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82 results about "Candida krusei" patented technology
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Candida krusei is a budding yeast (a species of fungus) involved in chocolate production. Candida krusei is an emerging fungal nosocomial pathogen primarily found in the immunocompromised and those with hematological malignancies. It has natural resistance to fluconazole, a standard antifungal agent. It is most often found in patients who have had prior fluconazole exposure, sparking debate and conflicting evidence as to whether fluconazole should be used prophylactically. Mortality due to C. krusei fungemia is much higher than the more common C. albicans. Other Candida species that also fit this profile are C. parapsilosis, C. glabrata, C. tropicalis, C. guillermondii and C. rugosa.
Detection method of invasive fungus infection, detection kit and application
ActiveCN109055502ASimplify detectionSimplify operabilityMicrobiological testing/measurementDNA/RNA fragmentationCell freeFluorescence
The invention provides a fast multiplex PCR identification diagnosis detection method of invasive fungus infection based on cfDNA (cell free DNA). The method can be used for identifying the invasive infection caused by clinic common and high-incidence Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida glabrata and Aspergillus fumigtus. An amplification primer is designed according to characteristic genome segments of each fungus category and species; a detection fluorescence probe of a strain can be distinguished according to the amplification segment design; the real time PCR can be performed on a sample to be tested; high sensitivity of nested PCR and high specificity and multi-target performance advantages of multiple fluorescent hybrid probe PCR are integrated for identifying the fungus strain. The invention also provides a PCR diagnosis kit for the invasive fungus infection and application thereof.
Owner:DIASYS DIAGNOSTIC SYST SHANGHAI
Antibody for detecting aspergillus
ActiveCN101149377AEasy to operateStrong specificityFused cellsBiological testingPenicillium marneffeiCandida famata
This invention provides the capture antibody and the detection antibody to detect the aspergillus, the two antibody is the single clone antibody of the cross oncocyte CCTCC NO.C200731 and CCTCC NO.C200730, they can unites the glucoprotein whose molecular weight is 25-100kDa in the cell wall of the aspergillus, the reaction with the Penicillium marneffei, white oidiomycosis, smoothness oidiomycosis, tropic oidiomycosis, close smoothness oidiomycosis, Candida krusei are all negative. The antibody in this invention can make into the immunity reagent to detect the aspergillus in common use.
Owner:ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
Compound microbial decomposed inocula, and preparation method and application thereof
ActiveCN104789490AGood synergyEfficient decompositionFungiBio-organic fraction processingFermentationAspergillus niger
The invention relates to compound microbial decomposed inoculas, and a preparation method and an application thereof. Effective components of the involved compound microbial decomposing inocula comprise bacillus amyloliquefaciens Ba168, paenibacillus peoriae BC-39, trichoderma harzianum, candida krusei, aspergillus niger and streptomyces griseus. The preparation method comprises that each strain are fermented separately, and the strains are mixed for use according to the proportion after the completion of the fermentation. The involved application is an application of the compound microbial decomposing inocula in degradation of crop straw. The compound microbial decomposing inocula not only give full play to unique degradation function of the bacillus on the straw, are combined with microbial compound flora for efficient synergy in the straw degradation process, thereby efficiently and rapidly degrading the straw, and significantly enhancing soil organic matter and humus levels.
Owner:杨凌绿都生物科技有限公司
Microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period
ActiveCN101658243AImprove immunityImprove disease resistanceAnimal feeding stuffBiotechnologyDisease
The invention relates to microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period, which belongs to the production field of feed additives.A preparation method of the microbiological feed additive comprises the following steps: adding inorganic selenium source into a microbial inoculum culture medium to ensure that the concentration of selenium in the culture medium is 2 to 10 mg / kg; inoculating Candida krusei, Candida valida and Streptococcus thermophilus seed liquor in the culture medium according to the ratio of 13:1:2; mixing theculture solution with the a proper amount of corn flour, and drying at 35 to 40 DEG C to obtain the feed additive. The invention adopts the principle that probiotics can convert sugar into glycerin and convert inorganic selenium into organic selenium to produce the complex probiotic product which is rich in glycerin and organic selenium, can exert the efficacies of glycerin, organic selenium andprobiotics simultaneously, can supply energy to ruminant, regulate and control the energy metabolism, adjust intestinal flora, resist pathogenic microorganism, increase appetite, enhance body immunityand disease resistance, improve the production performance, and is a safe and novel feed additive with has high value and no residual.
Owner:NANJING AGRICULTURAL UNIVERSITY
Novel voriconazole broad-spectrum antifungal medicine compound, broad-spectrum antifungal medicine composition and application thereof
ActiveCN101575330ABroad and potent antifungal activityImprove mechanical propertiesOrganic active ingredientsAntimycoticsMonilinia laxaItraconazole
The invention relates to a novel broad-spectrum antifungal medicine compound, a broad-spectrum antifungal medicine composition, an application of the compound or the composition in the preparation of a broad-spectrum antifungal medicine, and an application of the compound or the composition in the preparation of a medicine used for treating severe invasive infection (comprising Candida krusei) caused by invasive aspergillosis and / or Fluconazole-resistant Monilia and / or severe infection caused by Scedosporium and fusarium. The novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition have extensive and strong antifungal activity and better dynamic property and safety. For deep mycotic infection, the novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition are better than the original voriconazole in the aspects of the antibiotic activity and the medicine resistance and is better than amphotericin B in the aspects of safety and effectiveness; and compared with the formerly applied voriconazole, Fluconazole, itraconazole and amphotericin B, the novel broad-spectrum antifungal medicine compound and the broad-spectrum antifungal medicine composition have reinforced medicine effect, less adverse reaction and good safety.
Owner:LIVZON PHARM GRP INC
Kit used for detecting candida krusei in intestinal tract by fluorescence quantitative PCR method
InactiveCN101555523ARapid Quantitative DetectionTrue reflection of colonizationMicrobiological testing/measurementMicroorganism based processesFluorescenceHigh flux
The invention relates to a kit, and aims at providing a kit used for detecting candida krusei in the intestinal tract by a fluorescence quantitative PCR method. The kit comprises a standard positive template and two primers of a specificity amplification candida krusei ITS zone used in the PCR amplification process, wherein the gene of a coded amplification product has the nucleotide sequence shown by SEQ ID NO: 3. The operation is simple and convenient and fast; the specificity is good, and the sensitivity is high; the detection based on nucleotide is not limited by culture conditions; the quantitative detection can truly reflect the situations of candida krusei permanent planting and infection in the intestinal tract; and high-flux sample detection can be implemented simultaneously. The kit can carry out fast and quantitative detection to the candida krusei in the intestinal tract, can replace the traditional diagnostic method of isolated culture which is used for a long time, and is suitable for being widely popularized and applied in clinical laboratory.
Owner:ZHEJIANG UNIV
Candida categorized detection primer and probe composition, kit, detection method and application of composition
ActiveCN109971883AImprove featuresHigh sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesFluorescenceCandida famata
The invention provides a Candida categorized detection primer and probe composition, a kit, a detection method and an application of the composition. The Candida categorized detection primer and probecomposition comprises specific primers, detection probes and complementary probes which are used for Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata, and the complementary probes and the detection probes are incompletely complemented and paired. The primer and probe composition can detect and determine presence and absence of Candida infection onceand can distinguish the Candida albicans, the Candida tropicalis, the Candida parapsilosis, the Candida krusei and the Candida glabrata in the same fluorescent light channel.
Owner:DYNAMIKER BIOTECH TIANJIN
Pharmaceutical compositions and methods to vaccinate against disseminated candidiasis
InactiveUS20060083750A1Treating preventingInhibit bindingAntibacterial agentsFungiCell adhesionAdjuvant
The invention provides a vaccine including an isolated Als protein family member having cell adhesion activity, or an immunogenic fragment thereof, with an adjuvant in a pharmaceutically acceptable medium. The invention also provides a method of treating or preventing disseminated candidiasis. The method includes administering an immunogenic amount of a vaccine an isolated Als protein family member having cell adhesion activity, or an immunogenic fragment thereof, in a pharmaceutically acceptable medium. A method of treating or preventing disseminated candidiasis also is provided that includes administering an effective amount of an isolated Als protein family member having cell adhesion activity, or an functional fragment thereof, to inhibit the binding or invasion of Candida to a host cell or tissue. The Als protein family member can be derived from a Candida strain selected from the group consisting of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata and Candida parapsilosis and the Als protein family member includes Als1p, Als3p, Als5p, Als6p, Als7p or Als9p.
Owner:LOS ANGELES BIOMEDICAL RES INST AT HARBOR UCLA MEDICAL CENT
Pharmaceutical compositions and methods to vaccinate against disseminated candidiasis and other infectious agents
InactiveUS20070077256A1Treating preventingInhibit bindingAntibacterial agentsBacterial antigen ingredientsCell adhesionAdjuvant
Owner:LOS ANGELES BIOMEDICAL RES INST AT HARBOR UCLA MEDICAL CENT
Traditional Chinese medicine composition for preparing Candida resisting medicines
ActiveCN104083361AHigh antibacterial activityGood synergyAntimycoticsAldehyde active ingredientsCandida famataCandida glabrata
The invention relates to a traditional Chinese medicine composition for preparing Candida resisting medicines. The traditional Chinese medicine composition contains the ingredients in parts by weight: 40-80 parts of cinnamaldehyde and 30-60 parts of pogostone. The traditional Chinese medicine composition for preparing the Candida resisting medicines, provided by the invention, can be used for treating deep or systemic fungal infections caused by Candida, including deep or systemic fungal infections caused by Candida albicans, Candida tropicalis, Candida krusei or Candida glabrata. According to the traditional Chinese medicine composition, the pogostone is screened from numerous ingredients of ageratum oil, has relatively high bacteriostasis activity and has a good synergistic effect with the cinnamaldehyde; the cinnamaldehyde and the pogostone are suitable for being used after being combined; compared with the bacteriostasis activity after the cinnamaldehyde and the ageratum oil are combined, the bacteriostasis activity after the pogostone and the cinnamaldehyde are combined is remarkably improved, and the safety is good.
Owner:THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
Method for producing 1,3-propanediol and 2,3-butanediol from raw starch material
The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g / L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g / L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.
Owner:TSINGHUA UNIV
Fungus rapid culture, identification and susceptibility detection kit and detection method thereof
ActiveCN101886111AGrowth inhibitionGrow fastMicrobiological testing/measurementCandida famataAntibiotic Y
The invention discloses a fungus rapid culture, identification and susceptibility detection kit for rapidly and effectively identifying multiple fungi and testing the susceptibility. The invention also discloses a method for directly detecting a clinical sample by using the kit. The kit has the advantages that the multiple fungi are simultaneously identified and different pores are favorable for growing different fungi by coating various sugars or inhibitors on a susceptibility test board, so that the most common fungi in clinic use, such as candida albicans, candida tropicalis, candida glabrata, candida krusei, cryptococcus neoformans, candida guilliermondii and the like, can be identified and effective guidance is provided for the clinical use. The detection method is simple and suitable for detecting pure fungi, can be further directly applied to fungus identification of the clinical sample and the susceptibility detection of antibiotics and has a short detection period, namely thefungi to be detected can be simultaneously identified and the susceptibility can be detected at one time and the result can be given in 24 hours; and thus the detection method greatly shortens the clinical detection period and is favorable for early diagnosis and early therapy of patients.
Owner:AUTOBIO DIAGNOSTICS CO LTD
Candida krusei fluorescence PCR detection kit
ActiveCN106048051AEasy to operateStrong interference abilityMicrobiological testing/measurementFluorescenceNucleotide
The invention relates to a candida krusei fluorescence PCR detection kit. The candida krusei fluorescence PCR detection kit comprises CD-PCR reactant liquor, the CD-PCR reactant liquor comprises a primer and a fluorescent probe A, the primer is divided into a primer F and a primer R, the nucleotide sequence of the primer F is as shown in SEQ ID No.1, the nucleotide sequence of the primer R is as shown in SEQ ID No.2, and the nucleotide sequence of the fluorescent probe A is as shown in SEQ ID No.3. The invention further relates to a method for detecting candida krusei. The kit has the advantages of high capacity of resisting disturbance, high sensitiveness, high specificity, high detection speed, convenience in operation and the like.
Owner:泰普生物科学(中国)有限公司
Candida with nematocidal activity as well as preparation method and application of candida
ActiveCN104263664AGood nematicidal activityNo pollution in the processBiocideFungiMicroorganismMicrobial pesticide
The invention provides Candida with a nematocidal activity as well as a preparation method and application of Candida and belongs to the technical field of microbial pesticides. The Candida is Candida krusei TY9 and is preserved in the typical culture preservation center in China, and the preservation number of the Candida is CCTCCNO: M2014435. The Candida krusei TY9 strain is prepared by fermentation culture of conventional liquid, is applied to prevention and control of plant-parasitic nematodes, and particularly has a good effect of preventing and controlling panagrellus redivivus and / or bursaphelenchus xylophilus. The Candida has the advantages of low cost, simple preparation process, easiness in raw material obtaining, good in effect of killing nematodes and the like, and is suitable for industrial popularization and application.
Owner:NANYANG NORMAL UNIV
Application of combination of tacrolimus and fluconazole in preparing antifungal drugs
InactiveCN103520157AReverse drug resistanceHigh antibacterial activityOrganic active ingredientsAntimycoticsAntifungal drugsCandida famata
The invention discloses application of combination of tacrolimus and fluconazole in preparing antifungal drugs. According to the invention, static and dynamic combined antifungal effects of combination of tacrolimus and fluconazole to common non-albicans candida species are researched by various methods. The researches show that combination of tacrolimus and fluconazole has a remarkable synergic antifungal effect to common non-albicans candida species (candida glabrata and candida krusei). Predictably, combination of tacrolimus and fluconazole can be used for preparing antifungal drugs. According to the invention, tacrolimus and fluconazole are applied in a combined way to enhance the antimicrobial activity of fluconazole to non-albicans candida species so as to generate the synergic antifungal effect. Combination of tacrolimus and fluconazole can reverse the drug resistance of non-albicans candida species to fluconazole, thereby providing a research direction for development of new drugs and new use of old drugs.
Owner:QIANFOSHAN HOSPITAL OF SHANDONG
Method for producing 1,3-propanediol and 2,3-butanediol from raw starch material
ActiveUS20090081745A1Decrease seed culturing periodFermentationClostridium pasteurianumConcentrations glucose
The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g / L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g / L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.
Owner:TSINGHUA UNIV
LAMP (loop-mediated isothermal amplification) kit for early diagnosis of candida krusei and special primer
ActiveCN105950740AIncrease reaction rateHigh amplification efficiencyMicrobiological testing/measurementDNA/RNA fragmentationOperabilityLoop-mediated isothermal amplification
The invention relates to an LAMP (loop-mediated isothermal amplification) kit for early diagnosis of candida krusei and a special primer. The primer is designed by using an ADE2 sequence of a candida krusei strain as a target sequence. After proofing by effectivity, specificity and sensitivity experiment, the primer has the characteristics that the primer is used for the early diagnosis of candida krusei infection; the rapidity, simplicity and convenience are realized, the operability is strong, and the result judging is convenient; the problems of long diagnosis period, higher requirement on techniques of detectors and conditions of laboratory platforms, etc. in the clinical candida krusei diagnosis are solved; the primer can be prepared into the kit, and can be used for early diagnosis of candida krusei infection in clinical application.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Biological decomposer and preparation method thereof
InactiveCN102765815AInhibitory activityEasy to separateBiological water/sewage treatmentFiberLactobacillus acidophilus
The invention provides a biological decomposer and a preparation method thereof. The biological decomposer is characterized by comprising the following components in part by weight: 1 to 2 parts of lactobacillus acidophilus, 0.5 to 1.5 parts of candida krusei, 0.5 to 1 part of photosynthetic bacteria, 0.5 to 1 part of actinomyce, 6 to 8 parts of brown sugar, 1 to 2 parts of yeast extract, 2 to 3 parts of pineapple juice and 90 to 200 parts of water. The activity of spoilage organisms can be inhibited, organisms in water are promoted to decomposition, organic matters in wastewater and sewage are subjected to enzymolysis by decomposed biogroups, macromolecule organic matters are decomposed into micromolecule organic matters capable of being dissolved in water, the enzymolysis products can enter biological cells and participate in the biological reaction in the cells, and are further decomposed under the action of organized ferments, so a textile plant with a macromolecule structure is decomposed into a fibrous matter with a micromolecule structure.
Owner:HAO YUAN TE CONTROL
Use of rhizoma paridis chinensis for preparing antifungal drug
The invention discloses use of rhizoma paridis chinensis for preparing an antifungal drug, belonging to the technical field of medicines. In-vitro cell experiments show that rhizoma paridis chinensishas different levels of antifungal effects and even fungus-killing effect to drug-resistant candida albicans 103, candida albicans SC5314, cryptococcus neoformans, candida krusei, candida parapsilosis, candida glabrata and candida tropicalis and therefore can be taken as the antifungal drug. According to the use, new use of rhizoma paridis chinensis is opened up, rhizoma paridis chinensis is usedfor preparing the antifungal drug, and a new candidate drug is provided for the treatment of fungal infection. Currently, drugs with wide antifungal spectrums and fungus-killing activity on clinic arevery few, and a new way is provided for the clinical and efficient treatment of infection of fungi and even drug-resistant fungi by virtue of the characteristics of wide antifungal spectrums and fungus-killing activity of rhizoma paridis chinensis under the conditions that clinical fungus drug resistance becomes common increasingly and the drug resistance level becomes serious increasingly.
Owner:SHAANXI UNIV OF SCI & TECH
DPO primer pair, detection method and kit of candida classification detection and application of kit of candida classification detection
InactiveCN109811080AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationEscherichia coliNucleotide
The invention provides a DPO primer pair, detection method and kit of candida classification detection and application of the kit of candida classification detection. Nucleotide sequences of the DPO primer pair are as shown in SEQ ID NO.1-2. The primer pair can specifically amplify candida, and is not crossed with other common pathogenic bacteria such as aspergillus, escherichia coli and staphylococcus aureus, moreover, by designing the DPO primer pair, products of different candida strains have different annealing temperatures, through melting curve analysis of the products, candida albicans,candida tropicalis, candida parapsilosis, candida krusei and candida glabrata can be judged, only one pair of primers are used for achieving multiple detection, and compared with the prior art, the DPO primer pair is more simple, convenient and economic.
Owner:DYNAMIKER BIOTECH TIANJIN
An extract for regulating female vaginal microecology
The invention discloses a traditional Chinese medicine composition for adjusting female vaginal microecology. The traditional Chinese medicine composition comprises: be weight, 1 to 2 parts of licorice, 1 to 2 parts of clove, 1 to 2 parts of common cnidium fruit, 1 to 2 parts of pomegranate rind, 1 to 2 parts of Chinese gall, 1 to 2 parts of giant knotweed, 1 to 2 parts of Chinese magnoliavine fruit, 1 to 2 parts of anemone root, 1 to 2 parts of coptis root, 1 to 2 parts of amur corktree bark, 1 to 2 parts of aucklandia root and 1 to 2 parts of raw stemona root. The traditional Chinese medicine composition can be utilized directly for a female vagina to remove effectively pathogenic bacteria in the female vagina. The traditional Chinese medicine composition can kill staphylococcus aureus,staphylococcus epidermidis, streptococcus faecalis, escherichia coli, bacillus proteus, candida albicans, candida tropicalis, candida krusei and other candidas simultaneously.
Owner:NINGXIA MEDICAL UNIV
Vaccine for veterinary and human medicine prophylaxis and therapy
The present invention relates to a novel vaccine, its use for immunoprophylaxis and / or the treatment of candidamycoses in human and veterinary medicine as well as methods for its preparation, wherein said vaccine consists of the combination of the Candida strainsa1) Candida albicans CCM 8355a2) Candida glabrata CCM 8356a3) Candida krusei CCM 8357 anda4) an immunomudulating Propionibacterium acnes strain,and optionally pharmaceutically acceptable excipients, such as carriers, wherein the ratio of the components a1-a4 in the end product is a1:a2:a3:a4 is 10-20:10-20:10-20:40-70.
Owner:VRZAL VLADIMIR +1
Candida drusei LSA and application thereof in ammonia-nitrogen degradation
ActiveCN104877920AThe cultivation method is simpleFast growthFungiMicroorganism based processesYeastActive component
The invention discloses a candida drusei LSA and an application thereof in ammonia-nitrogen degradation. The preservation registration number of the candida drusei LSA disclosed by the invention is CGMCC NO.10562. The invention further discloses a culture of the candida drusei LSA, and further discloses a bacterial suspension of the candida drusei LSA. The invention further discloses a product; and the active components of the product are the candida drusei LSA, the culture or the bacterial suspension. The product has the following function: (a) degrading ammonia and nitrogen, or (b) degrading ammonia and nitrogen in water, or (c) degrading ammonia and nitrogen in excrements, or (d) reducing the ammonia emission of the excrements. The candida drusei disclosed by the invention can be applied to the following aspects: (1) improvement of water quality problem of ammonia-nitrogen pollution, (2) improvement of indoor environmental health when the candida drusei is applied to livestock and poultry houses, and (3) application as a feed additive for chicken.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Primer group and kit for simultaneously detecting multiple pathogenic fungi
ActiveCN112522434AStrong species specificityImprove detection efficiencyMicrobiological testing/measurementDNA/RNA fragmentationFonsecaea pedrosoiMultiplex
The invention discloses a primer group and kit for simultaneously detecting various pathogenic fungi. The primer group comprises 14 pairs of primers, and the primers can perform specific multiplex amplification on DNA sequences of 14 target fungi, wherein the target fungi comprise candida albicans, candida tropicalis, candida glabrata, candida parapsilosis, candida krusei, aspergillus fumigatus, aspergillus terreus, trichophyton mentagrophytes, trichophyton rubrum, epidermophytom floccosum, sporothrix schenckii, microsporum caninum, fonsecaea pedrosoi and malassezia fufur. The kit adopts a multiple fluorescence labeling PCR amplification technology for multiplex amplification, multiple common pathogenic fungi can be identified at the same time through a one-time reaction, the detection efficiency is high, the speed is high, and the cost is low; in addition, interspecific and intraspecific comparison can be performed on sequences in a fungus ITS or beta-tubulin database, an intraspecific conserved region is selected to design an amplification primer, and very strong species specificity is achieved. The whole detection process can be completed within 2-3 hours, the detection speed ishigh, and the requirement for rapid detection of clinical samples can be met.
Owner:THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
Application of ovateleaf knotweed rhizomes in preparing antifungal drugs and synergist thereof
ActiveCN109700858AReduce concentrationExhibits synergistic antifungal actionOrganic active ingredientsAntimycoticsCandida famataMonilinia
The invention discloses an application of ovateleaf knotweed rhizomes used for preparing antifungal drugs and a synergist thereof, belongs to the technical field of medicine. In vitro cell experimentsshow that ovateleaf knotweed rhizomes have different degrees of antifungal and even fungicidal effects against drug-resistant Candida albicans 103, susceptible Candida albicans SC5314, Cryptococcus neoformans 32609, natural drug-resistant Candida krusei ATCC 2340, Candida parapsilosis 22019, natural drug-resistant Candida glabrata ATCC 2281 and drug-resistant Candida tropicalis, so that the ovateleaf knotweed rhizomes can be used as an antifungal drug and a synergist thereof. The invention opens up a new use for ovateleaf knotweed rhizomes, uses the ovateleaf knotweed rhizomes for preparing antifungal drugs, and provides a new drug candidate for the treatment of fungal infections.
Owner:SHAANXI UNIV OF SCI & TECH
LAMP (loop-mediated isothermal amplification) primer composition for detecting Candida krusei, LAMP detection kit based on LAMP primer composition, and application method of LAMP detection kit
InactiveCN106521009AStrong strain specificitySmooth responseMicrobiological testing/measurementDNA/RNA fragmentationFluorescencePolymerase L
The invention discloses an LAMP (loop-mediated isothermal amplification) primer composition for detecting Candida krusei, a detection kit based on the primer composition, and a method for detecting Candida krusei; the kit comprises the LAMP primer composition, BstDNA polymerase, dNTP, 10*Isothermal Amplification reaction buffer solution, MgSO4, H2O and a color developing agent; the color developing agent includes fluorescent dye SYBGreen or fluorescent indicator Syto 9. The LAMP primer composition of the invention designs four primers at specific endogenous gene of Candida krusei, and has high cultivar specificity so that smooth reaction is guaranteed. In addition, the kit of the invention also has the advantages of good operation simplicity, high reaction speed, high reaction efficiency, high specificity and sensitivity, good identification process diversity and the like, and is suitable for clinical popularization and application.
Owner:SHANGHAI EAST HOSPITAL +2
Method for treating <125>I medical wastewater through active yeast enrichment
InactiveCN102311175ASolve oxygenSolve energy shortageBiological water/sewage treatmentBottleC. tropicalis
The invention relates to a method for treating <125>I medical wastewater through active yeast enrichment, which is mainly used for the field of clinical radioactive wastewater. In a bottle inserted with an injection needle head and added with 2 percent of brown sugar liquid, candida tropicalis, candida krusei and mixed bacteria of the candida tropicalis and the candida krusei are incubated for 48hours, and when the bacterial number is greater than 460*108 / mm<3>, the adsorption culture with <125>I-deoxyribose nucleic acid (DNA), <125>I-T3, <125>I-T4 and <125>I-radio immunoassay (RIA) medical wastewater is carried out for 24 hours. The primary saturation adsorption of the three kinds of yeast on four kinds of markers is 83.0 percent to 99.8 percent and 54.0 to 410.0 mg.g<-1>, wherein the optimum effect of the mixed bacterium adsorption on <125>I-RIA is 99.8 percent and 410 mg.g<-1>, and the adsorbed compound analysis is 10.8 percent to 12.7 percent through three-time washing. The radioactive wastewater after adsorption treatment has shallow color and no peculiar smell, the supernatant liquor CPM is smaller than 100, the radioactive activity is smaller than 10 Bq / L, and the nationalradioactive wastewater discharge standard is reached. The <125>I medical wastes through enrichment recovery is in a dry powder shape, the size is reduced by 100 times, and the continuous placement and the yeast autolysis prevention are favorably realized.
Owner:刘枫晨 +1
Preparation method of biological full-degradation kitchen waste FDM microbial inoculum
The invention relates to a preparation method of a biological full-degradation kitchen garbage FDM microbial inoculum. The preparation method comprises the following steps: step 1, raw material preparation: culturing bacillus cereus fermentation liquor, aspergillus oryzae fermentation liquor, micromonospora purpurea fermentation liquor, candida krusei fermentation liquor and acetobacter aceti fermentation liquor, wherein the effective viable count in each single component in the microbial inoculum is high, the kitchen waste can be completely and quickly degraded only by a small amount of the microbial inoculum, the degradation rate is up to 96%, the amount of wastewater generated after the kitchen waste is fully degraded by the FDM microbial inoculum is small, granular precipitates are few, and up-to-standard discharge can be realized through a simple water treatment process.
Owner:上海滢弘环保科技有限公司
Preparation method of melastoma penicillatum extract, bacteriostatic operation method and application of melastoma penicillatum extract
ActiveCN111249320AGood antibacterial effectConsistent with the mechanism of actionAntimycoticsPlant ingredientsBiotechnologyCandida sp
The invention discloses a preparation method of a melastoma penicillatum extract, a bacteriostatic operation method and an application of the melastoma penicillatum extract. The preparation method provided by the invention can be used to obtain a water extract and an alcohol extract of melastoma penicillatum. The bacteriostatic operation method provided by the invention has the advantages of goodstrain activation effect and high bacteriostatic operation accuracy. The extract disclosed by the invention can be used for resisting fungi, and is particularly suitable for being applied to resistingcandida albicans Ca, candida tropicalis Ct, candida glabrata Cg, candida krusei Ck and candida parapsilosis Cp. The melastoma penicillatum extract provided by the invention has an obvious inhibitioneffect on candida albicans and the like, and can lay a certain foundation for research and development of melastoma penicillatum.
Owner:HAINAN MEDICAL COLLEGE
Antibacterial compound and application thereof
ActiveCN104817619ALow inhibitory concentrationAntimycoticsTripeptide ingredientsAntifungalAspergillus fungus
The invention discloses an antibacterial compound and application thereof. The formula of the compound is showed in formula (I). The method is characterized in that bacillus subtilis with the ZJU007 CGMCC No. 4140 is used to obtain a lead compound Chlorotetaine, a drug delivery process is used as the theoretical basis to perform homologous modeling so as to build the virtual structure of small molecule peptide transport proteins, the virtual structure of the small molecule peptide transport proteins is used to optimize the structure of the lead compound Chlorotetaine to obtain a tripeptide compound with a fungus resisting capability. The antibacterial compound has the advantages that the compound can resist candida funguses such as candida albicans, candida parapsilosis, candida krusei and polymorphic candida, and minimal inhibitory concentration (MIC) can reach 0.81 microgram / mL; the compound can also resist aspergillus funguses such as aspergillus niger, and MIC can reach 1.63 microgram / mL.
Owner:ZHEJIANG UNIV
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