183 results about "Limonin" patented technology

The invention discloses a lemon fermented wine and a preparation method thereof. The preparation method comprises the following steps: preparing the activated conventional wine-brewing dry yeast intoactive lemon yeast strain liquid, adding into lemon fruit juice and fermenting; preparing lemon raw wine obtained by fermentation into lemon fruit wine by ageing, clarifying, freezing and filtering; and preparing the lemon raw wine into lemon spirit by distilling. The invention solves the problem of growth of common fruit wine yeast in a lemon fermentation environment. The prepared lemon fruit wine well retains natural nutritional health-care components and health-care effect in lemon, has unique taste, mellowness and fragrance, integrates nutrition, health care and naturalness into a whole and is also more beneficial for a human body to absorb. The product is rich in organic acids, limonin class substances, flavonoid and terpenoid substances, has the functions of diminishing inflammation,suppressing tumours, resisting fatigue, activating blood vessels and promoting the digestion of the human body and also has the auxiliary effects of cleaning the bowel and the stomach, lowering cholesterol, enhancing appetite and the like. The lemon distilled wine prepared by distilling the fruit wine has sweet, clear, cool, mellow and soft mouth feel, has the specific fragrance of the lemon andalso has the style of brandy.

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

The invention provides a process for extracting limonin from a shaddock kernel. The process is characterized in that the process comprises the following steps that: (1) pre-treatment of shaddock kernel: after washing and drying, the shaddock kernel is crushed to be between 20 and 40 meshes; (2) extraction: by taking a water solution of ethanol as a solvent, the shaddock kernel powder is subjected to thermal refluxing extraction at a temperature of between 90 and 100 DEG C; (3) impurity separation: the obtained product after thermal refluxing extraction is orderly impurity-separated by macroporous occluded resin, leached by 30 to 50 percent water solution of ethanol and eluted by 60 to 80 percent water solution of ethanol, and the leaching liquid is abandoned, and the eluate is collected; and (4) the collected eluate is condensed until being dry and dissolved by methylene chloride and filtered; the obtained product is added with isopropyl alcohol which is doubled in volume than the obtained product, cooled down to the temperature of 4 DEG C, and slowly stirred for crystallization; and the crystal is filtered and dried to obtain the finished product. The process has the advantages that by utilizing the processes of thermal refluxing extraction, impurity separation by occluded resin, and the like, the limonin with the content of over 90 percent can be extracted from the shaddock kernel; moreover, the process also has the advantages of simple process, recoverable leaching agent, low cost and mass production.

The invention discloses a method for continuously extracting orange oil and limonin from citrus reticulate by using supercritical carbon dioxide, and belongs to the fields of grease extraction methods and natural product separation. A process flow comprises the following steps of: drying and crushing citrus reticulate tangerine seeds; performing supercritical extraction; separating mandarin oil from residues; performing supercritical extraction; separating; and crystallizing and purifying to obtain the limonin. The method has the advantages of high product yield and no solvent residue. By continuously extracting the orange oil and the limonin from the tangerine seeds, full utilization of the waste citrus reticulate tangerine seeds is realized, the high-quality orange oil and high-purity limonin are obtained, the production cost is reduced, and the added value and the economic benefit of a product are improved.

The invention relates to a high performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of a fructus evodiae formula granule. The method is characterized in that a HPLC-MS technology is used; acetonitrile is taken as the A phase; acetic acid (0.12%)-ammonium acetate (1 mmol / L) is taken as the B phase; gradient elution with four scale transformations is performed; the flowing speed is 0.8 mL / min; the column temperature is 40 DEG C; in a multiple reaction monitoring (MRM) mode, positive ions and negative ions are monitored at the same time, the ion source is an ESI source, the atomized gas (GS1) is N2, the auxiliary gas (GS2) is N2; nitrogen gas is used during the whole process; the collision gas pressure is medium; the resolutions of Q1 and Q3 are both UNIT; the method can detect 10 components in the fructus evodiae formula granule, namely chlorogenic acid, caffeic acid, rutin, hyperin, hesperidin, dehydroevodiamine, limonin, evodiamine, rutaecarpin, and evocarpine; 10 components can be all detected in 22 minutes, and the method is rapid and accurate and is not disturbed.

The invention relates to the technical field of plant separation and extraction, and in particularly discloses a method of combined extraction of essential oil, pectin, hesperidin, synephrine and limonin from citrus. The method comprises the following steps: inactivating the citrus, buffing and performing enzymolysis to obtain essential oil, a water phase and skin residues; separating the water phase through strong acidic cation-exchange resin to collect effusive liquid, and eluting to obtain synephrine; separating the effusive liquid using ultra-filtration method to obtain pectin and liquid passing through the ultra-filtration film; refluxing and extracting the liquid passing through the ultra-filtration film using ethanol to obtain supernatant and filter residue; crystallizing, and freeze-drying the filter residues to obtain aurantiamarin; separating the supernatant using macroporous resin, and eluting using ethanol to obtain limonin. The method is used for processing fresh citrus to co-produce the citrus pericarp essential oil, pectin, hesperidin, synephrine and limonin; the utilization rate of the resource is improved, the environment pollution is reduced, and the economic and social benefits are notable.

The invention relates to a method for extracting limonin substances from citrus limon seeds as a raw material. The method comprises the following steps: crashing the citrus limon seeds; adding water and an enzyme preparation for enzymolysis; standing; discarding enzymolysis liquid; adding alcohol to sediment for leaching; adopting the absorption chromatographic separation of leaching liquid to obtain the limonin substances; and drying the limonin substances. The method uses lemon product waste, i.e. lemon rods as the raw material so as to not only fully utilize the waste generated in the production process, lessen the waste of the raw material and reduce the environmental pollution, but also obtain the limonin substances with high content up to 95 percent and extraction rate up to 90 percent which is higher than that of the prior extraction rate by 30 percent, and adopts ethanol digestion, macroporous resin column chromatographic separation and silica gel chromatographic column purification to decrease the production cost.

The invention belongs to the field of the extraction of natural medicines and particularly relates to a limonin analogue and a separation and purification method thereof. The invention aims to solve the technical problem of providing a fire-new separation and purification method of a limonin analogue. According to the separation and purification method disclosed by the invention, the medicinal materials of rue family citrus plants, such as tangerine seeds, pummelo seeds, immature bitter oranges, tangerine peels and the like, are used as raw materials, 40 to 50 percent of ethanol is used as a solvent for extraction, an extract is separated and purified by adopting HPD-722 type macroporous resin, the content of the limonin analogue in obtained solid matters is greater than 80 percent, the content of limonin is greater than 20 percent, and the content of nomilin is greater than 40 percent. The method has high extraction efficiency, little reagent consumption and little pollution and is simple to operate.

The invention provides a preparation method of natural whole-lemon-fruit freeze-dried powder. The preparation method comprises the steps of grinding whole-lemon-fruit and / or peel dregs obtained after juicing at a low temperature to obtain pulp, and sequentially carrying out quick freezing and freeze drying, so as to obtain the freeze-dried powder. The natural whole-lemon-fruit freeze-dried powder is prepared into any one of oral preparations including capsules, granules, tablets, powder or pills. According to the preparation method, aurantiamarin, naringin and lemon essential oil which have the functions of reducing blood fat and blood pressure in lemon peels are furthest remained, and limonin substances which have an anti-cancer function in lemon seeds are furthest remained; a traditional eating manner is changed, the natural whole-lemon-fruit freeze-dried powder is natural, easy to carry and high in fruit utilization rate, and the effect of directly eating fresh lemons can be achieved by eating a preparation prepared from the freeze-dried powder.

The invention relates to compositions and methods for preventing, treating, or managing anxiety, pain, chronic pain, depression, and disorders such as premenstrual syndrome comprising the administration of a prophylactically and therapeutically effective amount of Rutaceae plant or extracts thereof to a mammal in need of such therapy. In a preferred embodiment, the mammal is human and the extracts are substantially free of the compounds obacunone or limonin. The invention also relates to compositions and methods for preventing, treating, or managing separation anxiety in domestic animals comprising the administration of a prophylactically and therapeutically effective amount of Rutaceae plant or extracts thereof to an animal in need of such therapy.

The invention provides a method for preparing a composition comprising high-purity limonin and a medicinal composition which is prepared by the method, has high safety and comprises the high-purity limonin. In the preparation method, the limonin of which the purity is over 98 percent can be obtained under the condition of recrystallization and purification for two times; and the impurities in the limonin do not damage the safety. Moreover, the invention also provides application of the medicinal composition in preparing a medicament for preventing and / or treating the colon cancer or the rectal cancer.

The present invention discloses a method to remove bitterness of the orange juice using a compound enzyme preparation, which comprises the following steps. Step a is that the orange juice is obtained by crude filtration after juice pressing. Step b comprises obtaining a compound enzyme preparation from a dictamnolactone enzyme and a naringinase with the weight ratio between five to 1 and one to five. Step b comprises adjusting pH value of the orange juice, adding the compound enzyme preparation with the addition quantity of 1g per kilogram to 3g per kilogram, heating the juice till 30 DEG C to 75 DEG C, keeping the constant temperature for 20 minutes to 90 minutes. Step d comprises obtaining the orange juice after minutes, enzyme denaturing and sterilization through instantaneous heating by super high temperature. Characteristics of the invention method comprises little nutrient loss and little loss in orange juice flavor, good debittering effect, stable product quality, high degree safety in the product, high production efficiency, simple operation and convenient application.

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+ / -0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

The invention provides a process for preparing limonin monomer by an industrial chromatography method. The process comprises the following steps: extracting oil from citrus seeds after being washed and dried by alcohol organic solvent, filtering and separating to obtain filtrate; decompressing and concentrating the filtrate, recycling the alcohol organic solvent, extracting by hydrochloric ether and concentrating to form extract; refluxing and extracting the extract by ether solvent, degreasing the extract, obtaining filtration residues by filtering and washing the filtration residues until no fatty acid exists; adding alcohol solvent into the filtration residues, carrying out recrystallization to obtain coarse crystal containing a plurality of limonin compounds, crystallizing and drying to obtain limonin compound crystal after carrying out the chromatography by an industrial chromatographic column. The process fully utilizes waste citrus seeds and adopts the industrial chromatography method to extract high-purity limonin, improves the comprehensive utilization ratio, has low cost, easy operation and control, repeated solvent utilization and reduced pollution and is suitable for industrialized large-scale production.

The invention discloses a method for debitterizing lemon juice, belonging to the technical field of beverage preparation. The method comprises the steps as follows: firstly, selecting an undamaged lemon, cleaning, peeling, juicing, filtering and centrifuging to obtain the lemon juice; secondly, putting the lemon juice into a water bath kettle being 85-95 DEG C, deactivating enzyme for 8-15min, and cooling; thirdly, measuring the bitter substance content and the Vc content of the lemon juice; fourthly, adding 0.3-0.7g of beta-cyclodextrin into every 100mL of the lemon juice subjected to enzyme deactivation, uniformly mixing, putting a mixture into the water bath kettle, and processing for 60-120min at the temperature of 30-50 DEG C; and lastly, measuring the bitter substance content and the Vc content of the debitterized lemon juice, and calculating the debitterizing rate and the loss rate of the lemon juice. According to the method provided by the invention, the removal rate of bitter substances-limonin and naringin in the lemon juice is high, the loss rate of Vc is low, and the lemon juice with good flavor can be obtained; and meanwhile, the cost is low, the required equipment is simple and the processing operation is convenient.

The invention discloses a fermentation production method of a grapefruit juice debitterizing enzyme and a using method thereof, and the main technical process comprises the following steps: extracting bitter substances from grapefruit; taking the bitter substances of the grapefruit as a sole carbon source for preparing a culture medium; inoculating aspergillus niger for fermentation and producingthe grapefruit juice debitterizing enzyme; extracting the grapefruit juice debitterizing enzyme; carrying out enzymatic hydrolysis on the bitter substances in grapefruit juice; and concentrating for preparation and obtaining the concentrated juice. The grapefruit juice debitterizing enzyme fermented by the invention comprises naringinase, limonin enzyme, nomilin enzyme and other enzyme systems, and can specifically decompose the bitter substances in the grapefruit juice, be used for debitterizing the grapefruit juice and be capable of obtaining the grapefruit juice which completely removes the bitterness. The method does not affect the nutrition and the flavor of a product, has a wide range of technical applications, and can not only be used for production of the grapefruit juice productswith different uses, but also be used for carrying out the debitterizing treatment on grapefruit peels and fruit residues.

The invention discloses a method for removing the bitter taste of lemon juice with a macroporous resin, belonging to the technical field of drink preparation. The method comprises the following steps: selecting ripe and undamaged fresh lemons and washing, peeling and juicing the fresh lemons and filtering the juice to obtain the lemon juice; deactivating the enzyme of the lemon juice at 85-95 DEG C for 8-15 minutes and cooling the lemon juice; soaking the macroporous resin LS-803C in absolute ethyl alcohol, washing the macroporous resin LS-803C with an acid and an alkali and removing the impurities and then packing a column with the macroporous resin LS-803C; and passing the treated lemon juice through a resin column with height-diameter ratio of (5:1)-(20:1) at a flow rate of 2-11BV / h at a room temperature of 20 DEG C, thus obtaining the debitterized lemon juice. The method has the beneficial effects that the rates of removal of bitter substances in the lemon juice, especially naringin and limonin are respectively higher than 90% and 60%; the used resin can be reused, so that the processing cost is relatively low and the method is convenient to operate; the two bitter substances, namely naringin and limonin adsorbed by the resin can be further separated, purified and recycled.

The invention relates to a process for hierarchically extracting limonin, flavonoid and dietary fiber from orange peel residue, and the orange peel residue is used as a raw material. According to different properties and characteristics of limonin, flavonoid and dietary fiber to be extracted, different methods and technical processes are adopted after primary production to avoid mutual interference among the contents and hierarchically and sequentially extract limonin, flavonoid, soluble dietary fiber and insoluble dietary fiber. Therefore, the invention achieves purpose of decreasing the cost, improving the efficiency, simplifying the process and respectively extracting a plurality of contents.

The invention discloses a method for debittering citrus juice. The method comprises the following steps of: a) destroying a colloid system in the juice by a hydrolysis method or a pH value control method; and b) centrifugally separating bitter substances out under the low temperature condition, and thus obtaining the juice with low bitter substance content. According to the hydrolysis method, pectin or plant fibers in the juice is or are hydrolyzed by pectinase or cellulose. According to the pH value control method, the pH value of the juice is reduced by adding an acid solution. According tothe method, the colloid system of the juice is destroyed, and the bitter substances, in particular limonin type bitter substances, are separated from the juice by a low temperature centrifugal method, so that a debittering effect is achieved.

The invention discloses a novel limonin compound as well as a preparation method and a medical application thereof. The compound which is reported for the first time is a limonin compound having a novel structure and can be obtained by extracting, separating and purifying from dried roots of attalanteae buxifoliae. In-vitro experiments prove that the compound has the capabilities of reducing ischemia reperfusion injury of human hepatocytes and playing a role of hepatic cell protection and can be used for being developed into medicaments for protecting the liver.

Extraction of flavonoid citrus extract from the by-product of a juice extraction process, to provide a flavonoid-rich citrus extract is disclosed. The extraction process reduces the level of limonin and / or bitterants in the by-product extract without affecting the level of naturally occurring flavonoids, which may be added to citrus-base or non-citrus-based beverages, or used as a stand-alone juice.

The invention provides grapefruit brandy and a preparation method thereof, and belongs to the technical field of wine brewing. The preparation method is characterized in that grapefruit is used as a raw material; before fermenting, fruit pulp is heated, so as to perform sterilizing on one hand and deactivate pectin on the other hand, so that prepared crude wine can meet a safety standard, namely the content of methanol is smaller than 0.12g / 100mL. By adopting the preparation method, most of beneficial components in the grapefruit can be reserved; the prepared grapefruit brandy is rich in aromatic component, naringin and limonin, and has the particular aroma of the grapefruit is contained; after the grapefruit brandy is drunk, a heavy fruit flavor and slight bitter taste are reserved in anoral cavity, and the bitter taste can be quickly dissipated.

A sample preprocessing method for detecting lemon bitter principle compound content in citrus skin and seed includes drying and grinding detected sample to be 60 ¿C 80 mu, adding 2 ¿C 5 ml organic solvent into ground sample of 0.2 ¿C 0.5g, oscillating and extracting it for 2 ¿C 3 min. then centrifuging it for 5 ¿C 10 min. to absorb off top clear liquid; adding acetone of 2 ¿C 5ml, extracting and oscillating it for 2 ¿C 3 min. then centrifuging it for 5 ¿C 10min. to collect acetone extractive; adding 1¿C2ml of organic solvent into extractive, oscillating it then centrifuging it for 5 ¿C 10min to collect solution for fixing volume and detecting.

The invention discloses a grapefruit pericarp and pomace extractive preparation method and a preparation containing the extractive and belongs to the preparation method of plant active ingredients. The preparation method comprises the following steps: sequentially cutting up, drying and smashing fresh grapefruit pericarp and pomace to obtain powder; using a 95% ethanol solution to perform heating reflux on the powder for extraction so as to obtain an oily extractive; adding petroleum ether into the oily extractive for ungrease treatment so as to obtain a nonfat extractive; utilizing D101 macroporous resin to perform column chromatography on the nonfat extractive, using ethyl alcohol to perform elution, collecting the eluant, and performing rotary evaporation on the eluant to obtain the grapefruit pericarp and pomace extractive. According to the invention, the grapefruit pericarp and pomace waste is used as the raw material to establish the effective ingredient extractive technology, extract limonin and flavonoid substances, research and develop capsula capable of reducing blood fat and meet the demand of the current blood fat reduction health care product market, so that the grapefruit pericarp and pomace extractive has broad market prospects.

The present invention discloses a one-step debitterizing and reducing acidity method of high pulp content juices, and belongs to the technical field of juice preparation. The method includes the following steps: juicing fresh fruits and obtaining a raw juice, centrifuging the raw juice to obtain a crude separation of juice and pulp, pumping the crude separation juice into a buffer tank, pumping the crude separation pulp into a recovery tank for the subsequent backfilling; carrying out high-speed centrifugal separation of the crude separation juice and obtaining a fine separation of juice and pulp, pumping the fine separation juice into the buffer tank, and carrying out sterilizing and inactivating enzyme at a temperature of 85 to 90 DEG C for 10s to 30s, pumping the fine separation pulp into the recovery tank for the subsequent backfilling; after sterilizing and inactivating enzyme, pumping the fine separation juice from the buffer tank to a mixed resin tank, carrying out debitterizing and reducing acidity; backfilling the pulp, carrying out vacuum concentration and obtaining the high pulp content juices. The method can realize debitterizing and reducing acidity in one resin tank, maximize retain juice flavonoids, VC and other nutrients of the juice on the premise of removing limonin and fruit acid, and ensure the juice quality.

The invention discloses a acetobacter pasteurium and its application method in producing citrus vinegars and resulting citrus vinegar products. The invention provides acetobacter pasteurium named LSYY001.2, preserved in CGMCC on 14th June, 2010, preservation number: CGMCC No.4954. Method of the invention for producing citrus vinegar comprises: selecting materials, peeling citruses, removing segment membranes, crushing, filtering, adding yeasts for alcoholic fermentation, adding LSYY001.2 for acetic acid fermentation, then filtering. The acetobacter pasteurium provided by the invention not only ferments alcohol for producing acetic acid, but simultaneously produces iso-limonin hydrolase, thus realizing removing bitter of the citrus vinegar.

The invention discloses a raw material formula of the lemon lozenge and a preparation process thereof. The raw material formula comprises the following raw materials: lemon powder or lemon concentrated solution, excellent powdered sugar or xyllitol, glycyrrhizin, starch, one or more of lemon yellow or sunset yellow and lemon oil. The lemon lozenge solves the problem that the lemon is peracid and diet and cannot be directly eaten, and solves the technical problems for directly performing the fruit powder. The lemon lozenge has the advantages of effective biological activity and easy absorption, and maintains a great amount of nutrients and Vc of the lemon. The product is also a leisure product with low calorie and functions, not only maintains the original unique flavor of the lemon, but also maintains the biological activity and the unique pharmaceutical value of the lemon. The product is rich in vitamins, organic acid, limonoid, flavonoid and hemiterpene substances, can moisten the throat, and can diminish the inflammation. Organic acid can resist the fatigue, activate the blood vessel, and can promote the digestion of the human body, and also has the auxiliary functions for clearing the intestines and stomach, reducing the cholesterol, improving the appetite, and the like.

The invention relates to the field of pharmaceutical chemistry and in particular relates to a type of water-soluble limonin derivatives (I) and (II) and preparation methods thereof. Pharmacodynamic experiment results prove that the compound disclosed by the invention has the effects of treating inflammations, rheumatoid arthritis, pain and the like. The structural formula is as shown in the specification.

The invention discloses an extraction method of limonin. The extraction method comprises the following steps: pretreatment: adding lemon seeds and lemon peel residues into water, carrying out soaking,and adding a pretreatment enzyme to decompose impurities; extraction: adding an extracting enzyme into a pretreatment product for enzymolysis, adding acid for acidolysis, performing solid-liquid separation, removing solid residues, and reserving liquid to obtain a primary product; removal of pectin: carrying out vacuum concentration on the primary product, adding alcohol for alcohol precipitation, separating a floccule precipitate, and reserving liquid; and purification: carrying out extracting at first, and then conducting separating by using macroporous resin. The extraction method disclosed by the invention is reasonable in design, all the steps are associated with one another, impurities are removed one by one, and extraction and macroporous resin separation are combined for use; andextraction is carried out at first and then macroporous resin separation is carried out, and the advantages of two separation means are combined, so separation efficiency is high, and product purity is high.

The invention provides honey pomelo tea prepared by using Liangping pomelos as raw materials, solves the problem that in the processing course of the pomelos, heavy bitter and numbing taste appears, and provides a new way for deep processing of the Liangping pomelos. According to the honey pomelo tea disclosed by the invention, food industry wastes such as peanut shells, peanut coats, longan kernels, grape seeds and chestnut shells are used as main raw materials, through reasonable compounding ratio of the main raw materials with polished glutinous rice, corn stigma, calcium citrate and glycerin, and in connection with the technology disclosed by the invention, a debitterizing agent can be made, and about 50-60% of bitter substances in the Liangping pomelos can be removed. Pomelo leaves, zinc lactate, and nicotinamide can improve the debitterizing effect of the debitterizing agent, and have the synergistic effect on the debitterizing effect of the debitterizing agent, so that the removing rate of naringin and limonin in the Liangping pomelos can achieve 99% or above, besides, nutrient substances of VC, sugar, organic acid and the like in the pomelos can be preserved to the maximum extent, the bitterness is removed, and besides, the loss of nutrient components in the pomelos is reduced.