The invention discloses a micro 
fluid control detection device based on surface-enhanced 
Raman scattering active substrate. The micro 
fluid control detection device is obtained by the method including the following steps: 
photoresist is coated on substrate in 
spinning way, prebaking, 
exposure, developing and fixing are sequentially carried out on the 
photoresist, so as to form 
photoresist graph in micro fluid shape; 
plasma dry etching is carried out on the photoresist, thus forming nano granular structure or nano 
fiber upright structure in vertical distribution on the substrate; the nano granular structure is a 
mask, 
anisotropic etching is carried out on the substrate, thus forming nano column on the substrate; 
metal nano granular layer is sputtered on the 
silicon nano column or nano 
fiber upright structure, so as to obtain surface-enhanced 
Raman scattering active substrate; and a 
silicon-PDMS double-layer structure SERS micro 
fluid control detection device which has no 
impurity interference and can be monitored in real time is formed by combining micro fluid device and 
processing technology thereof. The micro fluid control device not only can be used for detection of liquid 
analyte to be analyzed but also can be used for detection of 
colloid and gas 
analyte to be analyzed.