131 results about "Honey samples" patented technology

The invention relates to a rapid and convenient method for testing the quality of honey and the application thereof. The main steps are as follows: (1) a honey sample is collected; (2) the contents of water, glucose and fructose and the amylase value of the honey sample are obtained by a routine chemical method; (3) a near infrared spectrum of the honey sample is collected; (4) the preprocess of the near infrared spectrum is carried out, the interference factors are eliminated and the wavelength range is selected; (5) calibration models among the contents of water, glucose and fructose, the amylase value and the near infrared spectrum of the honey sample are respectively built and tested; (6) the near infrared spectrum of the sample to be tested is collected; (7) the contents of water, glucose and fructose and the amylase value of the sample to be tested are predicted by using the built models. The invention has the advantages of simple pretreatment, rapid analyzing speed, environmental protection, etc. Moreover, the contents of water, glucose and fructose and amylase value in the honey can be predicted with reliable and ideal result.

The invention provides a quick detection method for detecting the content of various compositions in honey by utilization of a mathematical model between a near infrared spectrum of the honey and the contents of the various compositions of the honey, in order to overcome the defects of complex operation and labor and time consumption in a chemical and chromatographic analysis method for detecting the quality of the honey. The quick detection method for the quality of the honey is as follows: firstly, obtaining a honey sample in the homogeneous fluid state; secondly, acquiring the near infrared spectrum; and thirdly, converting the near infrared spectrum acquired into the quality parameter of the honey by utilization of the mathematical model. The quick detection method for the quality of the honey can quickly and accurately detect the contents of water, soluble solid content, organic acid, fructose, glucose, sucrose and maltose in the honey.

The invention provides a method for distinguishing Chinese honeybee honey from apis mellifera honey based on major royal jelly protein ingredients. By means of a biological method and the difference between Chinese honeybee honey and apis mellifera honey in protein, different protein bands appear by conducting protein electrophoresis on a honey sample after the honey sample processed, protein categories are determined by conducting mass spectrometry on the different protein bands, and then the two kinds of honey from different bee species are identified. The problems that Chinese honeybee honey and apis mellifera honey available in market are hard to distinguish, and apis mellifera honey pretends to be Chinese honeybee honey or is mixed in Chinese honeybee honey are solved, and the situation that low-price apis mellifera honey pretends to be high-price Chinese honeybee honey can be inhibited. The method is used for distinguishing Chinese honeybee honey from apis mellifera honey.

The invention discloses a method for distinguishing between pure and adulterated honey based on combination of hydrogen nuclear magnetic resonance and a partial least square method. The method relates to food detection, and application of a hydrogen nuclear magnetic resonance fingerprint to food detection. The method comprises the following steps: firstly, building a pure honey database and a syrup-doped honey database respectively; secondly, building a distinguishing model, and carrying out a reliability test on the identification model; finally, distinguishing honey samples to be detected, and expanding the databases with the distinguishing results to improve the representativeness of the samples and the distinguishing capability. The method has the advantages that the technique is reliable, the operation is simple, a large quantity of samples can be treated in a short time, the dubious honey samples can be quickly screened out, the subjective factors and the personal errors can be avoided, and the conclusion can be scientific and reliable.

The invention discloses a honey adulteration detection method based on HPLC-ELSD and a partial least squares discriminant analysis method, and belongs to the technical field of food detection. The method comprises simple sample pretreatment, wherein the simple sample pretreatment comprises the steps of adopting a hydrophilic interaction chromatography separation-evaporation light scattering detector (HILIC-ELSD) method to analyze oligosaccharide and isomer components of oligosaccharide in authentic honey and a honey sample adulterated with fructose corn syrup or malt syrup, and utilizing difference of oligosaccharide component fingerprint spectrums of the authentic honey and the adulterated honey to conduct data processing through the partial least squares discriminant analysis method, so that adulteration identification is further achieved. The honey adulteration detection method based on the HPLC-ELSD and the partial least squares discriminant analysis method can effectively identify adulterated honey with C4 botanical syrup or C3 botanical syrup, the adopted analysis instrument is popular, and the method is convenient for popularization and application.

The invention provides a method for identifying the honey of Apis cerana and the honey of Apis mellifera. The method achieves the purpose of identifying the two kinds of honey based on the differenceof alkane components in the two kinds of honey. Honey samples are extracted with petroleum ether and then detected via gas chromatography-mass spectrometry; and detection results show that the alkanecomponents of the honey of Apis cerana and the honey of Apis mellifera are different, the honey of Apis cerana contains C37 alkanes, and the honey of Apis mellifera contains C31 alkanes. It is confirmed that the component difference is only related to bee species after detection and analysis of a large number of samples, and the influence of plant sources on identification is excluded. The differential alkane components are stable in honey and are not affected by concentration processing and shelf life. The method of the invention is reasonable in design, convenient, quick and accurate in identification, and is applicable to both the identification of the sources of raw honey and commercial bee species and the identification of the honey of Apis cerana blended with a certain proportion ofthe honey of Apis mellifera.

The invention provides a method for identifying apis cerana honey and apis mellifera honey, which achieves the purpose of identifying the apis cerana honey and the apis mellifera honey according to the specific band difference between the proteins of the apis cerana honey and the apis mellifera honey. After an apis cerana honey sample is treated, protein electrophoresis is conducted, the apis cerana honey has a 15.0KDa protein band, the apis mellifera honey has a 16.5KDa protein band, and a mixed honey sample of the both has both the 15.0KDa protein band and the 16.5KDa protein band. By differentiating the specific protein bands of the different honeys to achieve the purpose of identification, the method can accurately identify the apis cerana honey and the apis mellifera honey. In the method disclosed by the invention, the target proteins stably exist in the apis cerana honey and the apis mellifera honey, not affected by honey resources, concentration processing and shelf life. The design of the method disclosed by the invention is reasonable and simple, instruments are common, cost is low, identification is accurate, and the method is also suitable for identifying a certain proportion of apis mellifera honey mixed in the apis cerana honey.

The invention relates to a method for building a model for DPLS-BS-UVE rapid honey identification, and belongs to the field of food safety. The method comprises the steps that a series of honey, syrup and adulterated honey samples are collected at first for nuclear magnetic resonance spectrum analysis, DPLS-BS-UVE analysis is adopted after data extraction and pretreatment, honey adulterated markets are screened, a multiple linear regression equation is built by using the area of the spectral peak corresponding to the markets as an X variable and using the sample type as a Y variable, and the method is used for rapid honey adulteration identification. The inherent thinking in the prior art that only one kind or category of matter is used as the identification market is broken through, and the identification method having the advantages of being short in analysis time, strong in integrity, good in specificity, high in controllability, high in accuracy and the like is obtained.

The invention relates to the technical field of analysis and detection, in particular to a method for identifying honey varieties based on an attenuated total reflection terahertz dielectric spectrum. The method comprises the steps of at first conducting terahertz scanning on a honey sample of which variety is known and obtaining a terahertz time-domain spectrogram, then transforming the terahertz time-domain spectrogram into a terahertz frequency domain spectrogram through Fourier transformation, conducting calculation to obtain the terahertz dielectric spectrum of the honey sample of which variety is known and conducting principal component analysis according to the terahertz dielectric spectrum to establish a PCA-DA model of the honey sample of which type is known; finally adopting the same method to conduct detection on honey sample to be tested to obtain a terahertz dielectric spectrum, then conducting identification on the terahertz dielectric spectrum according to the PCA-DA model, and the variety identified by the PCA-DA model is the variety of the honey of which the variety is unknown. According to the method for identifying honey varieties based on the attenuated total reflection terahertz dielectric spectrum, only terahertz spectrum of the unknown honey needs to be collected, and then identification can be conducted through the model; meanwhile, the method for identifying honey varieties based on the attenuated total reflection terahertz dielectric spectrum has the characteristics and advantages that detection speed is high, operation is convenient, no chemical agent is polluted, both time and labor are saved, and accuracy is high.

The invention provides an enzyme linked immunosorbent assay kit for detecting a trimethoprim medicament and application thereof. The enzyme linked immunosorbent assay kit comprises an enzyme label plate coated with envelope antigen, an enzyme marker, trimethoprim specificity antibody working solution (when the enzyme label plate is coated with the envelope antigen and the enzyme marker is enzyme-labeled anti-antibody or the enzyme label plate is coated with the envelop antigen and the enzyme marker is the enzyme-labeled antigen), trimethoprim standard solution, substrate developing solution, stop solution, concentrated washing solution and composite solution. The invention also discloses a method for detecting the trimethoprim by applying the enzyme linked immunosorbent assay kit. The method comprises the following steps of: performing sample pretreatment; detecting by the kit; and analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the residual amount of the trimethoprim in a honey sample, has the advantages of simple operation, low expense and high sensitivity, can be monitored in field, and is suitable for screening mass samples.

The invention discloses an analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey, and particularly relates to a method by applying the ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique combining metabonomics. The method comprises the steps that firstly, a true Chinese date honey sample and a syrup-adulterated with fake Chinese date honey sample to be detected are pretreated by organic solvent respectively, separation and determination of chemical components in the pretreated samples are achieved by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrometry method, then obtained UHPLC-MS original data of the true Chinese date honey sample and the Chinese date honey sample to be detected is preprocessed, and finally true Chinese date honey and syrup-adulterated with fake Chinese date honey are distinguished by applying principal component analysis of a multivariate statistical analysis method. According to the analytical method, after metabolin information of Chinese date honey and syrup-adulterated with fake Chinese date honey is obtained comprehensively, combined with multivariate statistical analysis, Chinese date honey and syrup-adulterated with fake Chinese date honey are analyzed comprehensively, and the detection of syrup-adulterated with fake Chinese date honey is completed.

The invention discloses a method for detecting antibiotic residue in honey based on a rapid classification extraction technology, which belongs to the technical field of honey quality detection. The method comprises the following steps of: firstly establishing a standard atlas library of blank honey and a standard atlas library of antibiotic residue after accurately adding one antibiotic, then comparing a detecting image of the to-be-detected honey sample with the standard atlas library of the antibiotic residue to judge whether the antibiotic content in the to-be-detected honey sample exceeds the standard. Partial sample containing pollutant can be rapidly identified in low cost by using the method, the extraction work load is greatly reduced, the extraction time of the pollutant is shortened, the detection efficiency is improved and the detection cost is reduced; a rapid pollutant detection monitoring technology system in the honey in high throughput and low cost for honey export enterprises is established, the defects that the pollutant detection efficiency is low, the detection target is single, the price is expensive and the detection time is long are changed; therefore, the method can provide technical support for the safety production of the exported honey.

The invention provides a method for quickly identifying honey varieties and adulterated honey. The method comprises determining the fluorescence emission spectrum of a honey sample and comparing response values of characteristic peaks and overtones based on a blank solution as a control to determine whether the honey is adulterated, wherein the response value is represented by peak height. The method only utilizes a fluorescence spectrophotometer. The fluorescence spectrophotometer cannot be equipped with a high-pass filter or other device for filtering overtones. The method can acquire a spectrum, can be operated through simple and fast processes, realizes detection time of only 5min from pretreatment to result production and is easy to operate.

The invention discloses analysis method for distinguishing realhoney fromsyrup adulteratedhoney througha chromatography mass spectrometry technology in combination with a metabonomics method. The method comprises the following steps: performing pretreatment on a real honey sample and to-be-detectedsyrup adulteratedhoney with the same honey source of real honeyby adoptingan organic solventrespectively, separating and measuring chemical components in the samples subjected to pretreatment through an ultra-high performance liquid chromatography-high resolution mass spectrum, then preprocessing UHPLC-MS original data of the obtained real honey sample and the to-be-detected honey sample, and finally analyzing and distinguishing realhoney fromsyrup adulteratedhoney througha multivariate statistical analysis method PCA. The analysis method is superior to other detection methods, does not need qualitatively and quantificationally measuring one certain or certain targeted matter, and is a macroscopic and non-target method for varietydistinguishment.

The invention discloses a rapid honey detection test strip, and a preparation method and application thereof. The preparation method includes (1), preparing and purifying polyclonal antibodies SP1 and SP2 capable of specifically recognizing MRJP1; (2), preparing a collaurum solution; (3), coating a nitrocellulose membrane with a quality control line (line C) and a detection line (line T); (4), assembling the rapid honey double-antibody sandwich detection test strip taking the bee endogenous protein MRJP1 as a biomarker; (5), preparing a honey detection sample. After separated and purified protein in the honey sample reacts with the gold-labeled test strip, authenticity of the honey sample can be judged according to color development conditions of the line T. The gold-labeled honey double-antibody test strip is applicable to detection of fake honey pretend by artificial syrup, and a new reliable rapid detection method is provided for quality and authenticity detection of honey.

The invention discloses a method for identifying authenticity of honey. The method comprises the following steps: 1, enriching high-polymerization degree oligosaccharides; 2, separating the oligosaccharides by thin layer chromatography, and qualitatively analyzing a honey sample; 3, scanning by using a scanner, and then analyzing adulteration of the honey sample by using Image J software. By the method, in allusion to a falsification way of adulterating a fructose syrup into honey, the authenticity of the honey is identified through qualitative and quantitative analysis on the oligosaccharideshaving the degree of polymerization being greater than five in the honey.

The invention discloses a rapid detecting method for hydroxymethyl furfural (HMF) in honey. The rapid detecting method comprises the following steps: (1) preparing a sample, namely accurately weighing and adding 7.5g of honey sample into a small beaker, adding 10mL of distilled water, uniformly mixing the distilled water and the honey sample by a glass rod to obtain a mixture, transferring the mixture to a 25mL volumetric flask, adding distilled water to make up to volume, and uniformly shaking; (2) developing color, namely taking and adding 1.00ml of a to-be-detected sample into a graduated test tube with a stopper, then, adding 3mL of mixed solution into the test tube, and developing color for 5 minutes after uniformly mixing; (3) detecting, namely comparing the color of a developed solution with a color chart to determine the content of HMF. The rapid detecting method for hydroxymethyl furfural in honey disclosed by the invention has characteristics of a simple used reagent, no complex condition and apparatus equipment, simplicity and convenience in operation and rapidness.

The invention discloses a method for rapidly detecting the amylase value in honey. The method comprises the following steps: 1, sample preparation: accurately weighing 5.0g of a honey sample, putting the honey sample in a beaker, adding 7.5mL of an enzymatic hydrolysis buffer solution and 10mL of distilled water, stirring until the sample is completely dissolved, transferring the above obtained solution to a 25mL volumetric flask, adding distilled water to a constant volume, and shaking up; 2, enzymatic hydrolysis: accurately sucking 2.5mL of the prepared sample solution to a test tube with 12.5mL of a starch solution, uniformly mixing, and carrying out enzymatic hydrolysis at normal temperature for 10min or 20min; 3, coloration: sucking the obtained enzymatic hydrolysis liquid to a colorimetric pipe with a coloration solution and distilled water, and coloring at normal temperature for 5min; and 4, detection: comparing the color of the coloration solution with a multicolor colourimetric card, and determining the amylase value in the honey. The method is characterized in that amylase reacts with starch at normal temperature, and a colourimetric card technique is cooperated to detect. The method has the advantages of simple operation, simple conditions, rapidness and the like.

The invention belongs to the field of testing and provides a detection method of volatile components in honey. The detection method comprises the following steps that (1) a honey sample is weighed and the honey sample is put into a headspace bottle; an extraction head of a solid-phase micro-extraction device is inserted into the headspace bottle; (2) control conditions of an automatic thermal desorption system are set as follows: the starting temperature is 35 DEG C, the keeping time is 1 minute and the time is delayed for 3 minutes; (3) gas chromatography conditions are set as follows: the initial temperature of a capillary-column chromatography is 40 DEG C, the temperature is kept for 2 minutes and the temperature of a sample inlet is 250 DEG C; and (4) mass spectrometric detection: qualitative and quantitative detection of the volatile components is carried out. A headspace solid-phase micro-extraction technology and a gas chromatography-mass spectrometry technology are used for analyzing the volatile components. According to the method provided by the invention, the extraction efficiency of the volatile components in the honey and the accuracy of an analyzed result are greatly improved; and a certain theoretical basis can be provided for disclosing whole information of the volatile components of different honey samples and evaluating the authenticity of the honey.

The invention discloses a method for identifying unifloal honey, which comprises the following steps: establishing a standard sample of the unifloal honey, measuring data of basic physical and chemical indicators of a honey sample to be measured, measuring data of characteristic matter components of the honey sample to be measured, and judging whether the honey to be measured is the unifloal honey by using principal component analysis and cluster analysis. The method is applicable to the identification whether the honey is true and whether the unifloal honey is mixed with other low-quality honey, and the identification of actual nectariferous plant nectar sources of the honey, and is suitable for differing the unifloal honey from other unifloal honey.

The invention relates to a rice syrup extractive, which has the structure as shown in formula I. The invention also relates to the application of the extractive in rice syrup adulterated honey detection. The invention further relates to a detection method for rice syrup adulterated honey, which takes the rice syrup extractive as a target material, and the honey which is detected with the target material is the rice syrup adulterated honey. The detection method takes the rice syrup extractive as an identification material, only a small mount of sample can detect the material, and the detectionaccuracy rate is high. The method is simple to carry out, and has wide application value.

The invention provides an enzyme-linked immunoassay kit for detecting a fluoroquinolone medicine. The enzyme-linked immunoassay kit comprises an enzyme-labelled plate coated with a coating antigen, a standard solution of the fluoroquinolone medicine, an enzyme-labelled bivalent antibody, a specific antibody of the fluoroquinolone medicine, a developing solution of a substrate, a stop solution, a washing solution, a combination solution and a diluent of a honey sample; the coating antigen is a coupled antigen of the fluoroquinolone medicine; and the enzyme-labelled bivalent antibody is an enzyme-labelled anti-goat and anti-rat antibody. The invention further discloses a method for detecting the fluoroquinolone medicine by using the enzyme-linked immunoassay kit. The method comprises the following steps: pre-processing a sample at first, then, detecting by using the kit, and finally, analyzing a detection result. The enzyme-linked immunoassay kit provided by the invention can be used for detecting the content of the fluoroquinolone medicine in honey, chicken, pork, fish, shrimp, milk, milk powder and egg samples, is simple and convenient to operate, low in cost and high in sensitivity and can be used for monitoring on site and applied to screening of a lot of samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting nitroimidazole drugs. The kit comprises an enzyme labeling plate coated with metronidazole coupling antigen, enzyme labeling antiantibody working solution, specific antibody working solution of the nitroimidazole drugs, metronidazole standard substance solution, substrate developing solution, stop solution, concentrated washing solution and redissolving solution. The invention further discloses a method for detecting the nitroimidazole drugs by utilizing the enzyme linked immunosorbent assay kit. The method comprises the following steps of: firstly, carrying out sample preprocessing; secondly, carrying out detection by utilizing the kit; and thirdly, analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of the nitroimidazole drugs in a honey sample, is simple and convenient to operate, is low in cost, can realize on-site monitoring and is suitable for screening of a large number of samples.

The invention relates to the field of food detection, and particularly discloses an application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey. Meanwhile,the invention provides a method for identifying the apis cerana honey and the apis mellifera honey by taking the content of the 3-amino-2-naphthoic acid as an identification basis. The method comprises the steps of: if the content of 3-amino-2-naphthoic acid in a honey sample is between 17.59-620.01 mu g / kg, judging that the honey sample is apis cerana honey; and if the content of 3-amino-2-naphthoic acid in the honey sample is between 0.04-12.00 mu g / kg, judging that the honey sample is apis mellifera honey. The method provided by the invention is simple, convenient, accurate and convenient to operate and popularize, and has important practical significance for protecting legal rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

The invention discloses an analysis method of identifying black locust honey and syrup adulterated black locust honey, and particularly relates to a method of combining an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique and metabonomics. The analysis method comprises the following steps: respectively pre-processing a genuine black locust honey sample and syrup adulterated black locust honey to be detected by adopting an organic solvent, realizing the separation and determination of chemical components of the preprocessed sample by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum method, then preprocessing the obtained UHPLC-MS original data of the genuine black locust honey sample and the black locust honey sample to be detected, and finally analyzing main components by using a multivariate statistical analysis method so as to identify the black locust honey and the syrup adulterated black locust honey. The analysis method has the beneficial effects that the black locust honey and the syrup adulterated black locust honey are comprehensively analyzed by multivariate statistical analysis after the information of metabolites of the black locust honey and the syrup adulterated black locust honey are comprehensively obtained, thereby completing the detection of the syrup adulterated black locust honey.

The invention discloses a method for detecting honey sources. The method comprises the following steps of: (1) measuring a honey sample into a sample groove special for a rheometer, submerging a rotor of the rheometer into honey, setting the shearing rate range of the rotor and measuring the viscosity value of the honey by using the rotor; (2) adjusting the temperature of a rheometer water bath and measuring the viscosity values of the honey at different temperatures respectively by using the rotor; (3) establishing a main component analysis and clustering analysis mode recognition model by using the viscosity values of the honey sample at different temperatures to perform qualitative analysis on the honey sources; and (4) marking an encoding value for predicting respectively for honey ofdifferent honey sources and establishing a main component regression analysis predictive model by using the viscosity values of the honey sample at different temperatures to perform predictive analysis. During the implementation of the method, preprocessing is not required, so that the experiment steps are simplified, and the reliability and repeatability are enhanced greatly.

The invention relates to a beta-fructofuranosidase enzyme-linked immunoassay detection kit which comprises a beta-fructofuranosidase standard enzyme solution, an enzyme label plate enveloped with a beta-fructofuranosidase monoclonal antibody, a sample diluent, a beta-fructofuranosidase rabbit polyclonal antibody working solution, an enzyme labelled secondary antibody working solution, a substrate coloured solution, a washing concentrate and a stop solution. For the kit disclosed by the invention, the content of beta-fructofuranosidase in a honey sample is measured by adopting a double antibodies sandwich ELISA (enzyme-linked immuno sorbent assay) method; a linearly dependent coefficient of a dosage-reaction curve is more than 0.980; R2 is equal to 0.9993; the sensitivity is 10U / KG; and the national ELISA detection quality requirement is met; and an intra-plate variation coefficient (CV%) of the kit is less than 10 percent. The kit has stable measurement result and good precision. The kit disclosed by the invention has the advantages of simpleness in processing the sample, high detection speed, short detection time of only 2.5 hours, sensitivity, accuracy and high throughput, and provides an effective means for detecting counterfeiting of honey.

The invention provides a method for identifying high-fructose-syrup adulterated honey. The method takes an alpha-dicarbonyl compound as a target substance to detect a honey sample containing the target substance, i.e., the high-fructose-syrup adulterated honey. The alpha-dicarbonyl compound with an indication effect comprises one or more of 1,2-diketone-pentose, 2,3-diketone-pentose, 3-deoxy-1,2-diketone-pentose and 1-deoxy-2,3-diketone-pentose. According to the method provided by the invention, four detected and screened alpha-dicarbonyl compounds are used as a target compound for identifyingvarious types of high-fructose-syrup adulteration. The method is simple and effective and the main high-fructose-syrup adulterated honey at present can be covered through one time of analysis.

The invention relates to a method for distinguishing distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey. The method comprises the steps of (1) weighing a detected amount of honey sample to be detected; (2) detecting volatile hydrocarbon compounds in the honey to be detected by using HS-SPME-GC-MS to obtain a detection spectrogram, and quantifying Cis-9-Tricosene, (C9T), isomers thereof, namely, Iso-9-Tricosene, Cis-9-Pentacosene, (C9P) and an isomer Iso-9-Pentacosene in the honey sample to be detected according to the spectrogram; and (3) identifying the Apis cerana cerana honey and Apis mellifera ligustica honey according to the contents of C9T and C9P in the honey and the proportions of C9T and C9P to respective isomers.

The invention belongs to the test field, and proposes a method for optimizing the extraction of volatile ingredients in honey. The method comprises the following steps: (1) sealing a honey sample in a head space bottle, sampling by using an extraction head, and performing gas chromatographic analysis on the sample; (2) based on single-factor tests, determining the factors and the level of Plackett-Burman tests; (3) performing a response surface test, using a factor which influences a response value remarkably and is obtained through screening as an analytical factor, using a total peak area of a chromatogram as a response value Y, and determining the optimum conditions of solid-phase microextraction. According to the invention, jujube honey is used as an experimental material, the total peak area in a gas chromatography peak is used as an inspection index, a Plackett-Burman design and a response surface method are used for optimizing the extraction technique for the volatile ingredients of the honey, after the extraction technique is optimized, the extraction efficiency is high, an optimizing model is reasonable, and definite methodological guidance can be provided for further qualitative and quantitative analysis of the volatile ingredients in the honey with different honey sources.