U / L

[0137] Alanine dehydrogenase 10000U / L

[0138] Glycine oxidase 10000U / L

[0139] Peroxidase 30000U / L

[0140] 4-amino antiarsenic 0.6mmol / L

[0141] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0142] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, ...

[0145] The carbon dioxide diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0146] Reagent 1

[0147] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0148] Stabilizer 50mmol / L

[0149] Reduced coenzyme 0.25mmol / L

[0150] Acetic acid 20mmol / L

[0151] Ferrocytochrome b1 5mmol / L

[0152] Ammonia ion 50mmol / L

[0153] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0154] Reagent 2

[0155] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0156] Stabilizer 500mmol / L

[0157] Alanine dehydrogenase 10000U / L

[0158] Glycine oxidase 10000U / L

[0159] Peroxidase 30000U / L

[0160] Dimethylaniline 2mmol / L

[0161] Reagent 3

[0162] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0163] Stabilizer 500mmol / L

[0164] Pyruvate oxidase 10000U / L

[0165] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which c...