103 results about "IgG.monoclonal" patented technology

The invention discloses a colloidal gold kit for jointly detecting coronavirus IgM / IgG antibody, and a preparation method thereof, and relates to the field of biological medicine. Whether anti-novel coronavirus nucleocapsid protein IgM antibody and / or anti-novel coronavirus nucleocapsid protein IgG antibody exists in human serum or plasma or not by adopting an antigen-antibody sandwich method anda colloidal gold immunochromatography method principle, the novel coronavirus nucleocapsid protein containing 6xHis mark is marked by applying colloidal gold, thereby forming gold-marked N protein tobe adsorbed on a gold-marked pad, the novel coronavirus nucleocapsid protein containing 6xHis mark is used as an indication marker, the mouse-anti-human u chain monoclonal antibody is coated on the IgM detection line of a NC membrane, the mouse-anti-human IgG monoclonal antibody is coated on the IgG detection line and the mouse-anti 6xHis monoclonal antibody is coated on a quality control line ofthe NC membrane, the qualitative detection of the anti-novel coronavirus nucleocapsid protein IgG antibody is realized, and the colloidal gold kit disclosed by the invention has the advantages of being convenient to use, high in sensitivity and short in detection time.

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

The invention provides a novel coronavirus detection test strip as well as a preparation method and application thereof. The test strip comprises a binding pad and an analysis membrane, and the binding pad is coated with a luminescent substance labeled 2019-nCoV recombinant antigen and a mouse anti-human HCG monoclonal antibody; a T2 detection line, a T1 detection line and a quality control line are sequentially arranged on the analysis membrane along the chromatography direction; the T2 detection line is coated with a mouse anti-human IgG monoclonal antibody, the T1 detection line is coated with a mouse anti-human IgA monoclonal antibody and a mouse anti-human IgM monoclonal antibody, and the quality control line is coated with a goat anti-mouse IgG polyclonal antibody. The test paper card can simultaneously determine the positive conditions of IgA antibody, IgM antibody and IgG antibody in serum of a patient, can more accurately detect the early antibody level condition in the body of the patient, assists in judging different periods of novel coronavirus infection of the patient, and improves the sensitivity and specificity of novel coronavirus detection.

The present invention discloses a kit and method for detection of thyroid peroxidase antibody. The kit includes thyroid peroxidase antibody series standards, a magnetic separation reagent of a magnetic particle suspension conjugated with streptavidin and pigment, a first reagent of an antigen solution containing biotin N-hydroxysuccinimide ester labeled thyroid peroxidase antigen, a second reagent of a solution containing alkaline phosphatase labeled mouse-anti-human IgG monoclonal antibody. The kit is used for detection of thyroid peroxidase antibody. By the above-described manner, the invention uses a biotin-streptavidin signal amplification system and alkaline phosphatase labeling to solve the traditional problem of low ELISA sensitivity; the nanometer magnetic particle separation system achieves high sensitivity for radioimmunoassay (RIA), but has no radioactive contamination, and has greatly improved period of validity, security and environmental performance.

The invention discloses a kit for detecting pig pseudorabies. The kit comprises an assay plate coated with pig pseudorabies virus gE antigen, a rabbit anti-pig IgG antibody marked by HRP (horseradish peroxidase), fast coating buffer, fast blocking buffer, sample diluents, TMB substrate, stop buffer, 20 times wash solution, negative control, and a positive control. Through adopting pig pseudorabies virus specificity gE antigen with high purity and high activity expressed through gene engineering to coat the assay plate, and through adopting HRP-conjugate of rabbit anti-pig IgG monoclonal antibody containing HRP, the kit for detecting pig pseudorabies, provided by the invention, has the advantages of strong specificity, high sensibility, less susceptibility to misjudge of false positive or false negative and the like; moreover, the kit is simple in structure, low in detection cost, convenient and fast in operation and strong in timeliness, has a wide market prospect, and can create larger social benefits.

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

The invention discloses an animal brucella antibody gold mark rapid detection kit and belongs to the field of animal disease detection. The kit consists of two parts namely a card box and a test strip, wherein the card box comprises a box cover (4) and a box bottom (7), and the detection test strip is placed in the card box; a sampling hole (1) and an inspection window (2) are formed in the front side of the card box, and a sampling hole label (5), a result judging specification (6) and an information recording column (3) are printed on the front side of the box cover. According to the invention, the colloidal gold immunochromatography technology is adopted, a brucella BP26, OMP25 or OMP31 pathogen in engineering expression is used as the detection pathogen, a mouse anti-bovine IgG monoclonal antibody, a mouse anti-goat IgG monoclonal antibody or a mouse anti-swine IgG monoclonal antibody is used the capture antibody, and an indirect process detection kit is made. The animal brucella antibody gold mark rapid detection kit can be used for detecting the brucella antibody in the serum, the plasma or the whole blood of cattle, goats or swines and has the advantages of simple detection method, accurate and rapid result display and no requirement of special instruments or equipment.

The invention discloses an immunochromatographic test strip for rapidly detecting malaria and a preparation method thereof. The test strip is formed by sticking a sample pad, a labeling pad, a coating membrane and absorbent paper to a substrate in sequence through lap joint, wherein colored latex particle labeled plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody are coated on the labeling pad; the coating membrane comprises detection regions and a control region; the detection regions are coated by plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody with epitopes different from the epitopes of the monoclonal antibodies on the labeling pad; and the control region is coated by an anti-mouse IgG (immunoglobulin G) monoclonal antibody. The test strip improves the accuracy and convenience of malaria screening, is simple and convenient to operate and has the advantages of rapidness, simpleness, convenience and intuition.

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.

The invention discloses a preparation method of human immunodeficiency virus (HIV) antibody detection test paper. The preparation method comprises the following steps of: spraying gold on a gold cushion by a mixed solution of a P74 recombinant antigen mark and a P44 recombinant antigen mark through membrane marking and gold spraying equipment to obtain a first gold cushion; spraying gold on the gold cushion by a rabbit IgG monoclonal antibody mark to obtain a second gold cushion; marking on a polyvinyl chloride bottom plate stuck with a nitrocellulose membrane by a detection line covering solution and a control line covering solution to obtain a polyvinyl chloride bottom plate with a marked membrane; assembling a sample cushion, the first gold cushion, the second gold cushion, the polyvinyl chloride bottom plate with the marked membrane and water absorption paper; and cutting the assembly into the detection test paper. In the manner, the detection test paper prepared by the preparation method disclosed by the invention can be used for judging whether an oral cavity sample contains an HIV antibody or not based on an immune lateral flow chromatography technology through manual operation and naked eye reading; the diagnosis is rapid, the result is accurate, and damages to a body of a subject are not caused.

The invention relates to a dog rabies virus NP-ELISA antibody detection kit, wherein, an antibody detection plate is a detachable 96-hole enzyme labeled plate which is coated by rabies virus nucleoprotein NP, enzyme conjugate working liquid is a horseradish peroxidase labeled anti-dog IgG monoclonal antibody, a positive control is dog rabies standard positive serum, a negative control is a dog standard negative serum, the rabies virus nucleoprotein NP is a fragment of rabies virus ERA vaccine strain NP gene 1350bp which is amplified by RT-PCR technology, the rabies virus nucleoprotein NP is inserted into the downstream of a prokaryotic expression vector pET-28a to construct a pET-NP recombinant expression plasmid, Escherichia coli BL21 DE3 is converted by the calcium chloride method, 0.01mM IPTG induced expression is carried out, ultrasonic waves are used for the lysis of an expression strain, and the purification by a Ni-NTA affinity chromatography column is carried out for the preparation. The using concentration of the rabies virus nuclear NP recombinant protein for coating the NP antibody detection plate is 2 mug / ml, and the using amount is 100 mu 1 / hole. The dog rabies virus NP-ELISA antibody detection kit has the advantages of strong specificity, high sensitivity and good safety.

The invention relates to a test paper strip for (PCT / SAA) combined rapid detection of human procalcitonin / serum amyloid protein A. The test paper strip comprises a detecting card shell, a test strip, a sample pad, a gold conjugate pad coated with two colloidal gold labeled antibodies, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane is provided with a T1 detection line coated with a solid-phase matched anti-SAA monoclonal antibody, a T2 detection line coated with a solid-phase matched anti-PCT monoclonal antibody, and a control line C which is parallel to the detection line and coated with a goat anti mouse IgG monoclonal antibody. Two kinds of colloidal gold labeled antibodies are provided, which are respectively a colloidal gold labeled antibody which can be specifically combined with a to-be-detected antigen PCT and an antibody which can be specifically combined with a to-be-detected antigen SAA. The test paper strip disclosed by the invention can be used for simultaneously and rapidly detecting the PCT / SAA in a patient sample in a combined manner and has the advantages of improving the diagnosis accuracy of early inflammatory reaction of infectious diseases and being simple to operate, rapid and convenient.

The invention relates to a kit for quickly detecting a swine fever antibody. In the method, a pair of specific primers is designed, a relatively conservative gene sequence is cloned, an antigen aiming at a swine fever E2 protein is expressed through a pronucleus expression technology, and, on the basis, the kit containing an enzyme linked plate coated with a high-purity and high activity swine fever virus specificity antigen, an enzyme conjugate of rabbit-anti-swine monoclonal antibody containing an HRP (Horseradish Peroxidase) marker, a TMB (Tetramethylbenzidine) color developing liquid and the like is prepared. The kit can quickly detect the swine fever antibody in blood serum or blood plasma and has strong specificity and high sensitivity.

The invention discloses an anti-double-stranded DNA antibodies IgG chemiluminescence immunoassay kit. The kit comprises biotinylated double-stranded DNA antigens, streptavidin-coated magnetic particles, acridine ester-labeled murine anti-human IgG monoclonal antibodies, anti-double-stranded DNA antibody IgG scaffolds, pre-excitation fluid and excitation fluid. Moreover a preparation method of the anti-double-stranded DNA antibodies IgG chemiluminescence immunoassay kit is also disclosed. Compared with the current kit the kit has the advantages of simple operation, high sensitivity and wide detection scope and the like.

An improved double antigen sandwich immunoassay method aims to lower the false positive rate of the prior double antigen sandwich immunoassay method and improve the specificity of detection. The method can be applied to various detection methods such as rapid diagnosis, enzyme linked immune detection, chemiluminescent detection, and the like through adding an antihuman IgG monoclonal antibody or polyclonal antibody in a reaction system of the double antigen sandwich immunoassay method according to a certain proportion to participate in detecting. Compared with the prior double antigen sandwich immunoassay method, the double antigen sandwich immunoassay method of the invention remarkably lowers the detection background and the false positive rate and greatly improves the specificity.

The invention discloses a hepatitis E virus IgG antibody detection kit. The kit includes the following components: a solid material coated with a hepatitis E virus (HEV) gene recombinant antigen, a mouse anti human IgG monoclonal antibody labeled with a signal generation substance, a concentrated washing solution, an enhancing liquid, an analysis buffer liquid, a sample diluent, a positive control and a negative control. In addition, the invention also provides a preparation method of the kit. The preparation method includes the following steps: a first step, preparing the solid material coated with the HEV gene recombinant antigen; and a second step, labeling a mouse anti human IgG monoclonal antibody with the signal generation substance. In addition, the invention also discloses an application of the kit in detection of a hepatitis E virus IgG antibody. The detection kit overcomes the defect of labeling antibodies with macromolecules (such as enzymes), has the advantages of high sensitivity, good stability, low cost and the like, can significantly improve the specificity, sensitivity and stability of detection of the hepatitis E virus IgG antibody, and besides, significantly reduces the cost.

The invention aims at providing a simple and quick Zika virus detection kit. The kit optimally selects fusion expression protein as diagnostic antigen; an anti-human IgG monoclonal antibody A374, an anti-human IgM monoclonal antibody A371 and a biotin-BSA conjugate are respectively coated on a nitrocellulose membrane as a detection line and a quality control line; colloidal gold labeled fusion expression protein and colloidal gold labeled streptavidin and other reagents are matched; and an immunochromatography capture method principle is used for qualitative detection of Zika virus specific IgM antibody and IgG antibody in human serum, thereby realizing quick and specific diagnosis of Zika virus infection.

The invention provides an AIDS (HIV-1 / 2) saliva rapid detection reagent, which is to fix a purified recombinant HIV-1 / 2 antigen on a fibrous membrane by adopting a principle of double antigen sandwich method. When the reagent is used, saliva to be detected is added. If a sample contains anti HIV-1 / 2 specific antibody, the antibody is combined with a corresponding antigen on the surface of the membrane to form a composite material. The composite material is captured by an antihuman-IgG monoclonal antibody to form a sandwich substance of the Au-HIVAg-HIV antibody-antihuman-IgG monoclonal antibody, and a purple strip is shown up. A negative sample cannot form the sandwich substance. The AIDS (HIV-1 / 2) saliva rapid detection reagent has high sensibility, better specificity and stability, has convenient operation without an instrument, and can detect AIDS virus in time by single portion. Detection processes can be reduced into one step and can be completed within 5 minutes, and the reagent can be applied to hospitals, epidemic stations and the Entry-Exit Inspection and Quarantine Bureau for field disease diagnose, and home self detection. The reagent uses saliva for directly detecting AIDS to avoid cross infection of blood and completely overcome the defect of laboratory detection technology, can carry out detection without a wound, has high speed, convenience and safety, and fills up domestic blank.

The invention discloses detection test paper for quickly diagnosing the Lyme disease, and a preparation method thereof. A method for detecting the Lyme disease by ELISA (Enzyme Linked Immunosorbent Assay) is long in detection time and is not suitable for substrate detection. The colloidal gold immunochromatography detection test paper for quickly diagnosing the Lyme disease comprises a bottom plate, a sample cushion, a Jinbiao cushion, a nitrocellulose membrane and a water adsorption cushion, wherein the sample cushion, the Jinbiao cushion, the nitrocellulose membrane and the water adsorptioncushion are arranged and connected in sequence and are all arranged on the bottom plate; the nitrocellulose membrane is provided with a first detection line, a second detection line and a quality control line, wherein the first detection line is provided with a mouse anti-human IgM (Immunoglobulin M) monoclonal antibody, the second detection line is provided with a mouse anti-human IgG (Immunoglobulin G) monoclonal antibody, and the quality control line is provided with a goat anti-mouse IgG polyclonal antibody; and the Jinbiao cushion is provided with Lyme disease recombinant antigen-colloidal gold conjugate. The detection test paper has the advantages of short detection time, high detection accuracy, high specificity and convenience in operation, and does not need the help of other equipment instruments.

The invention discloses a new method for large-scale high-efficiency purification of a conjugate of water soluble nano silver particles and a mouse-origin IgG monoclonal antibody, and belongs to the field of biotechnology. In allusion to the disadvantages that a conventional nano silver particle-antibody conjugate has complex purification process and low recovery rate and difficultly realizes large-scale production, glucosamine is adopted to seal residual carboxyl of the nano silver particle-antibody conjugate, a surface zeta potential of the conjugate is reduced; by adjusting the pH value of a solution to 4.5-5.0, the net charge content of the conjugate in the solution is further reduced, and the large-scale high-efficiency purification of the nano silver particle-antibody conjugate is achieved by an ordinary high-speed centrifugation method. The method simplifies experimental operation procedures of the nano silver particle-antibody conjugate, reduces requirements on separation equipment, is suitable for large-batch purification of the conjugate of the nano silver particles and the mouse-origin IgG monoclonal antibody, allows the yield to be more than 90%, and allows optical characteristics and biological activity of the conjugate to have no significant changes.

The invention relates to a Zika virus NSI antigen and the application of Zika virus NSI antigen in preparing a fluorescence immunochromatographic reagent. The amino acid sequence of the Zika virus NS1antigen is shown as SEQ ID NO. 1. The prepared fluorescence immunochromatographic reagent is a fluorescence immunochromatographic reagent for detecting a Zika virus antibody. A sample pad, a marker pad, a coating pad and an absorption pad are sequentially overlapped and adhered to a bottom lining card, and the marker pad is a glass fiber coated with a fluorescent latex microsphere labeled mouse anti-human IgG monoclonal antibody, and the coating pad is a nitrocellulose membrane coated with a goat anti-rabbit-mouse IgG antibody as a quality control line and a detection line coated with the Zika virus NS1 antigen. The Zika virus NS1 antigen provided by the invention has a better expression effect and is used for preparing the fluorescence immunochromatographic reagent for detecting a Zika virus IgG antibody, and a detection result can be observed by naked eyes after irradiation by ultraviolet rays.

The invention relates to a hybridoma cell strain and a monoclonal antibody secreted by the same. The antibody can be specifically bound with human IgG. The invention further relates to a kit comprising the hybridoma cell strain or the monoclonal antibody. The monoclonal antibody disclosed by the invention shows good performances in the aspects of antibody purity, repeatability, antibody valence and stability.

The invention relates to a hybridoma cell strain and a monoclonal antibody secreted by the same. The antibody can specifically bind with human IgG. The invention further relates a kit comprising the hybridoma cell strain or the monoclonal antibody. The monoclonal antibody is good in antibody purity, repeatability, antibody valence and stability.

The invention discloses detection paper for simultaneously detecting IgG and IgM of the novel coronavirus and a method. A nitrocellulose membrane in the detection paper is provided with a first detection strip sprayed with an anti-human IgM monoclonal antibody, a second detection strip sprayed with an anti-human IgG monoclonal antibody and a quality control strip sprayed with a monoclonal antibodyof the specific protein of anti-novel coronavirus 2019-nCoV, wherein the first detection strip, the second detection strip and the quality control strip are distributed at intervals; a conjugate padis sprayed with rare earth ion nanosphere labeled specific protein of novel coronavirus 2019-nCoV. The test strip can detect the IgG and IgM contents of novel coronavirus 2019-nCoV at the same time, which provides convenience for the clinical diagnosis of novel coronavirus 2019-nCoV.

The invention discloses a tri-antibody sandwich ELISA (enzyme-linked immunosorbent assay) detection method of the IgG (Immunoglobulin G) of a tree shrew, which is applicable to the detection of the total content of IgG in the blood plasma of the tree shrew. The method comprises the following steps: coating an enzyme-label plate with the monoclonal antibody in the anti-rat IgG of the tree shrew as a capture antibody, and combining with the IgG of the tree shrew to be detected; and establishing the tri-antibody sandwich detection method of the IgG of the tree shrew by optimizing the concentration of the antibody and other multi-factor conditions by using the polyclonal antibody of the anti-rabbit IgG of the tree shrew as the secondary antibody and the commercial goat anti-rabbit IgG-HRP as the detection antibody. In the invention, the IgG level of the blood plasma of 30 tree shrews is detected by using the method, wherein according to the detection, the normal IgG level of the blood plasma of the tree shrews is 8.6g / L-17.8g / L. The tri-antibody sandwich ELISA detection method of the IgG of the tree shrew provides necessary tools for the research on the tree shrew as the animal model for the experiment on human diseases and the immunity mechanism of the relates diseases.

The invention discloses a mouse anti-human IgG monoclonal antibody. The monoclonal antibody has amino acid sequences of heavy chain variable regions CDR1, CDR2 and CDR3 as shown in SEQ ID NO.26, 28 and 30, and amino acid sequences of light chain variable regions CDR1, CDR2 and CDR3 as shown in SEQ ID NO.42, 44 and 46. The invention also provides a nucleic acid molecule for encoding the monoclonalantibody, an expression vector containing the nucleic acid molecule and a host cell. The monoclonal antibody disclosed by the invention has relatively high sensitivity and specificity when being applied to immunodetection.

This invention discloses one method and special agent case to test horse head virus antibody, which comprises the following steps: the horse head virus nuclear shell gene expression product of polyphenylacetylene standard board; reaction board adding the horse blood serum to be tested; adding enzyme label IgG single clone antibody, color development and testing for OD490nm. This invention applies real nuclear expression system to re-create bar virus infected insect cell and to get the virus expression.

The invention discloses an immunofluorescence quantitative detection kit for swine reproductive and respiratory syndrome antibodies and a use method thereof. The kit consists of a test paper card, a diluent and a standard product, wherein the test paper card consists of a sample pad, a combination pad, a nitrocellulose membrane and a water suction pad which are sequentially and mutually in lap joint on a PVC plate; a mixture of glycoprotein (GP5 protein) and a recombinant PRRSV capsid protein (N protein) used as a detection line and a goat anti mouse IgG antibody used as a quality control lineare coated on the nitrocellulose membrane; an anti-swine IgG monoclonal antibody marked by rare earth fluorescent microspheres is coated on the combination pad. The detection kit disclosed by the invention uses an indirect method for realizing the quantitative detection of the antibody level in blood serum; detection antigen uses self-made mixed solution of recombinant N protein and recombinant GP5 protein as an antigen pool. Compared with a kit using a single antigen as the detection antigen, the kit has the advantage that the sensitivity is obviously improved. Compared with conventional ELISA, the kit has the advantages of high specificity, high sensitivity, high repeatability and high stability.

The invention relates to a preparation method of a monoclonal antibody of resisting treeshrew IgG, which specifically relates to the field of a monoclonal antibody and separation and purification of treeshrew IgG protein and belongs to the technical field of biology. The preparation method comprises the preparation steps: separating and purifying treeshrew IgG; immunizing a BALB / C rat; combining hybridoma; screening the hybridoma; cloning the hybridoma; preparing ascites of the monoclonal antibody; detecting the valence of the ascites of the monoclonal antibody; and preparing the monoclonal antibody.