103 results about "L-theanine" patented technology

The invention provides a tablet candy and a producing method thereof. The tablet candy is made of the following raw materials according to weight percent: 73.9-85% of sorbitol, 2-5% of flaxseed powder, 0.1-0.3% of L-theanine, 0.1-0.5% of gamma-aminobutyric acid, 0.5-2% of phosphatidylserine, 5-10% of xylitol, 0.1-0.3% of sucralose, 0-5% of flavoring essence, 0-0.1% of colorant, and 1-3% of magnesium stearate. In the tablet candy of the invention, flaxseed powder, L-theanine, gamma-aminobutyric acid and phosphatidylserine interwork according to certain proportion and in synergy, promote mutually, can effectively relieve pressure, tranquillize mood, calm sentiments, and improve memory and thinking capability, so that the tablet candy has health care functions of relieving pressure, relaxingmood, enhancing memory and the like, and is suitable to be eaten by common people as well as diabetics, obese people or people hoping to reduce weight.

The invention discloses health-care medicinal granules capable of relieving mental pressure and resisting depression. The medicinal granules mainly comprises the following nutritional ingredients: Inca Inchi oil, Inca Inchi polypeptide, plantain powder, soybean polypeptide, L-theanine, passionflower, kendir, licorice root, kudzu root, schisandra fruit extract, deep sea fish oil, tryptophan, tyrosine, folic acid and the like. The method comprises the following steps: carrying out cold pressing and enzymolysis, evenly mixing according to parts by weight, adding emulsifier glycerol monostearate,homogenizing and carrying out spray drying. The nutritional medicinal granules disclosed by the invention have good mouthfeel, and has favorable action on improving depressed people with irritability, great mood fluctuations, obvious energy reduction, causeless continuous fatigue, insomnia, early sleep, hypersomnia, and concentration difficulty or plummet. Compared with products which can only relieve pressure and resist depression or western medicines with side effect, the nutritional medicinal granules have obvious advantages, are a novel health-care solid beverage, and have wide market development and application prospects.

The invention provides a method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves and belongs to the technical field of fast tea leaf pesticide detection. According to the method, acetonitrile is adopted for extracting active ingredients in the tea leaves, extract liquor comprises the ingredients of the tea leaves and the pesticide ingredients contained in the tea leaves, ferroferric oxide nanometer particles and graphitized carbon are used for removing fluorescent materials such as chlorophyll and L-theanine in the extract liquor so as to improve the accuracy for detecting the surface enhancing Raman spectrums of the pesticide residues in the tea leaves, and the purpose of fast detecting the pesticide residues in the tea leaves can be achieved. According to the method for fast detecting the surface enhancing Raman spectrums of the pesticide residues in the tea leaves, sample pretreatment is simple and fast, detecting a single sample only needs about 15 minutes, the pesticide residues in tea leaf samples can be analyzed fast and accurately in a quantitative mode, and the method can be used for fast detecting the samples on site in a large-scale mode.

The invention relates to a method for synthesizing theanine, which comprises the following steps: aminating L-N-acyl glutamic acid-gamma-ester in 70-percent ethylamine aqueous solution to obtain L-N-acyltheanine; hydrolyzing the L-N-acyltheanine by adopting L-aminoacylase to remove the acyl group of the L-N-acyltheanine; and performing separation in ethanol aqueous solution to obtain high-quality L-theanine. The method combines the advantages of industrialized acyl hydrolyzation by the L-aminoacylase, uses cheap acyl group as a protective group, and uses the L-aminoacylase to hydrolyze the acyl group after reaction with the ethylamine aqueous solution to obtain the L-theanine. The method has the characteristics of low raw material price, low production cost, high selectivity, high reaction speed and high yield. The used raw materials and reagents are suitable for industrial production, the operation process is simple, and the product quality can be ensured.

The invention discloses a method for synthesizing L-theanine through an enzyme process, and belongs to the biotechnical field. The method is characterized in that a gamma-glutamyltranspeptidase gene is obtained through chemical synthesis, a gene engineering bacterium over-expressing gamma-glutamyltranspeptidase is constructed by treating Escherichia coli as a host bacterium, glutamine and ethylamine hydrochloride having different concentrations are acted by a recombinase, and theanine is efficiently produced at a temperature of 37-50DEG C under a pH value of 9.5-10.5. The enzyme source preparation process has the advantages of simplicity, low cost, and large enzyme amount, and the theanine production method has the advantages of simplicity, high conversion rate, high output, short time and the like, and is in favor of the industrialized amplification production.

A process for synthesizing theanine features that L-pyrrolidone carboxylic acid and anhydrous ethylamine directly take part in reaction in inert gas atmosphere at 60-100 deg.c and 6-12 MPa to obtain L-theanine. Its advantages are high output rate up to 38%, and short reaction time.

The present invention provides a non-addictive composition for the treatment of sleep disorders and problems including melatonin, valerian root extract, an antioxidant component, and a vitamin complex component. The present invention further provides a non-addictive composition for the treatment of sleep disorders and problems including melatonin, valerian Root Extract, proanthocyanidin, vitamin E, vitamin B3, vitamin B6, vitamin B12, L-theanine, and magnesium. The present invention also provides a method for treating sleep disorders or problems of a human including administering a non-addictive composition for the treatment of sleep disorders or problems comprising a melatonin, valerian root extract, an antioxidant component, and a vitamin complex component; and causing the circadian rhythm of the human to be normalized and regulated, causing the sleep patterns of the human to be healthy and normal, and causing reduction of stress and anxiety in the human.

The invention discloses a brain-strengthening and intelligence-benefiting nutritional composition. The brain-strengthening and intelligence-benefiting nutritional composition comprises the following raw materials in percentage by mass: 5-30% of phosphatidylserine, 1-10% of phospholipids, 1-10% of walnut peptides, 0.1-5% of DHA algae oil, 1-15% of L-theanine, 1-15% of sialic acid, 1-10% of a poriacocos extract, 1-10% of a dried orange peel extract, 1-10 % of a sharpleaf galangal fruit extract, 1-10% of a perilla fruit extract, and 1-10% of a saffron extract. The invention further provides a usage of the brain-strengthening and intelligence-benefiting nutritional composition. The brain-strengthening and intelligence-benefiting nutritional composition provided by the invention takes new-resource foods as main raw materials, and is prepared by performing matched blending with extracts of plants which have the functions of strengthening the brain and benefiting intelligence and are used asboth medicines and foods; and thus, the raw materials synergistically take an effect so as to increase brain cell viability of people with excessive brain-strain and / or the elderly, as well as improve the conditions of dizziness,headache and the like caused by overuse of the brain.

The invention discloses a health food used for improving the sleeping quality and a production process thereof. The health food used for improving the sleeping quality consists of a main material and an auxiliary material, wherein the main material is a composition of gamma-aminobutyric acid, L-theanine, spina date seeds, herb of passionflower, piper methysticum forst, humulus lupulus, nepeta cataria and lavender, and the auxiliary material is a composition of sorbitol, mannitol, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, whole milk powder, stevia, arhat fruit extract and food essence. Through the mode, according to the health food used for improving the sleeping quality and the production process thereof, the multiple natural ingredients are adopted, so that after being eaten, the health food can be used for effectively improving the sleeping quality, is high in safety, cannot cause bad stimulation for a human body, and is suitable for being taken for a long time.

The invention relates to the technical field of bioengineering and in particular relates to a synthetic method of L-theanine. The method comprises the following step of synthesizing L-theanine by taking gamma-glutamyl-methylamine synthetase and phosphokinase as catalysts and L-sodium glutamate and ethylamine hydrochloride as substrates. The method can be used for producing a lot of cloned expression proteins as catalysts by taking L-sodium glutamate and ethylamine hydrochloride which are relatively low in price as the initial raw materials, and has the advantages of being mild in reaction condition, strong in specificity, high in reaction conversion rate, simple and convenient to operate, low in requirement on reaction equipment, small in environmental pollution and the like.

The invention belongs to the field of biotechnology, in particular relating to L-theanine enzymatic transformation preparation method. The preparation method takes L-glutamic acid-gamma-alkyl ester or L-glutamyl hydrazide and ethamine as raw materials, mixes bacteria cell containing gamma-glutamyltranspeptidase or enzyme extracting solution with L-glutamic acid-gamma-alkyl ester aqueous solution or the mixed aqueous solution of L-glutamyl hydrazide and ethamine, carries out enzymatic reaction under 25-60 DEG C, and separates the converted product by isoelectric point crystallization or by combining isoelectric point crystallization with ion exchange resin to obtain L-theanine with high purity. The method takes L-glutamic acid-gamma-alkyl ester or L-glutamyl hydrazide as raw material and has the advantages of low price of raw materials, convenient operation, short time of transformation and low preparation cost.

The invention provides a genetic engineering bacterium for L-theanine production and a fermentation method thereof. A construction method of the genetic engineering bacterium comprises the steps of carrying out single copying on an ribonucleic acid (RNA) polymerase gene T7RNAP derived from T7 phage on an Escherichia coli W3110 genome, wherein the gene is controlled by a xylose promoter; carrying out dual copying on a gamma-glutamyl methylamine synthetase gene gmas derived from methylovorusmays, wherein the gene is controlled by a T7 promoter; knocking out a xylose operon repressor protein genexylR; and knocking out a succinyl CoA synthetase gene sucCD. The genetic engineering bacterium has the beneficial effects that the yield of L-theanine fermented by using the genetic engineering bacterium can be up to 40g / L and the sugar acid conversion rate can be up to 25%; and the L-theanine production method provided by the invention has the advantages of being low in raw material price, shortin cycle, simple and convenient to operate, green and friendly to environment and high in yield and has good industrial application value.

The invention belongs to the technical field of genetic engineering, and particularly relates to novel high-efficiency gamma-glutamyl methylamine synthetase and a plasmid-free genetic engineering bacterium for L-theanine production and construction and application thereof. The plasmid-free genetic engineering bacterium which performs denovo synthesis on L-theanine efficiently by taking cheap carbon sources such as glucose as a substrate is provided, escherichia coli serves as a host, and gamma-glutamyl methylamine synthase genes gmas-Mu copied three times are integrated on a genome of the escherichia coli; a glutamate dehydrogenase gene Cgl2079 is copied once; a pyruvate carboxylase gene Cgl0689 is copied once; a citrate synthase gene gltA is copied once, and the genetic engineering bacterium is obtained. After metabolic transformation of a system, the engineering bacterium can perform denovo synthesis on the L-theanine by taking the glucose as the raw material, the fermentation yieldand sugar-acid conversion rate are the highest values reported so far, in fermentation of a 5 L fermentor, the maximum production of the L-theanine can reach 60 g / L, and the sugar-acid conversion ratecan reach 40%.

Embodiments of the present invention relate to a nutritional sleep supplement having a balanced ratio of nutritional elements to facilitate a healthy lifestyle. In one embodiment, a nutritional sleep supplement has a set of active ingredients, the active ingredients comprising: between 50 mg to 600 mg of 5-Hydroxytryptophan; between 125 mg to 1500 mg of calcium lactate; between 50 mg to 600 mg of citric acid; between 125 mg to 1500 mg of gamma-Aminobutyric acid; between 50 mg to 600 mg of L-theanine; between 25 mg to 300 mg of L-tryptophan; between 45 mg to 540 mg of magnesium glycinate; between 1.25 mg to 15 mg of melatonin; between 8.75 mg to 105 mg of niacin; between 7.5 mg to 90 mg of potassium sorbate; and between 10 mg to 100 mg of pyridoxine.

The invention discloses a catalytic synthesis method of L-theanine by using a microorganism-produced gamma-glutamyl amino carboxamide synthase. The catalytic synthesis method comprises the following steps of: screening from tea tree rhizosphere soil to obtain gamma-glutamyl amino carboxamide synthase-sourced strain, nearly Sporidiobolus pararoseus T-C2, with the preservation number of CCTCC NO: M2012232, so as to obtain a purified gamma-glutamyl amino carboxamide synthase; and performing enzymatic synthesis by using the gamma-glutamyl amino carboxamide synthase to obtain the L-theanine, thereby obtaining a method for synthesizing the L-theanine by using the gamma-glutamyl amino carboxamide synthase with high enzyme activity. The synthesis efficiency is much higher than that of the existing enzymatic synthesis technology of the L-theanine by using a microbial enzyme; the safe, simple, convenient, efficient and economical production is realized; and a theoretical basis and a practical basis are provided for subsequent large-scale microbial enzymatic fermentation production of the L-theanine.

The invention relates to a method for synthesizing theanine, which consists of ammonifying the L-N-acyl aminoglutaric acid-ª†-ester with aminoethane solution of 70% and getting L-N-acyl theanine, hydrolyzing with L-aminoacylase, and only L-N-acyl theanine is hydrolyzed to remove the acyl, and evoluting in alcohol solution to get the L-theanine of high quality. The invention combines the dominance of already industrialized L-aminoacylase hydrolyzing acyl, employing cheap acyl as protective group and reacting with aminoethane solution, then hydrolyzing acyl with L-aminoacylase to get L-theanine. The invention is characterized by the cheap raw material, low production cost, high selectivity, fast reaction speed and high productivity. The raw material and agent is suited for industrial production, the operation process is simple and the product quality can be guaranteed.

A liposomal encapsulated solution of L-theanine is sprayed onto the sublingual membrane of a subject. The method may be used in the treatment of stress, tension headaches, attention deficit disorder (ADD), attention deficit disorder hyperactivity (ADHD), claustrophobia, hyperactivity, and anxiety.

The invention relates to a method for synthesizing L- theanine. It takes L- pyrrolidone acid and waterless ethlamine as raw material, carrying out reaction under anti- oxidizing agent existence and with air discharged by dried ethlamine gas condition. The reaction temperature is 30- 59 Deg. C, the pressure is 0.4- 5.9 Mpa, and the productivity is apparently higher than that of under condition without anti- oxidizing agent and with air.

The invention relates to an Escherichia coli strain for biologically converting to synthesize theanine and an application thereof. The Escherichia coli strain for efficiently converting L- glutamine and ethylamine is Escherichia coli of CCTCC No. M 209166. The strain can prepare theanine synzyme in high yield, can efficiently convert the glutamine and the ethylamine into L-theanine and obtains success in the industrial production process. The converting ratio of the glutamine exceeds 80%.

A nutritional supplement includes a predetermined amount of an ATP increasing herbal component which includes species of cordyceps to increase a long-term internal ATP energy; a predetermined amount of L-theanine to increase a mental energy; and a predetermined amount of external energy stimulants including caffeine to provide a short-live external energy, which may be utilized as beverages, foods, supplements, and drugs so as to provide the absolute energy for all mammals including human beings, and pets such as dogs, cats, and rodents.

The present disclosure is a composition, a method of making the composition and method of using such composition preferably in the form of a dietary supplement that, when administered, is capable of treating insomnia and other sleep-related disorders. The unique combination of the composition is preferably administered orally. The composition is preferably comprised of at least vitamin B6, chamomile extract, passionflower extract, lemon balm extract, L-Theanine, GABA, 5-Hydroxytryptophan, and melatonin, in pre-determined amounts. The composition can further comprise a palliative agent, and can be provided in the form of a capsule, powder, liquid or tablet.

The invention relates to the technical field of health-care foods, in particular to a health-care composition and health-care food having the efficacy of improving sleep and / or resisting depression. The health-care composition is prepared from 1-20 parts of GABA-aminobutyric acids, 1-30 parts of L-theanine, 1-50 parts of a gastrodia elata extract and 1-80 parts of a radix morinda officinalis extract. The health-care composition disclosed by the invention has the technical effects that the GABA-aminobutyric acids, the L-theanine, the gastrodia elata extract and the morinda officinalis extract cooperate with each other for use, so that the health-care composition has significant synergistic effects in the respect of the efficacy of improving sleep and / or resisting depression.

The invention discloses a crab fodder capable of improving immunity. The crab fodder is characterized by comprising the following raw materials in parts by weight: 5-8 parts of corn stigma, 26-34 parts of zymosan, 13-19 parts of whey protein concentrate, 18-20 parts of fried gluten puff, 16-19 parts of natto, 14-18 parts of mellea armillaria sporophore, 2-4 parts of ferrous sulfate monohydrate, 3-5 parts of vitamin A, 1-3 parts of vitamin D3, 19-23 parts of shrimp meat, 23-30 parts of pork lib, 2-4 parts of camellia-seed oil, 13-15 parts of broccoli, 17-19 parts of apple, 3.2-4.6 parts of L-theanine, 11-13 parts of a lactobacillus fermentation liquor, 0.3-0.5 part of astragalus membranaceus, 0.4-0.6 part of liquorice, 1-2 parts of endothelium corneum gigeriae galli, 3-6 parts of a phagostimulant and appropriate water. According to the invention, the crab fodder, prepared through the formula disclosed by the invention, can improve the immunity of crabs and promotes rapid growth and anti-stress function of crabs due to the adding of the zymosan, whey protein concentrate and has the virus resisting function due to the adding of codonopsis pilosula, angelica sinensis, L-theanine and the like; the fodder is excellent in palatability and long in water-soaking resistance time; the crabs eating the fodder are high in growth rate and survival rate, and low in disease rate.

The invention provides strong fragrance type baijiu with pressure reduction and blood activating functions and a preparation method of baijiu and relates to the technical field of processing of agricultural products. The strong fragrance type baijiu is prepared from raw materials in parts by weight as follows: 50-60 parts of glutinous sorghum, 20-30 parts of corn flour, 15-20 parts of barley, 3-6 parts of L-theanine, 15-20 parts of distiller's yeast and a proper amount of water. The strong fragrance type baijiu with pressure reduction and blood activating functions contains complete and sufficient nutrition, food materials have the best effect and tastes in a specific formula with a secrete preparation method, the baijiu is clear, transparent, pleasantly fragrant, mellow, sweet and luscious, has strong flavor of baijiu in a mild aromatic flavor, baijiu in a strong aromatic flavor and baijiu in a Maotai flavor, has a refreshing aftertaste, integrates strong aromatic baijiu, Maotai-flavor baijiu and mild aromatic baijiu, has a novel taste, performs the body-building healthcare function, is safe and reliable and has no toxic or side effects.

The invention relates to the field of medical technology. Ethyl 6-bromocoumarin-3-carboxylyl L-theanine, a novel synthetic compound, can inhibit the growth and invasion of cancer cells of human lung cancer, breast cancer, liver cancer, gastric cancer, colon cancer, prostatic cancer, pancreatic cancer, cervical cancer, lymphadenoma, leucocythemia, melanoma, and the like, and can remarkably inhibit the growth and metastasis of the tumors in a body, with an inhibiting effect exceeding the inhibiting effects of anti-cancer drugs. The mechanism of action involves the down regulation inhibition of the bonding of the receptors which are closely related to growth, invasion and metastasis of the tumors, signal transduction regulation and control proteins, the kinase level and a nuclear factor with DNA, and also upper regulation of factors such as cancer-inhibiting proteins and cell cycle inhibition proteins. The ethyl 6-bromocoumarin-3-carboxylyl L-theanine can directly inhibit the activity of histone deacetylase, the activity of histonemethylferase EZH2, and combination of a inflammatory factor NF-[Kabba]B and DNA. The activity of the ethyl 6-bromocoumarin-3-carboxylyl L-theanine exceeds the activity of the anti-cancer drugs. The invention provides new applications of the ethyl 6-bromocoumarin-3-carboxylyl L-theanine for prevention and treatment of diseases such as tumors, inflammation, heart and cerebral vessels and immunodeficiency.

The invention relates to the field of dairy product processing, in particular to yoghourt added with L-theanine and a preparation method thereof. Relative to 100 parts by weight of raw materials, the yoghourt added with the L-theanine comprises 0.3-0.8 part of L-theanine, 0.03-1 part of stabilizer, 0.005-0.009 part of lactobacillus leaven and the balance of milk, wherein the content of the L-theanine is 0.5 part, the stabilizer contains 0.03-0.1 part of pectin and 0.3-1 part of starch; or the stabilizer contains 0.03-0.1 part of pectin, 0.3-1 part of starch and 0.1-0.5 part of gelatin. Through the reasonable mixing of the L-theanine, the stabilizer and other flavoring agents and the combination of process conditions, the yoghourt added with the L-theanine which can be refrigerated for more than 21 days at 2-6 DEG C is produced and has favorable mouth feel and flavor.

The invention relates to a cigarette addiction eliminating chewing gum, and more specifically relates to chewing gum that can gradually eliminate a cigarette addiction, and also can reach the purposes of heart-fire clearing, refreshing, and work efficiency improving. In the invention, two active ingredients are added into the chewing gum, wherein one ingredient is a green tea extract L-Theanine and the other is an Ampelopsis grossedentata extract Dihydromyricetin. The L-Theanine can eliminate the cigarette addiction, clear heart-fire and refresh people, while the Dihydromyricetin can provide health care to a plurality of human body internal organs including lungs.

Disclosed are compositions of matter useful for treating anxiety and depression. In one embodiment a nutraceutical composition is administered to a patient in need of said composition comprising of the following combination of ingredients: niacin, magnesium, ashwagandha, passionflower, skullcap, St. John's Wort, gamma aminobutryic acid, chamomile, and L-theanine

The invention discloses a composite microorganism beverage and a preparation method thereof, and particularly discloses a composite microorganism beverage for treating high blood pressure and coronary heart disease and concurrently preventing and treating constipation and diarrhoea, and a preparation method of the composite microorganism beverage. The composite microorganism beverage is prepared from the following raw materials of streptomyces ogaensis fermentation liquor, clostridium butyricum fermentation liquor, saccharomyces boulardii fermentation liquor, bacillus laevolacticus fermentation liquor, lactobacillus brevis fermentation liquor, soybean flour, brown sugar, L-theanine, xylooligosaccharide and purified water. The microorganism beverage disclosed by the invention integrates treatment health-care functions of various single products, not only contains microorganism groups and metabolism products for preventing and treating the coronary heart disease and the high blood pressure, but also has microorganism groups and metabolism products for regulating the internal balance of the stomach and the intestine, and preventing and treating constipation and diarrhoea, integrates health-care and treatment functions, conforms to the current medicine viewpoint of people for combining prevention, health care, treatment and recovery, and is quite wide in market prospects.

The invention discloses a method for efficiently synthesizing L-theanine through recombined corynebacterium crenatum, and belongs to the field of genetic engineering and enzyme engineering. According to the method, it is firstly verified that GGT signal peptide of a bacillus subtilis source can achieve secretory expression in a C.glutamicum system. Meanwhile, extracellular enzyme activity of recombinant bacteria C.glutamicum SDNN403 / pXMJ19-tacM-ggt is about two times that of C.glutamicum SDNN403 / pXMJ19-ggt, and it indicates that a tac Mpromoter is more beneficial for synthesis of GGT enzyme activity. Substrate flow and strategic high-yield L-theanine are adopted, a conversion system contains GGT with the concentration of 0.8 u / mL, the pH is 10, the temperature is 37 DEG C, and 22 mmol / L L-theanine and 66 mmol / L ethylamine are supplemented every other two hours from 0 h. The maximum theanine yield of 118 mmol is achieved when batch flow is added for 12 h, and the conversion rate is 92.8%. The secretory expression of gamma-glutamyltranspeptidase genes (ggt) of the B.subtilis source in C.glutamicum SDNN403 is achieved for the first time, and the highest yield of L-theanine reported currently and synthesized through recombined C.glutamicum is obtained by adding the substrate in batch flow.