150 results about "Halophilic bacteria" patented technology

A high efficient processing technique for hypersaline waste water generates aerobic granular sludge in a sequencing batch reactor and adopts certain startup method and sluge domestication method, in condition of without halophilic bacteria, sludge in the reactor realize aggregating growth and form composite microecology, so hypersaline waste water can be processed effectively and resistance to salinity shock of the system can be improved.

The invention relates to the field of microorganisms, in particular to an extreme halophilic bacterium, a compound halophilic bacterium agent and application thereof. The extreme halophilic bacteria obtained through screening are respectively salt monad CGMCC NO.3081, pseudomonad CGMCC NO.3082 and bacillus CGMCC NO.3083. The invention also provides the compound halophilic bacterium agent containing the extreme halophilic bacterium. The invention obtains three extreme halophilic bacterium strains with the salt tolerance of 15-25 percent through separation, a compound extreme halophilic bacterium agent formed by compounding the three extreme halophilic bacterium strains can effectively process high salt cyanuric chloride production waste water, and compared with other processing technologies, the method better conforms to the state policies of circular economy and energy saving and emission reduction, can save a large number of running cost and realize the resource processing of waste water.

The invention provides a method for the screening and remediation of a petroleum-contaminated soil bioremediation agent, which specifically comprises the following six steps of: collecting a petroleum degradation autochtonous bacterium source and a halophilic bacterium source, screening petroleum degradation autochtonous bacteria, screening halophilic bacteria, screening the petroleum degradation halophilic bacteria, screening a high-efficiency agent for the remediation of petroleum-contaminated soil and selecting optimal conditions for the remediation of the high-efficiency degradation agent by utilizing an orthogonal remediation experiment. The bioremediation agent screened by the screening and remediation method can be applied to the bioremediation of the petroleum-contaminated soil with the salt content of 10 to 20 g / Kg. The method for the screening and remediation of the petroleum-contaminated soil bioremediation agent has high practical value, and can be widely applied in the fields of bioremediation of the petroleum-contaminated soil, beaches, petroleum production wastewater and the like.

The invention discloses a preparation of efficient phenol-degrading halophilic bacteria and an application thereof, belonging to the technical field of treatment of waste water and sewage. In the invention, halophilic bacteria in the sewage or sludge are taken as a bacteria source; a gradient domestication method is adopted to screen efficient phenol-degrading halophilic bacteria which have strong degradation capability, can adapt to higher salt concentration and can grow well by taking phenol as the sole carbon source in the phenol-containing waste water with the salt content of 5-6% and thephenol concentration of 100-500mg / L; under the environment of high salt and high phenol, the phenol-degrading performance of the mycelium has obvious advantages over that of the traditional activatedsludge method. The strain can be applied to phenol-degrading treatment of waste water to determine the optimal water power retention time and the optimal bacteria input amount and batch processing time, and the operational conditions can be optimized through orthogonal experiments. The screening of the efficient phenol-degrading halophilic bacteria has high application value, and can provide a reference for the removal of phenol in the oil extraction waste water and the industrial treatment of the phenol-containing wastewater with high content of salt in petrochemistry industry.

The invention discloses a fast construction method of a high-salt wastewater biological treatment system. The method is characterized in that activated sludge of a urban sewage treatment plant is inoculated in a sequencing batch type biological reactor to form a biological film on a combination padding, a certain start running method and a sludge acclimation method are adopted to realize apposition growth of the activated sludge in a high-salt environment with the salt content of 3 percent (calculating NaCl, the same below) in the reactor without adding halophilic bacteria, and a complex microbial biological film treatment system suitable for microbe to growth with the definite salt content is formed at last.

The invention discloses a soil conditioner for treating saline-alkali soil, and belongs to the technical field of agricultural production. The soil conditioner is prepared by diatomite, attapulgite, halophilic bacteria, humic acid, fulvic acid and biological floras, 300 kilograms of water is added into fungi in the biological floras to prepare an aqueous solution, and the diatomite, the attapulgite and the halophilic bacteria are added into the aqueous solution; then the humic acid and the fulvic acid are mixed to be added into the aqueous solution to be mixed, and therefore particles are obtained. In tillage, the soil conditioner is applied along with bottom fertilization in soil turning, and can promote rapid growth of crops and increase the yield and incomes, and the quality of fruits is remarkably improved.

The present invention discloses a special compound fertilizer for saline-alkali soil and a production method thereof. The compound fertilizer comprises microbial agents, mineral fertilizers, organic fertilizers, slow-release fertilizers, and controlled-release fertilizers. The microbial agents include halophilic bacteria, alkalophilic bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium bacteria, Bacillus subtilis, Bacillus licheniformis and E. coli; the mineral fertilizers include Chinese medical stone, calcite and zeolite; the organic fertilizers include one or more of cow manure, horse manure, chicken manure, humic acid, milk vetch, alfalfa, crushed straw, leaf mold, soybean meal, peanut meal, fermentation product residues; the slow-release fertilizers include calcium superphosphate and urea formaldehyde; and the controlled-release fertilizers include ammonium sulfate, potassium chloride, potassium sulfate and ammonium chloride coated by semi-permeable membranes with different thicknesses. The fertilizer can effectively improve soil structure, reduce salinity and pH value of the soil, increase soil fertility, and maintain lasting fertility, and is particularly conducive to improving the saline soil.

The invention discloses a construction method of a moderate halophilic bacteria consortium capable of efficiently degrading methylbenzene under a high-salinity condition and growing by using the methylbenzene as only carbon and energy sources. A group of moderate halophilic bacteria consortiums capable of degrading high-salinity benzene series is obtained through screening. The construction method comprises the following steps of: 1) inoculating a high-salinity soil sample polluted by the benzene series in a basic inorganic salt culture medium with NaCl concentration being 1M-3.5M, adding the methylbenzene as the only carbon and energy sources, and conducting aerobic culture under conditions of 30DEG C and 120r / min to obtain bacteria solution; and 2) continuously domesticating the bacteria consortium. The moderate halophilic bacteria consortium obtained by adopting the construction method can adapt to a wide salinity range and can degrade high-concentration methylbenzene by using the methylbenzene as the only carbon source. The moderate halophilic bacteria consortium not only can degrade the methylbenzene at very high speed, but also has a very strong capacity of tolerating high salinity and high methylbenzene concentration. Therefore, the moderate halophilic bacteria consortium has important research advantages and wide application prospect in the field of biological treatment of high-salinity benzene series wastewater.

The invention discloses a production method of a fresh-keeping sausage casing. The production method of the fresh-keeping sausage casing is characterized in that in the sausage pickling working procedure, a sausage casing preservative which is 2-10% of the mass of the sausage casing in sausage coating salt is added, and then pickling is carried. The sausage casting preservative provided by the invention can effectively inhibit the growth and breeding of sausage casing halophilic bacteria; moreover, natural extractives are stable in nature, are safe to use, and guarantee the quality of the sausage casing well; three kinds of fresh-keeping reagents with anticorrosion and bacterial inhibition effects are adopted; meanwhile, the breeding of the sausage casing halophilic bacteria is inhibited effectively by utilizing the synergistic effect of the preservatives, thereby preventing the red sausage coating salt phenomenon and protecting the color of the sausage casing; a biological fresh-keeping agent is low in cost, is less in using quantity, and is convenient to prepare; the red stain phenomenon in the sausage storage and transportation process is reduced; the quality of the sausage casing is protected; the economical loss is reduced; the production efficiency is increased; the biological fresh-keeping agent of the sausage casing is added in the stage of charging salt for sausage casing and picking so as to effectively inhibit the breeding of the halophilic bacteria; the process is simple, and the production cost is low.

The invention discloses a halophilic bacteria fungicide as well as preparation thereof, a biological treatment system containing the fungicide and an application of the fungicide in treating triazine wastewater. The halophilic bacteria fungicide is prepared by separating and screening degrading bacteria of four triazine compounds, and preparing according to a certain proportion, wherein the degrading bacteria include (Salinicoccus roseus )BJ-1, (Brevibacterium iodinum)BJ-2, Bacillusnitritophilus BJ-3, (Halomonasalkaliphila)BJ-4. The fungicide disclosed by the invention has stronger ability of degradation on the triazine wastewater and can be adaptive to a high-salt environment, has a very good degradation effect under conditions with salt content of 1%-15% and atrazine concentration less than 500mg / l, has obvious advantages in performances of degrading the triazine compounds compared with the traditional active sludge method, and has a degradation effect of over 98% for triazine compounds in the wastewater.

The invention relates to a biological preservative and particularly relates to a sausage casing biological preservative and application thereof. The sausage casing biological preservative comprises chitosan, polylysine and nisin, wherein the weight ratio of chitosan to polylysine tonisin is 2: 5: 3. The application of the sausage casing biological preservative is as follows: the sausage casing biological preservative which is constituted by chitosan, polylysine and nisin according to the weight ratio of 2: 5: 3 is added into sausage-casing salt during the stage of salting and curing a sausagecasing, and the using amount of the sausage casing biological preservative is 0.04-0.06%, preferably 0.05% of the mass of the sausage casing. The sausage casing biological preservative can effectively inhibit growth and reproduction of halophilic bacteria of the sausage casing; and furthermore, natural extracts are stable in characters and safe to use, and can better ensure the quality of the sausage casing. According to the sausage casing biological preservative provided by the invention, the sausage casing biological preservative only needs to be added during the stage of salting and curingthe sausage casing, and then the reproduction of the halophilic bacteria can be effectively inhibited, so that process is simple and production cost is low.

The invention discloses Halomonas sp. capable of degrading phenols and a high-throughput screening method and application thereof. The Halomonas sp. PH4-5 is preserved under CGMCC 10666. A bacterial liquid is cultured with a 48-pore deep-pore board, phenol concentration is measured by 96-pore microplate reader plate spectrophotometry based on 4-amino antipyrine colorimetry, moderately halophilic bacteria efficient in degrading phenols are screened from 43 environmental samples contaminated by high salinity and organics, the Halomonas sp. is separated and verified from an optimal flora E3, and a salt tolerant degrading mechanism of the Halomonas sp. is studied. Studying results show that quick screening of environmental degrading bacteria provides methodological basis and also provides technical support for the treatment of high-salinity phenolic wastewater.

The invention provides a method for treating high-salt wastewater by using halophilic microorganisms. The halophilic microorganisms can adapt to a high-salt environment to perform normal growth metabolism, and comprise haloduric and halophilic bacteria, yeasts, archaea and microfauna. The method for treating the high-salt wastewater by using the halophilic microorganisms comprises the following steps: adding a halophilic microbial flora into a biochemical system; performing mixed culture on the halophilic microbial flora in an influent salt concentration range of 1-25% to improve the pollution degradation capability of the halophilic microbial flora and obtain a halophilic microbial aggregate with good mud-water separation performance and precipitation performance, such as active sludge and a biomembrane; and stabilizing the process parameters and influent water quality of the biochemical system to ensure that the biochemical effluent reaches a standard or meets design requirements. By adopting the method provided by the invention, the high-salt wastewater with the influent salt concentration of 1-25% of the biochemical system can be subjected to biochemical treatment, and the halophilic microbial agent only needs to be added once, so that the investment and operation costs can be reduced.

The invention discloses an organic fertilizer fermentation inoculant and application. The inoculant is composed of a primary fermentation inoculant body and a secondary fermentation inoculant body. The primary fermentation inoculant body comprises bacillus subtilis, bacillus licheniformis, trichoderma viride, aspergillus oryzae, pseudomonas, trichoderma koningii, geotrichum candidum, long spiny shells, ascomycetes, thermomyces lanuginosus and thermobifida fusca. The secondary fermentation inoculant body comprises halophilic bacteria, alkalophilic bacteria, azotobacter chroococcum, bacillus megatherium, streptomyces griseus, bacillus thuringiensis, brevibacillus laterosporus and bacillus mucilaginosus. Time for fermenting organic fertilizer with cow dung as the raw material can be effectively shortened, and diseases and insect pests in the fertilizer can be eliminated. Meanwhile, alkalophilic bacteria and bacillus thuringiensis are added so that the fermentation inoculant can adapt to saline-alkali soil and can prevent diseases and insect pests of plants, and the prepared organic fertilizer can improve the soil structure, reduces the salt content and the pH value in soil, improves soil fertility and is particularly beneficial to improvement of saline-alkali soil.

The invention discloses a composite microbial agent, a preparation method thereof and a salty sewage treatment method. The composite microbial agent comprises halophilic archaea, halophilic bacteria and halotolerant bacteria. The preparation method comprises the following steps of performing liquid culturing on each component under different salinities respectively, and mixing bacterium solutions to obtain the composite microbial agent when each bacterium solution contains a certain amount of bacteria. The composite microbial agent can be added into salty sewage to treat the salty sewage. Different strains in the composite microbial agent are in different growth states under different salty conditions, and advantages in the growth of the strains within high, medium and low major salty ranges are achieved, so that the microbial agent can be used for treating organic sewage with different salinities, and is wide in application range.

The invention discloses a compound halophilous-microorganism bacterium agent and an application thereof. Halophilous microorganisms are the microorganisms which are not inhibited or poisoned by high-concentration inorganic salts in the high-salt environment, can carry out normal growth and metabolism and comprise salt-resistant microorganisms and halophilic microorganisms. The compound halophilous-microorganism bacterium agent at least comprises two of halophilic bacteria, halophilic archaea, salt-resistant bacteria, salt-resistant archaea, halophilic fungi and salt-resistant fungi, wherein the types and the quantity of the halophilous microorganisms are at least 10, and the halophilous microorganisms at least comprise one type of uncultured microorganisms. The compound halophilous-microorganism bacterium agent disclosed by the invention has the advantages that high-efficiency and stable degradation can be carried out on organic matters under the condition of 1%-20% salt; by using the compound halophilous-microorganism bacterium agent for biochemical treatment of high-salt waste water and microorganism restoration of saline-alkaline soil, the pollution treatment cost can be reduced.

The invention relates to a method for producing PHA by continuous treatment of molasses alcohol wastewater via mixed halophilic bacteria and belongs to the technical field of production of PHA. The method comprises the following steps of carrying out activation culture on mixed halophilic sludge to obtain halophilic activated sludge, carrying out enrichment culture on the molasses alcohol wastewater inside an SBR reactor by virtue of mixed halophilic bacteria colony, carrying out continuous PHA production on molasses wastewater inside a continuous flow reactor and recovering PHA in the discharged residual sludge. The PHA is synthesized by virtue of the mixed bacteria colony instead of a pure bacteria colony, strict sterilization procedures is not required, the process is simplified and the cost is saved; the continuous flow process is simple, compared with the fermentation process, the repeated replacement of the fermentation broth is not required, the repeated inoculation of bacterial sludge is not required, the continuous water inlet and continuous water outlet are achieved, respective process parameters tend to a constant value and is simply implemented, the automatic control is facilitated, the method is easily operated in engineering, the yield of PHA is high, the continuous production of PHA is achieved and the resource utilization of residual sludge is also achieved.

A 3-hydroxypropionic acid(3HP) degradation way of halophilic bacteria is analyzed for the first time by a transcriptomics method, the inventor finds that the 3-hydroxypropionic acid can degrade genesdddA and dddC, and recombination halophilic bacteria TD delta dddA which cannot degrade 3HP is obtained. Based on this, an alcohol dehydrogenase gene AdhP and an aldehyde dehydrogenase gene AldDTD, which are from the halophilic bacteria endogenous and responsible for production and metabolism of the 3HP are further utilized, and a synthetizing biology means is taken for adjusting and controlling the expression level of the metabolic way. Through screening, the recombination halophilic bacteria high in yield of the 3HP is obtained, and the yield and the productivity are respectively 0.93g / g ofthe 1,3-propylene glycol(PDO) and 2.4g / L / h, which are the highest 3HP yield and productivity reported so far.

The invention discloses a halobacillus and a method for industrially producing Ectoin by using the halobacillus. According to the invention, the method comprises the following steps: firstly, separating a bacterial strain with the Ectoin-secretion characteristic from coastal mud of Rizhao, and then carrying out mutation screening to obtain the Halobacillu sp. FL-2423 with high Ectoin yield and stable hereditary characteristics. The preservation number of the bacterial strain is CGMCC No.18783. The above bacterial strain is moderate halophilic bacteria and is subjected to fermentation culture in a culture medium with a NaCl concentration of 50-100g / L, Ectoin is synthesized in cells, and the fermentation yield reaches 10-16g / L.

The invention discloses a desert area saline-alkali soil amelioration modifying agent and a preparation scheme. The desert area saline-alkali soil amelioration modifying agent is prepared from raw materials in parts by mass: 5 to 18 parts of pH conditioning agent, 15 to 36 parts of inorganic fertilizer, 28 to 45 parts of organic fertilizer, 2 to 4 parts of halophilic bacteria, 4 to 6 parts of rhizopus, 1 to 3 parts of humic acid, 3 to 5 parts of actinomycetes, 2 to 4 parts of rhizobium, 3 to 7 parts of yeast, 0.01 to 0.04 part of auximone and 1 to 2 parts of deodorant. The desert area saline-alkali soil amelioration modifying agent disclosed by the invention can obviously improve the germination rate and the survival rate of plants, reduces a pH value and salt content of saline-alkali soil, improves organic content of the soil and enables the soil to be more suitable for plant growth.

The invention relates to a promoter library and a method for constructing an expression system of the promoter library with different expression strengths in bacteria by using the promoter library. The promoter library comprises a promoter sequence as shown in SEQ ID NO:2 or SEQ ID NO:3, wherein n represents any one of a, t, c and g. The promoter library provided by the invention can be used in various bacteria such as escherichia coli and halophilic bacteria, has consistent expression strength between different bacteria, can be used as a basic genetic element, is used for fine regulation of genetic expression and metabolic pathways and has broad application prospects.

The invention discloses a cultivation method for halophilic bacteria capable of efficiently degrading high-salinity wastewater phenol and an application method of the halophilic bacteria and belongs to the technical field of sewage treatment. The halophilic bacteria in water of a great salt lake are selected by using a beef extract peptone culture medium of which the salinity is 15 to 18 percent, are cultivated by using a phenol liquid culture medium, and are acclimatized through a sequencing batch reactor (SBR), and then the halophilic bacteria are prepared. The halophilic bacteria are low in cost and high in environment adaptability. The problem that medium halophilic bacteria are time-consuming in removing the high-salinity wastewater phenol is solved. The halophilic bacteria have obvious economic benefit, social benefit and wide application prospect.

The invention belongs to the field of biochemical treatment of industrial wastewater and in particular relates to a preparation method of a domestication agent for active sludge in salt-containing wastewater treatment. According to the preparation method provided by the invention, efficient halophilic bacteria is screened from salted meat; a halophilic strain is added to improve the composition ofmicroorganisms, so that the activity of a reaction main body active sludge is enhanced; the strain has a special salt-resisting mechanism, so that the strain is suitable for growing in a high-salt environment and degrading organic matters; the halophilic bacteria can be used for directly absorbing betaine, tetrahydropyrimidine and the like from the environment and also can be automatically synthesized in cells by absorbing substrates; the microorganisms can resist changes of osmotic pressure in the high-salt environment by adopting the mechanism, so that the microorganisms have relatively strong adaptability and the salinity impact resisting capability is improved.

The invention relates to a method for preparing a saline-alkali soil improving agent by utilizing dewatered sludge of a domestic sewage treatment plant and is characterized by effectively utilizing the sludge of the sewage treatment plant, reducing saline and alkali of modified saline-alkali land, dissolving salinity and accelerating the soil desalinization so as to achieve the method for preparing the saline-alkali improving agent meeting the requirements of greening, agricultural production and other planting requirements. The dewatered sludge, cottonseed hull powder, sugar residue mud, xylitol residue and other organic matters are fully utilized, microbial halophilic bacteria and other beneficial florae are added, mixing and fermentation are performed, and sulfur powder, calcium carbonate, ferrous sulfate and other inorganic mineral matters are further added after complete decomposition, thereby preparing the saline-alkali improving agent; and the saline-alkali improving agent can be suitable for improving saline-alkali soil with the ph of less than 11 and the salinity of less than 1.5% and enable the soil to achieve the requirements of agricultural production or a green environment through one to two planting cycles. The proportion of all the raw materials can be regulated according to the saline and alkali content of the local saline-alkali soil, thereby being suitable for the various types of the saline-alkali land.

The invention relates to soil ecological restoration conditioner and a preparation method thereof and a soil ecological restoration method, and belongs to the technical field of soil ecological restoration. The technical scheme includes that the soil ecological restoration conditioner comprises, by weight, 1-5 parts of fly maggot chitin, 1-5 parts of actinomyces, 2-6 parts of bacillus, 1-5 parts of photosynthetic bacteria, 2-6 parts of phosphorous and potassium decomposer, 1-3 parts of halophilic bacteria, 30-50 parts of cow dung, 5-15 parts of zeolite, 5-15 parts of medical stone, and 20-50 parts of slag. The soil ecological restoration conditioner is low in cost in favor of popularization and application, and soil restoration effect is excellent without secondary pollution. After soil organism is degraded and restored, planting can realize again in the same year, yield is higher than that of normal soil, and resultantly, the soil ecological restoration conditioner is worthy of wide application.

The invention relates to the technical field of industrial wastewater treatment, in particular to a method for treatment of pickling wastewater with dunaliella salina and halophilic bacteria. The method includes: pretreatment of pickling wastewater, gradient acclimation of medium, construction of a bacterial-algal symbiotic system and collection of bacteria and algae. The method provided by the invention can effectively remove nitrogen, phosphorus and total nitrogen content in pickling wastewater, is not restricted by the salt concentration of wastewater during degradation treatment of high-salt wastewater, and has good nitrogen and phosphorus removal effect on pickling wastewater; the method is free of anaerobic or aerobic condition restriction, has no need for additional oxygen supply equipment, and also is independent of additional carbon sources; only under appropriate illumination and temperature conditions, can a good degradation effect be achieved; and the method has the advantages of low operating cost, green and environmental protection, etc.

The invention discloses a process for treating a high-salt wastewater biological film through halophilic bacteria YL5-2. According to the process for treating the high-salt wastewater biological filmthrough the halophilic bacteria YL5-2, the halophilic bacteria YL5-2 are used, wherein the halophilic bacteria YL5-2 are preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.16315. The high-salt wastewater treating process can be a biological film process or a symbiotic process of the biological film process and an activated sludge method process, and the treating effect under the process condition of suspended biological stuffing is optimal; and the process provides the new possibility for biological treatment and biological denitrification treatment of the high-salt wastewater with the salt concentration being 3-30%, especially 10-30%.

The invention provides a halophilic bacterium with the preservation number of CGMCC No.22795 and the preservation date of June 28, 2021. The invention further provides a preparation method of the halophilic bacterium, a method for producing PHA by culturing the halophilic bacterium with a low-salt culture medium and an application of the halophilic bacterium. In the method, the used halophilic bacterium can be obtained by breeding means of mutagenesis screening and molecular modification, and a method for producing PHA containing multiple monomers by adding a non-related carbon source (a substrate, such as glucose, which is not directly related to a microbial PHA structure) is realized in the low-salt culture medium, and the proportion of each monomer is adjusted according to the type of the carbon source. According to the method, the problems that traditional halophilic bacteria culture and separation steps are complicated, downstream high-salt sewage is difficult to treat and the like can be solved, and PHA is produced through open fermentation.

This method of restricting methane production in methanogenic bacteria, by the use of the enzyme and coenzyme inhibitors, works during anaerobic reductive dechlorination. Various compounds such as, but not limited to, red yeast rice, vitamin B10 derivatives, and ethanesulfonates are utilized to disrupt these different enzyme and coenzyme systems responsible for the production of methane. This method affects the competition of the methanogen and halo bacteria for the organic hydrogen donors that are injected in the soil and groundwater system during the remediation process.

This invention relates to a test tube slant culture method for rapidly and effectively screening Vibrio parahaemolyticus. The method comprises: (1) placing the collected Vibrio parahaemolyticus sample into a Fernbach culture flask containing liquid culture medium, and culturing by shaking in light; (2) collecting enrichment culture liquid by an inoculating loop, and laying a curve onto the prepared test tube culture medium slant; (3) culturing in a culture case to obtain individual slant colonies; (4) observing the slant, selecting a conoly characteristic of Vibrio parahaemolyticus, laying a curve onto the prepared test tube culture medium slant, culturing, repeating for 3 times, separating and purifying to obtain cultured Vibrio parahaemolyticus strain. The method adopts test tube slant culture method, and can realize rapid and effective screening of Vibrio parahaemolyticus. The efficiency can be raised by 3-4 times.