Anti-stress feed additive based on purification chelation process, and preparation method and application thereof

[0041] An animal anti-stress feed additive, said additive comprises: chitosan oligosaccharide potassium, glucose oxidase, silicon dioxide, and the composition content of its additive is:

[0042] Potassium chitosan oligosaccharide 60 parts

[0043] Glucose oxidase 15 parts

[0044] Silica 5 parts

[0045]The potassium chitosan is obtained by an enzyme reaction; the specific steps of the enzyme reaction are: adding cellulase and glucose oxidase ( Add cellulase and glucose oxidase at one time, the mass ratio of the total amount of cellulase added to chitosan is 1:150, the mass ratio of the total amount of glucose oxidase added to chitosan oligosaccharide is 1:150), flow Add 15% dipotassium hydrogen phosphate solution (the solvent is water), the flow rate is 2 liters / min, and the feeding time is 2 hours to maintain the pH of the solution at 5.0-7.0 and maintain the temperature at 55±0.5°C. The ventilation volume is 1vvm, the tank pressure is maintained at 0.2-0.5MPa, the stirr...

[0048] An animal anti-stress feed additive, said additive comprises: chitosan oligosaccharide potassium, glucose oxidase, silicon dioxide, and the composition content of its additive is:

[0049] Potassium chitosan 80 parts

[0050] Glucose oxidase 15 parts

[0051] Silica 5 parts

[0052] The preparation steps of this example are the same as those of Example 1, and will not be described in detail here.

[0054] An animal anti-stress feed additive, the additive includes: chitosan oligosaccharide potassium, glucose oxidase, silicon dioxide, and the composition content of the additive is:

[0055] Potassium chitosan oligosaccharide 52 parts

[0056] Glucose oxidase 20 parts

[0057] Silicon dioxide 8 parts

[0058] The preparation steps of this example are the same as those of Example 1, and will not be described in detail here.