The invention belongs to the technical field of fine chemicals and particularly relates to a disinfection method and a 
disinfectant for 
medical equipment and a preparation method of the 
disinfectant.The 
disinfectant is prepared from 
eucalyptus oil, radix sophorae flavescentis, 
flos lonicerae, cortex magnoliae 
officinalis, radix glycyrrhizae, herba plantaginis, 
herb of 
veronica linariifolia, 
arisaema fargesii tuber, radix scutellariae, herba houttuyniae, pericarpium zanthoxyli, star anise, ginger, 
radix bupleuri, 
rhizoma coptidis, herba menthae, rhizoma ligustici, pericarpium granati, 
flos chrysanthemi, rubiae radix et rhizoma, fructus cnidii, glycerin, 
sodium bicarbonate and a surfactant. The disinfectant for the 
medical equipment is mainly prepared from 
traditional Chinese medicine decoctions as main raw materials, can effectively inhibit and kill 
bacteria and viruses such as 
hepatitis virus, 
Bacillus enteritidis, 
dysentery bacillus, 
escherichia coli, influenza 
virus and the like andhas a broad-spectrum 
antibacterial effect; meanwhile, with addition of the anionic surfactant, the disinfectant has a good cleaning effect on blood, sweat and other stains, cannot produce 
adverse effect on 
skin when used for a long time, is 
safer to use and removes the stains more thoroughly; the disinfectant does not foam and is convenient to use.