146 results about "Gelatin microspheres" patented technology

The invention provides a preparation method of a mono-dispersed gelatin embolic microsphere with a precisely-controlled particle size. In the provided method, micro water-in-oil liquid drops are formed in a micro channel reactor at first through a micro-fluidic liquid drop control technology, then a crosslinking agent and a gelatin water solution are taken as the dispersing phase and introduced into the water-in-oil system, and then the mixture is directly cured to form microspheres. By adjusting the flowing speeds of the continuous phase and the dispersing phase, and the flowing speeds of two dispersing phases, the particle size and sphericility can be precisely controlled. The preparation technology is simple and rapid, and the process is controllable. The prepared gelatin microsphere can be used as an embolic agent or an antitumor drug carrier, and can also be applied to cell culture.

The invention provides a porous titanium alloy human cervical interbody fusion cage with bioactivity and a preparation method thereof. The method comprises the following steps: firstly, inputting model data into electron beam melting equipment according to a design requirement to prepare a porous titanium alloy human cervical interbody fusion cage; secondly, preparing gelatin microspheres; and immersing gelatin microsphere dry powder in an rhBMP-2 solution for gelatin coating modification, preparing a gelatin solution A in double distilled water, immersing the porous titanium alloy human cervical interbody fusion cage in the gelatin solution A, mixing the rhBMP-2 gelatin microspheres and absolute ethanol to obtain suspension B, and immersing the gelatin-coating-modified porous titanium alloy human cervical interbody fusion cage in the suspension B to prepare the porous titanium alloy human cervical interbody fusion cage internally containing an rhBMP-2 sustained-release system. The cervical interbody fusion cage prepared with the method has modulus of elasticity close to that of natural bone tissues, and the porous structure and the bioactivity factor sustained-release system inside the cervical interbody fusion cage can induce growth of new bone tissues, so that the binding problem of bone-material interfaces is solved. Therefore, the cervical interbody fusion cage has bettermechanical compatibility and bone integration capability than those of a compact material.

A pharmaceutical microsphere comprises a bioactive agent and a biological carrier that encapsulates the bioactive agent, wherein the biological carrier is crosslinked with a crosslinking agent.

The invention discloses a three-dimensional porous chitosan / gelatine microsphere, a preparation method thereof and an application thereof in liver cell culture. The preparation method thereof comprises the following steps of: rapidly freezing mixture into spheres by dropping liquid nitrogen by virtue of a high-pressure pulse microcapsule moulding instrument after chitosan and gelatine B are mixed, carrying out secondary freeze-drying after microspheres subjected to primary freeze-drying are crosslinked and cured with saturated tripolyphosphate-85% ethanol solution, fully hydrating the microspheres obtained by the secondary freeze-drying, adding cross linking agent carbodiimide / N-hydroxy succinyl, modifying the microspheres with gelatine A, removing unreacted carbodiimide / N-hydroxy succinyl and gelatine A by washing after water bath and reaction in dark place are carried out, and carrying out tertiary freeze-drying, thus the three-dimensional porous chitosan / gelatine microspheres with the diameter of 300-800Mum and the surface aperture of 50-200mu m are obtained, and the surface of each microsphere is connected with the aperture of the interior of the microsphere. The three-dimensional porous chitosan / gelatine microspheres obtained by the invention can be used for in vitro culture of high-density and high-activity liver cells.

A preparation method of a multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement takes plant oil as continuous phase, disperses the aqueous solution of gelatin in the plant oil to form emulsion and obtains the gelatin microspheres of different grain diameters by controlling the temperature and stirring time of the plant oil; corresponding substances is weighed according to the mass rate (5-20): (95-80) of the gelatin microspheres to the calcium phosphate cement powder, compound powders are obtained after uniform mixing and formulates compound powder sizing agent is prepared by blending NaH2PO4 with the mass percent concentration being 0.8 percent with the compound powder under room temperature for 60-90s; and the blended sizing agent is filled into a mold to be solidified for 1-2h under the conditions of constant temperature of 37 DEG C and 100 percent humidity and finally the compound carrier is obtained. The gelatin microspheres and the bone cement are compounded to construct the drug release compound carrier and encapsulated drug types are designed according to the difference of degradation rates of the gelatin microspheres, so as to realize the timed and quantitative release of different drugs, and simultaneously, as a bone repair support, the drug release carrier can finally substitute a calcium phosphate cement carrier support.

The invention relates to an antibacterial biological activity stent and a preparation method thereof. The method comprises the steps that gelatin microspheres are prepared; porous gelatin microspheres are prepared; gelatin microspheres carried with active factors are obtained; an antibacterial stent is prepared, wherein the preparation of the antibacterial stent comprises the steps that at least one of collagen, chitosan and silk fibroin and nano-silver grains are added to acetic acid or malonic acid to prepare a mixed solution, the mixed solution is poured into a die which is horizontally paved with the solid gelatin microspheres, the die is rapidly placed in an ultra cold refrigerator for freezing treatment, and the die is frozen and dried to obtain the antibacterial stent; and the antibacterial biological activity stent is prepared by the steps that the gelatin microspheres carried with the active factors are placed into normal saline to prepare a suspension liquid, and the suspension liquid is poured into the antibacterial stent to obtain the antibacterial biological activity stent after dried at the room temperature. According to the antibacterial biological activity stent and the preparation method thereof, pure natural polymers are taken as raw materials, the nano-silver grains and the bioactive factors are compounded to prepare the stent, so that the stent has the biological activity and antibacterial capacity, can effectively promote vascularization of cambium and can be used for skin injury healing.

The invention belongs to the technical field of functional macromolecular materials and organic and inorganic composite materials, particularly relating to porous magnetic gelatin microspheres and the preparation method. The porous magnetic gelatin microspheres are produced as follows: the gelatin-coated magnetic gelatin microspheres of magnetic nano-particles are obtained through emulsification; then in the catalysis of ammonia, tetraethyl orthosilicate is hydrolyzed and condensed to form loose porous silicon dioxide on the internal and external surfaces of the magnetic gelatin microspheres so as to obtain the porous magnetic gelatin microspheres. The size of the microspheres is of micron degree. The method of the invention is simple to operate and highly efficient; the prepared microspheres are of uniform size; besides, the size of the microspheres and the coating thickness of the silicon dioxide can be adjusted based on reaction conditions. The microspheres have high thermal stability and mechanical stability, as well as high kernel type selectivity of magnetic particles. The microspheres have unique loose porous structures and can be used as fine carriers of drugs and enzymes and can also be used as vascular embolization materials and sewage treatment agents, with a very good application prospect.

The invention belongs to the field of the preparation of gelatin derivative materials and in particular relates to a composite porous microsphere material formed by compounding gelatin, calcium phosphate and an antler polypeptide bioactive tissue or bone morphogenic protein (BMP). The composite porous microsphere is formed by uniformly dispersing, in a gelatin composite porous microsphere, the calcium phosphate and active-substance antler polypeptide or bone morphogenic protein which is capable of promoting bone growth; wherein, the weight of the active-substance antler polypeptide or the bone morphogenic protein accounts for millesimal to five percent of the weight of the gelatin; soluble calcium salt which forms the calcium phosphate ranges from 0.0003 to 0.03 mol in each gelatin; and the amount of soluble phosphate causes the ratio between Ca element and P element to range from 1:1 to 2:1. The composite porous microsphere material can also contain crosslinking agent. The composite porous microsphere material is a bone repair biological material which has good biocompatibility and good bone induction activity.

The invention relates to a method for preparing a gelatin microballoon embolization agent, which comprises the following steps of dissolving gelatin or mixture of gelatin and medicine at 30-70 DEG C, filtering to obtain gelatin solution with 15%-60% of solid content, adding gelatin solution into oil phase liquid paraffin with mass ratio of 1%-2% of stabilizer Span 80, wherein the volume ratio of water and oil is 1 / 1-1 / 10, stirring for 15 minutes by 200-800r / min, adding cross-linking agent aldehyde compound with mass percent of 2%-50% at low temperature of 0-10 DEG C, solidifying for 1-2 hours, washing or drying the cross-linking agent to obtain the microballoon embolization agent after freeze drying or dehydration. The method has the beneficial effects that the gelatin microballoon embolization agent has certain elasticity and expansibility, the surface is smooth, the sphere is obvious, the size and the shape of the sphere is uniform, the grain size is controllable, clinical embolization effect can be improved, and side effect can be lowered, the clinical controllability is strong, antitumor drug can be added into the microballoon in manufacture process, so that double therapeutical effect of medicine treatment and embolization can be realized. The gelatin has good biocompatibility and biodegradablity, and clinical safety can be guaranteed.

The present invention relates to an enrofloxacin gelatin microsphere and its preparation method. It is characterized by that it uses type A gelatin as carrier, uses enrofloxacin as medicine to be carried and adopts an emulsification-condensation process to prepare the invented gelatin microsphere which can take the lung as target organ after intravenous injection. The average grain size of said microspheres is 12 micrometers.

The invention discloses a chitosan / silk fibroin stent with a loaded curcumin / gelatin microsphere compound, and a preparation method and applications thereof. The preparation method comprises followingsteps: mixing a silk fibroin solution with a chitosan solution according to a volume ratio of 1:1 to obtain a chitosan / silk fibroin composite solution; dropwise adding a curcumin solution with a concentration of 10-50 mg / mL into gelatin microspheres, placing the system in an environment with a temperature of 4-25 DEG C overnight, then freeze-drying the solution to obtain a curcumin / gelatin microsphere compound; then adding the curcumin / gelatin microsphere compound into the chitosan / silk fibroin composite solution, evenly stirring, and then freeze-drying the solution to obtain the chitosan / silk fibroin stent with a loaded curcumin / gelatin microsphere compound. The porosity of the stent is 75 to 95%; the aperture is 50 to 200 [mu]m, and moreover, the stent has a long-term antibacterial function and a drug sustained-release function, and can be used to prepare skin wound dressing.

The invention relates to a construction method of a medical titanium alloy implant surface growth factor delivery system, which comprises the following steps that: micro-groove ridges are prepared on the surface of a medical low-elastic Beta titanium alloy; gelatin is dissolved into deionized water, and then 2-imine hydrogen chloride mercaptan is added to be dialyzed and dried, to prepare SH gelatin; the SH gelatin is dissolved in double-distilled water to be ultrasonically homogenized to prepare B liquid, emulsifier is added into liquid paraffin, and then the B liquid is dripped into the liquid paraffin which is added with the emulsifier, isopropyl alcohol and ether are cleaned, added with glutaraldehyde and cured, ether elutes, dries and sieves out the cured substance, and 60Co irradiates and sterilizes, to prepare SH gelatin microspheres; and the microspheres are soaked into rhBMP2 guanidine hydrochloride saturated solution and freeze dried under a vacuum condition, to prepare the SH gelatin microspheres loaded with growth factors. Medical low-elastic Beta titanium alloy implant material is soaked into dopamine solution, and a layer of adhered polymer thin film is generated on the surface of the titanium alloy; and the implant material which decorates the surface is put into the growth factor SH gelatin microsphere solution, vacuum is pumped, and the medical titanium alloy implant surface growth factor delivery system is prepared.

The invention relates to medicinal gelatin microspheres and a preparation method thereof, and belongs to the field of medicine preparation. The active ingredient of the medicinal gelatin microspheres is trimetazidine or a medicinal salt of the trimetazidine. The preparation of the microspheres adopts a chemical cross-linking method, and comprises steps of emulsification, deoiling and dewatering, chemical cross-linking, washing and drying, and the like. According to the preparation method, the particle size, the appearance, the encapsulation efficiency, the drug loading rate are adopted as indexes to comprehensively optimize the carrier, the oil phase, the dewatering and deoiling agent, the cross-linking curing agent, and other reagents added in the process, wherein the carrier, the oil phase, the dewatering and deoiling agent, the cross-linking curing agent, and other reagents are suitable for preparation of the microspheres of the present invention. The prepared microspheres have characteristics of smooth surface, particle size of 50-200 mum, drug loading rate of 30-35%, and has a slow release effect under the common dissolution conditions. In addition, the blood drug level of the microspheres is stable in vivo, and is better than the blood drug level of ordinary tablets.

The invention discloses a preparation technology of a novel nerve conduit, and belongs to the technical field of tents of biological tissue engineering. The preparation technology sequentially comprises a preparation technology of GDNF (glial-derived neurotrophic factor)-carried gelatin microspheres, a synthesizing technology of gelatin-methacrylamide hydrogel, a preparation method of polycaprolactone / gelatin electricity texture nerve conduits, and a combining method of the electricity texture nerve conduits and the GDNF-carried gelatin microspheres. The preparation technology has the advantages that a tent structure with good degradability, biological compatibility and biological safety is realized, and the GDNF is controlled and released by the GDNF-carried gelatin microspheres; compared with blank groups and collagen conduit groups, the regeneration of peripheral nerves is effectively promoted, and the structure and function of restoring damaged nerves can reach the effect similar to the effect of autotransplantation groups; the defect nerves can be bridged, and the stability of axon regeneration environment can be maintained; the invasion of peripheral connective tissues is prevented; the saturing of near and far-heart breaking ends of smaller nerve defect is solved, and the restoration of larger nerve defect is solved.

The invention relates to a smooth-surfaced gelatin microsphere and a preparation method. The preparation method comprises the following preparation steps of: under stirring, uniformly mixing a gelatin aqueous solution with liquid olefin at the temperature of 37-70 DEG C, emulsifying for 5-50 minutes, then mixing the mixed liquid with the liquid olefin at the temperature of minus10-0 DEG C, mechanically stirring, adding 75% (minus18-0 DEG C) of ethanol, stirring, settling for more than 10 minutes, and washing the gelatin microsphere three times through the ethanol (50-100% of ethanol) with gradient concentration to further dehydrate and wash out the liquid olefin remained on the surface of the microsphere; and finally carrying out air drying for a period of time to obtain the gelatin microsphere. The invention has the advantages that the gradient ethanol is used for dehydration after emulsification, and the smooth-surfaced gelatin microsphere is obtained under the condition of not using a surfactant. The invention has simple preparation process, no need of an organic solvent and fewer consumed oil phases, the smooth-surfaced gelatin microsphere can be obtained as long as the gradient ethanol is used for dehydration and other preparation parameters are changed, and a simple and easy method is supplied for efficiently preparing the smooth-surfaced gelatin microsphere.

The invention discloses multi-core adhesive microspheres capable of loading a water soluble low molecular medicament. The microspheres are ionic cross-linked chitosan / gelatin microspheres, wherein acrylic resin microspheres for wrapping and controlling the releasing of the water soluble low molecular medicament are dispersed in the microspheres. A preparation method comprises the following steps of: dispersing or dissolving the medicament into solution of acrylic resin-containing organic solvent; preparing medicament-loading microsphere cores by an emulsification solvent volatilization method; dispersing the cores in the solution of chitosan and gelatin; preparing W / O type mixture from the mixture and oily ingredients; solidifying the gelatin at a low temperature and separating the microspheres from the oil phase; and finally performing cross linking with ionic cross-linking aqueous solution. The chitosan medicament-loading microspheres have the advantages of controllable particle size distribution, good release control effect of water soluble medicament, adjustable releasing rate, high biological adhesion, simple preparation method, and mild condition.

The invention discloses a gelatin sustained release microsphere loaded with growth factors and a preparation method thereof. The gelatin sustained release microsphere loaded with the growth factors and the preparation method thereof take blood platelet compound growth factors as a packaging object, take gelatin microspheres as a carrier, and is prepared by freeze drying after infiltration and expansion. The prepared gelatin sustained release microsphere loaded with the growth factors can effectively use the compound growth factors, can achieve the effects of partially slow release and long lasting effects, can maintain everlasting biological activity, can promote the tissue regeneration and wound healing better, and can provide reference for the preliminary development of the gelatin sustained release microsphere lyophilized product loaded with the blood platelet compound growth factors. The preparation method provided by the invention is simple and is convenient to operate, the whole reaction process is mild in condition and the market prospect is broad.

The invention discloses a carrageenan and gelatin microsphere embolization agent and a preparation method thereof. The carrageenan and gelatin microsphere embolization agent comprises mixed glue formed by carrageenan and gelatin, a biodegradable polymer, an emulsifier and a crosslinking agent. The carrageenan and gelatin microsphere embolization agent is a novel microsphere integrated with embolism and medicine administration functions, and can be applied to treatment of diseases such as tumors and the like. By adopting the preparation method of the microsphere, the novel microsphere, which has the advantages of wide grain diameter distribution range, large drug loading capacity, long embolization time, controllable in-vivo degradation time and the like can be prepared. The function of the existing microsphere is perfected, the carrageenan and gelatin microsphere embolization agent is prepared by using an emulsion crosslinking method, the carrageenan and gelatin microsphere embolization agent is simple in process, and convenient to operate, and good news is brought about for a patient.

The invention discloses a gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold and a preparation method, belonging to the technical field of high molecular materials. The preparation method comprises the following steps: dropwise adding gentamicin sulfate solution with concentration of 10-50 mg / ml into gelatin microspheres, placing overnight at the temperature of 4-25 DEG C and then performing freeze drying to obtain a gentamicin sulfate / gelatin microsphere complex; adding the gentamicin sulfate / gelatin microsphere complex into 2-10 percent by weight of silk fibroin solution, uniformly stirring and then performing freeze drying to obtain the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin porous composite scaffold, wherein the porosity of the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold is 75-95 percent, the aperture of the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold is 50-200 mum, and the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold has long-acting antibacterial and drug slow-release functions and can be applied to the fields of skin wound healing and wound dressing.

The invention relates to a preparing method for a gelatin microsphere of fixed alcohol dehydrogenase by micro-porous membrane permeation and emulsification. The method comprises the following steps that solid gelatin and alcohol dehydrogenase are dissolved in deionized water, and a gelatin / alcohol dehydrogenase solution called as a water phase is prepared; oil soluble emulsifier span-80 is added into liquid paraffin, and liquid paraffin oil called as an oil phase is prepared; a micro-porous membrane is immersed into the liquid paraffin oil, according to the volume ratio of the gelatin / alcohol dehydrogenase solution and the liquid paraffin oil, the gelatin / alcohol dehydrogenase solution is pressed to penetrate through the micro-porous membrane and enter into the liquid paraffin oil, and W-shaped or O-shaped emulsion is formed by the emulsification; glutaraldehyde is added into the W-shaped or O-shaped emulsion, and after cross linking, solidifying, washing and drying are carried out, the gelatin microsphere of the fixed alcohol dehydrogenase is obtained. The preparing method for the gelatin microsphere of the fixed alcohol dehydrogenase by the micro-porous membrane permeation and emulsification has the advantages that the preparing condition is warm, the preparing technology is simple and easy to carry out, the granularity of the prepared microsphere is even, the size is controllable, the storing stability is good, the leakage rate of the alcohol dehydrogenase in the using process is low, and the anti-acid-base environment capacity is strong.

The invention discloses a gel-poly(lactide-co-glycolide) (PLGA) two-phase gradient transition cartilage-bone repair material and preparation thereof. The material is cylindrical and consists of an upper material and a lower material, wherein the upper material is glycidyl methacrylate-modified carboxymethyl chitosan and gelatin; the lower material is porous PLGA. The preparation process comprises the following steps: preparing a gelatin microsphere serving as a pore-forming agent into a PLGA porous scaffold of which one end face is a conical concave surface; preparing a gel solution by using the modified chitosan and modified gelatin; dripping the gel solution on the PLGA porous scaffold of the conical concave surface, inverting, performing ultraviolet light cross-linking to obtain the gel-PLGA two-phase gradient transition cartilage-bone repair material. The material has high biocompatibility and biodegradability, is in a two-phase gradient transition structure, is tightly combined without drop and is simple in preparation process.

The invention discloses a sanguinarine / gelatin microspheres-loaded composite hydrogel support and a preparation method and an application thereof. The method comprises the following steps: adding a sanguinarine solution dropwisely into gelatin microspheres, placing the materials over night, performing freeze drying to obtain a sanguinarine / gelatin microspheres compound; then adding the compound in an aldehyde glucan-aminated sodium hyaluronate pre-gel solution, uniformly stirring the materials, standing gel at normal temperature, and performing freeze drying to obtain the product. The water absorption of the composite hydrogel support is 23-25 times, the porosity is 68-88%, the aperture is 50-200 [mu]m, the composite hydrogel support has functions of wide-spectrum anti-microbial, long-acting anti-microbial and slow release of medicine, and can be used in the fields of skin wound healing and wound dressing.

A gelatin-calcium alginate core-shell structure sustained release microsphere loaded with bone morphogenetic protein (BMP) sequentially comprises an inner core layer and an outer wrapping layer from interior to exterior. The inner core layer is mainly composed of gelatin microspheres on which BMP is evenly dispersed, and the outer wrapping layer wrapping the inner core layer is mainly made of calcium alginate. The preparation of the sustained release microsphere comprises two modes. The first mode is that firstly, blank gelatin microspheres are prepared and then mixed with the calcium alginate to prepare a gelatin / sodium alginate blank core-shell structure sustained release microsphere, finally, the BMP is added, and the gelatin-calcium alginate core-shell structure sustained release microsphere loaded with the BMP is obtained. The second mode is that firstly, gelatin microspheres loaded with BMP are prepared and then mixed with sodium alginate to obtain the gelatin-calcium alginate core-shell structure sustained release microsphere loaded with the BMP. The product has the advantages that the sustained release effect is good, the side effect is slight, the cost is low, and no toxic or side effect is caused.

The invention relates to a rutin-arabic-gum slow-release gelatin microspheres and preparation thereof, belonging to an assistant curing medicine of microorganism infection. The preparation method comprises using rutin as material, adding natural polymer materials gum Arabic and gelatin as the base material frame for the preparation of the microsphere; the weight ratio is that rutin: Arabic: gum=1: 3: 3. The rutin-arabic-gum releasing gelatin microspheres prepared in the invention effectively avoids the characteristic of worst solubility of the rutin in the water to obtain the characteristic of fast effect, high bioavailability, little poisonous side effect and convenient prescription and so on.

The invention discloses a double-drug release PMMA compound bone cement and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing a surface porous osteoporosis drug-loading gelatin microsphere; loading an antibiotic drug onto the surface porous osteoporosis drug-loading gelatin microsphere, thereby acquiring a double-drug loaded gelatin microsphere;uniformly mixing the double-drug loaded gelatin microsphere with solid PMMA bone cement powder, thereby acquiring a solid phase of double-drug loaded PMMA compound bone cement; mixing the solid phasepowder of double-drug release PMMA compound bone cement with a PMMA bone cement liquid-phase reagent, thereby acquiring the double-drug release PMMA compound bone cement. In the double-drug release PMMA compound bone cement prepared according to the invention, the gelatin microsphere with double-drug loaded on interior and exterior is added into the solid phase; the staging release of double drugis realized; the double-drug release PMMA compound bone cement is antibacterial and anti-inflammation, has a certain curative effect to osteoporosis, is low-cost, has no special requirement for production equipment and has an excellent application prospect in clinic.

The invention provides a preparation method and application of gelatin microspheres. The preparation method includes steps: (1), dropwise adding a preheated gelatin solution into preheated liquid paraffin containing span 80 while stirring to prepare stable water-in-oil liquid drops; (2), cooling the water-in-oil liquid drops in ice bath to form cured microspheres; (3), adding in a genipin solutionfor crosslinking; (4), adding in isopropanol for demulsification, applying isopropanol and purified water for centrifugal washing, and freeze-drying to finally obtain dried gelatin microspheres. Thegelatin microspheres are high in sphericity and dispersity, low in cytotoxicity, of a netty structure and capable of adsorbing and slowly releasing growth factor and can be used for in-vivo injectiontherapy.

The invention belongs to the veterinary antibiotics preparation field and relates to a novel preparation of tilmicosin and salt of same for livestock and a preparation method thereof. The preparation is of lung targeting gelatin microsphere preparation by using tilmicosin or tilmicosin salt as principal components. Clinical drug experiments show that compared with conventional preparations the lung targeting gelatin microsphere of tilmicosin and tilmicosin salt of the invention has better organization selectivity, the drug concentration of lung tissue is higher, the duration is longer, the drug effect is longer and more effective and the side effect is low.

The invention discloses a preparation method of gelatin microspheres and an application thereof. The preparation process comprises the following steps: S1. a gelatin aqueous solution is used as the internal phase; an oil phase is an external phase, and the flow rates of the inner and outer phases are respectively controlled by a microfluidic device to be 1 to 10 [mu]L / min and 20 to 200 [mu]L / min;and the external phase is the collected phase, and the micro-droplets are collected; and S2. the collected micro-droplets are gelatinized at 4 to 12 DEG C, the oil phase on the surface is removed, anda crosslinking reaction is carried out with a genipin solution for 1 h, and after the reaction is completed, the gelatin microspheres are obtained by separation. The gelatin microsphere of the invention not only has a simple preparation process, is safe to the cells, is non-toxic, and has certain mechanical strength, can load the cells well, does not affect the activity of the cells, and can be applied as a cell carrier; the gelatin microsphere has a certain promoting effect on the healing of diabetic wounds and the formation of wound blood vessels, also has a good degradation effect, and canbe used as an accelerator drug for wound healing and blood vessel formation.

The invention discloses a preparation method of drug-loadable gelatin embolization microspheres. The preparation method comprises the followings steps: dissolving gelatin into injection water to prepare a gelatin water solution; then putting the gelatin water solution into a water bath at the temprature of 40 to 70 DEG C and stirring; carrying out ultrasonic treatment and heat insulation for lateruse; taking an oil-phase solution and putting into a reaction container; adding the gelatin water solution into the oil-phase solution; after dropwise adding the solution, reducing the temperature ofa reaction system; adding an alkaline substance to regulate the pH (Potential of Hydrogen) value of the system; adding a crosslinking agent water solution and controlling the time for dropwise adding; after dropwise adding the solution, continually reacting at constant temperature; after reaction is finished, carrying out post-treatment on an obtained solution to obtain the drug-loadable gelatinembolization microspheres. The gelatin microspheres, which are synthesized through the method in a single batch, have a uniform grain diameter size and a narrow grain diameter range; the reaction process is controllable, the gelatin microspheres with different specifications can be synthesized through controlling reaction conditions and the grain diameter range of the microspheres is 50 to 2500 mum. The drug-loadable gelatin embolization microspheres synthesized by the method have the advantages that the degradation time range is reduced and the embolization efficiency is improved.

The invention discloses a composite scaffold. The composite scaffold is prepared from naringin, gelatin microspheres, hydroxyapatite and silk fibroin. The composite scaffold is prepared by the following steps: 1) dropwise adding a 0.5-1g / ml naringin solution into the gelatin microspheres, then standing at the temperature of 2 to 8 DEG C over night, and performing freeze drying to obtain a naringin / gelatin microsphere compound; 2) mixing the hydroxyapatite and the naringin / gelatin microsphere compound with a 3.5 to 10 percent by weight SF solution, injecting the mixed solution into a pore plate, then performing freeze drying on the sample to obtain an NG / GMs / HA / SF composite scaffold. The traditional SF / HAp composite scaffold is loaded with the traditional Chinese medicinal monomer, and encapsulated with gelatin microspheres conforming to a bone microenvironment, thereby promoting healing of OVF and enhancing the mechanical strength of the fractured vertebral body; the composite scaffoldhas high rheological property, plasticity, biomechanical strength and higher bone formation prompting performance, so that the composite scaffold can be applied to bone defect areas of different shapes, has a durable effect and higher bone tissue repair ability, and is convenient for large-scale popularization and application.