297 results about "Glucosidase activity" patented technology

The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

The invention discloses a method for brewing a blueberry wine. The method comprises the steps of selecting raw materials, selecting strains, fermenting with ethanol, carrying out malo-lactic fermentation, ageing, clarifying, stabilizing and the like. According to the method, optimized blueberries Huangshan 1# serve as the raw materials; high-temperature enzymolysis, high-temperature start-up and low-temperature long-time fermentation are carried out by using saccharomycetes RC212 as the fermentation strains; the malo-lactic fermentation is carried out by using oenococcus oeni SD-2 with high glucosidase activity; micro-aerobic ageing is utilized, so as to accelerate wine ageing and improve the quality of the wine; the blueberry wine can keep a clarified and stable state in long term by the technical means of natural clarification, cellaring, cold treatment, heat treatment, stabilizer adding, microfiltration and the like. In accordance with the method, a high-quality blueberry wine with good color, smell and taste can be obtained, and the obtained product meets the requirements of the national wine product quality standard GB2758-81.

The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.

The invention herein encompasses methods effective to stimulate epithelial cell proliferation and / or enhance epithelial moisturization and lubrication in a mammalian subject utilizing a composition comprising one or more inhibitors of beta -glucosidase activity or beta -glucocerebrosidase activity. The composition of the method may alternatively comprise a glycosphingolipid, particularly glucocerebroside, or a combination of the above inhibitor(s) and a glycosphingolipid. The method is effective to enhance the cosmetic appearance of skin and promote healing of skin and mucous membranes damaged or deficient from aging, traumatic wounds, photo-aging and a variety of atrophic conditions. The method may be applied to cells in culture. Also included in the invention is a composition comprising one or more inhibitors of beta -glucosidase and a glycosphingolipid useful to stimulate cell proliferation and enhance tissue moisturization and lubrication.

The invention relates to separation, selection and identification of lactobacilli having a function of inhibiting activity of alpha-glucosidase, in particular to a lactobacillus plantarum and an application thereof. Lactobacilli are separated out from intestinal canals of longevous people in Bama of Guangxi; on the basis of comprehensive comparison of acid resistance, cholate resistance, in-vitro antioxidant ability and sensitivity to antibiotics, a strain having a best inhibition function to activity of alpha-glucosidase is selected out by means of in-vitro tests; the strain is identified as Lactobacillus plantarum Grx16 by means of physiological and biochemical tests, API reagent strips and 16s rDNA sequencing analysis. The lactobacillus plantarum Grx16 can be used for preparation of fermented milk having the function of inhibiting activity of alpha-glucosidase.

The present invention relates to the treatment of viral infections, particularly HBV and HCV infections, with a combination comprising a vaccine against a virus antigen and compounds that inhibit glucosidase activity in the host cell.

The invention discloses a lactobacillus plantarum (LP-ONLLY) and the application thereof in active fermented milk. The LP-ONLLY is preserved in China General Microbiological Culture Collection Center on December 6, 2004, and the preservation register number is CGMCC NO.1258. The LP-ONLLY has the high aminopeptidase activity, high beta-galactosidase activity and high glucosidase activity, and frozen and dried living bacterium powder is used as an independent leavening agent which can be applied for preparation of the active fermented milk. The active fermented milk contains the high probiotics live bacteria, the live bacteria have the high stability and can supplement probiotics, and the health function of the LP-ONLLY is achieved.

The invention provides a dogberry medicament having alpha-glucosaminidase inhibiting activity, and the water or organic solvent extract of dogberry, wherein the dogberry extract comprises total glycosides and poly phenols constitution.

Asiatic acid and madecassic acid are extracted and prepared from Centella asiatica (L.) Urban, and pharmacological experiments prove that the asiatic acid and the madecassic acid have the activity of inhibiting alpha-glucosidase. The invention discloses a new use of the asiatic acid and the madecassic acid in the preparation of alpha-glucosidase inhibitor drugs, and the asiatic acid, the madecassic acid or a composition containing the asiatic acid and the madecassic acid can be used for preparing the alpha-glucosidase inhibitor drugs. The invention simultaneously discloses a new preparation method of the asiatic acid and the madecassic acid.

The invention discloses a preparation method of extract of effective part of Clinopodium chinense (Benth.) O. Kuntze for preventing and treating diabetes and an application thereof. The extract of the effective part is prepared from Clinopodium chinense (Benth.) O. Kuntze as a raw material, a concentrate is obtained by pulverizing, ethanol extraction and reduced pressure recovery, the concentrate is extracted by petroleum ether and ethyl acetate, and the obtained ethyl acetate extract is purified by macroporous resin. The extract of the effective part mainly comprises total flavone and terpenoids, and has obvious effects of reducing blood sugar of rats with streptozotocin diabetes, reducing the content of serum cholesterol, protecting the islet cells, inhibiting the activity of alpha-glucosidase, and protecting the vascular endothelial cells. Thus, the extract of the effective part of Clinopodium chinense (Benth.) O. Kuntze has favorable functions for reducing blood sugar, reducing blood fat, and protecting the islet cells and the vascular endothelial cells, and can be used for preventing and treating diabetes, dyslipidemia induced by diabetes and diabetic cardiovascular and cerebrovascular complications.

The invention discloses a mulberry active phenolic compound and a pollution-free extraction and purification method thereof. The method comprises the steps that microwave and vapor high temperature instantaneous enzyme deactivation is carried out on fresh mulberries in sequence, then mechanical crushing, centrifugal layering, food grade acidified alcohol extraction, vacuum concentration and ultrafiltration membrane purification are carried out, and therefore a mulberry phenolic compound extracting solution with the biological activity is obtained. An organic reagent with the low concentration is adopted, mulberry crushing and phenolic compound extraction and purification are achieved under the mild condition, and the natural character and biological activity of the mulberry phenolic compound can be maintained. Compared with a conventional extraction method, the mulberry extracting solution has the higher-concentration total phenol and total anthocyanins, the extraction solution has the good color and luster and high oxidation resistance, alpha-glucosidase enzymatic inhibitory activity and food safety, and the mulberry active phenolic compound can be used as a safety raw material of antioxidant and hypoglycemic drug and health care product development. The conditions are mild, operation is convenient, and industrial production of high-activity natural products is facilitated.

The invention belongs to the biotechnical field, especially relates to a pentapeptide with an auxiliary hyperglycemic function, and concretely relates to a pentapeptide KLPGF capable of inhibiting the activity of alpha-glucosidase and inhibiting the activity of alpha-amylase. The amino acid sequence of the pentapeptide KLPGF is Lys-Leu-Pro-Gly-Phe. The pentapeptide can be used to develop auxiliary hpyerglycemic drugs and functional foods.

The invention provides a Cyclocarya paliurus callus and a suspension culture method for callus particles. The method mainly comprises the following steps: 1) selection and aseptic processing of plants, wherein, healthy Cyclocarya paliurus plants at an age of 3 to 7 months and 9 to 11 months are selected, annual twigs and tender leaves are cleaned, immersed in a washing agent for 5 to 20 min and flushed with clear water for 0.5 to 2 h, and under an aseptic condition, cleaned twigs and tender leaves are disinfected with alcohol having a concentration of 70 to 80% for 5 to 30 s and then are sterilized by using mercuric chloride having a concentration of 0.1% for 5 to 20 min or by using sodium hypochlorite having a concentration of 2 to 12% for 5 to 20 min; 2) induction culture of callus; 3) suspension culture of callus particles; and 4) amplification culture of callus. According to the invention, the growth rate of Cyclocarya paliurus callus can be greatly improved, and during a same period of time, when increment of callus on a solid medium reaches 721%, increment of callus obtained through fluid suspension culture reaches 1292%; according to results of experiments on inhibition of activity of alpha-glucosidase, in the process of fluid suspension culture, secondary metabolites are secreted to the exterior of cells, and callus cells and a culture solution both contain an alpha-glucosidase activity inhibitor.

The invention relates to the field of microorganisms, in particular to a bifidobacterium animal and an application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity. The preservation number of the bifidobacterium animal is CGMCC No.17308. The invention also discloses a product composition. The product composition contains thallus substances and / or metabolites of the bifidobacterium animal. The bifidobacterium animal provided by the invention is suitable for specific physiological physique of Chinese people, and has relatively high advantages in activityinhibition of alpha-glucosidase, transport inhibition of glucose, improvement of glucose tolerance level of organisms, relief of insulin resistance and leptin resistance, improvement of the secretionlevel of glucagon-like peptide-1, increase of islet cell size and islet beta cell number, and the like, so that development of diabetes or hyperlipidemia can be better controlled, and particularly weight gain and obesity of organisms can be controlled.

The invention provides a gene of coded beta-glucosaccharase, which is called Unbgl1B and is obtained by constructing Metagenome DNA library of uncultured microorganisms of alkality contaminated soil and by the detecting and screening method of the activity of the beta-glucosaccharase of clone library, so as to be one of the following nucleotide sequences: 1) DNA sequences and partial sequence thereof in sequence 1 of a sequence table; 2) DNA sequences having more than 80% of homoeology compared with the DNA sequences defined by the sequence 1 of the sequence table. The DNA in the sequence 1 of a sequence table is DNA sequences of a pGEM-3Zf(+) part of a cloning vector and DNA of exogenetic uncultured microorganisms cloned on the vector, and the exogenetic DNA segment consists of 838 basic groups; the GC content of the gene is 54.3%. The gene is used for producing the beta-glucosaccharase, so as to dissociate cellobiose into single glucose molecule.

The invention discloses a high-activity composite cellulase and preparation thereof, and an application method for the same in enzymatic saccharification of wood fiber. According to the invention, Eupenicillium javanicum ZN-205 is subjected to shake-flask fermentation so as to produce beta-glucosidase; best enzyme production conditions are that an initial pH value is 6, the concentration of peptone is 0.75%, the concentration of microcrystalline cellulose is 2.5%, the addition amount of tween-80 is 0.05%, culture temperature is 28 DEG C, the volume of liquid filled in a 250 ml triangular flask is 100 ml, a revolving speed of a shaker is 175 r / min, inoculum amount is 5% and the highest activity of beta-glucosidase is 2.312 IU / ml; beta-glucosidase prepared from Eupenicillium javanicum ZN-205 and cellulase prepared from Trichoderma reesei Rut C-30 are compounded, and an obtained optimal ratio of beta-glucosidase activity to filter paper activity of 1.4. Utilization of the compounded enzyme for enzymatic saccharification of wood fiber enables high-efficiency and low-cost enzymatic saccharification effects to be achieved.

The invention provides a method for measuring Beta-D-glucosaccharase activity in propolis. The enzymatic activity is determined by the amount of the Beta-D-glucosaccharase which can hydrolyze a given amount of the substrate into pnitrophenol in a certain time using spectrophotometric determination method. The method can determine the presence of the Beta-D-glucosaccharase in propolis, and distinguish the difference of the propolis from the other propolis plant from the angle of the component and pharmacological activity, and distinguish the true propolis from the false propolis from the angle of the raw material propolis, thereby providing the criterion of the propolis quality standardization. The method has features of simpleness, high sensitivity and low measurement cost.

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

The present invention relates to processes for producing a fermentation product comprising (a) saccharifying starch-containing material below the initial gelatinization temperature in the presence of i) from 0.001-50 AGU / g DS, preferably 0.01 to 10 AGU / g DS alpha-glucosidase activity more than the native amount of endogenous alpha-glucosidase present in the starch-containing material, and ii) from 0 (zero) to 10 FAU-F / g DS of alpha-amylase activity, and (b) fermenting using a fermenting organism. The invention also relates to an enzymatic composition for use in a process of the invention.

The invention discloses a high-temperature-resistant high-yield cellulase bacillus subtilis and application thereof. A cellulase production strain is separated from surface soil of a corn straw pile in a Jiangjin area of Chongqing, and the preservation number of the bacillus subtilis is CCTCC NO: M 2020002; a strain Z2 with the best enzyme production performance is finally obtained through enrichment culture, a CMC-Na plate experiment, a lignin plate experiment and an enzyme production experiment, and the obtained strain is subjected to 16S rRNA sequence comparison and identification to determine that the strain is bacillus subtilis Z2. By optimizing the culture medium and the culture conditions, the filter paper enzyme activity, the CMC enzyme activity and the beta-glucosidase activity under the optimal enzyme production conditions of 0.800 U / ml, 5.20 U / ml and 2.07 U / ml are obtained respectively. Cellulase produced by the strain has relatively high activity and stability under the conditions of a buffer solution with the pH value ranging from 4.0 to 10.0 and the temperature of 30 DEG C and 80 DEG C. Cellulase produced by the strain is mixed with commercial xylanase, then a saccharification experiment is carried out on pretreated straw stalks, and 84.27 mg / ml of total reducing sugar can be obtained.

The present invention provides a method for increasing the activity of a mutant or wild-type a-glucosidase enzyme in vitro and in vivo by contacting the enzyme with a specific pharmacological chaperone which is a derivative of 1- deoxynojirimycin. The invention also provides a method for the treatment of Pompe disease by administration of chaperone small molecule compound which is a derivative of 1-deoxynojirimycin. The 1-deoxynojirimycin derivative is substituted at the N or Cl position. Combination therapy with replacement a-glucosidase gene or enzyme is also provided.

The invention provides a series of labdane type diterpene derivatives and a preparation method and application thereof. The derivatives are compounds containing structures as shown in general formula I, II or III or addition salts formed from compounds and pharmaceutically acceptable acids or alkalis. R1 is hydrogen, hydroxy or C1-C3 alkyloxy or acyloxy; R2 is C1-C3 saturated or unsaturated alkyl, C1-C3 oxygen-containing alkyl or C1-C3 acid and ester thereof or salt. The derivatives employ Labiatae Phlomis plant Phlomis umbrosa as a raw material, and solvent extraction, column chromatography separation and purification are carried out for preparation. According to the invention, based on separation for preparing the labdane type diterpene derivatives, the compounds have activity for inhibiting alpha-glucosidase is verified, so that lead compounds for developing oral medicaments for reducing blood glucose are provided, and the preparation method has important meanings for exploring new purposes of natural products and botanical drugs.

The invention belongs to the field of microorganism, and particularly relates to a microorganism penicillium strain producing high-activity cellulase. The penicillium strain has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M2011195. The invention also provides an application of the strain in cellulose enzymatic hydrolysis. Through mutation breeding, the penicillium strain producing high-activity cellulase is bred; cellulase crude enzyme liquid is obtained through fermentation of the strain, which has filter paper enzyme activity of up to 10 IU / ml, endoglucanase activity of up to 30 IU / ml, exoglucanase activity of up to 1.5 IU / ml and beta-glucosidase activity of up to 8 IU / ml, respectively being 3 times, 4 times, 4.5 times, and 4 times higher than the activities of an original strain; when the strain is applied to enzymatic hydrolysis of cellulose raw materials, the cellulose conversion rate in 3 days is up to more than 80%, and the cellulase has extremely high activity.

The invention relates to lactobacillus plantarum and an application thereof, the lactobacillus plantarum is preserved in China General Microbiological Culture Collection Center on July 22, 2019, and apreservation number is CGMCC No. 18260. The lactobacillus plantarum CGMCC No.18260 is high in growth capacity, high in acid production capacity, high in acid resistance and salt resistance and high in nitrite degradation capacity. In addition, the strain has very strong beta-glucosidase activity, the content of free polyphenol and flavone in the chopped chilies and the pickled vegetables can be greatly increased through inoculated fermentation of the strain, the oxidation resistance of the strain is improved, and meanwhile, the content of nitrite in the chopped chilies and the pickled vegetables is reduced.

The invention relates to a hyperthermophilic glycosidase mutant and an application thereof in preparation of ginsenoside CK, and belongs to the technical field of biotechnology engineering. An amino acid sequence of the hyperthermophilic glycosidase mutant is as shown in SEQ ID NO:2; and the hyperthermophilic glycosidase mutant is applied to preparation of rare ginsenoside CK. The hyperthermophilic glycosidase mutant has high heat resistance and stability; relatively high catalytic activity can still be kept after heat preservation at 70-80 DEG C for over 300 hours, and thus hyperthermophilic glycosidase mutant has the advantages of low storage and transportation cost, and low requirement standards on a cooling system of a reactor when applied to production; the kinetic reaction is accelerated; meanwhile, the hyperthermophilic glycosidase mutant has beta-glucosaccharase activity; glucosides of main protopanaxadiol type saponins C3 site and C20 site can be hydrolyzed on the basis that the saponin structure is not destroyed; and the hyperthermophilic glycosidase mutant is efficient, specific, few in byproducts, high in yield and the like, so that the Fpglula can be applied to industrial production of a rare ginsenoside Compound K.

The invention discloses a high temperature yeast (Saccharomyces cerevisiae) CGMCC No.7513 for producing msalais through fermentation and msalais obtained by a preparation method through fermentation by utilizing the strain. By virtue of analysis at different temperatures, sugar degrees, alcohol contents and low nitrogen tolerances, metabolism property determination, detection of wine production capacity, flocculability, autolysis, H2S production capacity and biogenic amine production characteristic, determination of pectinase activity and determination test on activity of beta-glucosaccharase, the high temperature msalais yeast strain with excellent brewing performance is screened, and the high temperature msalais yeast strain is widely applied to the fields of light industry production and the like.

The invention discloses a lactobacillus plantarum with dual blood glucose-reducing target and an application thereof. The lactobacillus plantarum ST-2 with dual blood glucose-reducing target and higher restraining activity is acquired by screening in the manners of in vitro inhibitory activity, probiotic property determination and principal component analysis; the inhibition ratio of the CFS extract thereof for alpha-glucosaccharase activity is 13.44%; the inhibition ratio for dipeptidyl peptidase-IV activity is 21.525%. An experiment proves that ST-2 has a certain inhibitory activity for differently sourced alpha-glucosaccharase and dipeptidyl peptidase-IV; the carbonylated hemoglobin value of type-II diabetic rat is obviously reduced, so that ST-2 has ultrahigh application value for delaying the carbohydrate absorption of the diabetic after the meal and reducing postprandial blood sugar increasing; ST-2 is prepared into freeze-drying bacteria powder in high viable count; the viable count is as high as 89% of that before freeze-drying; besides, ST-2 has an excellent fermenting effect; the application of ST-2 in producing dairy products or being added as a food ingredient into functional food has excellent preventing and intervening effects for diabetes and obesity.

The present invention relates to a polypeptide associated with the biosynthesis of 1-deoxynojirimycin which is a substance that inhibits a-glucosidase activity, to a polynucleotide coding therefor, to a vector comprising the polynucleotide, to a transformant comprising the vector, and to a method in which the transformant is used in order to produce either a polypeptide associated with the synthesis of 1-deoxynojirimycin or 1-deoxynojirimycin. The polynucleotide of the present invention can be used in the volume production of 1-deoxynojirimycin, or can be used in studies searching for novel 1-deoxynojirimycin derivatives using recombinant technology, and hence is useful in developing pathogenic virus inhibitors and hypoglycaemic agents for diabetic patients.

The invention relates to the technical field of medicines, and discloses glabrous sarcandra herb acidic polysaccharide quality product SGP-2. The glabrous sarcandra herb acidic polysaccharide is characterized in that the content of polysaccharide is over 90 percent, and the number-average molar mass is 1,000-2,000KDa; monosaccharide comprises glucose, galactose, mannose, arabinose and galacturonic acid; the bond types of glucosidic bond comprise alpha(1->4)galacturonic acid, alpha(1->4) galacturonic acid methyl ester, alpha(1->4) arabinose, alpha(1->4)mannose, beta(1->6) glucose, beta(1->3)galactose, alpha(1->4)glucose, beta (1->4,6)glucose, alpha(1->3,6)galactose, and alpha(1->4,6)galacturonic acid. The invention provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in inhibiting alpha-glucosidase activity and application of glabrous sarcandra herb acidic polysaccharide in preparing a medicament for treating diabetes mellitus, and provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in preparing anti-tumor medicaments.