274 results about "Ventilation volume" patented technology

The apparatus provides for the determination of the instantaneous phase in the respiratory cycle, subject's average respiration rate and the provision of ventilatory assistance. A microprocessor (16) receives an airflow signal from a pressure transducer (18) coupled to a port (17) at a mask (11). The microprocessor (16) controls a servo (19), that in turn controls the fan motor (20) and thus the pressure of air delivered by the blower (10). The blower (10) is coupled to a subject's mask (ii) by a conduit (12). The invention seeks to address the following goals: while the subject is awake and making substantial efforts the delivered assistance should be closely matched in phase with the subject's efforts; the machine should automatically adjust the degree of assistance to maintain at least a specified minimum ventilation without relying on the integrity of the subject's chemoreflexes; and it should continue to work correctly in the presence of large leaks.

Method and apparatus for the treatment of cardiac failure, Cheyne Stokes breathing or central sleep apnea are disclosed. A subject is provided with ventilatory support, for example positive pressure ventilatory support using a blower and mask. Respiratory airflow is determined. From the respiratory airflow are derived a measure of instantaneous ventilation (for example half the absolute value of the respiratory airflow) and a measure of longterm average ventilation (for example the instantaneous ventilation low pass filtered with a 100 second time constant). A target ventilation is taken as 95% of the longterm average ventilation. The instantaneous ventilation is fed as the input signal to a clipped integral controller, with the target ventilation as the reference signal. The output of the controller determines the degree of ventilatory support. Clipping is typically to between half and double the degree of support that would do all the respiratory work. A third measure of ventilation, for example instantaneous ventilation low pass filtered with a time constant of 5 seconds, is calculated. Ventilatory support is in phase with the subject's respiratory airflow to the fuzzy extent that this ventilation is above target, and at a preset rate conversely.

Method and apparatus for the treatment of cardiac failure, Cheyne Stokes breathing or central sleep apnea are disclosed. A subject is provided with ventilatory support, for example positive pressure ventilatory support using a blower and mask. Respiratory airflow is determined. From the respiratory airflow are derived a measure of instantaneous ventilation (for example half the absolute value of the respiratory airflow) and a measure of longterm average ventilation (for example the instantaneous ventilation low pass filtered with a 100 second time constant). A target ventilation is taken as 95% of the longterm average ventilation. The instantaneous ventilation is fed as the input signal to a clipped integral controller, with the target ventilation as the reference signal. The output of the controller determines the degree of ventilatory support. Clipping is typically to between half and double the degree of support that would do all the respirator work. A third measure of ventilation, for example instantaneous ventilation low pass filtered with a time constant of 5 seconds, is calculated. Ventilatory support is in phase with the subject's respiratory airflow to the fuzzy extent that this ventilation is above target, and at a preset rate conversely.

A method provides a ventilation control compliant with an adopted ventilation standard for efficient energy usage and conservation of energy resources. The method includes operating a home energy system to generate solar energy within a daily active period and draw fresh air from an ambient region, setting a daily ventilation period as a fractional period of a day, the ventilation period being coincident with the active period during a heating period for the home or a time period after the active period during a cooling period for the home, determining a target volume, determining a flow rate for delivering the fresh air during the ventilation period, performing ventilation in the ventilation period to deliver the fresh air into the home using the flow rate, and monitoring an accumulated total ventilation volume of the delivered fresh air until the accumulated total ventilation volume is within a vicinity of a target volume.

A ventilator supplies a plurality of volumes of fluid to a patient during a like plurality of inhalations by the patient. Each volume of fluid is supplied at an inspiratory positive airway pressure during a corresponding inhalation by the patient. A volume of fluid received by the patient is determined for each of the plurality of inhalations by the patient and an average volume of fluid received by the patient during each of the plurality of inhalations is determined. The average volume of fluid received by the patient during each inhalation is compared to a predetermined target volume and the inspiratory positive airway pressure each volume of fluid is supplied to the patient is adjusted as a function of the comparison.

A servoventilator control slowly changes the target ventilation over a period of time, according to a preprogrammed schedule adapted to be set by the physician. Preferably, the target ventilation stays constant at an initial target ventilation for an initial hold time, and then increases at a constant rate until it reaches a final target ventilation, whereupon it stays constant thereafter. If the pressure support level is too high, possibly indicating glottic or upper airway closure, the rate of increase of target ventilation may be lowered or the final target ventilation not reached.

The invention discloses a microbubble generation device and a use thereof. The microbubble generation device comprises an input pipe, a Venturi tube and an output pipe, and the three parts are orderly communicated. The input pipe, the Venturi tube and the output pipe are arranged on the same straight line. The Venturi tube comprises a contraction section, a throat section and an expansion section, and the three parts of orderly communicated. The outer pipe wall of the throat section is provided with a ventilation ring. The ventilation ring is a hollow columnar sealed chamber. The columnar body of the hollow columnar sealed chamber is provided with a gas inlet. The pipe wall of the throat section is provided with thorough vents symmetrically distributed along the axle center of the Venturi tube. The cross section formed from the vents is perpendicular to the axis of the Venturi tube. The vents are located in the hollow columnar sealed chamber. The device can uniformly feed gas into a liquid so that micro-bubbles are produced in the liquid. The ventilatory capacity and the number and sizes of the bubbles can be adjusted and effectively controlled by device structure adjustment.

The invention provides an oxygen-air hybrid mechanism which comprises shells (3, 4) and a mandrel (1), wherein the shells is provided with a hybrid cavity, a first cavity and a second cavity which are respectively arranged at both sides of the hybrid cavity, and an exhaust port (13) of the first cavity is communicated with an exhaust port (14) of the second cavity by the hybrid cavity; the mandrel (1) can axially move and pass through the first cavity, the hybrid cavity and the second cavity in sequence; the mandrel (1) is provided with a step part (15) which is accommodated in the hybrid cavity; and the axial direction along which the step part (15) moves can simultaneously change the opening of the two exhaust ports (13,14). The invention also provides a breathing machine which comprises the oxygen air hybrid mechanism and a flow proportional valve which is communicated with a quick connection adapter (6) of the oxygen-air hybrid mechanism so as to control the transmission gas flow. The invention can realize the precise control of the oxygen concentration in the condition of less ventilation volume and larger flow and can work in the higher pressure condition.

The region extraction unit extracts lung regions from three-dimensional images of a plurality of time phases, the alignment unit aligns pixel position in the lung region extracted from each three-dimensional image between the three-dimensional images. This calculates a displacement vector field at each time phase of the three-dimensional images. The function generation unit calculates a local ventilation volume function representing a temporal change in ventilation volume at each point in the displacement vector field, and the quantification unit calculates a difference function, which is a function of difference values between the local ventilation volume function and benchmark ventilation volume function, as a quantitative value representing a difference between the local ventilation volume function and the benchmark ventilation volume function. The display control unit displays lung VR images on which the difference function is mapped on the display in time series order.

A method to maintain isocapnia for a subject. A fresh gas is provided to the subject when the subject breathes at a rate less than or equal to the fresh gas flowing to the subject. The fresh gas flow equal to a baseline minute ventilation minus a dead space gas ventilation of the subject contains a physiological insignificant amount of CO2. An additional reserve gas is provided to the subject when the subject breathes at a rate more than the fresh gas flowing to the subject. The reserve gas has a partial pressure of carbon dioxide equal to an arterial partial pressure of carbon dioxide of the subject. A breathing circuit is applied to the method to maintain isocapnia for a subject. The breathing circuit has an exit port, a non-rebreathing valve, a source of fresh gas, a fresh gas reservoir and a reserve gas supply.

The invention relates to a fermenting method for producing pullulanuse polysaccharide, which is characterized in that the method comprises following steps: firstly, preparing seed culture medium, secondly, preparing fermenting initial culture medium, thirdly, inoculating liquid seeds into the fermenting initial culture medium, wherein the inoculation amount is 3-8%, the fermentation stirring speed is 200-500rpm, the fermentation temperature is 29 DEG C +- 1 DEG C, the ventilation volume is 0.5-10V / V, and the bed pressure is 0.01-0.02MPa, fourthly, complementarily adding the other carbon source after fermenting for 24 hours, wherein the adding amount is 40-80g / L and the adding liuqid is sucrose solution or glucose solution or starch hydrolysate whose pH value is 40-60, and continuingly fermenting for 60-72 hours after adding the carbon source, fifthly, flocculating, hyper-filtrating, barrier-separating, concentrating and drying the fermenting liquid and obtaining the pullulanuse polysaccharide whose molecular weight is between 20-60 million daltons. The invention has simple technique and significant effects and prominently shortens the fermenting time, the fermenting time is 80-100 hours, the production efficiency is increased greatly, energy is saved, the yield rate of product is greatly improved, and the yield can reach by 50%.

The invention relates to a multifunctional composite froth suppressing and recovering device used in biological fermentation. The invention is characterized in that the device comprises a novel foam suppressor, an exhaust internal drainage gas tray, a tank gas-liquid separation recoverer, a double L pipe and a recovered gas-liquid dispersing station, wherein, the devices are supported and communicated with one another in application, comprehensively solve three-high problems generally existed in the process of biological fermentation, namely the 'high froth' which affects yield, the 'high ventilation volume' which affects environment and the 'high energy consumption' which affects efficiency. When the device is matched with a bioreactor to use in fermenting production, overflow and liquid escape of a froth layer can be effectively prevented, and liquid loading volume and tank laying yield of fermentation liquid can be improved; meanwhile, environmental pollution is prevented, and obvious effects of energy economization and emission reduction are performed.

The invention discloses a conditioner for remediating organic contaminated soil by utilizing microbes. The conditioner comprises the following components in percentage by mass: 5-20% of microbial flora fermentation liquor, 20-50% of an organic improver and 30-75% of a culture medium, wherein the microbial flora relates to 19 bacterial genera comprising acinetobacter, micrococcaceae, bacillus, candida, trichoderma and aspergillus; the organic improver is one or several of diatomite, humus, straws, charcoal, saw dust, bagasse and plant ash; the culture medium contains monopotassium phosphate, dipotassium phosphate, ammonium sulfate, limestone and bean pulp. The conditioner disclosed by the invention contributes to degrading growth and reproduction of the microbial flora of organic pollutants, and can achieve effects of regulating the pH value of the soil and nutrition-allocated proportion of carbon, nitrogen and phosphorus, effectively improving the composition and structure of the organic contaminated soil and avoiding the phenomenon that the ventilation volume of soil is reduced due to hardening, so that the organic contaminated soil is effectively remediated.

The invention discloses a reaction device for producing organic acid and alcohol through synthesis gas fermentation, comprising a main body reactor and an external gas cycle system which is connected with the main body reactor and cycles the gas generated in the main body reactor, wherein the main body reactor comprises a reactor pot body and a guide cylinder which is arranged in the reactor pot body, is fixed by a support and is a concentric cylinder of the reactor pot body; a blade type blender is arranged along the axis of the guide cylinder, the end, with a blending blade, of the blender is arranged close to the bottom end of the guide cylinder and the other end of the blender is connected with a drive motor; a vent pipe of a gas distributor stretches into the position between the bottom in the guide cylinder and the lower part of the blending blade, and the other end of the gas distributor is communicated with a gas input pipeline by stretching out of the guide cylinder. A cycle gas circuit and a normal gas supply gas circuit are alternately used in the external gas cycle system. The reaction device of the invention has the advantage of simple structure and operation, can obtain good gas-liquid mass transfer without high blending power and ventilation volume, can not only realize continuous gas supply but also realize gas cycle utilization, and greatly improves the use ratio of the gas substrate.

The invention relates to the technical field of road tunnel ventilation, in particular to an environment prediction-based tunnel ventilation control method and system. The tunnel ventilation control method comprises the steps of setting target values of indexes related to a tunnel environment; collecting detection values of the indexes at a current moment; according to the detection values of theindexes and a prediction model built through deep learning, predicting prediction values of the indexes at a next moment; and according to the detection values, the prediction values and the target values of the indexes, adjusting ventilation volume of a tunnel. The tunnel ventilation control system comprises a fan set, a detection apparatus and a control apparatus, wherein the fan set is arrangedin the tunnel; the detection apparatus is arranged corresponding to the fan set; and the control apparatus is connected with the fan set and the detection apparatus. According to the environment prediction-based tunnel ventilation control method and system, the hysteresis of air volume feedback control can be reduced and the ventilation volume control accuracy can be improved.

The invention discloses a carbon tetrachloride separation system and process. The separation system comprises a bottom part containing an S-shaped biochemical tank and a top part with a spraying nozzle, wherein a porous aeration head and a steam nozzle are arranged inside the S-shaped biochemical tank; the porous aeration head is arranged on an air pipe, the steam nozzle is arranged on a steam pipe, the spraying nozzle is arranged on a spraying pipe, and the spraying pipe is used for inputting liquid to be separated. According to the separation process, the ventilation volume is controlled to be 80L / min, the water level is 80cm, the temperature is 60 DEG C and the aeration time is 2h. The carbon tetrachloride separation system and the process have the characteristics of high environmental protection, high resource utilization rate, high processing effect, strong impact resistance and full-automatic control.

The present invention discloses an indirect monitoring method and a monitoring device for grate plate temperature field in grate-rotary kiln-annular cooler palletizing production technique belonging to the technical field of metallurgical industry measuring control. The present invention utilizes a thermocouple temperature measuring sensor to indirectly detect temperature fields in four sections of the grate plate and adjusts ventilation volume of an air draft / extract box in sequence in real time to realize indirect control over grate plate temperature of the grate. The present invention is characterized in that indirect grate plate temperature field detection and control technology is adopted to make the device operate stably and reliably, thus overcoming grate plate temperature fluctuation and improving palletizing quality. Control arithmetic software programs are designed for signal collection, temperature field calculation and dead band and scrambling feed-forward PID to realize normal operation of palletizing grate at the optimal temperature and increase thermal fatigue life of key grate parts by 2 to 3 years. Human-machine interface design based on configuration software technology is characterized in visual image and simple operation.

The elastance and a resistance of a subject being ventilated are determined. The determination of elastance and resistance of the breathing of the subject is made without adjusting the ventilation of the subject to facilitate the determination. That is, the determination of elastance and resistance of the subject is made without manipulating one or more parameters of the ventilation in a manner not dictated by a treatment algorithm that is designed to ventilate the subject effectively and / or comfortably.

The invention relates to a pig-manure compost preparation method with fast temperature rise and dehydration and less composting time. The prior pig-manure high-temperature composting process has the problems of slow temperature rise, high moisture content of compost products, long composting time and the like. The method comprises the steps: mixing pig manure which is produced by large-scale piggeries and has the moisture content of between 70 and 75 percent with fillers, thoroughly decomposed pig-manure compost and bamboo charcoal particles; placing the mixture in a composting groove; composting the mixture by adopting a static forced ventilation mode with the ventilation volume between 20 and 30 m / (h.t) for 30 to 36 days to thoroughly decomposing the compost; and obtaining a pig-manure compost product, wherein the fillers are sawdust or straw. The method can reduce nutrient loss in a composting process and guarantee the fertilizer efficiency of the compost. The method has the advantages of convenient application, low cost, no secondary pollution caused by a production process, significant economic and environmental benefit.

The invention relates to a method for microwave drying of sodium chloride, which comprises the following steps: using the sodium chloride of which the water content is between 0.8 and 4 weight percent as a raw material; adopting a continuous microwave drying device; according to relatively fixed microwave power, changing the ventilation volumes of different microwave cavities, further adjusting parameters such as the transporting rate of materials in the microwave cavities, the thickness of the material and the like; and mounting a stirrer and cavity clapboards in the microwave drying device. The ventilation volumes of ventilation devices in the microwave cavities are controlled to be between 1,247 and 2,250 m<3> / h, the speed rate of a conveying belt is between 0.5 and 2 meters per minute, the thickness of material layers is controlled to be between 1 and 5 centimeters, the stirrer and the cavity clapboards are arranged on the microwave drying device, a roll-type breaking plant is arranged in front of a discharge port, and the particle size of the sodium chloride is controlled to be less than 3 millimeters, thus the method achieves the effect that the mass water content of high-titanium slag is dried to be less than 0.5 percent from 0.8 to 4 percent at drying temperature of between 65 and 70 DEG C.

The invention relates to a non-powered active temperature-adjustment system and a temperature adjusting method. The system comprises a gas source, a regularly confined space, a non-power air-pumping device, a fist connecting device and a second connecting device. When in use, the regularly confined space has high separation rate with external environment; the non-power air-pumping device makes use of natural wind of the external environment and / or internal and external temperature difference of the regularly confined space to form a low atmospheric pressure at a suction opening to pump out the gas at the suction opening, and the gas flow at the gas source is continuously pumped out by the regularly confined space so as to form a heat-exchanging gas flow in the regularly confined space. The invention has the advantages of very simple structure, low investment, no maintaining cost, long service life, energy saving and environmental protection. And according to actual demands, a controlling unit and an auxiliary fan can be added to realize precise control of parameters of temperature, humidity, ventilation volume, processing time and the like of the heat-exchanging gas flow, thereby meeting the actual demands of users.

A method for providing a ventilation control compliant with an adopted ventilation standard for efficient energy usage and conservation of energy resources. The method includes operating a home energy system to generate solar energy within a daily active period and draw ambient fresh air, and setting a daily ventilation period as a fractional period of a day. The daily ventilation period is substantially coordinated with the daily active period during a heating / cooling period for the home. Additionally, the method includes determining a target volume in compliance with the adopted ventilation standard and determining a flow rate for delivering the fresh air during the daily ventilation period. Moreover, the method includes monitoring an accumulated total ventilation volume of the delivered fresh air until the accumulated total ventilation volume is within a vicinity of a target volume.

The invention discloses a control method of a base station air conditioner. The control method comprises steps as follows: the outdoor environment temperature and the outdoor environment relative humidity are detected; when the outdoor environment temperature is lower than a first preset temperature and the outdoor environment relative humidity is smaller than a first preset value, the base station air conditioner is controlled to be in a ventilation cooling mode; when the base station air conditioner is in the ventilation cooling mode, a revolving speed of an indoor motor of the base station air conditioner is adjusted according to a temperature difference value between the indoor environment temperature and a set temperature so as to adjust the ventilation volume. According to the control method of the base station air conditioner, the revolving speed of the indoor motor can be adjusted automatically according to refrigeration load in a base station after the base station air conditioner is controlled to be in the ventilation cooling mode, the load of the motor can be reduced while the room temperature is met, the power consumption is reduced, and purposes of energy conservation and environment protection are achieved. The invention further discloses a control device of the base station air conditioner.

The invention provides a preparation method of an RB-SiC (Reaction Bonded Silicon Carbide) substrate reflector surface modification layer, and belongs to the technical field of thin film deposition. The method comprises the following steps: I, preparing filming conditions: mounting a Si target on a twin sputtering cathode of a filming machine, performing surface cleaning treatment on the RB-SiC substrate, fixing to a filming machine workpiece clamp, vacuuming the filming machine and roasting the RB-SiC substrate; II, performing surface treatment on the surface of the Si target of the sputtering cathode: introducing Ar gas into the sputtering cathode, and adjusting medium frequency power supply power to perform sputtering pre-treatment on the surface of the Si target; III, preparing the Si modification layer: increasing the medium frequency power supply power and Ar ventilation volume, and depositing the Si modification layer on the RB-SiC substrate by using a medium frequency magnetron sputtering technology. The preparation process is greatly simplified, the compactness of the film layer is high, the film is uniform, the polishing characteristic is obviously improved, and the optical quality of the surface of the polished substrate is greatly improved.

The invention relates to a carriage window air-flow state tracking device and experimental method. The method comprises the steps: a fan is turned on to circulate the air in a pipeline, a flue gas generator is turned on, the flue gas is released through a main pipe, a branch pipe, a flow regulating value, a buffer tube, a release tube and a releasing hole to carriage window, and the rotating speed of the fan is regulated to control the flow, so that the trace of the flue gas in the carriage is clearly visible; a camera shoots the released flue gas and saved the image to a computer, real-time flow state of the air in the carriage is directly displayed, and the wind direction at the window is judged; wind speed data collected by a wind-velocity indicator is recorded by a data collector and introduced into the computer, the entrance time and speed curves are drawn by the data collector, the ventilation volume at the carriage window is calculated hereby, so that the data is provided for wind field simulation and fire oxygen consumption calculation of following fire space, and the device and the method are important for fire state judgment in fire-fighting study.

The invention discloses a recombinant secretory escherichia coli high-density fermentation method. The method comprises the steps of saving original strain of constructed recombinant secretory escherichia coli; screening high-expression strain; conducting working strain saving on the screened high-expression strain; activating the working strain; transplanting the working strain into a shake flask to expand cultivation; inoculating a fermentation tank to carry out high-density fermentation; controlling oxygen dissolve conditions by the adjustment of tank pressure, ventilation volume, stirring speed and material supply speed; according to the changes of oxygen dissolve, a pH value and the like, adjusting fed-batch speed of a supplemented medium; after the strain grows to high density, adjusting strain growing speed by controlling the fed-batch speed of the supplemented medium, and cooling to carry out fermentation until the fermentation is finished. The recombinant secretory escherichia coli high-density fermentation method has the advantages of being high in objective protein expression probability, high in growth density, short in fermentation period, easy to operate, low in cost and significantly reducing the risks of abnormal strain caused by external strain entering.

The invention discloses a seed germination acceleration method, relates to a production method of crop seeds, and belongs to the field of seed treatment. The method is characterized by comprising the following steps: soaking seeds in a 0.5% copper sulfate aqueous solution for 15-20 minutes; washing the seeds after being taken with clear water, airing and placing in an environment with the ventilation volume of 20L / min, wherein the germination acceleration temperature is 25-28 DEG C, and the illumination of a phytotron in germination acceleration is performed in a light and dark alternation manner at a 4 hour interval; and after the treatment, covering the seeds with sand, and watering and icing, wherein the thickness sum of the seeds and covering sand and ice is 40-50cm. The method disclosed by the invention has the beneficial effects that the good seeds which are full without pest and disease damages are selected; the seeds are mixed with the water and the sand and subjected to the freezing stacking treatment, the resting stage of the seeds is effectively broken, the germination rate of the seeds and the emergence rate of a nursery are increased, the seeding quantity is reduced, and the reproduction coefficient is greatly increased; by adopting the technology, the nutrition resistance and disease / pest resistance of the seedlings are improved, and the self-epidemic prevention system of the seeds is mobilized.

The invention relates to the technical field of electrical distribution cabinets, and especially relates to an intelligent temperature-adjustable lighting distribution cabinet. The intelligent temperature-adjustable lighting distribution cabinet comprises a distribution cabinet shell, and a pressure sensor is mounted at the outer side of the lower end of the distribution cabinet shell. An energy-saving lamp is mounted in the middle of the top inner wall of the distribution cabinet shell. A photosensitive resistor is mounted at the left side of the top inner wall of the distribution cabinet shell, and the photosensitive resistor is arranged between the left side of the distribution cabinet shell and the energy-saving lamp. A controller is mounted at the right side of the top inner wall of the distribution cabinet shell. By using the pressure sensor and the photosensitive resistor, the energy-saving lamp can be turned on automatically while the door of the distribution cabinet is opened under weak light, so that users can operate the distribution cabinet easily in the evening; by using a blower and a temperature sensor, hot air can be blown when the temperature is too low and cold air can be blown when the temperature is too high, and therefore, the temperature of the distribution cabinet can be adjusted intelligently; and the ventilation volume can be adjusted intelligently by a shutter, and the shutter is opened completely at high temperature and closed at low temperature.

The invention discloses a method for regulating the molecular weight of schizophyllan as a fermentation product of schizophyllum commune franch. The method comprises the following steps: taking slopestrains of schizophyllum commune franch, and carrying out slope inoculation and culture by adopting a PDA culture medium test tube, thereby preparing fermentation seed liquid; inoculating the seed culture liquid into a fermentation tank for fermentation, wherein the initial stage of fermentation is 0-36 hours, and the fermentation conditions are controlled as follows: the stirring rotation speed is 100-400r / min, the ventilation volume is 0.20-0.8vvm, and the temperature is 26-28 DEG C; the middle stage of fermentation is 37-84 hours, and the later stage of fermentation is 85-96 hours; and respectively controlling the fermentation conditions, discharging after the fermentation is ended, and removing mycelia; concentrating, precipitating, filtering to obtain refined schizophyllan. Accordingto the method disclosed by the invention, polysaccharides are degraded by utilizing enzyme self-secreted by the thalli, so that not only is the procedure of degrading after separating and purifying crude polysaccharides with larger molecular weight and poorer solubility and obtained by fermentation in the traditional method greatly simplified, but also the product yield is increased and the production cost is reduced.

The invention relates to a composite bacteria liquid for cooperative degradation of petroleum and a preparation method thereof. The composite bacteria liquid is formed by mixing fermentation liquids of two strains of alcanivorax 97CO-5 and 97CO-6 and a strain of marinobacter PY97S wherein cell concentration is 107 to 109 CFU / mL. The preparation method comprises the steps of: inoculating seed liquids of the alcanivorax and the marinobacter respectively by 5% of inoculation quantity into an M8 culture medium or sodium acetate culture medium; fermenting and culturing under the conditions of 25 DEG C, the ventilation volume of 0.25m<3> / h, the stirring speed of 150rpm and defoaming by a fed defoamer; mixing all the fermentation liquids to prepare the composite bacteria liquid; centrifuging to prepare 5 times concentrate; and finally storing at 4 DEG C. The composite bacteria liquid is suitable for biodegradation of petroleum pollution at lower environmental temperature and thoroughly degrading alkane components and aromatic components in the petroleum, has higher petroleum degradation ratio than bacteria liquid with single degradation bacteria, has obvious cooperative degradation effect, and is applied to biological repair of coastlines or beaches polluted by ocean spilled petroleum.