434 results about "Microbial pesticide" patented technology

The invention relates to a composite microbial fertilizer for controlling crop diseases and insect pests, which contains composite microbial fungicide and is prepared by the following raw materials based on parts by weight: 10-35 parts of composite microbial fertilizer fungicide, 3-15 parts of composite microbial pesticide fungicide, 1-5 parts of insecticidal synergist, 1-5 parts of plant growth regulator, 20-60 parts of grass carbon, 5-15 parts of expanded chicken manure, 5-50 parts of inorganic fertilizer and 1-3 parts of binding agent, wherein the composite microbial fertilizer fungicide is prepared by bacillus megaterium and jelly-like bacillus; and the composite microbial pesticide fungicide is prepared by mixing bacillus thuringiensis and bacillus subtilis, adding sodium percarbonate type solid state oxygen-increasing agent for mixing and fermenting and finally absorbing by the grass carbon. The composite microbial fertilizer adopts strains with good suitability, and the strains are compounded according to a certain proportion to obtain the composite fungicide which has durable activity and stable performance; and the four beneficial strains are compatible with each other and performs the own functions, so that the multiple aims of resisting germs as well as diseases and insect pests, and increasing the crop yield can be achieved.

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention discloses a Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof. The Bacillus siamensis strain is separated from peels of Laiyang pears with sterilized surfaces, the strain has broad-spectrum resistance for various types of phytopathogens and has a good effect on preventing and treating the pear fruit rot caused by pear ring rot and soft rot. The strain comes from pear fruits and is safe to pear fruits; the strain is produced on a large scale, is low in raw material cost, convenient to operate and stable in prevention and treatment effect, and can effectively solve the problems of agricultural residues and environment pollutions caused by pear fruit disease and insect pesticide prevention and treatment. The strain has huge application potential on the aspects of biological prevention and treatment of after-harvest diseases and insects of pears, and the excellent basic strain is provided for research and development of microbial pesticides.

The invention relates to a method for preparing a microbial fertilizer, a nutrient solution, a fodder and a pesticide and for fermentation bed cultivation by utilizing indigenous microorganism probiotics. The microorganism probiotics are formed by fermenting eight pure natural materials, including indigenous bacteria, green juice, fresh fish amino acid, enzyme alcohol, Chinese prescription nutrient solution, lactic acid bacteria, natural calcium and water-soluble calcium phosphate potassium; the microorganism probiotics contain the mixed flock of various beneficial bacteria, such as, lactic acid bacteria, photosynthetic bacteria, bacillus and yeast; the fertilizer can be used for fertilizing and loosening soil; the agricultural products are free from residue, taste nice and are high in yield; the fodder can be used for guaranteeing no residue on the cultured animal meat product; the meat is tender and free from smell; after the microorganism nutrient solution is used, the microorganism pesticide can prevent and treat insect diseases; the chemical pesticide and the fertilizer residue can be eliminated and decomposed; when the fermentation bed is used for culturing, the excrement cleaning is not required; the animal excrement is decomposed by the fermented padding while beneficial mycoprotein is formed; the fodder can be eaten by the animals; the microbial fertilizer is odorless, zero-emission, environment-friendly, pollution-free to environment and beneficial to the health of human and animals.

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

A wettable powder of bacillus thuringiensis and beauveria bassiana relates to a mixed biopesticide, aiming to the shortcomings of prevention and treatment limitations of beauveria bassiana and bacillus thuringiensis (Bt), particularly poor quick-acting property of microbial pesticides. The wettable powder provided in the invention is prepared from the following components: 10-14wt.% of bacillus thuringiensis, 4-14wt.% of beauveria bassiana, 3-5wt..% of wetting agent, 10-20wt.% of dispersant, 0.1-0.5wt.% of ultraviolet protectant and 50-60 wt.% of carrier. The wettable powder of bacillus thuringiensis and beauveria bassiana provided in the invention is high in activity, stable in effect, long in persistence and convenient for use.

The invention discloses bacillus subtilis S001 capable of inhibiting a variety of pathogens and further discloses a microbial preparation prepared from the bacillus subtilis S001 and a preparation method the microbial preparation. The bacillus subtilis S001 with the collection number of CGMCC No.6425 has the function of promoting growth and inhibiting pathogens including the fusarium, pepper rhizoctonia solani, tomato leaf mold, tomato early blight pathogen, tomato grey mold, eggplant sclerotia pathogen, wheat full-rotting pathogen, wheat root rotting pathogen and wheat sheath blight pathogen. The microbial pesticides and microbial fertilizers prepared from the bacillus subtilis S001 are environmentally-friendly and have good safety, and pathogens can not resist the microbial pesticides and the microbial fertilizers easily.

The invention discloses bacillus methylotrophicus wettable powder which comprises 100 parts by mass of bacillus methylotrophicus active components, 4-10 parts by mass of a wetting agent and 4-10 parts by mass of a dispersing agent. A method for preparing the bacillus methylotrophicus wettable powder comprises the following steps: weighing the bacillus methylotrophicus active ingredients, the wetting agent and the dispersing agent according to a ratio, and uniformly mixing; and milling through a jet mill, thereby obtaining the bacillus methylotrophicus wettable powder. The wettable powder is stable in quality and convenient to preserve and use for a long time, has good effects of preventing and treating cucumber gray mold and apple rot, solves the problems such as environmental toxic and side effects and pesticide residues brought by chemical agents, has the characteristics of high efficiency, no toxicity, safety and no residue, is a safe, effective, low-residue and environment-friendly microbial pesticide and has wide application prospects in the field of microbial control.

The invention relates to a bacillus licheniformis which can prevent and treat plant disease and promote plant growth, and a low-cost preparation method of fungicide, belonging to the technical field of microbial pesticides and microbial fertilizers. The fungicide is an efficient plant growth-promoting bacteria-bacillus licheniformis B4 bacterial strain separated and screened from aquatic plant rhizosphere soil, and is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6677. In the invention, bacillus licheniformis is cultured in large scale by using sweet potato starch wastewater as a fermentation culture medium to prepare the fungicide which can prevent and treat plant disease and promote plant growth. The process is low in preparation cost and simple to operate, and also can realize resource utilization of the sweet potato starch wastewater and reduce pollutant discharge. The prepared fungicide, used for preparation of tea leaves and vegetables, can not only improve the yield, but also improve the quality of tea gardens and vegetables, so that the bacillus licheniformis has wide application prospect.

The invention relates to a method for recyclingly producing a trichoderma sp. biological control agent from potato starch waste water and mushroom residues and belongs to the technical field of microbial pesticides and environmental protection. The method adopts high-concentration organic wastewater produced in potato starch production and mushroom residues as main medium raw materials and utilizes a liquid state-solid state combined fermentation production method to produce the trichoderma sp. biological control agent. The method comprises the following steps of 1, inoculating potato starch wastewater as a culture medium with a trichoderma sp. strain and carrying out liquid-state culture to obtain a liquid strain, 2, inoculating potato starch waste water as a fermentation raw material with the liquid strain to obtain a large number of liquid trichoderma sp. products, 3, adding the cultured liquid strain or the liquid trichoderma sp. products into mushroom residues, and carrying out solid state fermentation to obtain solid trichoderma sp. products, and 4, carrying out drying, crushing and packaging of the solid trichoderma sp. products. The method for producing trichoderma sp. biological control agent has the characteristics of low production cost and good environmental benefits. The trichoderma sp. biological control agent obtained by the method can control a plurality of fungal diseases of plants. The method provides an effective treatment approach for high-efficiency treatment on potato starch wastewater and mushroom residues, and reduces environmental pollution.

The invention relates to a bacillus amyloliquefaciens which can prevent and cure plant disease and promote plant growth, and a low-cost preparation method of fungicide, belonging to the technical field of microbial pesticides and microbial fertilizers. The fungicide is an efficient plant growth-promoting bacteria-bacillus amyloliquefaciens (Bacillus amyloliquefaciens) A3 bacterial strain separated and screened from aquatic plant rhizosphere soil, and is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6676. In the invention, bacillus amyloliquefaciens is cultured in large scale by using sweet potato starch wastewater as a fermentation culture medium to prepare the fungicide which can prevent and cure plant disease and promote plant growth. The process is low in preparation cost and simple to operate, and can also realize resource utilization of the sweet potato starch wastewater and reduce pollutant discharge. The prepared fungicide, used for preparation of tea leaves and vegetables, can not only improve the yield, but also improve the quality of tea gardens and vegetables, so that the bacillus amyloliquefaciens has wide application prospects.

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug / ml of cadusafos. The fungus has good application and development prospects.

The invention relates to a method for using soybean root nodule and endotrophic mycorrhiza to prevent soybean root disease. Wherein, the inventive bacteria seed has been stored in national microbe storage manager center, whose storage number is sinorhizobium sp; the seed L396 storage number is CGMCC No. 1851; the bacillus subtilis seed Snb2 storage number is CGMCC No.1850. And the invention uses general liquid to ferment and cultivate, uses itself or metabolite as effective active component; mixing the ferment solutions at different ratios, with effective restrain function on soybean cyst nematization and root pathogenic fungi; the density ratio between Snb2 and L392 is 8:3, and the dead ratio of young bacteria can reach 98.92%, and the relative restrain rate on cyst cultivation can reach 99.66%; the invention prepares the metabolite into water agent, to reach 78.6% prevent rate on the soybean cyst nematization. The inventive nodule bacteria can fix nitrogen and accelerate growth.

The invention relates to bacillus subtilis with a stronger phosphorus decomposing effect and stronger gamma-polyglutamic acid producing capacity. Furthermore, the bacillus subtilis has a better preventing and treating effect on varieties of plant diseases and a plant growth promoting effect. A preservation serial number of the bacillus subtilis JCD-H-16 with phosphorus decomposing, disease preventing and growth promoting effects disclosed by the invention is CGMCC NO.13663, a preservation date is February 16th in 2017, and the bacillus subtilis has multiple effects of decomposing phosphorus, preventing diseases, promoting growth, high producing gamma-polyglutamic acid, increasing yield, improving quality, having a wide antibacterial spectrum and the like. A bacterial strain and a fungicideof the bacillus subtilis disclosed by the invention can effectively prevent and treat powdery mildew, bacterial wilt, gray mold, rice sheath blight disease, pimento anthracnose, damping off and the like; broad-spectrum antibacterial is achieved, and effects are stable and durable; the bacillus subtilis can be applied to developing a bacterial fertilizer and a microbial pesticide preparation integrated with phosphorus decomposing, gamma-polyglutamic acid producing and disease preventing and treating. When the bacterial fertilizer of the bacillus subtilis disclosed by the invention is applied,about one half of a nitrogen fertilizer and a phosphorus fertilizer can be reduced, and an obvious yield increasing effect is further obtained.

The invention discloses a bacillus amyloliquefaciens and a microbial inoculum, and a production method and application of the microbial inoculum, and belongs to the field of microorganisms. The bacillus amyloliquefaciens (CGMCC (China General Microbiological Culture Collection Center) No.13047) has the disease prevention and growth promoting capability. The bacillus amyloliquefaciens with biological activity is inoculated in a solid substrate to be fermented; an obtained fermentation product can be directly used as a bacillus amyloliquefaciens microorganism inoculants; the microbial inoculum has the bacteriostasis effect and can be used as a microbial pesticide; phoma foveata, colletotrichum coccodes, fusarium avenaceum and fusarium chlamydosporum can be inhibited; the bacteriostasis rate is 67 percent or higher, so that the defects of poor environmental protection property, easy generation of drug resistance and poor safety of the chemical pesticide in the prior art can be overcome; the advantages of good environmental protection property, no drug resistance and high safety are realized.

The invention relates to burkholderia cepacia, and a cultivation method and an application thereof, and belongs to the field of plant protection (microbial pesticide). A preservation number of the burkholderia cepacia is CGCC No. 5384. According to the cultivation method, fermentation conditions are as follows: an LB culture medium is used; liquid volume is 50 mL; 1 mL of 2-day old seed strain is added; and the strain is cultured for 20-24 h at a temperature of 25-30 DEG C and at a rotation speed being 200 r / min. The burkholderia cepacia has significant inhibition effects for rhizoctonia solani, pyricularia oryzae, collectotrichum musae, fusarium oxysporum f. sp. cubense, gibberella zeae, peronophythora litchi, ceratocystis adiposum, fulvia fulva, xanthomonas oryzae pv. oryzae, xanthomonas oryzae pv. oryzicola and the like, can be used for biological control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environment friendliness and the like, and has wide application prospects.

The invention relates to a cultivation of epiphyte that has plant infestation wireworm poisoning function and the metabolite manufacture method and the application. The strain of the epiphyte is Asipergillus. niger snf0407009 and the conserving code is CGMCC No.1714. It is made up from normal liquid fermenting and cultivating, and its metabolite has high poisoning function to plant infestation wireworm, especially root wireworm. The influence to the Meloidogyne incognita has been measured indoor. Different thickness fermenting liquid would have obviously influence to Meloidogyne incognita. One time fermenting liquid has the similar function as 10ug / mL aldicarb. It has good application prospect.

The invention relates to a biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof, belonging to the technical field of microbial pesticides. The biocontrol microorganism is a Bacillus methylotrophicus Mo-Bm strain preserved in a CGMCC (China General Microbiological Culture Collection Center) on 12th October 2010 with a preservation number of CGMCC No.4207. A 16S rRNA gene sequence (with a sequence number of HQ325853 in a GeneBank database) of the Mo-Bm strain is a main characteristic basis for identifying the strain; and the Mo-Bm strain can resist 0.5-2 percent (V / V) carbinol, and is a secondary characteristic basis. The microorganism and metabolins thereof are prepared into a Mo-Bm wettable powder biocontrol anti-bacteria agent together with pesticide conventional absorption carriers and aids. The microorganism has a control effect of above 70 percent on tobacco black shank, cruciferclubroot and pseudo-ginseng root rot. The biocontrol microorganism has the characteristics of low cost, high control effect, no residue and safety to people and livestock, and is an efficient biological pesticide for controlling fungus diseases during organic agriculture planting.

The invention belongs to the technical field of biological pesticide, in particular to screening and identification of a coniothyrium minitans strain with strong parasitism and saprogenicity on sclerotinia sclerotium and an application thereof in a microorganism agent. The coniothyrium minitans ZS-1SB strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number being CCTCC NO: M209114. The invention also discloses an open-type solid fermentation cultivation method of the microorganism agent and an application of the coniothyrium minitans ZS-1SB microbial inoculum in the prevention and treatment of sclerotinia of colza. The microorganism agent of the invention has excellent biological prevention effect on plant fungous diseases such as sclerotinia rot of colza and the like.

The invention relates to bidirectional burkholderia as well as a culture method and an application thereof and belongs to the field of plant protection (microbial pesticide). The preservation number of the bidirectional burkholderia is CCTCC NO: M2013145. The culture method of the bidirectional burkholderia comprises the following steps: adding 1ml of 2-day-aged seed bacterial strain to an LB (Lysogeny broth) culture medium with the liquid loading of 50ml under a fermentation condition, and culturing for 20-24 hours at the temperature of 25-30 DEG C at the rate of 200r / min. The bidirectional burkholderia has a remarkable inhibition effect on rice sheath blight disease, rice blast, colletotrichum musae, oxysporum, fusarium asiaticum, peronophythora litchi, sugarcane alternaria tenuis, cladosporium fulvum, xanthomonas oryzae, bacterial leaf steak and the like, can be used for the biological prevention and control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environmental friendliness and the like and has a bright application prospect.

The invention discloses a bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of the bacillus methylothophicus strain. The endophytic bacillus methylothophicus strain is separated from dendrobium huoshanense roots which are subjected to surface sterilization, and the strain has the broad-spectrum resistance on multiple plant pathogens and has the good prevention and control effect on pear fruit rot caused by the ring rot and the soft rot. The endophytic bacterium serving as a biocontrol bacterium has the advantages of the broad-spectrum bacterial resistance, the efficient safety and the like and is safe to pear fruits, low in large-scale production raw material, convenient to operate, stable in prevention and control effect and capable of effectively solving the problems of residual pesticides and environmental pollution which are brought by pesticide prevention and control over pear fruit diseases. Accordingly, the huge application potential on biological disease prevention and control after pear harvesting is achieved, and a good basic bacterial strain is provided for researching and developing microbial pesticides.

The present invention belongs to the technical field of agricultural microbe, and specifically relates to a microbial capsule preparation taking poly-gamma-glutamic acid as a wall material, a preparation method thereof, and applications thereof in preparing microbial pesticides and microbial fertilizers. The microbial microcapsules taking the poly-gamma-glutamic acid as the wall material have good mechanical strength and flexibility, can be applied in microbial embedding medium for good sustained-release ability, can prevent microbial cells from injuries of ultraviolet rays, temperature, acid or base, ionic strength and heavy metal ions, can prevent microbial cells from damages in a production process, and have the advantages of effectively extending the shelf life of the preparation and the like. The microbial microcapsule preparation of the present invention can prepare different microbial microcapsule preparations according to different microbial species, and can effectively control capsule wall thickness and diameter of the microcapsules in the preparation process. The microcapsules of the invention have an embedding rate of more than 90% for microbial cells, and can realize industrial mass production.

The invention relates to nematicidal combination comprising paecilomyces lilacinus and carbosulfan. The combination comprises the paecilomyces lilacinus and the carbosulfan. The weight rate of the paecilomyces lilacinus to the carbosulfan is 100-1000 : 1-4, wherein the quantity of living bacterium of the paecilomyces lilacinus is 1-100 hundreds of millions cfu / g. The two pesticides in the combination of the invention are synergy in the prevention of the nematode, and the chemical pesticide and the microbial pesticide jointly act well. The invention realizes the action of simultaneously preventing the nematodes and the other pests by implementing the drug for one time. The combined use of the two pesticides greatly reduces the single use quantity of the pesticide.

The invention discloses a Brevibacillus laterosporus strain N106-2 of which the collection number is CGMCC No.8482. The strain has strong indoor flat inhibiting actions on multiple plant pathogenic fungi (fussarium wilt of tomato, root rot of chili, gray mold of tomato, Rhizoctonia solani, black spot of cabbage, anthracnose of pear and black rot of pear) and plant pathogenic bacteria (bacterial wilt of tomato, fire blight of pear and streak of rice); and the strain can secrete proteinase, cellulase and surfactant antimicrobial related substances with hemolytic activity. The Brevibacillus laterosporus can be prepared into a biocontrol aqua; the biocontrol aqua has certain preventing and treating effects on fussarium wilt of tomato, fusarium rot of chili, anthracnose of pome and eyespot of pome. The Brevibacillus laterosporus N106-2 has the advantages of no toxicity, no pathogenicity and high safety for human and livestock, is environment-friendly, is a useful resource for development of microbial pesticides, and has potential application prospects.

The invention provides a bacterial biological fungicide and a preparation method thereof. Microbial pesticide living Pseudomonas putida is used as a starting strain; and the bacterial biological fungicide is obtained after fermentation and reproduction. The bacterial biological fungicide can be used for nearly all crops infected by Pythium, Rhizoctonia and Fusarium, is safe for the crops, and has good effect on the crops such as cucumber, tomato, scallion, eggplant, green pepper, cotton, watermelon, sesame and the like. The bacterial biological fungicide has the greatest advantages that the bacterial biological fungicide is all nature, harmless, safe to people and livestock and environment-friendly; the pathogenic bacteria are not easy to produce resistance; and the bacterial biological fungicide is favorable for production of green vegetables, and has huge economic, ecological and social benefits.

The invention discloses a method and a device for microbial pest control based on light luring. The method for microbial pest control based on light luring includes the steps: luring and gathering target pests by a light source or frequency spectrum waves; applying entomopathogenic microorganisms with selective toxicity to the target pests at fixed points; enabling the target pests to carry the entomopathogenic microorganisms. The device for microbial pest control comprises a solar panel, an automatic powder sprayer, a trap lamp tube, a storage battery and a support, wherein the solar panel is fixed to the top end of the support, the automatic powder sprayer is suspended at the top end of the support, and the trap lamp tube is mounted at the center under the automatic powder sprayer. Powder spraying aerosol is mounted in the automatic powder sprayer and contains propellants and particle powder containing microbial pesticides. Light luring and microbial control are cooperatively applied, control area is enlarged by the aid of migratory flight of the pests, and influence on beneficial insects is reduced as the entomopathogenic microorganisms with the selective toxicity are applied according to the target pests.

The invention discloses a penicilliumgriseofulvum XJ-EP-058 strain as well as a microbial preparation resisting banana vascular wilt and application thereof, belonging to the technical field of microbial pesticides. The strain is penicilliumgriseofulvum XJ-EP-058 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5467. The microbial preparation, containing the strain, for resisting the banana vascular wilt is obtained by test tube seed culture, liquid seed culture for the strain and liquid fermentation culture for the strain. The strain and the microbial preparation, containing the strain, for resisting the banana vascular wilt have obvious in-vitro and in-vivo preventing effects and have the germination inhibition rate reaching 100% to spores of banana vascular wilt bacteria, and the average diameter of bacteriostatic zones to the banana vascular wilt bacteria is 14.5mm; and a result of a greenhouse potted living organism test shows that the strain and the microbial preparation have the average control effect reaching 74.38% on the banana vascular wilt bacteria. The microbial preparation has the advantages of low raw material cost and simple and feasible production process.

The invention discloses bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew and belongs to the field of microbial pesticides. The strain is (bacillus subtilis)FBR1 with a collection number of CGMCC No.4915. The industrial fermentation culture medium for the bacillus subtilis is prepared from 0.8 percent of corn flour, 1.5 percent of soybean flour, 0.1 percent of yeast powder, 0.5 percent of peptone, 0.8 percent of monopotassium phosphate, 0.15 percent of calcium carbonate, 0.02 percent of magnesium sulfate and 0.001 percent of defoamer, and by the steps of adjusting the pH value to 7.0, fixing volume by using water, and fermenting for 24 to 36 hours under conditions of a fermentation temperature of 28 to 35 DEG C, a ventilation volumeof 0.2 to 0.4 v / v / min and a stirring speed of 100 to 150r / min. The invention also discloses a bio-control preparation which contains bacillus subtilis and can be used for preventing and controlling powdery mildew in economic crops such as melons, vegetables, tobacco and strawberry in place of spray chemical medicines without polluting the environment.

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.