83 results about "Blueberry Root" patented technology

The invention discloses a method for rapid mycorrhization of a DSE strain and blueberry tissue culture seedlings, and belongs to the technical field of biology. A strain is R16, and the preservation number is CGMCC No. 13885. The invention also establishes the method for the rapid mycorrhization of the strain and the blueberry tissue culture seedlings. The system uses sterilized moss as a co-culture medium under an aseptic condition, and DSE inoculant adopts a liquid culture method. Compared with an existing method of inoculation by fungus lumps, the strain R16 cultivated by inoculated liquid grows fast and contacts with blueberry root fungus fully, the co-culture time is short and the planting rate is high. The DSE strain R16 has a significantly promoting effect on the growth of the blueberry tissue culture seedlings. Compared with the uninoculated control, the growth of the blueberry seedlings inoculated with DSE strain is significant, the leaves are large and dark green, the root system is developed and the survival rate of transplanting is high.

The invention relates to a blueberry mycorrhizal fungi (coprinus micaceus) and a separation method and application thereof, belongs to the technical field of microbiology, and mainly relates to a mycorrhizal fungi which separated from a Changbai Mountain wild cowberry root system for blueberry inoculation and application of the mycorrhizal fungi at promoting the growth of blueberry and improving the yield of the blueberry. The blueberry mycorrhizal fungi is preserved in the Chinese General Microbiological Culture Collection Center, and the classification name is Coprinus micaceus. By the application of the mycorrhizal fungi, the growth of the blueberry root system can be promoted. The invention has the advantages that the yield, fruit setting rate and insect damage prevention function can be improved, and the like.

The invention belongs to the technical field of plant tissue culture and particularly relates to a high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture. Primary inducing culture medium used in the method is improved WPM+Zt 1.0-1.5mg / L, successive multiplication culture medium is improved WPM+Zt 0.5-0.8mg / L, and rooting culture medium is 1 / 2 improved WPM+IAA 0.1-0.2mg / L+AC 0.5g / L. The method comprises the steps of: dipping the stem bases of successively multiplied aseptic blueberry seedlings in IBA sterile solution with concentration being 100-500mg / L for 5-15 seconds, inoculating the stem bases in the blueberry rooting culture medium, moving a rooting tissue culture seedling culture bottle from a culture room to a plastic greenhouse for training seedlings for 4-7 days after rooting, uncovering the plastic greenhouse and training the seedlings for 3-5 days before transplanting, transplanting the seedlings in culture medium in a cell tray, laying a plastic film to preserve moisture for 5-7 days, ventilating twice a day for 5-15 minutes per time during moisture preservation and fully removing the plastic film after 7 days. The high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture has the advantages that not only is the efficiency high, but also the cost is lower and the mass production is facilitated.

The invention relates to the technical field of industrialized seedling production and in particular relates to a blueberry tissue culture propagation and ex-vitro rooting method. The method mainly aims to solve the technical problems that rapid industrialized blueberry industrialized seedling production is restrained due to low rooting rate, long rooting period, complex rooting program, high rooting cost and the like during in-vitro blueberry rooting. The method comprises the following steps: selecting and sterilizing an explant, inducing lateral bud germination, performing multiplication culture, and finally performing ex-vitro rooting, wherein in the culture medium for inducing lateral bud germination, the components of the culture medium comprises an improved WPM basic culture medium, 0.8-2mg / L of ZT, 25-35mg / L of white sugar and 5-10g / L of agar; the pH value is regulated to be 5.4; according to the improved WPM basic culture medium, the original potassium sulfate and calcium chloride are replaced by calcium nitrate hydrate and potassium nitrate.

The invention relates to a method suitable for rapid rooting of blueberry tissue culture seedlings, which includes the steps: cutting off single blueberry tissue culture seedlings which are cultured on rooting medium for 40-50 days and grow to more than 6-8cm, soaking the seedlings in rooting agent, performing cuttage to insert cuttings into feeding block in each bottle, and allowing the seedlings to grow a plurality of roots at the base in 18-22 days; removing a tissue culture seal film from the rooted tissue culture seedlings growing to more than 6-8cm, exercising the seedlings for 30 days, and directly transplanting the seedlings to a greenhouse. By the method, blueberry rooting culture is performed using the feeding blocks in the bottles initially, proper rooting medium and best rooting agent are selected, and accordingly an efficient blueberry tissue culture seedling rooting system is established. The method has the advantages that operation is simple, cost is low, the method is stable and efficient, seedling cycle is shortened greatly, and the like.

The invention provides technology for grafting southern highbush blueberries with rabbiteye blueberries serving as stocks. According to the technology, a bud grafting method is adopted, the southern highbush blueberries are grafted with the rabbiteye blueberries serving as the stocks, so that the survival rate is high, the bud grafting speed is high, scions are saved, an operation method is simple, tree bodies of obtained new plants are high, life is long, the tree vigor is strong, adaptability to the soil conditions is high, resistance to blueberry root rot is high, fruits are attractive, seeds are small, taste is good, blueberries ripe early, and the technology is suitable for transformation of a large number of orchard varieties.

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention belongs to the technical field of fruit tree fertilizers and relates to a blueberry fruit tree dedicated fertilizer and a preparation method thereof. The blueberry fruit tree dedicated fertilizer using rabbit excrement as the main raw material is a solid organic fertilizer which is prepared from the following raw materials in mass percent: 40-60% of rabbit excrement, 30-50% of straws, 3-6% of nitrogenous fertilizer, 1.5-2% of phosphatic fertilizer and 1.5-2% of potassic fertilizer. The preparation method for the blueberry fruit tree dedicated fertilizer comprises the following steps: fermenting the mixture of the rabbit excrement and the straws, adding the nitrogenous fertilizer, the phosphatic fertilizer and the potassic fertilizer, culturing liquid mycorrhizal fungi, and the like, so that the blueberry fruit tree dedicated fertilizer provided by the invention is prepared. According to the blueberry fruit tree dedicated fertilizer, sulphur is not added, so that the soil pH is prevented from being excessively low or excessively high; the mycorrhizal fungi is added to promote growth of bacilli of the blueberry root system; according to the characteristics of the rabbit excrement raw material, the straws and other ingredients such as ammonium sulfate, monoammonium phosphate and potassium sulphate are added to ensure uniformity and stability of the product; the preparation method for the blueberry fruit tree dedicated fertilizer has the advantages that the process is simple, the raw material is easy to obtain, the rabbit excrement and straws are used as main raw materials, and the cost is low.

The invention discloses a blueberry root domain space covering cultivation method which comprises the steps as follows: adopting a planting furrow covering method or a planting hole covering method, covering the interior of the planting furrow with crop straws, sawdust or pine needles, laying microporous permeable non-woven fabrics on all walls and the bottom of the planting furrow, and using plastic films to partition the non-woven fabrics on all walls of the planting furrow, planting blueberry seedlings, wherein the root system of the blueberry seedlings cannot penetrate through the non-woven fabrics, filling the nutrient soil to the planting furrow and performing fix planting of the blueberry seedlings, and covering overground parts of the blueberry seedlings after fixing planting with the crop straws, sawdust or pine needles with certain height, wherein the pH value of soil is stabilized within the range of 4.5-5.7. Therefore, the blueberry root domain space covering cultivation method is simple to operate, the purpose of improving blueberry planting soil can be achieved only with lower cost, the influence on growth of blueberries due to inappropriate soil conditions is avoided, the soil has favorable permeability, the blueberries grow well, the fruit quality is improved, and the blueberry root domain space covering cultivation method is very conducive to production of organic fruit products and expansion of planting range of the blueberries.

The invention discloses blueberry rhizosphere growth-promoting bacillus cereus as well as a composite microbial agent and an application thereof. The strain is classified and named as bacillus cereus (Bacillus cereus) L5, is preserved in China General Microbiological Culture Collection Center on 31, May in 2013 and has the preservation number of CGMCC No.7667. The invention also provides an application of the bacillus cereus, a microorganism composite microbial agent comprising the bacillus cereus, and a preparation method of the composite microbial agent. The blueberry rhizosphere growth-promoting bacillus cereus L5 disclosed by the invention can be successfully colonized in blueberry rhizosphere soil, and growth of blueberries can be promoted. According to the microorganism composite microbial agent disclosed by the invention, the optimized combination of the blueberry rhizosphere growth-promoting bacillus cereus L5, bacillus fusiformis L13, lactobacillus casei and other strains is realized, so that the blueberry composite microbial agent has high pertinence and effectiveness.

The invention discloses a novel soilless blueberry cultivation method. The novel soilless blueberry cultivation method mainly includes the steps of container selection, medium selection and supporting drip irrigation facility construction, wherein in container selection, since blueberries belong to shallow-rooted plants and root systems of the blueberries are vertically distributed at 10-50 cm, root control devices with the diameter of 80 cm and the height of 40 cm are selected, and planting spacing is 1*2 m; in medium selection, coconut chaff with an EC value smaller than 0.8 mS / cm serves as a culture medium and is covered with rice chaff 10 cm in thickness, and blueberry seedlings are planted after the coconut chaff is soaked; in supporting drip irrigation facility construction, drip irrigation pipes 16-20 mm in diameter are adopted above the root control devices. The novel soilless blueberry cultivation method is capable of accelerating blueberry root growth remarkably, increasing the blueberry yield, controlling weeds and saving labor cost.

The invention discloses a soil improvement method for improving cultivation survival rate of blueberry in north. The soil improvement method comprises the following steps: in the spring one year before blueberry seedling cultivation and planting, testing the soil pH value; when the soil pH value is bigger than 5.5, spreading a compound fertilizer containing sulphur powder into soil, so as to decrease the pH value, and when the soil pH value is lower than 4.0, spreading a compound fertilizer containing lime powder into soil, so as to increase the pH value; after each time of fertilizer spreading, shallowly ploughing soil at the depth of 15-35 cm, so as to uniformly mix the fertilizer with soil and finally enable the soil pH value to reach 4.0-5.5. According to the soil improvement method, as the blueberry root system is shallow, the compound fertilizers containing organic substances are spread into the shallow soil layer at the depth of 0-35 cm, so that the soil aeration, water permeability and drainage property are improved; improved soil is suitable for blueberry to survive and grow.

The invention discloses biological organic fertilizer suitable for blueberry plantation. 40-80 parts of cow dung and 10-30 parts of sawdust are mixed and then are fermented by bacillus subtilis at a high temperature for 3-10 days, 20-50 parts of rape dreg cake is added into the fermentation product, a small amount of urea, potassium sulfate, disodium hydrogen phosphate and sodium selenite are added into the mixture, and the mixture is uniformly stirred and fermented by saccharomyces cerevisiae at a normal temperature for 3-6 days so that a finished product is obtained. Through use of a large amount of weed seeds in high temperature decayed cow dung and high temperature fermentation, burnt blueberry root is effectively prevented and cow dung odor is fast removed. Through secondary fermentation, a urea substance loss is avoided in high temperature fermentation, efficiency of conversion of inorganic nitrogen into organic nitrogen is improved, and fertilizer long residual action is guaranteed. Through use of a large amount of organic matters such as cow dung, sawdust and rape dreg cake containing a large amount of cellulose, a soil structure is improved and blueberry root rapid growth is promoted.

The invention discloses a pezicula ericae strain with a plant growth promoting effect and application thereof. The pezicula ericae functional strain 3-41 belongs to Dermateaceae in Bacteroidetes. Thetaxonomy name of the strain is pezicula ericae strain 3-41 (Pezicula ericae being equal to Cryptosporiopsis ericae). The strain is preserved in Guangdong province microbe bacterial preservation centeron June 1, 2017, and has the preservation code being GDMCC No.60283. The pezicula ericae functional strain 3-41 is obtained through being separated from wild blueberry root systems; after the strainis inoculated into the culture variety of the blueberry 'Lanyu' pot plant seedlings, the blueberry root systems can be infected; the strain mainly infects the skin and the cortical layer cells; the mode belongs to a typical endophytic fungi fixed planting mode. In addition, the blueberry potted plant inoculation tests show that the inoculation strain 3-41 obviously promotes the growth of the overground parts of the planted blueberries; the pure culture can be realized; the industrial production can be easily realized; good application prospects are realized.

Disclosed is a blueberry root growth promoting method employing a nutritive pot for seedling raising by cutting. The blueberry root growth promoting method includes (1) preparing a cutting matrix for seedling raising; (2) preparing active root developing solution; (3) arranging small arc greenhouses in a large greenhouse to manage cutting slips; (4) subjecting the cutting slips to the normal management after rooting. The blueberry root growth promoting method has the advantages that the rooting of the cutting slips is good, survival rate of the blueberry is improved, and after stored in the sands and subjected to inverted inserting process, the blueberry managed in the greenhouse can synchronously root.

The invention relates to a preparation method for carbazolyl isopropanolamine derivative and application thereof. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwifruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteriaand the like.

The invention discloses a soil acid adjustment method used for blueberry plantation. According to the soil acid adjustment method, dilute sulphuric acid is packaged with composite material coated fiber membrane bags (sustained-release membrane bags), and is placed close to the root of saplings, so that molecules are released and conducted into soil gradually via coating fiber, and requirements of blueberry on pH value of cultivating soil are satisfied, wherein the composite material coated fiber membrane bags are penetrated by the coating fiber. Sustained release effect of the coated fiber membrane enables that pH value of blueberry root local environment can be adjusted in time, durability can be achieved via supplement of the packaged liquid, and the soil acid adjustment method is convenient, economical, and long-acting. The soil acid adjustment method is capable of adjusting soil pH value to a reasonable range required by blueberry rapidly and conveniently; local soil acid adjustment improvement benefits are achieved as far as possible, so that waste is reduced; utilization effect is long lasting and excellent; and when the packaged liquid becomes insufficient, supplement can be realized via bottle openings.

The invention discloses blueberry root system inducing nutritious soil for growing seedlings. The blueberry root system inducing nutritious soil for growing seedlings comprises the following components by weight percent: 30-70% of dry sphagnum, 10-30% of high-temperature dried chicken manure particles, 10-50% of high-temperature dried cow manure particles, 5-10% of seaweed meal, 3-5% of protein fermentation substance and 1-5% of potassium humate, wherein the seaweed meal is normal-temperature dried undaria pinnatifida powder; the protein fermentation substance is prepared by aerobically fermenting 20-80% of egg white and 80-20% of sesame-seed cake at normal temperatures. The blueberry root system inducing nutritious soil for growing seedlings has the advantages that the nutritious soil formula can meet the best blueberry growth requirements on soil breathability, nutrient slow-release capability, acidity and alkalinity, and nutrient content; meanwhile, the nutritious soil formula has functions of increasing the diameter of fine roots of the blueberry, especially inducing the growth of a root system with a diameter class being 3mm.

The invention discloses a soil conditioner for blueberry cultivation. The soil conditioner comprises the following components in parts by weight: 100-120 parts of a bioorganic fertilizer, 80-90 parts of fermented bean residue, 20-30 parts of furfural residue, 40-45 parts of turf, 8-13 parts of humic acid, 10-15 parts of moss, 3-5 parts of functional polypeptide, 15-18 parts of a water-retaining agent, 150-180 parts of an auxiliary agent and 10-30 parts of a conditioning agent. The soil conditioner has the beneficial effects as follows: the furfural residue is used for substituting sulfur, so that the cost is reduced, the environment is improved and the pH value of soil is significantly reduced; a functional polypeptide calcium complex can inhibit calcium absorption of a blueberry root system and prevent iron-deficiency chlorosis or death of blueberry; the soil conditioner has the effects of fixing carbon, retaining water, reducing the soil bulk density, increasing the porosity and the like, is high in organic matter content, helps to increase the seed germination rate, promote the root system growth and delay root system aging in the later stage, and is a high-efficiency and environment-friendly soil conditioner for the blueberry cultivation.

The invention discloses a highly-efficient biological bacterial fertilizer for blueberry, and a preparation method and an application thereof. The highly-efficient biological bacterial fertilizer forthe blueberry is prepared by using 15 parts of a microbial agent, 30 parts of an organic fertilizer, 8 parts of a nitrogen fertilizer, 10 parts of a phosphorus fertilizer, 12 parts of a potassium fertilizer, 5 parts of a microelement fertilizer, 3 parts of chitosan, 3 parts of amino acid, 3 parts of cytex, 6 parts of humic acid and 5 parts of bentonite as raw materials through mixing under stirring, sieving, drying and packaging. The highly-efficient biological bacterial fertilizer provided by the invention is applied to the following aspects: increase of the yield of the blueberry, improvement of the quality of the blueberry, enhancement of the storage performance of the blueberry, induction of reinforcement of disease resistance of the blueberry, promotion of the growth of a blueberry plant, improvement of the microorganism number of a blueberry root system and the enzyme activity of soil, etc. The highly-efficient biological bacterial fertilizer provided by the invention has the advantages of obvious effects, greenness, no pollution, high fertilizer utilization rate, soil improvement, prevention and control of diseases and pests, yield and quality improvement, low cost, easinessin popularization, etc.

The invention relates to a 1, 3, 4-oxadiazole hydrazide compound as well as a preparation method and application thereof. The compound has a structure as shown in a general formula (I), according to the invention, the method includes taking a 1, 3, 4-oxadiazole compound as a basis and introducing hydrazide into the system; the compound has a good inhibition effect on plant pathogenic bacteria, fungi and oomycetes, and has a good inhibition effect on pathogenic bacteria such as rice bacterial leaf blight, wheat scab, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, phytophthora infestans, blueberry root rot and the like.

The invention belongs to the field of traditional Chinese medicines and provides a pharmaceutical preparation for treating pelvic inflammation. The pharmaceutical preparation is prepared from the following raw materials: 20 parts of campsis grandiflora, 16 parts of Indian azalea leaves, 15 parts of rough knotweed herbs, 20 parts of lobedfruit tacca leaves, 20 parts of fragile blueberry roots, 15 parts of serrateleaf carallia herbs, 18 parts of chun spicebush roots, 20 parts of common oxyspora roots, 16 parts of Chinese shuteria roots and 20 parts of okubo dryoathyrium frond. The pharmaceutical preparation can be used for treating pelvic inflammation.

The invention relates to heterocyclic substituted 1, 3, 4-oxadiazole hydrazide compounds and a preparation method and application thereof. The compound has a structure shown as a general formula (I). The compound has a relatively good inhibition effect on pathogenic fungi and bacteria; and the compound has good inhibition effects onfusarium graminearum, potato late blight bacteria, blueberry root rot bacteria, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, botryosphaeria dothidea, rhizoctonia solani, xanthomonas oryzae pv., ralstonia solannacearum, Xanthomonas citri, kiwi canker bacteria, Xanthomonas oryzae cucumber, Xanthomonas oryza konjak, Botryosphaeria dothidea, tomato canker pathogens, apple canker pathogens and the like.

The invention discloses special bio-fertilizer for blueberries as well as a preparation method and an application of the bio-fertilizer. The bio-fertilizer is prepared from raw materials including 10parts of a Bacillus cereus agent, 5 parts of a bacillus subtilis agent, 5 parts of a trichoderma agent, 30 parts of organic fertilizer, 8 parts of nitrogen fertilizer, 10 parts of phosphate fertilizer, 12 parts of potash fertilizer, 5 parts of trace fertilizer, 4 parts of plant growth promoting substances, 6 parts of humic acid and 5 parts of bentonite; the raw materials are stirred, mixed, sieved, dried and packaged, and the special bio-fertilizer for the blueberries is prepared. The special bio-fertilizer is used for increasing blueberry yield, improving blueberry quality, improving blueberry storing property, induced-enhancing disease resistance of the blueberries, promoting growth of blueberry plants, increasing microbe quantity of blueberry root systems and improving soil enzyme activity, and has the advantages of being remarkable in effect, green, pollution-free, high in fertilizer use ratio, low in cost, easy to popularize and the like, soil is improved, diseases are controlled,and yield and quality are improved.

The invention discloses a Chinese medicinal preparation for treating facial neuritis. The facial neuritis is known as 'wry mouth' in Chinese medicine and is caused by insufficient anti-pathogenic energy, wind pathogen which enters middle collaterals by taking the advantage of a weak point or wind phlegm and blood stasis which block venations to cause that the qi and blood of yangming meridians of the head and face do not run smoothly and then cause the facial neuritis. Thus, the Chinese medicinal preparation is prepared from the Chinese herbal medicines of Chinese holly grass, herb of common herons bill, rhizoma typhonii, rigescent hawksbeard herb, radix ligustici sinensis, viola muehldorfii, acuteleaf caper root, fragile blueberry root, scorpion, caulis polygoni multiflori, herba ajugae, purple loosestrife and gynura divaricata which have the effects of expelling wind and clearing away cold, dredging the channels and activating blood circulation to dissipate blood stasis, wherein the Chinese herbal medicines are decocted into a decoction with water in a manufacturing laboratory. Clinical tests show that the total effective rate of the Chinese medicinal preparation reaches 97.4% and is obviously superior to a comparison group, so that the Chinese medicinal preparation is worthy of clinical popularization and application.

The invention provides a traditional Chinese medicine for assisting development during B-ultrasonography and a preparation method thereof. The traditional Chinese medicine for assisting development during B-ultrasonography is prepared from the following raw herbal materials: raphanus sativus, maire yew seeds, ferula asafetida, pyropolyporus fomentarius teng, java marsh pennywort, knotweed, orchidaceae oberonia iridifolia, notholirion bulbuliferum, ribes davidii franch, ferula borealis, crab shells, sculellaria barbata, gourian clematis, delavay blueberry root, common bombax flower, wight anotis, mallow and sagittaria guayanensis. The traditional Chinese medicine provided by the invention has the beneficial effects the traditional Chinese medicine for assisting development during B-ultrasonography is low in cost, easy to use and safe and has no toxic or side effect, good development ratio can be improved, and inspection accuracy can be improved, so that more gastrointestinal diseases can be detected through B-ultrasonography, and a strong basis is provided for clinical diagnosis; meanwhile, a preparation technology is simple and practical.

The invention discloses a rhizosphere growth-promoting Bacillus arbihattai specially used for blueberries and its application. The strain is named Bacillus aryabhattai, numbered BPR078, and has been preserved in China Microorganisms on January 7, 2016. General Microbiology Center of Species Preservation Committee, the deposit number is CGMCC No.11971. The blueberry rhizosphere growth-promoting Bacillus arbii of the present invention is inoculated into blueberry cutting seedlings after being prepared as bacterial agent, which can obviously accelerate blueberry rooting and promote the growth of cutting seedlings, and the difference reaches a significant level compared with the control. Therefore, the present invention provides excellent bacterial strain resources for the future development of blueberry-specific microbial fertilizers.

The invention relates to application of 4'-demethyl epipodophyllotoxin as an agricultural bactericide. The compound has a relatively good inhibition effect on pathogenic bacteria and fungi; the invention aims at rice bacterial leaf blight, tobacco bacterial wilt, cucumber bacterial leaf blight, konjac bacterial leaf blight, citrus canker pathogen, grape canker pathogen, tomato canker pathogen, kiwi canker pathogen, apple canker pathogen, cucumber botrytis cinerea, pepper fusarium wilt pathogen, sclerotinia sclerotiorum, wheat gibberellic disease, potato late blight pathogen, and blueberry root rot bacteria, and the compound has a good inhibition effect.

The invention discloses a traditional Chinese medicine patching agent for treating hyperostosis. The hyperostosis belongs to the range of paralysis and heumatism according to clinical manifestation and onset age. According to inducements of wind, cold, dampness, heat and strain, loaded inflammatory pathological changes of bones are caused, so that disease symptoms such as qi and blood loss, blood stasis and stagnation and unsmooth main and collateral channels are caused. The medicine is prepared by the following steps: selecting traditional Chinese medicines such as eclipta, diversifolious osmanthus branchlet leaf and bark, Clinacanthus nutans, fragile blueberry root, pyrola, Yunnan begonia herb, scabrous doellingeria root, pedunculate acronychia wood or root, Centranthera cochinchinensis, red psychotria root and Japanese pagodatree bark, with the effects of warming liver and kidney, activating blood circulation and dispersing blood stasis, relaxing muscles and tendons and removing obstruction from meridians, softening bony spur and eliminating cold and dampness, adding vinegar, distilled spirit and water, thereby obtaining the patching agent. According to the clinical test in the institute, the total effective rate is 97.4 percent, and the curative effect is good.